Publicação
Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics
| Resumo: | Mentha aquatica (water mint) extracts are regularly used in food flavoring and pharmacology. In the present study, the possible effects of an ethanolic extract from leaves of M. aquatica L. on rat liver mitochondria bioenergetics were evaluated. The plant extract (up to 25 µg.mg protein-1) but not the vehicle, inhibited the mitochondrial oxidative system, as seen by a depression of respiration (state 3, respiratory control ratio (RCR), FCCP-stimulated respiration) and lower generation of the transmembrane electric potential using glutamate + malate or succinate as respiratory substrates. The depressing effects in oxidative phosphorylation can probably be related with the polyphenolic composition of the extract (mainly eriodictyol-7-O-rutinoside, luteolin-7-O-rutinoside, naringenin-7-O-rutinoside, hesperitin-7-O-rutinoside and rosmarinic acid), that can interact with membrane and change the inner mitochondrial membrane lipidic moiety. Despite decreasing the RCR, the presence of M. aquatica extract did not affect the mitochondrial phosphorylative capacity, as estimated by the ADP/O ratio. No significant increase in inner mitochondrial membrane permeability was observed and induction of mitochondrial permeability transition pore was not altered in the range of concentrations tested (up to 25 μg.mg protein-1) either. For the highest concentrations tested (25 μg.mg protein-1 or higher) the inhibition observed on the mitochondrial respiratory chain, as reflected by FCCP-stimulated respiration, revealed that M. aquatica ethanolic extract is toxic for mitochondrial bioenergetics. In conclusion, the present study suggests that a highly daily consumption of an ethanolic extract of M. aquatica leaves should be regarded as hazardous. |
|---|---|
| Autores principais: | Ferreira, Fernanda M. |
| Outros Autores: | Pereira, Olívia R.; Cardoso, Susana M.; Oliveira, Paulo J.; Moreno, António J.M. |
| Assunto: | Mentha aquatica Phenolic composition Mitochondria effects |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172828702310400 |
|---|---|
| author | Ferreira, Fernanda M. |
| author2 | Pereira, Olívia R. Cardoso, Susana M. Oliveira, Paulo J. Moreno, António J.M. |
| author2_role | author author author author |
| author_facet | Ferreira, Fernanda M. Pereira, Olívia R. Cardoso, Susana M. Oliveira, Paulo J. Moreno, António J.M. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Ferreira, Fernanda M.\"},{\"Person.name\":\"Pereira, Olívia R.\",\"Person.identifier.orcid\":\"0000-0002-6275-3134\"},{\"Person.name\":\"Cardoso, Susana M.\"},{\"Person.name\":\"Oliveira, Paulo J.\"},{\"Person.name\":\"Moreno, António J.M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Ferreira, Fernanda M. Pereira, Olívia R. Cardoso, Susana M. Oliveira, Paulo J. Moreno, António J.M. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-07-03T15:12:07Z |
| datacite.date.embargoed.fl_str_mv | 2015-07-03T15:12:07Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Mentha aquatica Phenolic composition Mitochondria effects |
| datacite.titles.title.fl_str_mv | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Ferreira, Fernanda M. Pereira, Olívia R. Cardoso, Susana M. Oliveira, Paulo J. Moreno, António J.M. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-07-03T15:12:07Z |
| dc.date.embargoed.fl_str_mv | 2015-07-03T15:12:07Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/11927 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Instituto Nacional Doutor Ricardo Jorge |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Mentha aquatica Phenolic composition Mitochondria effects |
| dc.title.fl_str_mv | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Mentha aquatica (water mint) extracts are regularly used in food flavoring and pharmacology. In the present study, the possible effects of an ethanolic extract from leaves of M. aquatica L. on rat liver mitochondria bioenergetics were evaluated. The plant extract (up to 25 µg.mg protein-1) but not the vehicle, inhibited the mitochondrial oxidative system, as seen by a depression of respiration (state 3, respiratory control ratio (RCR), FCCP-stimulated respiration) and lower generation of the transmembrane electric potential using glutamate + malate or succinate as respiratory substrates. The depressing effects in oxidative phosphorylation can probably be related with the polyphenolic composition of the extract (mainly eriodictyol-7-O-rutinoside, luteolin-7-O-rutinoside, naringenin-7-O-rutinoside, hesperitin-7-O-rutinoside and rosmarinic acid), that can interact with membrane and change the inner mitochondrial membrane lipidic moiety. Despite decreasing the RCR, the presence of M. aquatica extract did not affect the mitochondrial phosphorylative capacity, as estimated by the ADP/O ratio. No significant increase in inner mitochondrial membrane permeability was observed and induction of mitochondrial permeability transition pore was not altered in the range of concentrations tested (up to 25 μg.mg protein-1) either. For the highest concentrations tested (25 μg.mg protein-1 or higher) the inhibition observed on the mitochondrial respiratory chain, as reflected by FCCP-stimulated respiration, revealed that M. aquatica ethanolic extract is toxic for mitochondrial bioenergetics. In conclusion, the present study suggests that a highly daily consumption of an ethanolic extract of M. aquatica leaves should be regarded as hazardous. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/90617f2f-2a88-4a3b-8ffa-6049aa5b1020/download |
