Publicação
Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties
| Resumo: | The cultivation of grapevines (Vitis vinifera L.) has extended worldwide, with great economic importance. From this crop, we can obtain grapes for fresh consumption, raisins, juices, wine, and other derived products. The cultivation of grapevines also generates some byproducts, such as seeds, skins, wood and leaves. The leaves can be removed from the plant, as is common agricultural practice, to improve the production and quality of the grapes at certain periods in the crop cycle. In the present work, the phytochemical composition (total phenols content, hydroxycinnamic acids derivatives and flavonols) and antioxidant potential (reducing power, ABTS and DPPH radical scavenging capacity) of grapevine leaves from cv. Touriga Nacional were evaluated. The studied cultivar was under three severities of regulated deficit irrigation (RDI60, RDI40, and RDI20; providing 60, 40 and 20% of reference evapotranspiration, respectively) and non-irrigation (NI; exclusively rain-fed), in a vineyard located in the Demarcated Douro Region (Portugal). The leaves were collected at three different phenological stages: veraison, maturation, and grape harvest. For the first time, the interactions between different irrigation regimes and the collection period were evaluated on grapevine leaves. The results obtained for the phytochemical composition revealed that increasing irrigation reduced total phenols content, hydroxycinnamic acids derivatives, and flavonols in all of the phenological stages studied. For instance, compared to that of the control treatment (NI), the total phenols content reduced 19% in RDI20 (from 189 mg GAE g−1 to 153 mg GAE g−1), 28% in RDI40 (136 mg GAE g−1), and 33% in RDI60 (127 mg GAE g−1). The antioxidant activity suffered a reduction when the irrigation amount was increased for all assays. Additionally, the antioxidant activity suffered a reduction over time, presenting higher values in veraison. For harvest sampling, the loss of antioxidant activity was more notable, with EC50 values varying between 0.092 mg mL−1 (NI) and 0.187 mg mL−1 (RDI60). Altogether, the results suggest that grapevine leaves are a potential source of natural compounds with valuable antioxidant properties that could be explored by the pharmaceutical, chemical and food industries. |
|---|---|
| Autores principais: | Barreales, David |
| Outros Autores: | Malheiro, Ricardo; Pereira, J.A.; Andrade, João Verdial; Bento, Albino; Casquero, Pedro Antonio; Ribeiro, A.C. |
| Assunto: | Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850679737516032 |
|---|---|
| author | Barreales, David |
| author2 | Malheiro, Ricardo Pereira, J.A. Andrade, João Verdial Bento, Albino Casquero, Pedro Antonio Ribeiro, A.C. |
| author2_role | author author author author author author |
| author_facet | Barreales, David Malheiro, Ricardo Pereira, J.A. Andrade, João Verdial Bento, Albino Casquero, Pedro Antonio Ribeiro, A.C. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Barreales, David\",\"Person.identifier.orcid\":\"0000-0002-8425-0167\"},{\"Person.name\":\"Malheiro, Ricardo\",\"Person.identifier.orcid\":\"0000-0002-7342-0511\"},{\"Person.name\":\"Pereira, J.A.\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\"},{\"Person.name\":\"Andrade, João Verdial\",\"Person.identifier.orcid\":\"0000-0002-5809-413X\"},{\"Person.name\":\"Bento, Albino\",\"Person.identifier.orcid\":\"0000-0001-5215-785X\"},{\"Person.name\":\"Casquero, Pedro Antonio\"},{\"Person.name\":\"Ribeiro, A.C.\",\"Person.identifier.orcid\":\"0000-0002-8280-9027\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Barreales, David Malheiro, Ricardo Pereira, J.A. Andrade, João Verdial Bento, Albino Casquero, Pedro Antonio Ribeiro, A.C. |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| datacite.titles.title.fl_str_mv | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Barreales, David Malheiro, Ricardo Pereira, J.A. Andrade, João Verdial Bento, Albino Casquero, Pedro Antonio Ribeiro, A.C. |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/11275 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| dc.title.fl_str_mv | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The cultivation of grapevines (Vitis vinifera L.) has extended worldwide, with great economic importance. From this crop, we can