Publicação
Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants
| Resumo: | The consumers' interest for a healthier diet and the use of natural products in the prevention and/or treatment of health problems has triggered an intensive search for bioactive plant compounds. Plants are a good source of phenolic compounds, terpenoids, alkaloids, quinones, carotenoids, sterols, glucosinolates and other compounds, which provide health benefits due to their wide range of biological properties. This work aimed to evaluate the effects of gamma radiation on the phenolic composition, cytotoxicity, virucidal and antimicrobial properties of Aloysia citrodora L. and Mentha x piperita L. infusions. Gamma radiation treatment was performed in a 60Co chamber, applying the doses of 1 and 10 kGy and the results were compared with the control sample (0 kGy). The phenolic compounds profile was obtained by HPLC-DAD-ESI/MS and the cytotoxicity was evaluated in human tumour cell lines and in a primary cell culture. For virucidal efficacy evaluation, MNV-1 and HAdV-5 viral titers were determined by plaque assay in Raw264.7 and A549 cell lines, respectively. The antibacterial activity was evaluated against Gram-negative and Gram-positive bacteria. Radiation treatment has been shown to cause different effects on phenolic concentrations and virucidal efficacy, depending on the plant species studied, the virus and the absorbed dose. In cytotoxicity assay A. citrodora did not reveal significant statistically differences considering the different doses applied, however M. piperita at 10 kGy stood out with the highest cytotoxic potential in all tested cell lines. Regarding antibacterial assays, S. aureus was the only bacteria that was sensible to the infusions of non-irradiated and 1 kGy irradiated in both species. Thus, this study contribute to a wider knowledge of the effects of gamma irradiation on several relevant bioactive potentials of some aromatic and medicinal plants and showed that gamma radiation is a technique recommended for these matrices. |
|---|---|
| Autores principais: | Pereira, Eliana |
| Outros Autores: | Pimenta, Andreia I.; Calhelha, Ricardo C.; Antonio, Amilcar L.; Barros, Lillian; Santos-Buelga, Celestino; Cabo Verde, Sandra; Ferreira, Isabel C.F.R. |
| Assunto: | Gamma radiation |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851191785488384 |
|---|---|
| author | Pereira, Eliana |
| author2 | Pimenta, Andreia I. Calhelha, Ricardo C. Antonio, Amilcar L. Barros, Lillian Santos-Buelga, Celestino Cabo Verde, Sandra Ferreira, Isabel C.F.R. |
| author2_role | author author author author author author author |
| author_facet | Pereira, Eliana Pimenta, Andreia I. Calhelha, Ricardo C. Antonio, Amilcar L. Barros, Lillian Santos-Buelga, Celestino Cabo Verde, Sandra Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Pereira, Eliana\",\"Person.identifier.orcid\":\"0000-0003-2854-6745\"},{\"Person.name\":\"Pimenta, Andreia I.\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Antonio, Amilcar L.\",\"Person.identifier.orcid\":\"0000-0001-8271-9964\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Santos-Buelga, Celestino\"},{\"Person.name\":\"Cabo Verde, Sandra\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pereira, Eliana Pimenta, Andreia I. Calhelha, Ricardo C. Antonio, Amilcar L. Barros, Lillian Santos-Buelga, Celestino Cabo Verde, Sandra Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-10-29T11:01:54Z |
| datacite.date.embargoed.fl_str_mv | 2018-10-29T11:01:54Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Gamma radiation |
| datacite.titles.title.fl_str_mv | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pereira, Eliana Pimenta, Andreia I. Calhelha, Ricardo C. Antonio, Amilcar L. Barros, Lillian Santos-Buelga, Celestino Cabo Verde, Sandra Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-10-29T11:01:54Z |
| dc.date.embargoed.fl_str_mv | 2018-10-29T11:01:54Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/18125 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Gamma radiation |
| dc.title.fl_str_mv | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | The consumers' interest for a healthier diet and the use of natural products in the prevention and/or treatment of health problems has triggered an intensive search for bioactive plant compounds. Plants are a good source of phenolic compounds, terpenoids, alkaloids, quinones, carotenoids, sterols, glucosinolates and other compounds, which provide health benefits due to their wide range of biological properties. This work aimed to evaluate the effects of gamma radiation on the phenolic composition, cytotoxicity, virucidal and antimicrobial properties of Aloysia citrodora L. and Mentha x piperita L. infusions. Gamma radiation treatment was performed in a 60Co chamber, applying the doses of 1 and 10 kGy and the results were compared with the control sample (0 kGy). The phenolic compounds profile was obtained by HPLC-DAD-ESI/MS and the cytotoxicity was evaluated in human tumour cell lines and in a primary cell culture. For virucidal efficacy evaluation, MNV-1 and HAdV-5 viral titers were determined by plaque assay in Raw264.7 and A549 cell lines, respectively. The antibacterial activity was evaluated against Gram-negative and Gram-positive bacteria. Radiation treatment has been shown to cause different effects on phenolic concentrations and virucidal efficacy, depending on the plant species studied, the virus and the absorbed dose. In cytotoxicity assay A. citrodora did not reveal significant statistically differences considering the different doses applied, however M. piperita at 10 kGy stood out with the highest cytotoxic potential in all tested cell lines. Regarding antibacterial assays, S. aureus was the only bacteria that was sensible to the infusions of non-irradiated and 1 kGy irradiated in both species. Thus, this study contribute to a wider knowledge of the effects of gamma irradiation on several relevant bioactive potentials of some aromatic and medicinal plants and showed that gamma radiation is a technique recommended for these matrices. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
