Publicação
Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro
| Resumo: | Stevia rebaudiana Bertoni leaves are natural sources of steviol glycosides, which are used commercially for sweetening and flavouring foods and beverages. Steviol glycosides are natural sweeteners constituting an alternative to synthetic compounds like cyclamates or aspartame. S. rebaudiana has been produced mainly for its stevioside compounds but it contains other metabolites with potential therapeutic benefits such as alkaloids, hydroxycinnamic acids, oligosaccharides or essential oils. The chemical profile of samples development by micropropagation or in green house or in field conditions, should be characterized to ensure the quality of the samples supplied by Stevia producers. Multiplication rate and fresh weight were determined for plants micropropagated in two different culture media (medium A - Murashige and Skoog (MS) without hormones and sucrose), (medium B - MS with 0.5 mg.L-1 of kinetin and 20 g.L-1of sucrose). Apart from spontaneous rooting rate determination, induction of plant rooting by auxin shock, using indole-3-butyric acid (IBA) (2 mg.mL-1), was also evaluated. Acclimatization in greenhouse was performed with hydro atomization nozzles working every 10 minutes. Plants on the field were fertilized by a nutrient solution with N, P2O5, K2O and B. The essential oil yield was determined for plants in all conditions (in vitro, greenhouse, field) using a Clevenger-type apparatus. Volatiles were isolated using a Likens-Nickerson apparatus and analyzed by GCMS. The in vitro multiplication rate was 300% per month and the fresh weight after a 4 week subculture was 0.9 g. Spontaneous rooting rate was less than 4% after 4 months but induced rooting achieved 30% of plants with developed root system after 1 week and 70% after 2 weeks. Acclimatization rate was 100% after 2 weeks. The essential oil yield was <0.06% for all samples. Volatiles identification revealed identical composition in all samples, with α-pinene (11-31%), bicyclogermacrene (5‑19%), trans-β-farnesene (7-15%), β-elemene (6-10%) and β-caryophyllene (3-10%) as major compounds. Quantitative differences were noteworthy. |
|---|---|
| Autores principais: | Barroso, Marisa |
| Outros Autores: | Goes, Hugo; Rodrigues, M.A.; Arrobas, Margarida; Pedro, Luís G.; Sousa, Maria João |
| Assunto: | Stevia |
| Ano: | 2016 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173149411377152 |
|---|---|
| author | Barroso, Marisa |
| author2 | Goes, Hugo Rodrigues, M.A. Arrobas, Margarida Pedro, Luís G. Sousa, Maria João |
| author2_role | author author author author author |
| author_facet | Barroso, Marisa Goes, Hugo Rodrigues, M.A. Arrobas, Margarida Pedro, Luís G. Sousa, Maria João |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Barroso, Marisa\"},{\"Person.name\":\"Goes, Hugo\"},{\"Person.name\":\"Rodrigues, M.A.\",\"Person.identifier.orcid\":\"0000-0002-5367-1129\"},{\"Person.name\":\"Arrobas, Margarida\",\"Person.identifier.orcid\":\"0000-0002-4652-485X\"},{\"Person.name\":\"Pedro, Luís G.\"},{\"Person.name\":\"Sousa, Maria João\",\"Person.identifier.orcid\":\"0000-0002-9946-4926\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Barroso, Marisa Goes, Hugo Rodrigues, M.A. Arrobas, Margarida Pedro, Luís G. Sousa, Maria João |
| datacite.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-02-06T12:22:26Z |
| datacite.date.embargoed.fl_str_mv | 2018-02-06T12:22:26Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Stevia |
| datacite.titles.title.fl_str_mv | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Barroso, Marisa Goes, Hugo Rodrigues, M.A. Arrobas, Margarida Pedro, Luís G. Sousa, Maria João |
| dc.date.Accepted.fl_str_mv | 2016-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-02-06T12:22:26Z |
| dc.date.embargoed.fl_str_mv | 2018-02-06T12:22:26Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/15574 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Stevia |
| dc.title.fl_str_mv | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Stevia rebaudiana Bertoni leaves are natural sources of steviol glycosides, which are used commercially for sweetening and flavouring foods and beverages. Steviol glycosides are natural sweeteners constituting an alternative to synthetic compounds like cyclamates or aspartame. S. rebaudiana has been produced mainly for its stevioside compounds but it contains other metabolites with potential therapeutic benefits such as alkaloids, hydroxycinnamic acids, oligosaccharides or essential oils. The chemical profile of samples development by micropropagation or in green house or in field conditions, should be characterized to ensure the quality of the samples supplied by Stevia producers. Multiplication rate and fresh weight were determined for plants micropropagated in two different culture media (medium A - Murashige and Skoog (MS) without hormones and sucrose), (medium B - MS with 0.5 mg.L-1 of kinetin and 20 g.L-1of sucrose). Apart from spontaneous rooting rate determination, induction of plant rooting by auxin shock, using indole-3-butyric acid (IBA) (2 mg.mL-1), was also evaluated. Acclimatization in greenhouse was performed with hydro atomization nozzles working every 10 minutes. Plants on the field were fertilized by a nutrient solution with N, P2O5, K2O and B. The essential oil yield was determined for plants in all conditions (in vitro, greenhouse, field) using a Clevenger-type apparatus. Volatiles were isolated using a Likens-Nickerson apparatus and analyzed by GCMS. The in vitro multiplication rate was 300% per month and the fresh weight after a 4 week subculture was 0.9 g. Spontaneous rooting rate was less than 4% after 4 months but induced rooting achieved 30% of plants with developed root system after 1 week and 70% after 2 weeks. Acclimatization rate was 100% after 2 weeks. The essential oil yield was <0.06% for all samples. Volatiles identification revealed identical composition in all samples, with α-pinene (11-31%), bicyclogermacrene (5‑19%), trans-β-farnesene (7-15%), β-elemene (6-10%) and β-caryophyllene (3-10%) as major compounds. Quantitative differences were noteworthy. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/96f2b72b-cd3b-47a7-bbdb-54f6cdc60d6b/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/15574 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/15574 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Barroso, Marisa Goes, Hugo Rodrigues, M.A. Rodrigues, M.A. https://www.ciencia-id.pt/371D-DF0D-8D68 371D-DF0D-8D68 http://orcid.org/0000-0002-5367-1129 0000-0002-5367-1129 Arrobas, Margarida Arrobas, Margarida https://www.ciencia-id.pt/971C-B85B-1EC0 971C-B85B-1EC0 http://orcid.org/0000-0002-4652-485X 0000-0002-4652-485X Pedro, Luís G. Sousa, Maria João Sousa, Maria João https://www.ciencia-id.pt/D91E-3571-3979 D91E-3571-3979 http://orcid.org/0000-0002-9946-4926 0000-0002-9946-4926 |
| publishDate | 2016 |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engpt_PTStevia rebaudiana Bertoni leaves are natural sources of steviol glycosides, which are used commercially for sweetening and flavouring foods and beverages. Steviol glycosides are natural sweeteners constituting an alternative to synthetic compounds like cyclamates or aspartame. S. rebaudiana has been produced mainly for its stevioside compounds but it contains other metabolites with potential therapeutic benefits such as alkaloids, hydroxycinnamic acids, oligosaccharides or essential oils. The chemical profile of samples development by micropropagation or in green house or in field conditions, should be characterized to ensure the quality of the samples supplied by Stevia producers. Multiplication rate and fresh weight were determined for plants micropropagated in two different culture media (medium A - Murashige and Skoog (MS) without hormones and sucrose), (medium B - MS with 0.5 mg.L-1 of kinetin and 20 g.L-1of sucrose). Apart from spontaneous rooting rate determination, induction of plant rooting by auxin shock, using indole-3-butyric acid (IBA) (2 mg.mL-1), was also evaluated. Acclimatization in greenhouse was performed with hydro atomization nozzles working every 10 minutes. Plants on the field were fertilized by a nutrient solution with N, P2O5, K2O and B. The essential oil yield was determined for plants in all conditions (in vitro, greenhouse, field) using a Clevenger-type apparatus. Volatiles were isolated using a Likens-Nickerson apparatus and analyzed by GCMS. The in vitro multiplication rate was 300% per month and the fresh weight after a 4 week subculture was 0.9 g. Spontaneous rooting rate was less than 4% after 4 months but induced rooting achieved 30% of plants with developed root system after 1 week and 70% after 2 weeks. Acclimatization rate was 100% after 2 weeks. The essential oil yield was <0.06% for all samples. Volatiles identification revealed identical composition in all samples, with α-pinene (11-31%), bicyclogermacrene (5‑19%), trans-β-farnesene (7-15%), β-elemene (6-10%) and β-caryophyllene (3-10%) as major compounds. Quantitative differences were noteworthy.application/pdfpt_PTStevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitroBarroso, MarisaGoes, HugoPersonalRodrigues, M.A.DSpacehttp://dspace.org/items/43621353-fa11-4559-9b24-27eba5ad3de0DSpacehttp://dspace.org/items/43621353-fa11-4559-9b24-27eba5ad3de0RodriguesManuel ÂngeloCiência IDhttps://www.ciencia-id.pt371D-DF0D-8D68ORCIDhttp://orcid.org0000-0002-5367-1129Researcher IDhttps://www.researcherid.comO-1721-2016Scopus Author IDhttps://www.scopus.com35270106800PersonalArrobas, MargaridaDSpacehttp://dspace.org/items/d057ae6b-331c-4980-967d-0ce4fe1bcf8aDSpacehttp://dspace.org/items/d057ae6b-331c-4980-967d-0ce4fe1bcf8aArrobasMargaridaCiência IDhttps://www.ciencia-id.pt971C-B85B-1EC0ORCIDhttp://orcid.org0000-0002-4652-485XScopus Author IDhttps://www.scopus.com8575728800Pedro, Luís G.PersonalSousa, Maria JoãoDSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0DSpacehttp://dspace.org/items/da5c7615-9844-4fdf-b4d4-64d0c8e72de0SousaMaria JoãoCiência IDhttps://www.ciencia-id.ptD91E-3571-3979ORCIDhttp://orcid.org0000-0002-9946-4926Scopus Author IDhttps://www.scopus.com55230282700HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2018-02-06T12:22:26Z20162016-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/15574http://purl.org/coar/access_right/c_abf2open accessStevia1180731 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2016http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/96f2b72b-cd3b-47a7-bbdb-54f6cdc60d6b/download1st European Conference of Post graduate Horticulture Scientists1616Palermo |
| spellingShingle | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro Barroso, Marisa Stevia |
| status | SINGLETON |
| subject.fl_str_mv | Stevia |
| title | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| title_full | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| title_fullStr | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| title_full_unstemmed | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| title_short | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| title_sort | Stevia rebaudiana: a study in the volatiles profile from plants grown in the field in the greenhouse and micropropagated in vitro |
| topic | Stevia |
| topic_facet | Stevia |
| url | http://hdl.handle.net/10198/15574 |
| visible | 1 |