obtain grapes for fresh consumption, raisins, juices, wine, and other derived products. The cultivation of grapevines also generates some byproducts, such as seeds, skins, wood and leaves. The leaves can be removed from the plant, as is common agricultural practice, to improve the production and quality of the grapes at certain periods in the crop cycle. In the present work, the phytochemical composition (total phenols content, hydroxycinnamic acids derivatives and flavonols) and antioxidant potential (reducing power, ABTS and DPPH radical scavenging capacity) of grapevine leaves from cv. Touriga Nacional were evaluated. The studied cultivar was under three severities of regulated deficit irrigation (RDI60, RDI40, and RDI20; providing 60, 40 and 20% of reference evapotranspiration, respectively) and non-irrigation (NI; exclusively rain-fed), in a vineyard located in the Demarcated Douro Region (Portugal). The leaves were collected at three different phenological stages: veraison, maturation, and grape harvest. For the first time, the interactions between different irrigation regimes and the collection period were evaluated on grapevine leaves. The results obtained for the phytochemical composition revealed that increasing irrigation reduced total phenols content, hydroxycinnamic acids derivatives, and flavonols in all of the phenological stages studied. For instance, compared to that of the control treatment (NI), the total phenols content reduced 19% in RDI20 (from 189 mg GAE g−1 to 153 mg GAE g−1), 28% in RDI40 (136 mg GAE g−1), and 33% in RDI60 (127 mg GAE g−1). The antioxidant activity suffered a reduction when the irrigation amount was increased for all assays. Additionally, the antioxidant activity suffered a reduction over time, presenting higher values in veraison. For harvest sampling, the loss of antioxidant activity was more notable, with EC50 values varying between 0.092 mg mL−1 (NI) and 0.187 mg mL−1 (RDI60). Altogether, the results suggest that grapevine leaves are a potential source of natural compounds with valuable antioxidant properties that could be explored by the pharmaceutical, chemical and food industries. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/3146ca64-59ed-4a51-bf8b-811e19d5617d/download |
| id | ipb_a4f64045e2fec32193ae4fd65d9b4ddf |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/11275 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/11275 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Barreales, David Barreales, David https://www.ciencia-id.pt/FA16-8DE7-6968 FA16-8DE7-6968 http://orcid.org/0000-0002-8425-0167 0000-0002-8425-0167 Malheiro, Ricardo Malheiro, Ricardo http://orcid.org/0000-0002-7342-0511 0000-0002-7342-0511 Pereira, J.A. Pereira, J.A. https://www.ciencia-id.pt/611F-80B2-A7C1 611F-80B2-A7C1 http://orcid.org/0000-0002-2260-0600 0000-0002-2260-0600 Andrade, João Verdial Andrade, João Verdial http://orcid.org/0000-0002-5809-413X 0000-0002-5809-413X Bento, Albino Bento, Albino https://www.ciencia-id.pt/D516-325A-9AD7 D516-325A-9AD7 http://orcid.org/0000-0001-5215-785X 0000-0001-5215-785X Casquero, Pedro Antonio Ribeiro, A.C. Ribeiro, A.C. https://www.ciencia-id.pt/9D19-E833-BE97 9D19-E833-BE97 http://orcid.org/0000-0002-8280-9027 0000-0002-8280-9027 |
| publishDate | 2019 |
| reponame_str | Biblioteca Digital do IPB |
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| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engThe cultivation of grapevines (Vitis vinifera L.) has extended worldwide, with great economic importance. From this crop, we can obtain grapes for fresh consumption, raisins, juices, wine, and other derived products. The cultivation of grapevines also generates some byproducts, such as seeds, skins, wood and leaves. The leaves can be removed from the plant, as is common agricultural practice, to improve the production and quality of the grapes at certain periods in the crop cycle. In the present work, the phytochemical composition (total phenols content, hydroxycinnamic acids derivatives and flavonols) and antioxidant potential (reducing power, ABTS and DPPH radical scavenging capacity) of grapevine leaves from cv. Touriga Nacional were evaluated. The studied cultivar was under three severities of regulated deficit irrigation (RDI60, RDI40, and RDI20; providing 60, 40 and 20% of reference evapotranspiration, respectively) and