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| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | RECI/AAG-TEC/0400/2012 UID/AGR/00690/2013 |
| funding.name_str_mv | 3599-PPCDT 6817 - DCRRNI ID |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/18125 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/18125 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pereira, Eliana Pereira, Eliana https://www.ciencia-id.pt/BE17-462D-9A75 BE17-462D-9A75 http://orcid.org/0000-0003-2854-6745 0000-0003-2854-6745 Pimenta, Andreia I. Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Antonio, Amilcar L. Antonio, Amilcar L. https://www.ciencia-id.pt/1014-5259-DE86 1014-5259-DE86 http://orcid.org/0000-0001-8271-9964 0000-0001-8271-9964 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Santos-Buelga, Celestino Cabo Verde, Sandra Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2018 |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engpt_PTThe consumers' interest for a healthier diet and the use of natural products in the prevention and/or treatment of health problems has triggered an intensive search for bioactive plant compounds. Plants are a good source of phenolic compounds, terpenoids, alkaloids, quinones, carotenoids, sterols, glucosinolates and other compounds, which provide health benefits due to their wide range of biological properties. This work aimed to evaluate the effects of gamma radiation on the phenolic composition, cytotoxicity, virucidal and antimicrobial properties of Aloysia citrodora L. and Mentha x piperita L. infusions. Gamma radiation treatment was performed in a 60Co chamber, applying the doses of 1 and 10 kGy and the results were compared with the control sample (0 kGy). The phenolic compounds profile was obtained by HPLC-DAD-ESI/MS and the cytotoxicity was evaluated in human tumour cell lines and in a primary cell culture. For virucidal efficacy evaluation, MNV-1 and HAdV-5 viral titers were determined by plaque assay in Raw264.7 and A549 cell lines, respectively. The antibacterial activity was evaluated against Gram-negative and Gram-positive bacteria. Radiation treatment has been shown to cause different effects on phenolic concentrations and virucidal efficacy, depending on the plant species studied, the virus and the absorbed dose. In cytotoxicity assay A. citrodora did not reveal significant statistically differences considering the different doses applied, however M. piperita at 10 kGy stood out with the highest cytotoxic potential in all tested cell lines. Regarding antibacterial assays, S. aureus was the only bacteria that was sensible to the infusions of non-irradiated and 1 kGy irradiated in both species. Thus, this study contribute to a wider knowledge of the effects of gamma irradiation on several relevant bioactive potentials of some aromatic and medicinal plants and showed that gamma radiation is a technique recommended for these matrices.application/pdfpt_PTEffects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plantsPersonalPereira, ElianaDSpacehttp://dspace.org/items/dda60cdc-0a2a-4c10-863e-eaef1e6f1fb9DSpacehttp://dspace.org/items/dda60cdc-0a2a-4c10-863e-eaef1e6f1fb9PereiraElianaCiência IDhttps://www.ciencia-id.ptBE17-462D-9A75ORCIDhttp://orcid.org0000-0003-2854-6745Researcher IDhttps://www.researcherid.comQ-8017-2018Scopus Author IDhttps://www.scopus.com55938912300Pimenta, Andreia I.PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalAntonio, Amilcar L.DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28AntonioAmilcar L.Ciência IDhttps://www.ciencia-id.pt1014-5259-DE86ORCIDhttp://orcid.org0000-0001-8271-9964Researcher IDhttps://www.researcherid.comJ-1960-2014Scopus Author IDhttps://www.scopus.com44560921900PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801Santos-Buelga, CelestinoCabo Verde, SandraPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2018-10-29T11:01:54Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/18125http://purl.org/coar/access_right/c_abf2open accessGamma radiation1101270 bytesFundação para a Ciência e a TecnologiaApplication of Ionizing Radiation for a Sustainable Environmentinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/RECI%2FAAG-TEC%2F0400%2F2012/PTRECI/AAG-TEC/0400/20123599-PPCDTCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Centreinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAGR%2F00690%2F2013/PTUID/AGR/00690/20136817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2018http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/489d2fdf-bc72-45f5-a8aa-1a2d5e35523c/downloadUNIFood ConferenceBelgrado, Sérvia |
| spellingShingle | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants Pereira, Eliana Gamma radiation |
| subject.fl_str_mv | Gamma radiation |
| title | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| title_full | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| title_fullStr | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| title_full_unstemmed | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| title_short | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| title_sort | Effects of gamma radiation on phenolic composition, cytotoxicity, antibacterial and virucidal activities of two medicinal plants |
| topic | Gamma radiation |
| topic_facet | Gamma radiation |
| url | http://hdl.handle.net/10198/18125 |
| visible | 1 |
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