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Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid

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Resumo:Currently, the food industry is interested in replacing artificial additives by natural ingredients. Some plant extracts have emerged as possible alternatives to artificial preservatives, namely antioxidants. In fact, dairy, meat and bakery products have been developed, incorporating extracts of aromatic plants, spices or fruits, which have antioxidant properties. In this work, the preserving effectiveness of an extract rich in rosmarinic acid was tested in cupcakes and compared to an artificial additive (potassium sorbate, E202). The extract was obtained from Melissa officinalis L. (lemon balm) by applying an ultrasound technique using a mixture of ethanol/water as the extraction solvent. After confirming its antioxidant properties (free radical scavenging effect EC50 = 79 ± 2 μg/mL; reducing power EC50 = 49 ± 1 μg/mL), antimicrobial (against 8 bacteria and 8 food contaminating fungi), and absence of toxicity (in cell lines), it was incorporated in cupcakes, and analysed immediately after incorporation and after 3 and 5 days of storage at room temperature and protected from light. All samples were analysed chromatographically in terms of fatty acids (GC-FID) and free sugars (HPLC-RI). Regarding fatty acids, a total of 21 molecules were identified, with predominance of saturated fatty acids in all cupcakes samples. Individually, palmitic acid and oleic acid were detected in the highest percentages. Among free sugars, sucrose (the major form) and glucose were identified in all samples. The results demonstrate that the addition of the extract rich in rosmarinic acid caused no changes in fatty acids and sugars’ profiles, having the potential to be used in pastry products, meeting the current consumers demand.
Autores principais:Caleja, Cristina
Outros Autores:Barros, Lillian; Barreira, João C.M.; Ćirić, Ana; Soković, Marina; Calhelha, Ricardo C.; Oliveira, Beatriz; Ferreira, Isabel C.F.R.
Assunto:Chromatographic profile
Ano:2018
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Caleja, Cristina
author2 Barros, Lillian
Barreira, João C.M.
Ćirić, Ana
Soković, Marina
Calhelha, Ricardo C.
Oliveira, Beatriz
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
author
author_facet Caleja, Cristina
Barros, Lillian
Barreira, João C.M.
Ćirić, Ana
Soković, Marina
Calhelha, Ricardo C.
Oliveira, Beatriz
Ferreira, Isabel C.F.R.
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Caleja, Cristina\",\"Person.identifier.orcid\":\"0000-0002-5769-8484\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Barreira, João C.M.\",\"Person.identifier.orcid\":\"0000-0003-1233-0990\"},{\"Person.name\":\"Ćirić, Ana\"},{\"Person.name\":\"Soković, Marina\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Oliveira, Beatriz\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Caleja, Cristina
Barros, Lillian
Barreira, João C.M.
Ćirić, Ana
Soković, Marina
Calhelha, Ricardo C.
Oliveira, Beatriz
Ferreira, Isabel C.F.R.
datacite.date.Accepted.fl_str_mv 2018-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2018-10-29T11:08:00Z
datacite.date.embargoed.fl_str_mv 2018-10-29T11:08:00Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Chromatographic profile
datacite.titles.title.fl_str_mv Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Caleja, Cristina
Barros, Lillian
Barreira, João C.M.
Ćirić, Ana
Soković, Marina
Calhelha, Ricardo C.
Oliveira, Beatriz
Ferreira, Isabel C.F.R.
dc.date.Accepted.fl_str_mv 2018-01-01T00:00:00Z
dc.date.available.fl_str_mv 2018-10-29T11:08:00Z
dc.date.embargoed.fl_str_mv 2018-10-29T11:08:00Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/18126
dc.language.none.fl_str_mv eng
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Chromatographic profile
dc.title.fl_str_mv Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description Currently, the food industry is interested in replacing artificial additives by natural ingredients. Some plant extracts have emerged as possible alternatives to artificial preservatives, namely antioxidants. In fact, dairy, meat and bakery products have been developed, incorporating extracts of aromatic plants, spices or fruits, which have antioxidant properties. In this work, the preserving effectiveness of an extract rich in rosmarinic acid was tested in cupcakes and compared to an artificial additive (potassium sorbate, E202). The extract was obtained from Melissa officinalis L. (lemon balm) by applying an ultrasound technique using a mixture of ethanol/water as the extraction solvent. After confirming its antioxidant properties (free radical scavenging effect EC50 = 79 ± 2 μg/mL; reducing power EC50 = 49 ± 1 μg/mL), antimicrobial (against 8 bacteria and 8 food contaminating fungi), and absence of toxicity (in cell lines), it was incorporated in cupcakes, and analysed immediately after incorporation and after 3 and 5 days of storage at room temperature and protected from light. All samples were analysed chromatographically in terms of fatty acids (GC-FID) and free sugars (HPLC-RI). Regarding fatty acids, a total of 21 molecules were identified, with predominance of saturated fatty acids in all cupcakes samples. Individually, palmitic acid and oleic acid were detected in the highest percentages. Among free sugars, sucrose (the major form) and glucose were identified in all samples. The results demonstrate that the addition of the extract rich in rosmarinic acid caused no changes in fatty acids and sugars’ profiles, having the potential to be used in pastry products, meeting the current consumers demand.