non-irrigation (NI; exclusively rain-fed), in a vineyard located in the Demarcated Douro Region (Portugal). The leaves were collected at three different phenological stages: veraison, maturation, and grape harvest. For the first time, the interactions between different irrigation regimes and the collection period were evaluated on grapevine leaves. The results obtained for the phytochemical composition revealed that increasing irrigation reduced total phenols content, hydroxycinnamic acids derivatives, and flavonols in all of the phenological stages studied. For instance, compared to that of the control treatment (NI), the total phenols content reduced 19% in RDI20 (from 189 mg GAE g−1 to 153 mg GAE g−1), 28% in RDI40 (136 mg GAE g−1), and 33% in RDI60 (127 mg GAE g−1). The antioxidant activity suffered a reduction when the irrigation amount was increased for all assays. Additionally, the antioxidant activity suffered a reduction over time, presenting higher values in veraison. For harvest sampling, the loss of antioxidant activity was more notable, with EC50 values varying between 0.092 mg mL−1 (NI) and 0.187 mg mL−1 (RDI60). Altogether, the results suggest that grapevine leaves are a potential source of natural compounds with valuable antioxidant properties that could be explored by the pharmaceutical, chemical and food industries.application/pdfen_ENEffects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant propertiesPersonalBarreales, DavidDSpacehttp://dspace.org/items/44d42f8e-7185-4c33-9551-66928841ce3eDSpacehttp://dspace.org/items/44d42f8e-7185-4c33-9551-66928841ce3eBarrealesDavidCiência IDhttps://www.ciencia-id.ptFA16-8DE7-6968ORCIDhttp://orcid.org0000-0002-8425-0167Scopus Author IDhttps://www.scopus.com57202136257PersonalMalheiro, RicardoDSpacehttp://dspace.org/items/0fa7974d-abd3-444b-9a7e-16d16530a0f7DSpacehttp://dspace.org/items/0fa7974d-abd3-444b-9a7e-16d16530a0f7MalheiroRicardoORCIDhttp://orcid.org0000-0002-7342-0511Scopus Author IDhttps://www.scopus.com25650218300PersonalPereira, J.A.DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348PersonalAndrade, João VerdialDSpacehttp://dspace.org/items/8da5730a-fd13-4d9e-92a9-097b8156a42eDSpacehttp://dspace.org/items/8da5730a-fd13-4d9e-92a9-097b8156a42eAndradeJoão VerdialORCIDhttp://orcid.org0000-0002-5809-413XScopus Author IDhttps://www.scopus.com55210790800PersonalBento, AlbinoDSpacehttp://dspace.org/items/233115be-9d46-49d0-8b7d-2d64406d64a0DSpacehttp://dspace.org/items/233115be-9d46-49d0-8b7d-2d64406d64a0BentoAlbinoCiência IDhttps://www.ciencia-id.ptD516-325A-9AD7ORCIDhttp://orcid.org0000-0001-5215-785XResearcher IDhttps://www.researcherid.comN-9706-2016Scopus Author IDhttps://www.scopus.com35247694000Casquero, Pedro AntonioPersonalRibeiro, A.C.DSpacehttp://dspace.org/items/b7ecf809-561e-40ce-86b0-603a60433710DSpacehttp://dspace.org/items/b7ecf809-561e-40ce-86b0-603a60433710RibeiroAntónio C.Ciência IDhttps://www.ciencia-id.pt9D19-E833-BE97ORCIDhttp://orcid.org0000-0002-8280-9027HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0304-4238DOIIsPartOf10.1016/j.scienta.2018.09.0732018-01-19T10:00:00Z20192019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/11275http://purl.org/coar/access_right/c_abf2open accessDeficit irrigationLeavesTotal antioxidant capacityVitis vinifera L.785372 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/3146ca64-59ed-4a51-bf8b-811e19d5617d/download |
| spellingShingle | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties Barreales, David Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| subject.fl_str_mv | Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| title | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| title_full | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| title_fullStr | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| title_full_unstemmed | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| title_short | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| title_sort | Effects of irrigation and collection period on grapevine leaf (Vitis vinifera L. var. Touriga Nacional): Evaluation of the phytochemical composition and antioxidant properties |
| topic | Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| topic_facet | Deficit irrigation Leaves Total antioxidant capacity Vitis vinifera L. |
| url | http://hdl.handle.net/10198/11275 |
| visible | 1 |