Publicação
Commelina erecta as a source of natural bioactive compounds
| Resumo: | Plants are an important source for the discovery of new products of medicinal value and unique sources for food additives, flavors, and other compounds with industrial value Commelina erecta L. (Commelinaceae) is an herbaceous flowering plant, popularly known as “trapoeraba” and “erva-de-santa-luzia”. In folk medicine, C. erecta has been used as antiviral, for the treatment of haemorrhages, skin rashes and sores, and for the treatment of infections [1]. Moreover, it is also used as a wild edible plant in salads, preserves or decorating the dishes [1]. The present study aims at evaluating the bioactive potential, namely, antioxidant, cytotoxicity to tumor and non-tumor cell lines, and anti-inflammatory activities of the stem and flower of C. erecta. Antioxidant activity was evaluated in the hydroethanolic extracts by two in vitro assays measuring inhibition of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) assay and antihaemolytic activity (OxHLIA). The cytotoxicity was tested using four human tumor cell lines: AGS (gastric adenocarcinoma), CaCo (colorectal adenocarcinoma), NCI-H460 (non-small cell lung cancer), MCF-7 (breast carcinoma), as well a non-tumor culture from African green monkey (Vero). Anti inflammatory activity was determined based on the nitric oxide (NO) production by a murine macrophage (RAW 264.7) cell line. Significant differences between the evaluated extracts were observed, the hydroethanolic extract showed the best activity in the TBARS assay for the stem sample, with EC50 value of 0.63±0.01 mg/mL. On the other hand, in the OxHLIA assay, flower hydroethanolic extract present an IC50 of 5.1±0.2 μg/mL, value required to protect half of the erythrocyte population from the hemolytic action caused the used oxidative agent at Δt = 60 min. Stem sample presented effective results in the inhibition of the tested tumor cell lines, namely, AGS, CaCo and MCF-7, while flower is more effective against NCI-H460. Both extracts exhibited toxicity against non-tumoral cell lines, denoting that these extracts have cytotoxicity. The in vitro anti inflammatory potential of extracts was evaluated by measuring NO inhibition and the most effective extract was the flower with an EC50 values of 41±1 μg/mL. These results indicate that stems and flowers of C. erecta might be a potential source of natural biomolecules for pharmaceutical and food applications. |
|---|---|
| Autores principais: | Cavichi, Lucas V. |
| Outros Autores: | Fernandes, Ângela; Mandim, Filipa; Pinela, José; Canan, Cristiane; Amaral, Joana S.; Ferreira, Isabel C.F.R.; Barros, Lillian |
| Assunto: | Commelina erecta L Erva-de-santa-luzia |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173317764448256 |
|---|---|
| author | Cavichi, Lucas V. |
| author2 | Fernandes, Ângela Mandim, Filipa Pinela, José Canan, Cristiane Amaral, Joana S. Ferreira, Isabel C.F.R. Barros, Lillian |
| author2_role | author author author author author author author |
| author_facet | Cavichi, Lucas V. Fernandes, Ângela Mandim, Filipa Pinela, José Canan, Cristiane Amaral, Joana S. Ferreira, Isabel C.F.R. Barros, Lillian |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cavichi, Lucas V.\"},{\"Person.name\":\"Fernandes, Ângela\",\"Person.identifier.orcid\":\"0000-0002-0157-9873\"},{\"Person.name\":\"Mandim, Filipa\",\"Person.identifier.orcid\":\"0000-0002-6494-2326\"},{\"Person.name\":\"Pinela, José\",\"Person.identifier.orcid\":\"0000-0001-7523-1637\"},{\"Person.name\":\"Canan, Cristiane\"},{\"Person.name\":\"Amaral, Joana S.\",\"Person.identifier.orcid\":\"0000-0002-3648-7303\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Cavichi, Lucas V. Fernandes, Ângela Mandim, Filipa Pinela, José Canan, Cristiane Amaral, Joana S. Ferreira, Isabel C.F.R. Barros, Lillian |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-01-20T16:11:49Z |
| datacite.date.embargoed.fl_str_mv | 2022-01-20T16:11:49Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Commelina erecta L Erva-de-santa-luzia |
| datacite.titles.title.fl_str_mv | Commelina erecta as a source of natural bioactive compounds |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Cavichi, Lucas V. Fernandes, Ângela Mandim, Filipa Pinela, José Canan, Cristiane Amaral, Joana S. Ferreira, Isabel C.F.R. Barros, Lillian |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-01-20T16:11:49Z |