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funding.funder.alternateName_str_mv FCT
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funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
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SFRH/BD/93007/2013
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FARH
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person_str_mv Caleja, Cristina
Caleja, Cristina
https://www.ciencia-id.pt/E218-8DD3-4959
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Barros, Lillian
Barros, Lillian
https://www.ciencia-id.pt/9616-35CB-D001
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Barreira, João C.M.
Barreira, João C.M.
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Ćirić, Ana
Soković, Marina
Calhelha, Ricardo C.
Calhelha, Ricardo C.
https://www.ciencia-id.pt/F313-E3CE-554E
F313-E3CE-554E
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Oliveira, Beatriz
Ferreira, Isabel C.F.R.
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spelling engpt_PTCurrently, the food industry is interested in replacing artificial additives by natural ingredients. Some plant extracts have emerged as possible alternatives to artificial preservatives, namely antioxidants. In fact, dairy, meat and bakery products have been developed, incorporating extracts of aromatic plants, spices or fruits, which have antioxidant properties. In this work, the preserving effectiveness of an extract rich in rosmarinic acid was tested in cupcakes and compared to an artificial additive (potassium sorbate, E202). The extract was obtained from Melissa officinalis L. (lemon balm) by applying an ultrasound technique using a mixture of ethanol/water as the extraction solvent. After confirming its antioxidant properties (free radical scavenging effect EC50 = 79 ± 2 μg/mL; reducing power EC50 = 49 ± 1 μg/mL), antimicrobial (against 8 bacteria and 8 food contaminating fungi), and absence of toxicity (in cell lines), it was incorporated in cupcakes, and analysed immediately after incorporation and after 3 and 5 days of storage at room temperature and protected from light. All samples were analysed chromatographically in terms of fatty acids (GC-FID) and free sugars (HPLC-RI). Regarding fatty acids, a total of 21 molecules were identified, with predominance of saturated fatty acids in all cupcakes samples. Individually, palmitic acid and oleic acid were detected in the highest percentages. Among free sugars, sucrose (the major form) and glucose were identified in all samples. The results demonstrate that the addition of the extract rich in rosmarinic acid caused no changes in fatty acids and sugars’ profiles, having the potential to be used in pastry products, meeting the current consumers demand.application/pdfpt_PTChromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acidPersonalCaleja, CristinaDSpacehttp://dspace.org/items/5ef2706d-073c-4c23-9e45-498ac244118cDSpacehttp://dspace.org/items/5ef2706d-073c-4c23-9e45-498ac244118cCalejaCristinaCiência IDhttps://www.ciencia-id.ptE218-8DD3-4959ORCIDhttp://orcid.org0000-0002-5769-8484Scopus Author IDhttps://www.scopus.com36514202500PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalBarreira, João C.M.DSpacehttp://dspace.org/items/4629b12c-39b0-4da8-8b8d-6efba5cf2d81DSpacehttp://dspace.org/items/4629b12c-39b0-4da8-8b8d-6efba5cf2d81BarreiraJoão C.M.ORCIDhttp://orcid.org0000-0003-1233-0990Researcher IDhttps://www.researcherid.comD-8269-2013Scopus Author IDhttps://www.scopus.com54895546900Ćirić, AnaSoković, MarinaPersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333Oliveira, BeatrizPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2018-10-29T11:08:00Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/18126http://purl.org/coar/access_right/c_abf2open accessChromatographic profile1506091 bytesFundação para a Ciência e a TecnologiaAssociated Laboratory for Green Chemistry - Clean Technologies and Processesinfo:eu-repo/grantAgreement/FCT/5876/UID%2FQUI%2F50006%2F2013/PTUID/QUI/50006/20135876Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaIncorporation of natural ingredients in dairy formulations as conservation enhancers and health promotersinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F93007%2F2013/PTSFRH/BD/93007/2013FARHCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2018http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/2e231a88-abf6-427f-9938-c8697c1c230b/downloadUNIFood ConferenceBelgrado, Sérvia
spellingShingle Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
Caleja, Cristina
Chromatographic profile
status SINGLETON
subject.fl_str_mv Chromatographic profile
title Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
title_full Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
title_fullStr Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
title_full_unstemmed Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
title_short Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
title_sort Chromatographic profile of fatty acids and sugars in cupcakes functionalized with an extract rich in rosmarinic acid
topic Chromatographic profile
topic_facet Chromatographic profile
url http://hdl.handle.net/10198/18126
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