Publicação
Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice
| Resumo: | Obesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life. |
|---|---|
| Autores principais: | Rocha, Paola dos Santos |
| Outros Autores: | Orué, Sarah Lam; Leite, Daniel Ferreira; Espindola, Priscilla Pereira de Toledo; Cassemiro, Nadla Soares; Silva, Denise Brentan; Carollo, Carlos Alexandre; Nunes-Souza, Valéria; Rabelo, Luiza Antas; Campos, Jaqueline Ferreira; Estevinho, Leticia M.; Santos, Edson Lucas dos |
| Assunto: | Antioxidants Blood Flavonoids Quality Control |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851246151008256 |
|---|---|
| author | Rocha, Paola dos Santos |
| author2 | Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
| author2_role | author author author author author author author author author author author |
| author_facet | Rocha, Paola dos Santos Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Rocha, Paola dos Santos\"},{\"Person.name\":\"Orué, Sarah Lam\"},{\"Person.name\":\"Leite, Daniel Ferreira\"},{\"Person.name\":\"Espindola, Priscilla Pereira de Toledo\"},{\"Person.name\":\"Cassemiro, Nadla Soares\"},{\"Person.name\":\"Silva, Denise Brentan\"},{\"Person.name\":\"Carollo, Carlos Alexandre\"},{\"Person.name\":\"Nunes-Souza, Valéria\"},{\"Person.name\":\"Rabelo, Luiza Antas\"},{\"Person.name\":\"Campos, Jaqueline Ferreira\"},{\"Person.name\":\"Estevinho, Leticia M.\",\"Person.identifier.orcid\":\"0000-0002-9249-1948\"},{\"Person.name\":\"Santos, Edson Lucas dos\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Rocha, Paola dos Santos Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-11-04T10:38:05Z |
| datacite.date.embargoed.fl_str_mv | 2022-11-04T10:38:05Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Antioxidants Blood Flavonoids Quality Control |
| datacite.titles.title.fl_str_mv | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Rocha, Paola dos Santos Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Santos, Edson Lucas dos |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-11-04T10:38:05Z |
| dc.date.embargoed.fl_str_mv | 2022-11-04T10:38:05Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/26067 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Hindawi |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Antioxidants Blood Flavonoids Quality Control |
| dc.title.fl_str_mv | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Obesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/073fac6c-9cfa-4e03-be33-f7d8fa899055/download |
| id | ipb_69511ed4e4de8ea25e394f537a00ea24 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/26067 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/26067 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Rocha, Paola dos Santos Orué, Sarah Lam Leite, Daniel Ferreira Espindola, Priscilla Pereira de Toledo Cassemiro, Nadla Soares Silva, Denise Brentan Carollo, Carlos Alexandre Nunes-Souza, Valéria Rabelo, Luiza Antas Campos, Jaqueline Ferreira Estevinho, Leticia M. Estevinho, Leticia M. https://www.ciencia-id.pt/BA14-09D6-A406 BA14-09D6-A406 http://orcid.org/0000-0002-9249-1948 0000-0002-9249-1948 Santos, Edson Lucas dos |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Hindawi |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engHindawipt_PTObesity is an epidemic disease worldwide, associated with oxidative stress and the development of several other diseases. Bauhinia rufa (Bong.) Steud. is a native Brazilian Cerrado medicinal plant popularly used for the treatment of obesity. In this context, we investigated the chemical composition of the methanolic extract of B. rufa leaves (MEBr) and evaluated the antioxidant activity and its impact on the prevention and treatment of obesity in mice fed a high-fat diet (HFD 60%). Additionally, the acute oral toxicity of MEBr was evaluated. In MEBr, 17 glycosylated compounds were identified, including myricetin, quercetin, kaempferol, coumaroyl, cyanoglucoside, and megastigmane. In vitro, MEBr showed antioxidant activity in different methods: DPPH•, ABTS•+, FRAP, iron-reducing power, inhibition of β-carotene bleaching, and inhibition of DNA fragmentation. In human erythrocytes, MEBr increased the activities of antioxidant enzymes, superoxide dismutase, and catalase. Under oxidative stress, MEBr reduced oxidative hemolysis, and the malondialdehyde (MDA) levels generated in erythrocytes. Mice treated acutely with MEBr (2000 mg/kg) showed no signs of toxicity. During 90 days, the mice received water or MEBr simultaneously with HFD for induction of obesity. At this stage, MEBr was able to reduce the gain of subcutaneous white adipose tissue (WAT) and prevent the increase of MDA in the heart and brain. After 180 days of HFD for obesity induction, mice that received MEBr simultaneously with HFD (HFD-MEBr) in the last 60 days of treatment (120-180 days) showed a reduction of retroperitoneal and mesenteric WAT deposits and MDA levels in the heart, liver, kidney, and brain, compared to the HFD-Control group. These effects of MEBr were similar to mice treated with sibutramine (HFD-Sibutramine, 2 mg/kg). Combined, the results show that compounds from the leaves of B. rufa affect controlling oxidative stress and actions in the prevention and treatment of obesity. Thus, associated oxidative stress reduction and body composition modulation, in obese people, can contribute to the prevention of obesity-related comorbidities and improve quality of life.application/pdfpt_PTBeneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 miceRocha, Paola dos SantosOrué, Sarah LamLeite, Daniel FerreiraEspindola, Priscilla Pereira de ToledoCassemiro, Nadla SoaresSilva, Denise BrentanCarollo, Carlos AlexandreNunes-Souza, ValériaRabelo, Luiza AntasCampos, Jaqueline FerreiraPersonalEstevinho, Leticia M.DSpacehttp://dspace.org/items/4a1d5ba2-1854-4ca5-89a4-73f35e964df9DSpacehttp://dspace.org/items/4a1d5ba2-1854-4ca5-89a4-73f35e964df9EstevinhoLetícia M.Ciência IDhttps://www.ciencia-id.ptBA14-09D6-A406ORCIDhttp://orcid.org0000-0002-9249-1948Scopus Author IDhttps://www.scopus.com6506577664Scopus Author IDhttps://www.scopus.com57211945650Santos, Edson Lucas dosHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf194209002022-11-04T10:38:05Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/26067http://purl.org/coar/access_right/c_abf2open accessAntioxidantsBloodFlavonoidsQuality Control900539 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/073fac6c-9cfa-4e03-be33-f7d8fa899055/downloadOxidative Medicine and Cellular Longevity |
| spellingShingle | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice Rocha, Paola dos Santos Antioxidants Blood Flavonoids Quality Control |
| subject.fl_str_mv | Antioxidants Blood Flavonoids Quality Control |
| title | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| title_full | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| title_fullStr | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| title_full_unstemmed | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| title_short | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| title_sort | Beneficial effects of bauhinia rufa leaves on oxidative stress, prevention, and treatment of obesity in high-fat diet-fed C57BL/6 mice |
| topic | Antioxidants Blood Flavonoids Quality Control |
| topic_facet | Antioxidants Blood Flavonoids Quality Control |
| url | http://hdl.handle.net/10198/26067 |
| visible | 1 |