| dc.date.embargoed.fl_str_mv | 2022-01-20T16:11:49Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/24808 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Commelina erecta L Erva-de-santa-luzia |
| dc.title.fl_str_mv | Commelina erecta as a source of natural bioactive compounds |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Plants are an important source for the discovery of new products of medicinal value and unique sources for food additives, flavors, and other compounds with industrial value Commelina erecta L. (Commelinaceae) is an herbaceous flowering plant, popularly known as “trapoeraba” and “erva-de-santa-luzia”. In folk medicine, C. erecta has been used as antiviral, for the treatment of haemorrhages, skin rashes and sores, and for the treatment of infections [1]. Moreover, it is also used as a wild edible plant in salads, preserves or decorating the dishes [1]. The present study aims at evaluating the bioactive potential, namely, antioxidant, cytotoxicity to tumor and non-tumor cell lines, and anti-inflammatory activities of the stem and flower of C. erecta. Antioxidant activity was evaluated in the hydroethanolic extracts by two in vitro assays measuring inhibition of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) assay and antihaemolytic activity (OxHLIA). The cytotoxicity was tested using four human tumor cell lines: AGS (gastric adenocarcinoma), CaCo (colorectal adenocarcinoma), NCI-H460 (non-small cell lung cancer), MCF-7 (breast carcinoma), as well a non-tumor culture from African green monkey (Vero). Anti inflammatory activity was determined based on the nitric oxide (NO) production by a murine macrophage (RAW 264.7) cell line. Significant differences between the evaluated extracts were observed, the hydroethanolic extract showed the best activity in the TBARS assay for the stem sample, with EC50 value of 0.63±0.01 mg/mL. On the other hand, in the OxHLIA assay, flower hydroethanolic extract present an IC50 of 5.1±0.2 μg/mL, value required to protect half of the erythrocyte population from the hemolytic action caused the used oxidative agent at Δt = 60 min. Stem sample presented effective results in the inhibition of the tested tumor cell lines, namely, AGS, CaCo and MCF-7, while flower is more effective against NCI-H460. Both extracts exhibited toxicity against non-tumoral cell lines, denoting that these extracts have cytotoxicity. The in vitro anti inflammatory potential of extracts was evaluated by measuring NO inhibition and the most effective extract was the flower with an EC50 values of 41±1 μg/mL. These results indicate that stems and flowers of C. erecta might be a potential source of natural biomolecules for pharmaceutical and food applications. |
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| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/24808 |
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| institution | Instituto Politécnico de Bragança |
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| language | eng |
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| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Cavichi, Lucas V. Fernandes, Ângela Fernandes, Ângela https://www.ciencia-id.pt/2015-0434-AE7C 2015-0434-AE7C http://orcid.org/0000-0002-0157-9873 0000-0002-0157-9873 Mandim, Filipa Mandim, Filipa https://www.ciencia-id.pt/F318-9049-EB99 F318-9049-EB99 http://orcid.org/0000-0002-6494-2326 0000-0002-6494-2326 Pinela, José Pinela, José https://www.ciencia-id.pt/771C-2B43-B108 771C-2B43-B108 http://orcid.org/0000-0001-7523-1637 0000-0001-7523-1637 Canan, Cristiane Amaral, Joana S. Amaral, Joana S. https://www.ciencia-id.pt/5319-7DE8-BEDA 5319-7DE8-BEDA http://orcid.org/0000-0002-3648-7303 0000-0002-3648-7303 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 |
| publishDate | 2021 |
| reponame_str | Biblioteca Digital do IPB |
