Publicação
Erythrocyte deformability in a hyperbolic microchannel
| Resumo: | Serious diseases such as diabetes and malaria are deeply related to microcirculation disorders caused by abnormal erythrocytes deformability [1]. Therefore, various studies on red blood cells (RBCs) deformation have been carried out but those studies rather focus on shear ow or extensional ow [2-6]. In this study, a relatively low aspect ratio (AR) hyperbolic microchannel was used in order to create an extensional ow combined with a shear ow, where AR= h w , h means the depth and w means the width of the microchannel. The objective of the study is to investigate the degree of RBC deformation throughout the microchannel at the centerline (y = 0). The blood samples were RBCs diluted with the Hank's Balanced Salt Solution (HBSS) with the hematocrit (Hct) level set to be 2%. A polydimethylsiloxane (PDMS) microchannel, having a hyperbolic contraction region followed by an abrupt expansion shape, was fabricated by a soft-lithography technique. The dimensions of the microchannel: width of the inlet (w), length of contraction region (l), width of the exit of contraction (wc) and depth (h) were 400 m, 580 m, 20 m and 14 m, respectively. Hence, the AR was 0:035. The experimental equipment consisted of an inverted microscope (Diaphot 300, Nikon), a high-speed camera (FASTCAM SA3, Photron) connected to a computer and a 1mL syringe (TERUMO R SYRING) controlled by a syringe pump (PHD ULTRA). The manufactured PDMS microchannel was placed on the stage of the microscope where the ow rate Q of the working uids was kept constant at 0:5 L=min. The owing RBCs were recorded by a high speed camera at a frame rate of 7500 frames/s and analyzed. The DI was obtained by DI= a b a + b where a refers to the major axis and b refers to the minor axis of the ellipse best tted to RBCs. High DI values in the contraction region were observed. It is possible to say that the RBCs highly elongate with both extensional and shear dominated ows achieved by a hyperbolic microchannel with a relatively low aspect ratio AR. |
|---|---|
| Autores principais: | Faustino, Vera |
| Outros Autores: | Yaginuma, Tomoko; Pinho, Diana; Calhelha, Ricardo C.; Ferreira, Isabel C.F.R.; Lima, Rui A. |
| Assunto: | Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851105251753984 |
|---|---|
| author | Faustino, Vera |
| author2 | Yaginuma, Tomoko Pinho, Diana Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Lima, Rui A. |
| author2_role | author author author author author |
| author_facet | Faustino, Vera Yaginuma, Tomoko Pinho, Diana Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Faustino, Vera\"},{\"Person.name\":\"Yaginuma, Tomoko\"},{\"Person.name\":\"Pinho, Diana\",\"Person.identifier.orcid\":\"0000-0002-3884-6496\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Lima, Rui A.\",\"Person.identifier.orcid\":\"0000-0003-3428-637X\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Faustino, Vera Yaginuma, Tomoko Pinho, Diana Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-05-14T11:12:44Z |
| datacite.date.embargoed.fl_str_mv | 2014-05-14T11:12:44Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| datacite.titles.title.fl_str_mv | Erythrocyte deformability in a hyperbolic microchannel |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Faustino, Vera Yaginuma, Tomoko Pinho, Diana Calhelha, Ricardo C. Ferreira, Isabel C.F.R. Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-05-14T11:12:44Z |
| dc.date.embargoed.fl_str_mv | 2014-05-14T11:12:44Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/9512 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| dc.title.fl_str_mv | Erythrocyte deformability in a hyperbolic microchannel |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Serious diseases such as diabetes and malaria are deeply related to microcirculation disorders caused by abnormal erythrocytes deformability [1]. Therefore, various studies on red blood cells (RBCs) deformation have been carried out but those studies rather focus on shear ow or extensional ow [2-6]. In this study, a relatively low aspect ratio (AR) hyperbolic microchannel was used in order to create an extensional ow combined with a shear ow, where AR= h w , h means the depth and w means the width of the microchannel. The objective of the study is to investigate the degree of RBC deformation throughout the microchannel at the centerline (y = 0). The blood samples were RBCs diluted with the Hank's Balanced Salt Solution (HBSS) with the hematocrit (Hct) level set to be 2%. A polydimethylsiloxane (PDMS) microchannel, having a hyperbolic contraction region followed by an abrupt expansion shape, was fabricated by a soft-lithography technique. The dimensions of the microchannel: width of the inlet (w), length of contraction region (l), width of the exit of contraction (wc) and depth (h) were 400 m, 580 m, 20 m and 14 m, respectively. Hence, the AR was 0:035. The experimental equipment consisted of an inverted microscope (Diaphot 300, Nikon), a high-speed camera (FASTCAM SA3, Photron) connected to a computer and a 1mL syringe (TERUMO R SYRING) controlled by a syringe pump (PHD ULTRA). The manufactured PDMS microchannel was placed on the stage of the microscope where the ow rate Q of the working uids was kept constant at 0:5 L=min. The owing RBCs were recorded by a high speed camera at a frame rate of 7500 frames/s and analyzed. The DI was obtained by DI= a b a + b where a refers to the major axis and b refers to the minor axis of the ellipse best tted to RBCs. High DI values in the contraction region were observed. It is possible to say that the RBCs highly elongate with both extensional and shear dominated ows achieved by a hyperbolic microchannel with a relatively low aspect ratio AR. |
