Publicação
Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI
| Resumo: | Beverage industry produces a large and diverse range of soft drinks, beverages containing flavourings and/or fruit juices (sodas and fruit juices), of which t he quality and safety must be monitored to protect and satisfy customers. From the raw ingredients to the final product, quality control is needed to ensure product safety, quality, labelling, regulatory compliance and consistency. The development of analytical techniques for simultaneous analysis of different compounds essential to control the product quality, as an alternative to several independent traditional reference methods, is of major importance. Therefore, the present work reports the application of size exclusion chromatography (SEC), which allows carrying out analysis free of organic solvents, using two detectors coupled in series - Ultraviolet (UV) and Refractive Index (RI) - for the simultaneous analysis of acidifiers (citric, tartaric, lactic, acetic, malic and ascorbic acids, by UV), and sweeteners (sucrose, glucose and fructose by RI), in commercial non-alcoholic beverages with different levels of added fruit juice. Ascorbic acid is used as a stabilizer in the soft drinks, improving the beverage shelf-life stability due to its antioxidant properties. The results showed that the simultaneous calibrations for acid compounds (UV) and for glucose (RI) were straight-forward. On the other hand, for sucrose and fructose simultaneous analysis, the calibrations (RI) were more complex since the predictive models established had to take into account malic and tartaric acids interferences, regardless the good resolution between the peaks of sucrose and fructose. Finally, the results for sample analysis showed that all the sugars evaluated were present in the juice drinks as well as the citric, tartaric, malic and ascorbic acids. In all samples, lactic and acetic acids were not detected. |
|---|---|
| Autores principais: | Sequeira, Cédric Basílio |
| Outros Autores: | Dias, L.G.; Morais, Jorge Sá; Veloso, Ana C.A.; Machado, Adélio A.M.; Peres, António M. |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173317670076416 |
|---|---|
| author | Sequeira, Cédric Basílio |
| author2 | Dias, L.G. Morais, Jorge Sá Veloso, Ana C.A. Machado, Adélio A.M. Peres, António M. |
| author2_role | author author author author author |
| author_facet | Sequeira, Cédric Basílio Dias, L.G. Morais, Jorge Sá Veloso, Ana C.A. Machado, Adélio A.M. Peres, António M. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Sequeira, Cédric Basílio\"},{\"Person.name\":\"Dias, L.G.\",\"Person.identifier.orcid\":\"0000-0002-1210-4259\"},{\"Person.name\":\"Morais, Jorge Sá\"},{\"Person.name\":\"Veloso, Ana C.A.\"},{\"Person.name\":\"Machado, Adélio A.M.\"},{\"Person.name\":\"Peres, António M.\",\"Person.identifier.orcid\":\"0000-0001-6595-9165\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Sequeira, Cédric Basílio Dias, L.G. Morais, Jorge Sá Veloso, Ana C.A. Machado, Adélio A.M. Peres, António M. |
| datacite.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-10-16T14:06:50Z |
| datacite.date.embargoed.fl_str_mv | 2014-10-16T14:06:50Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Sequeira, Cédric Basílio Dias, L.G. Morais, Jorge Sá Veloso, Ana C.A. Machado, Adélio A.M. Peres, António M. |
| dc.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-10-16T14:06:50Z |
| dc.date.embargoed.fl_str_mv | 2014-10-16T14:06:50Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/10872 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Beverage industry produces a large and diverse range of soft drinks, beverages containing flavourings and/or fruit juices (sodas and fruit juices), of which t he quality and safety must be monitored to protect and satisfy customers. From the raw ingredients to the final product, quality control is needed to ensure product safety, quality, labelling, regulatory compliance and consistency. The development of analytical techniques for simultaneous analysis of different compounds essential to control the product quality, as an alternative to several independent traditional reference methods, is of major importance. Therefore, the present work reports the application of size exclusion chromatography (SEC), which allows carrying out analysis free of organic solvents, using two detectors coupled in series - Ultraviolet (UV) and Refractive Index (RI) - for the simultaneous analysis of acidifiers (citric, tartaric, lactic, acetic, malic and ascorbic acids, by UV), and sweeteners (sucrose, glucose and fructose by RI), in commercial non-alcoholic beverages with different levels of added fruit juice. Ascorbic acid is used as a stabilizer in the soft drinks, improving the beverage shelf-life stability due to its antioxidant properties. The results showed that the simultaneous calibrations for acid compounds (UV) and for glucose (RI) were straight-forward. On the other hand, for sucrose and fructose simultaneous analysis, the calibrations (RI) were more complex since the predictive models established had to take into account malic and tartaric acids interferences, regardless the good resolution between the peaks of sucrose and fructose. Finally, the results for sample analysis showed that all the sugars evaluated were present in the juice drinks as well as the citric, tartaric, malic and ascorbic acids. In all samples, lactic and acetic acids were not detected. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/10872 |
