Publicação
Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves
| Resumo: | The ectomycorrhizal (ECM) symbiosis that develops between the roots of host trees and the soil ECM fungi is an important factor towards the survival, health and growth of these trees, as it stimulates their water and nutrient uptake. Ectomycorrhizal colonization can result in the deposition of phenolic compounds in peripheral cortex cells and a similar answer can be recognized as one way of plant defense against pathogenic infections. The aim of the present work was the evaluation of antioxidant potential of the ectomycorrhizal fungi, Paxillus involutus and Pisolithus arhizus, in presence and absence of the symbiont – Pinus pinaster –, in response to the symbiotic association, under different contact periods (45 days and 48 h). Phenolic contents in mycelia, culture media and plant leaves, stems and roots were determined by Folin-Ciocalteu assay, and their antioxidant properties were evaluated by three in vitro assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation inhibition through B-carotene bleaching inhibition. Comparing the response of the symbionts to the host species, Pinus pinaster benefited most with the association, because it generally decreases the phenolics content and the antioxidant activity values in the presence of both ectomycorrhizal fungi along time. Pisolithus arhizus also decreased, in general, its phenolics levels and antioxidant properties, alongside with Pinus pinaster, unlike Paxillus involutus that did not have the same response as it increases its content of phenolics and some of its values of antioxidant activity. These results can be considered as an hypothetical signal of a symbiotic differential compatibility of mycorrhizal fungi for a host. |
|---|---|
| Autores principais: | Reis, Filipa S. |
| Outros Autores: | Ferreira, Isabel C.F.R.; Martins, Anabela |
| Assunto: | Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172952727879680 |
|---|---|
| author | Reis, Filipa S. |
| author2 | Ferreira, Isabel C.F.R. Martins, Anabela |
| author2_role | author author |
| author_facet | Reis, Filipa S. Ferreira, Isabel C.F.R. Martins, Anabela |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Reis, Filipa S.\",\"Person.identifier.orcid\":\"0000-0002-9159-0530\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Martins, Anabela\",\"Person.identifier.orcid\":\"0000-0001-6218-4413\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Reis, Filipa S. Ferreira, Isabel C.F.R. Martins, Anabela |
| datacite.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-10-25T10:36:20Z |
| datacite.date.embargoed.fl_str_mv | 2011-10-25T10:36:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| datacite.titles.title.fl_str_mv | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Reis, Filipa S. Ferreira, Isabel C.F.R. Martins, Anabela |
| dc.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-10-25T10:36:20Z |
| dc.date.embargoed.fl_str_mv | 2011-10-25T10:36:20Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/6234 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| dc.title.fl_str_mv | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The ectomycorrhizal (ECM) symbiosis that develops between the roots of host trees and the soil ECM fungi is an important factor towards the survival, health and growth of these trees, as it stimulates their water and nutrient uptake. Ectomycorrhizal colonization can result in the deposition of phenolic compounds in peripheral cortex cells and a similar answer can be recognized as one way of plant defense against pathogenic infections. The aim of the present work was the evaluation of antioxidant potential of the ectomycorrhizal fungi, Paxillus involutus and Pisolithus arhizus, in presence and absence of the symbiont – Pinus pinaster –, in response to the symbiotic association, under different contact periods (45 days and 48 h). Phenolic contents in mycelia, culture media and plant leaves, stems and roots were determined by Folin-Ciocalteu assay, and their antioxidant properties were evaluated by three in vitro assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation inhibition through B-carotene bleaching inhibition. Comparing the response of the symbionts to the host species, Pinus pinaster benefited most with the association, because it generally decreases the phenolics content and the antioxidant activity values in the presence of both ectomycorrhizal fungi along time. Pisolithus arhizus also decreased, in general, its phenolics levels and antioxidant properties, alongside with Pinus pinaster, unlike Paxillus involutus that did not have the same response as it increases its content of phenolics and some of its values of antioxidant activity. These results can be considered as an hypothetical signal of a symbiotic differential compatibility of mycorrhizal fungi for a host. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/1d676269-8131-4598-9872-9157dda2869b/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 5876-PPCDTI |
