Publicação
Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry
| Resumo: | A simple and rapid microwave-assisted alkaline digestion procedure was developed in combination with high performance liquid chromatography-ultraviolet post-column oxidation-cold vapour atomic fluorescence spectrometric detection for methylmercury determination in biological tissues. Since the stability of methylmercury in methanolic potassium hydroxide solution under microwave irradiation was verified, the microwave-assisted extraction procedure was optimized in terms of quantitative recovery of methylmercury and minimum time required. The alkaline extracts were subjected to clean-up steps with dichloromethane and hydrochloric acid in order to reduce matrix interferences in methylmercury determination. The effects of matrix interferences were checked by comparison of the slopes corresponding to calibration and standard addition curves. The accuracy of the method was evaluated by the analysis of two biological certified reference materials, NRC TORT-2 and BCR 463. The results obtained by the proposed method were in good agreement with the certified values of methylmercury concentration in both materials. The detection limit was 10 mug kg(-1) and the relative standard deviation was < 8% for methylmercury concentrations ranging from 0.15 to 3.0 mg kg(-1). |
|---|---|
| Autores principais: | Ramalhosa, Elsa |
| Outros Autores: | Río-Segade, Susana; Pereira, Eduarda; Vale, Carlos; Duarte, Armando |
| Assunto: | Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| Ano: | 2001 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172978057281536 |
|---|---|
| author | Ramalhosa, Elsa |
| author2 | Río-Segade, Susana Pereira, Eduarda Vale, Carlos Duarte, Armando |
| author2_role | author author author author |
| author_facet | Ramalhosa, Elsa Río-Segade, Susana Pereira, Eduarda Vale, Carlos Duarte, Armando |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ramalhosa, Elsa\",\"Person.identifier.orcid\":\"0000-0003-2503-9705\"},{\"Person.name\":\"Río-Segade, Susana\"},{\"Person.name\":\"Pereira, Eduarda\"},{\"Person.name\":\"Vale, Carlos\"},{\"Person.name\":\"Duarte, Armando\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Ramalhosa, Elsa Río-Segade, Susana Pereira, Eduarda Vale, Carlos Duarte, Armando |
| datacite.date.Accepted.fl_str_mv | 2001-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-04-05T09:44:40Z |
| datacite.date.embargoed.fl_str_mv | 2011-04-05T09:44:40Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| datacite.titles.title.fl_str_mv | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Ramalhosa, Elsa Río-Segade, Susana Pereira, Eduarda Vale, Carlos Duarte, Armando |
| dc.date.Accepted.fl_str_mv | 2001-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-04-05T09:44:40Z |
| dc.date.embargoed.fl_str_mv | 2011-04-05T09:44:40Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/3950 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | The Royal Society of Chemistry |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| dc.title.fl_str_mv | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A simple and rapid microwave-assisted alkaline digestion procedure was developed in combination with high performance liquid chromatography-ultraviolet post-column oxidation-cold vapour atomic fluorescence spectrometric detection for methylmercury determination in biological tissues. Since the stability of methylmercury in methanolic potassium hydroxide solution under microwave irradiation was verified, the microwave-assisted extraction procedure was optimized in terms of quantitative recovery of methylmercury and minimum time required. The alkaline extracts were subjected to clean-up steps with dichloromethane and hydrochloric acid in order to reduce matrix interferences in methylmercury determination. The effects of matrix interferences were checked by comparison of the slopes corresponding to calibration and standard addition curves. The accuracy of the method was evaluated by the analysis of two biological certified reference materials, NRC TORT-2 and BCR 463. The results obtained by the proposed method were in good agreement with the certified values of methylmercury concentration in both materials. The detection limit was 10 mug kg(-1) and the relative standard deviation was < 8% for methylmercury concentrations ranging from 0.15 to 3.0 mg kg(-1). |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/20a5c9d7-9dc2-486c-bd5b-a2df763beb66/download |
| id | ipb_8b3d1c43bbf67b60eaa77eb2330eabbf |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/3950 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/3950 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Ramalhosa, Elsa Ramalhosa, Elsa https://www.ciencia-id.pt/1A1D-FC05-A05D 1A1D-FC05-A05D http://orcid.org/0000-0003-2503-9705 0000-0003-2503-9705 Río-Segade, Susana Pereira, Eduarda Vale, Carlos Duarte, Armando |
| publishDate | 2001 |
| publisher.none.fl_str_mv | The Royal Society of Chemistry |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engThe Royal Society of ChemistryporA simple and rapid microwave-assisted alkaline digestion procedure was developed in combination with high performance liquid chromatography-ultraviolet post-column oxidation-cold vapour atomic fluorescence spectrometric detection for methylmercury determination in biological tissues. Since the stability of methylmercury in methanolic potassium hydroxide solution under microwave irradiation was verified, the microwave-assisted extraction procedure was optimized in terms of quantitative recovery of methylmercury and minimum time required. The alkaline extracts were subjected to clean-up steps with dichloromethane and hydrochloric acid in order to reduce matrix interferences in methylmercury determination. The effects of matrix interferences were checked by comparison of the slopes corresponding to calibration and standard addition curves. The accuracy of the method was evaluated by the analysis of two biological certified reference materials, NRC TORT-2 and BCR 463. The results obtained by the proposed method were in good agreement with the certified values of methylmercury concentration in both materials. The detection limit was 10 mug kg(-1) and the relative standard deviation was < 8% for methylmercury concentrations ranging from 0.15 to 3.0 mg kg(-1).application/pdfMicrowave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometryPersonalRamalhosa, ElsaDSpacehttp://dspace.org/items/d74be6c0-1703-4f77-bd81-e289ec981ee3DSpacehttp://dspace.org/items/d74be6c0-1703-4f77-bd81-e289ec981ee3RamalhosaElsaCiência IDhttps://www.ciencia-id.pt1A1D-FC05-A05DORCIDhttp://orcid.org0000-0003-2503-9705Scopus Author IDhttps://www.scopus.com6602978189Río-Segade, SusanaPereira, EduardaVale, CarlosDuarte, ArmandoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.1039/b104041n2011-04-05T09:44:40Z20012001-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/3950http://purl.org/coar/access_right/c_abf2open accessMethylmercuryMicrowave treatmentBiological samplesHPLC-CV-AFS183365 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/20a5c9d7-9dc2-486c-bd5b-a2df763beb66/downloadAnalyst15831587 |
| spellingShingle | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry Ramalhosa, Elsa Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| status | SINGLETON |
| subject.fl_str_mv | Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| title | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| title_full | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| title_fullStr | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| title_full_unstemmed | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| title_short | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| title_sort | Microwave treatment of biological samples for methylmercury determination by high performance liquid chromatography-cold vapour atomic fluorescence spectrometry |
| topic | Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| topic_facet | Methylmercury Microwave treatment Biological samples HPLC-CV-AFS |
| url | http://hdl.handle.net/10198/3950 |
| visible | 1 |