Publicação
Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus
| Resumo: | Mushrooms are a source of compounds with promising antitumour activity [1]. We have been working on the identification of wild mushrooms with promising antitumour activity and a Clitocybe alexandri extract which induces cell cycle arrest and apoptosis in a lung cancer cell line has been previously identified by part of the team [2]. The objective of this work was to continue the identification of mushrooms from the Northeast of Portugal with tumour cell growth inhibitory potential. Thirty six wild edible mushrooms were collected and taxonomically identified. Various extracts were prepared and screened for growth inhibitory potential with the SRB assay, in four human tumour cell lines: MCF-7 (breast), NCI-H460 (NSCLC), AGS (gastric) and HCT-15 (colon). These screens allowed the identification of two Suillus species whose methanolic extracts presented potent activity: S. collinitus and S. luteus. The S. collinitus extract was more potent in the MCF-7 cells (GI50=25.2±0.16 µg/ml), causing G1 cell cycle arrest and increasing apoptosis. An increase in p53 and p21 was verified, suggesting that the effect was p53-mediated [3]. The S. luteus extract was slightly more potent in the HCT-15 cells (with mutant p53, GI50=17.8 ± 1.6 µg/ml) than in the other cell lines tested, indicating that its effect was not p53-dependent. In fact, it increased the levels of p53 but the alterations in proteins transactivated by p53 (e.g. Bax) were not consistent with a p53-mediated effect. Growth inhibition in the HCT-15 cells was mostly due to inhibition of cell proliferation and cell cycle arrest in G1, rather than induction of cell death. Finally, this extract had no effect in primary cultures of porcine hepatocytes (GI50>400 µg/ml). In conclusion, these two Suillus species present promising tumour cell growth inhibitory effect and the S. luteus extract may be particularly interesting considering that most tumours present mutant p53. |
|---|---|
| Autores principais: | Vaz, Josiana A. |
| Outros Autores: | Santos, Tiago; Tavares, Catarina; Calhelha, Ricardo C.; Martins, Anabela; Almeida, Gabriela M.; Ferreira, Isabel C.F.R.; Vasconcelos, M. Helena |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172950807937024 |
|---|---|
| author | Vaz, Josiana A. |
| author2 | Santos, Tiago Tavares, Catarina Calhelha, Ricardo C. Martins, Anabela Almeida, Gabriela M. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
| author2_role | author author author author author author author |
| author_facet | Vaz, Josiana A. Santos, Tiago Tavares, Catarina Calhelha, Ricardo C. Martins, Anabela Almeida, Gabriela M. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Vaz, Josiana A.\",\"Person.identifier.orcid\":\"0000-0002-6989-1169\"},{\"Person.name\":\"Santos, Tiago\"},{\"Person.name\":\"Tavares, Catarina\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Martins, Anabela\",\"Person.identifier.orcid\":\"0000-0001-6218-4413\"},{\"Person.name\":\"Almeida, Gabriela M.\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Vasconcelos, M. Helena\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Vaz, Josiana A. Santos, Tiago Tavares, Catarina Calhelha, Ricardo C. Martins, Anabela Almeida, Gabriela M. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
| datacite.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2013-02-27T15:24:04Z |
| datacite.date.embargoed.fl_str_mv | 2013-02-27T15:24:04Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Vaz, Josiana A. Santos, Tiago Tavares, Catarina Calhelha, Ricardo C. Martins, Anabela Almeida, Gabriela M. Ferreira, Isabel C.F.R. Vasconcelos, M. Helena |
| dc.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2013-02-27T15:24:04Z |
| dc.date.embargoed.fl_str_mv | 2013-02-27T15:24:04Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/8191 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Mushrooms are a source of compounds with promising antitumour activity [1]. We have been working on the identification of wild mushrooms with promising antitumour activity and a Clitocybe alexandri extract which induces cell cycle arrest and apoptosis in a lung cancer cell line has been previously identified by part of the team [2]. The objective of this work was to continue the identification of mushrooms from the Northeast of Portugal with tumour cell growth inhibitory potential. Thirty six wild edible mushrooms were collected and taxonomically identified. Various extracts were prepared and screened for growth inhibitory potential with the SRB assay, in four human tumour cell lines: MCF-7 (breast), NCI-H460 (NSCLC), AGS (gastric) and HCT-15 (colon). These screens allowed the identification of two Suillus species whose methanolic extracts presented potent activity: S. collinitus and S. luteus. The S. collinitus extract was more potent in the MCF-7 cells (GI50=25.2±0.16 µg/ml), causing G1 cell cycle arrest and increasing apoptosis. An increase in p53 and p21 was verified, suggesting that the effect was p53-mediated [3]. The S. luteus extract was slightly more potent in the HCT-15 cells (with mutant p53, GI50=17.8 ± 1.6 µg/ml) than in the other cell lines tested, indicating that its effect was not p53-dependent. In fact, it increased the levels of p53 but the alterations in proteins transactivated by p53 (e.g. Bax) were not consistent with a p53-mediated effect. Growth inhibition in the HCT-15 cells was mostly due to inhibition of cell proliferation and cell cycle arrest in G1, rather than induction of cell death. Finally, this extract had no effect in primary cultures of porcine hepatocytes (GI50>400 µg/ml). In conclusion, these two Suillus species present promising tumour cell growth inhibitory effect and the S. luteus extract may be particularly interesting considering that most tumours present mutant p53. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/3e509d18-7acc-47e8-8eed-0d08f8b3511d/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 5876-PPCDTI |