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| institution | Instituto Politécnico de Bragança |
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| person_str_mv | Sequeira, Cédric Basílio Dias, L.G. Dias, L.G. https://www.ciencia-id.pt/2F11-9092-FAAF 2F11-9092-FAAF http://orcid.org/0000-0002-1210-4259 0000-0002-1210-4259 Morais, Jorge Sá Morais, Jorge Sá https://www.ciencia-id.pt/6E11-BDED-4CB3 6E11-BDED-4CB3 Veloso, Ana C.A. Machado, Adélio A.M. Peres, António M. Peres, António M. https://www.ciencia-id.pt/CF16-5443-F420 CF16-5443-F420 http://orcid.org/0000-0001-6595-9165 0000-0001-6595-9165 |
| publishDate | 2012 |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engporBeverage industry produces a large and diverse range of soft drinks, beverages containing flavourings and/or fruit juices (sodas and fruit juices), of which t he quality and safety must be monitored to protect and satisfy customers. From the raw ingredients to the final product, quality control is needed to ensure product safety, quality, labelling, regulatory compliance and consistency. The development of analytical techniques for simultaneous analysis of different compounds essential to control the product quality, as an alternative to several independent traditional reference methods, is of major importance. Therefore, the present work reports the application of size exclusion chromatography (SEC), which allows carrying out analysis free of organic solvents, using two detectors coupled in series - Ultraviolet (UV) and Refractive Index (RI) - for the simultaneous analysis of acidifiers (citric, tartaric, lactic, acetic, malic and ascorbic acids, by UV), and sweeteners (sucrose, glucose and fructose by RI), in commercial non-alcoholic beverages with different levels of added fruit juice. Ascorbic acid is used as a stabilizer in the soft drinks, improving the beverage shelf-life stability due to its antioxidant properties. The results showed that the simultaneous calibrations for acid compounds (UV) and for glucose (RI) were straight-forward. On the other hand, for sucrose and fructose simultaneous analysis, the calibrations (RI) were more complex since the predictive models established had to take into account malic and tartaric acids interferences, regardless the good resolution between the peaks of sucrose and fructose. Finally, the results for sample analysis showed that all the sugars evaluated were present in the juice drinks as well as the citric, tartaric, malic and ascorbic acids. In all samples, lactic and acetic acids were not detected.application/pdfporAnalysis of acids and sugars in fruit-based drinks by SEC-UV-RISequeira, Cédric BasílioPersonalDias, L.G.DSpacehttp://dspace.org/items/eac8c166-4056-4ed0-8d8d-7ecb2c4481a5DSpacehttp://dspace.org/items/eac8c166-4056-4ed0-8d8d-7ecb2c4481a5DiasLuís G.Ciência IDhttps://www.ciencia-id.pt2F11-9092-FAAFORCIDhttp://orcid.org0000-0002-1210-4259Scopus Author IDhttps://www.scopus.com23569169900PersonalMorais, Jorge SáDSpacehttp://dspace.org/items/c2ed0055-91c1-4bf5-acb3-d9a99296d9a2DSpacehttp://dspace.org/items/c2ed0055-91c1-4bf5-acb3-d9a99296d9a2MoraisJorge SáCiência IDhttps://www.ciencia-id.pt6E11-BDED-4CB3Veloso, Ana C.A.Machado, Adélio A.M.PersonalPeres, António M.DSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbDSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbPeresAntónio M.Ciência IDhttps://www.ciencia-id.ptCF16-5443-F420ORCIDhttp://orcid.org0000-0001-6595-9165Researcher IDhttps://www.researcherid.comI-8470-2012Scopus Author IDhttps://www.scopus.com7102331969HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2014-10-16T14:06:50Z20122012-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/10872http://purl.org/coar/access_right/c_abf2open access1275169 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/04fbf1f0-dfcd-45c6-9f07-7142facf1eb1/download7º Encontro Nacional de CromatografiaUniversidade do Porto |
| spellingShingle | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI Sequeira, Cédric Basílio |
| status | SINGLETON |
| title | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| title_full | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| title_fullStr | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| title_full_unstemmed | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| title_short | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| title_sort | Analysis of acids and sugars in fruit-based drinks by SEC-UV-RI |
| url | http://hdl.handle.net/10198/10872 |
| visible | 1 |