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| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 5876-PPCDTI 5876-PPCDTI |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/9512 |
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| institution | Instituto Politécnico de Bragança |
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| person_str_mv | Faustino, Vera Yaginuma, Tomoko Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Lima, Rui A. Lima, Rui A. https://www.ciencia-id.pt/EE12-C3FB-349D EE12-C3FB-349D http://orcid.org/0000-0003-3428-637X 0000-0003-3428-637X |
| publishDate | 2013 |
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| spelling | engporSerious diseases such as diabetes and malaria are deeply related to microcirculation disorders caused by abnormal erythrocytes deformability [1]. Therefore, various studies on red blood cells (RBCs) deformation have been carried out but those studies rather focus on shear ow or extensional ow [2-6]. In this study, a relatively low aspect ratio (AR) hyperbolic microchannel was used in order to create an extensional ow combined with a shear ow, where AR= h w , h means the depth and w means the width of the microchannel. The objective of the study is to investigate the degree of RBC deformation throughout the microchannel at the centerline (y = 0). The blood samples were RBCs diluted with the Hank's Balanced Salt Solution (HBSS) with the hematocrit (Hct) level set to be 2%. A polydimethylsiloxane (PDMS) microchannel, having a hyperbolic contraction region followed by an abrupt expansion shape, was fabricated by a soft-lithography technique. The dimensions of the microchannel: width of the inlet (w), length of contraction region (l), width of the exit of contraction (wc) and depth (h) were 400 m, 580 m, 20 m and 14 m, respectively. Hence, the AR was 0:035. The experimental equipment consisted of an inverted microscope (Diaphot 300, Nikon), a high-speed camera (FASTCAM SA3, Photron) connected to a computer and a 1mL syringe (TERUMO R SYRING) controlled by a syringe pump (PHD ULTRA). The manufactured PDMS microchannel was placed on the stage of the microscope where the ow rate Q of the working uids was kept constant at 0:5 L=min. The owing RBCs were recorded by a high speed camera at a frame rate of 7500 frames/s and analyzed. The DI was obtained by DI= a b a + b where a refers to the major axis and b refers to the minor axis of the ellipse best tted to RBCs. High DI values in the contraction region were observed. It is possible to say that the RBCs highly elongate with both extensional and shear dominated ows achieved by a hyperbolic microchannel with a relatively low aspect ratio AR.application/pdfporErythrocyte deformability in a hyperbolic microchannelFaustino, VeraYaginuma, TomokoPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalLima, Rui A.DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62LimaRui A.Ciência IDhttps://www.ciencia-id.ptEE12-C3FB-349DORCIDhttp://orcid.org0000-0003-3428-637XResearcher IDhttps://www.researcherid.comH-5157-2016Scopus Author IDhttps://www.scopus.com18437397800HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2014-05-14T11:12:44Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/9512http://purl.org/coar/access_right/c_abf2open accessErythrocytesRed blood cellDeformabilityDeformation indexExtensional flowShear flow106293 bytesFundação para a Ciência e a TecnologiaHemo-Networks - Experimental and computational investigations of the factors influencing blood flow in microvascular networks.5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/acd58745-6191-4944-b2c6-32752fe5343d/downloadPortugal Workshop on Modeling and Simulation of Physiological SystemsLisboa |
| spellingShingle | Erythrocyte deformability in a hyperbolic microchannel Faustino, Vera Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| subject.fl_str_mv | Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| title | Erythrocyte deformability in a hyperbolic microchannel |
| title_full | Erythrocyte deformability in a hyperbolic microchannel |
| title_fullStr | Erythrocyte deformability in a hyperbolic microchannel |
| title_full_unstemmed | Erythrocyte deformability in a hyperbolic microchannel |
| title_short | Erythrocyte deformability in a hyperbolic microchannel |
| title_sort | Erythrocyte deformability in a hyperbolic microchannel |
| topic | Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| topic_facet | Erythrocytes Red blood cell Deformability Deformation index Extensional flow Shear flow |
| url | http://hdl.handle.net/10198/9512 |
| visible | 1 |