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| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTPlants are an important source for the discovery of new products of medicinal value and unique sources for food additives, flavors, and other compounds with industrial value Commelina erecta L. (Commelinaceae) is an herbaceous flowering plant, popularly known as “trapoeraba” and “erva-de-santa-luzia”. In folk medicine, C. erecta has been used as antiviral, for the treatment of haemorrhages, skin rashes and sores, and for the treatment of infections [1]. Moreover, it is also used as a wild edible plant in salads, preserves or decorating the dishes [1]. The present study aims at evaluating the bioactive potential, namely, antioxidant, cytotoxicity to tumor and non-tumor cell lines, and anti-inflammatory activities of the stem and flower of C. erecta. Antioxidant activity was evaluated in the hydroethanolic extracts by two in vitro assays measuring inhibition of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) assay and antihaemolytic activity (OxHLIA). The cytotoxicity was tested using four human tumor cell lines: AGS (gastric adenocarcinoma), CaCo (colorectal adenocarcinoma), NCI-H460 (non-small cell lung cancer), MCF-7 (breast carcinoma), as well a non-tumor culture from African green monkey (Vero). Anti inflammatory activity was determined based on the nitric oxide (NO) production by a murine macrophage (RAW 264.7) cell line. Significant differences between the evaluated extracts were observed, the hydroethanolic extract showed the best activity in the TBARS assay for the stem sample, with EC50 value of 0.63±0.01 mg/mL. On the other hand, in the OxHLIA assay, flower hydroethanolic extract present an IC50 of 5.1±0.2 μg/mL, value required to protect half of the erythrocyte population from the hemolytic action caused the used oxidative agent at Δt = 60 min. Stem sample presented effective results in the inhibition of the tested tumor cell lines, namely, AGS, CaCo and MCF-7, while flower is more effective against NCI-H460. Both extracts exhibited toxicity against non-tumoral cell lines, denoting that these extracts have cytotoxicity. The in vitro anti inflammatory potential of extracts was evaluated by measuring NO inhibition and the most effective extract was the flower with an EC50 values of 41±1 μg/mL. These results indicate that stems and flowers of C. erecta might be a potential source of natural biomolecules for pharmaceutical and food applications.application/pdfpt_PTCommelina erecta as a source of natural bioactive compoundsCavichi, Lucas V.PersonalFernandes, ÂngelaDSpacehttp://dspace.org/items/6233e657-3885-40a3-885e-0d51cf4dbaadDSpacehttp://dspace.org/items/6233e657-3885-40a3-885e-0d51cf4dbaadFernandesÂngelaCiência IDhttps://www.ciencia-id.pt2015-0434-AE7CORCIDhttp://orcid.org0000-0002-0157-9873PersonalMandim, FilipaDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdMandimFilipaCiência IDhttps://www.ciencia-id.ptF318-9049-EB99ORCIDhttp://orcid.org0000-0002-6494-2326PersonalPinela, JoséDSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4DSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4PinelaJoséCiência IDhttps://www.ciencia-id.pt771C-2B43-B108ORCIDhttp://orcid.org0000-0001-7523-1637Researcher IDhttps://www.researcherid.comB-4466-2014Scopus Author IDhttps://www.scopus.com54392272800Canan, CristianePersonalAmaral, Joana S.DSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdDSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdAmaralJoana S.Ciência IDhttps://www.ciencia-id.pt5319-7DE8-BEDAORCIDhttp://orcid.org0000-0002-3648-7303PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-972-745-286-62022-01-20T16:11:49Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/24808http://purl.org/coar/access_right/c_abf2open accessCommelina erecta LErva-de-santa-luzia768018 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/4ca7f1c2-1b73-4193-803a-762c9382d3a3/downloadNatural Products Application |
| spellingShingle | Commelina erecta as a source of natural bioactive compounds Cavichi, Lucas V. Commelina erecta L Erva-de-santa-luzia |
| status | SINGLETON |
| subject.fl_str_mv | Commelina erecta L Erva-de-santa-luzia |
| title | Commelina erecta as a source of natural bioactive compounds |
| title_full | Commelina erecta as a source of natural bioactive compounds |
| title_fullStr | Commelina erecta as a source of natural bioactive compounds |
| title_full_unstemmed | Commelina erecta as a source of natural bioactive compounds |
| title_short | Commelina erecta as a source of natural bioactive compounds |
| title_sort | Commelina erecta as a source of natural bioactive compounds |
| topic | Commelina erecta L Erva-de-santa-luzia |
| topic_facet | Commelina erecta L Erva-de-santa-luzia |
| url | http://hdl.handle.net/10198/24808 |
| visible | 1 |