| id | ipb_9b60abb2b78d5742c97ee07fa12ad03e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/11927 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/11927 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Ferreira, Fernanda M. Pereira, Olívia R. Pereira, Olívia R. https://www.ciencia-id.pt/B312-AF21-D2FA B312-AF21-D2FA http://orcid.org/0000-0002-6275-3134 0000-0002-6275-3134 Cardoso, Susana M. Oliveira, Paulo J. Moreno, António J.M. |
| publishDate | 2015 |
| publisher.none.fl_str_mv | Instituto Nacional Doutor Ricardo Jorge |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engInstituto Nacional Doutor Ricardo JorgeporMentha aquatica (water mint) extracts are regularly used in food flavoring and pharmacology. In the present study, the possible effects of an ethanolic extract from leaves of M. aquatica L. on rat liver mitochondria bioenergetics were evaluated. The plant extract (up to 25 µg.mg protein-1) but not the vehicle, inhibited the mitochondrial oxidative system, as seen by a depression of respiration (state 3, respiratory control ratio (RCR), FCCP-stimulated respiration) and lower generation of the transmembrane electric potential using glutamate + malate or succinate as respiratory substrates. The depressing effects in oxidative phosphorylation can probably be related with the polyphenolic composition of the extract (mainly eriodictyol-7-O-rutinoside, luteolin-7-O-rutinoside, naringenin-7-O-rutinoside, hesperitin-7-O-rutinoside and rosmarinic acid), that can interact with membrane and change the inner mitochondrial membrane lipidic moiety. Despite decreasing the RCR, the presence of M. aquatica extract did not affect the mitochondrial phosphorylative capacity, as estimated by the ADP/O ratio. No significant increase in inner mitochondrial membrane permeability was observed and induction of mitochondrial permeability transition pore was not altered in the range of concentrations tested (up to 25 μg.mg protein-1) either. For the highest concentrations tested (25 μg.mg protein-1 or higher) the inhibition observed on the mitochondrial respiratory chain, as reflected by FCCP-stimulated respiration, revealed that M. aquatica ethanolic extract is toxic for mitochondrial bioenergetics. In conclusion, the present study suggests that a highly daily consumption of an ethanolic extract of M. aquatica leaves should be regarded as hazardous.application/pdfporPotential noxious effect of mentha aquatica L. on mitochondrial bioenergeticsFerreira, Fernanda M.PersonalPereira, Olívia R.DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453PereiraOlívia R.Ciência IDhttps://www.ciencia-id.ptB312-AF21-D2FAORCIDhttp://orcid.org0000-0002-6275-3134Researcher IDhttps://www.researcherid.comH-1049-2014Scopus Author IDhttps://www.scopus.com52264387000Cardoso, Susana M.Oliveira, Paulo J.Moreno, António J.M.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2015-07-03T15:12:07Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/11927http://purl.org/coar/access_right/c_abf2open accessMentha aquaticaPhenolic compositionMitochondria effects1511273 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/90617f2f-2a88-4a3b-8ffa-6049aa5b1020/downloadInternational Conference on Food Contaminants: challenges in chemical mixtures171172Lisboa, Portugal |
| spellingShingle | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics Ferreira, Fernanda M. Mentha aquatica Phenolic composition Mitochondria effects |
| status | SINGLETON |
| subject.fl_str_mv | Mentha aquatica Phenolic composition Mitochondria effects |
| title | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| title_full | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| title_fullStr | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| title_full_unstemmed | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| title_short | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| title_sort | Potential noxious effect of mentha aquatica L. on mitochondrial bioenergetics |
| topic | Mentha aquatica Phenolic composition Mitochondria effects |
| topic_facet | Mentha aquatica Phenolic composition Mitochondria effects |
| url | http://hdl.handle.net/10198/11927 |
| visible | 1 |