| id | ipb_95d9dd77f81f106e2ef5256edfb17621 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/8191 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/8191 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Vaz, Josiana A. Vaz, Josiana A. https://www.ciencia-id.pt/171B-88BA-64F5 171B-88BA-64F5 http://orcid.org/0000-0002-6989-1169 0000-0002-6989-1169 Santos, Tiago Tavares, Catarina Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Martins, Anabela Martins, Anabela https://www.ciencia-id.pt/7B18-A810-6B93 7B18-A810-6B93 http://orcid.org/0000-0001-6218-4413 0000-0001-6218-4413 Almeida, Gabriela M. Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Vasconcelos, M. Helena |
| publishDate | 2012 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engporMushrooms are a source of compounds with promising antitumour activity [1]. We have been working on the identification of wild mushrooms with promising antitumour activity and a Clitocybe alexandri extract which induces cell cycle arrest and apoptosis in a lung cancer cell line has been previously identified by part of the team [2]. The objective of this work was to continue the identification of mushrooms from the Northeast of Portugal with tumour cell growth inhibitory potential. Thirty six wild edible mushrooms were collected and taxonomically identified. Various extracts were prepared and screened for growth inhibitory potential with the SRB assay, in four human tumour cell lines: MCF-7 (breast), NCI-H460 (NSCLC), AGS (gastric) and HCT-15 (colon). These screens allowed the identification of two Suillus species whose methanolic extracts presented potent activity: S. collinitus and S. luteus. The S. collinitus extract was more potent in the MCF-7 cells (GI50=25.2±0.16 µg/ml), causing G1 cell cycle arrest and increasing apoptosis. An increase in p53 and p21 was verified, suggesting that the effect was p53-mediated [3]. The S. luteus extract was slightly more potent in the HCT-15 cells (with mutant p53, GI50=17.8 ± 1.6 µg/ml) than in the other cell lines tested, indicating that its effect was not p53-dependent. In fact, it increased the levels of p53 but the alterations in proteins transactivated by p53 (e.g. Bax) were not consistent with a p53-mediated effect. Growth inhibition in the HCT-15 cells was mostly due to inhibition of cell proliferation and cell cycle arrest in G1, rather than induction of cell death. Finally, this extract had no effect in primary cultures of porcine hepatocytes (GI50>400 µg/ml). In conclusion, these two Suillus species present promising tumour cell growth inhibitory effect and the S. luteus extract may be particularly interesting considering that most tumours present mutant p53.application/pdfporTumour cell growth inhibitory potential of mushroom extracts from the genus SuillusPersonalVaz, Josiana A.DSpacehttp://dspace.org/items/1c1a2cb9-5a36-47ba-bca0-ffcbef28df18DSpacehttp://dspace.org/items/1c1a2cb9-5a36-47ba-bca0-ffcbef28df18VazJosiana A.Ciência IDhttps://www.ciencia-id.pt171B-88BA-64F5ORCIDhttp://orcid.org0000-0002-6989-1169Scopus Author IDhttps://www.scopus.com16305323200Santos, TiagoTavares, CatarinaPersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalMartins, AnabelaDSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581DSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581MartinsAnabelaCiência IDhttps://www.ciencia-id.pt7B18-A810-6B93ORCIDhttp://orcid.org0000-0001-6218-4413Researcher IDhttps://www.researcherid.comG-5488-2013Scopus Author IDhttps://www.scopus.com7203013518Almeida, Gabriela M.PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600Vasconcelos, M. HelenaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2013-02-27T15:24:04Z20122012-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/8191http://purl.org/coar/access_right/c_abf2open access888655 bytesFundação para a Ciência e a TecnologiaPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaPOTENCIAL NUTRACEUTICO DE COGUMELOS SILVESTRES DO NORDESTE DE PORTUGAL: ESTUDO DE PROPRIEDADES ANTIO-XIDANTES, ANTIPROLIFERATIVAS E INDUTORAS DA APOPTOSECrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/3e509d18-7acc-47e8-8eed-0d08f8b3511d/download3º Encontro Nacional de Química Terapêutica e 1st Portuguese-Spanish-Brazilian Meeting on Medicinal Chemistry, 28 a 30 de NovembroAveiro |
| spellingShingle | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus Vaz, Josiana A. |
| status | SINGLETON |
| title | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| title_full | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| title_fullStr | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| title_full_unstemmed | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| title_short | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| title_sort | Tumour cell growth inhibitory potential of mushroom extracts from the genus Suillus |
| url | http://hdl.handle.net/10198/8191 |
| visible | 1 |