| id | ipb_879eb913cf4ddcbdcf8be1b63d8ff2d1 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/6234 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/6234 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Reis, Filipa S. Reis, Filipa S. https://www.ciencia-id.pt/391F-AFE1-64C7 391F-AFE1-64C7 http://orcid.org/0000-0002-9159-0530 0000-0002-9159-0530 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Martins, Anabela Martins, Anabela https://www.ciencia-id.pt/7B18-A810-6B93 7B18-A810-6B93 http://orcid.org/0000-0001-6218-4413 0000-0001-6218-4413 |
| publishDate | 2012 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engElsevierporThe ectomycorrhizal (ECM) symbiosis that develops between the roots of host trees and the soil ECM fungi is an important factor towards the survival, health and growth of these trees, as it stimulates their water and nutrient uptake. Ectomycorrhizal colonization can result in the deposition of phenolic compounds in peripheral cortex cells and a similar answer can be recognized as one way of plant defense against pathogenic infections. The aim of the present work was the evaluation of antioxidant potential of the ectomycorrhizal fungi, Paxillus involutus and Pisolithus arhizus, in presence and absence of the symbiont – Pinus pinaster –, in response to the symbiotic association, under different contact periods (45 days and 48 h). Phenolic contents in mycelia, culture media and plant leaves, stems and roots were determined by Folin-Ciocalteu assay, and their antioxidant properties were evaluated by three in vitro assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation inhibition through B-carotene bleaching inhibition. Comparing the response of the symbionts to the host species, Pinus pinaster benefited most with the association, because it generally decreases the phenolics content and the antioxidant activity values in the presence of both ectomycorrhizal fungi along time. Pisolithus arhizus also decreased, in general, its phenolics levels and antioxidant properties, alongside with Pinus pinaster, unlike Paxillus involutus that did not have the same response as it increases its content of phenolics and some of its values of antioxidant activity. These results can be considered as an hypothetical signal of a symbiotic differential compatibility of mycorrhizal fungi for a host.application/pdfapplication/pdfporEffect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leavesPersonalReis, Filipa S.DSpacehttp://dspace.org/items/b8d384ae-2134-4735-93a6-0d2febbf9220DSpacehttp://dspace.org/items/b8d384ae-2134-4735-93a6-0d2febbf9220ReisFilipa S.Ciência IDhttps://www.ciencia-id.pt391F-AFE1-64C7ORCIDhttp://orcid.org0000-0002-9159-0530Researcher IDhttps://www.researcherid.comI-2093-2013Scopus Author IDhttps://www.scopus.com36982144400PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalMartins, AnabelaDSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581DSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581MartinsAnabelaCiência IDhttps://www.ciencia-id.pt7B18-A810-6B93ORCIDhttp://orcid.org0000-0001-6218-4413Researcher IDhttps://www.researcherid.comG-5488-2013Scopus Author IDhttps://www.scopus.com7203013518HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0926-6690DOIIsPartOf10.1016/j.indcrop.2011.06.0382011-10-25T10:36:20Z20122012-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/6234http://purl.org/coar/access_right/c_16ecrestricted accessEctomycorrhizal fungiPinus pinasterSymbiosisContact periodAntioxidant potential655841 bytes299199 bytesFundação para a Ciência e a TecnologiaPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/1d676269-8131-4598-9872-9157dda2869b/downloadhttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/3f0780b7-f94e-4c0b-8669-76cad1dd588b/downloadIndustrial Crops and Products35211216 |
| spellingShingle | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves Reis, Filipa S. Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| status | SINGLETON |
| subject.fl_str_mv | Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| title | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| title_full | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| title_fullStr | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| title_full_unstemmed | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| title_short | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| title_sort | Effect of the mycorrhizal symbiosis time in the antioxidant activity of fungi and Pinus pinaster roots, stems and leaves |
| topic | Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| topic_facet | Ectomycorrhizal fungi Pinus pinaster Symbiosis Contact period Antioxidant potential |
| url | http://hdl.handle.net/10198/6234 |
| visible | 1 |