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Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies

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Resumo:Mushrooms are very perishable food products and tend to lose quality immediately after harveStr. Drying is the most common method for preserving mushrooms, freezing is becoming increasingly popular and food irradiation has also been suggested by many researchers as a good conservation technique in order to maintain and increase the food shelf life. In the present work, the effects of different processing technologies (freezing, drying and gamma irradiation) on antioxidant activity of the wild mushroom Macrolepiota procera were evaluated. Fruiting bodies were obtained in Trás-os-Montes, in the Northeast of Portugal, in November 2011. The irradiation was performed in experimental equipment with four 60Co sources at 0.6 kGy. The samples were submitted to different processing technologies: freezing (at -20 ºC in a freezer), drying (at 30 ºC in an oven) and gamma irradiation in fresh samples. Antioxidant activity was determined in the methanolic extracts by in vitro assays measuring DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) assay. Total phenolics were also determined by the Folin-Ciocalteu assay. Dried samples gave the highest DPPH scavenging activity (50% at 2.7 mg/mL), β-carotene bleaching inhibition (50% at 1.10 mg/mL) and the highest phenolic content (19.2 mg GAE/g methanolic extract). Frozen and irradiated samples presented the highest reducing power (0.5 absorbance at 1.27 mg/mL) and TBARS formation inhibition (50% at 0.78 mg/mL), respectively. The applied processing technologies affected favourably the antioxidant potential of M. procera extracts, particularly dryness for DPPH scavenging activity and β-carotene bleaching inhibition, freezing for reducing power and irradiation for TBARS formation inhibition.
Autores principais:Fernandes, Ângela
Outros Autores:Carocho, Márcio; Antonio, Amilcar L.; Oliveira, Beatriz; Martins, Anabela; Ferreira, Isabel C.F.R.
Assunto:Wild mushroom Macrolepiota procera Processed samples Antioxidant activity
Ano:2013
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Fernandes, Ângela
author2 Carocho, Márcio
Antonio, Amilcar L.
Oliveira, Beatriz
Martins, Anabela
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author_facet Fernandes, Ângela
Carocho, Márcio
Antonio, Amilcar L.
Oliveira, Beatriz
Martins, Anabela
Ferreira, Isabel C.F.R.
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Fernandes, Ângela\",\"Person.identifier.orcid\":\"0000-0002-0157-9873\"},{\"Person.name\":\"Carocho, Márcio\",\"Person.identifier.orcid\":\"0000-0002-8978-4547\"},{\"Person.name\":\"Antonio, Amilcar L.\",\"Person.identifier.orcid\":\"0000-0001-8271-9964\"},{\"Person.name\":\"Oliveira, Beatriz\"},{\"Person.name\":\"Martins, Anabela\",\"Person.identifier.orcid\":\"0000-0001-6218-4413\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Fernandes, Ângela
Carocho, Márcio
Antonio, Amilcar L.
Oliveira, Beatriz
Martins, Anabela
Ferreira, Isabel C.F.R.
datacite.date.Accepted.fl_str_mv 2013-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2013-09-06T14:52:13Z
datacite.date.embargoed.fl_str_mv 2013-09-06T14:52:13Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Wild mushroom
Macrolepiota procera
Processed samples
Antioxidant activity
datacite.titles.title.fl_str_mv Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Fernandes, Ângela
Carocho, Márcio
Antonio, Amilcar L.
Oliveira, Beatriz
Martins, Anabela
Ferreira, Isabel C.F.R.
dc.date.Accepted.fl_str_mv 2013-01-01T00:00:00Z
dc.date.available.fl_str_mv 2013-09-06T14:52:13Z
dc.date.embargoed.fl_str_mv 2013-09-06T14:52:13Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/8656
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Wild mushroom
Macrolepiota procera
Processed samples
Antioxidant activity
dc.title.fl_str_mv Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description Mushrooms are very perishable food products and tend to lose quality immediately after harveStr. Drying is the most common method for preserving mushrooms, freezing is becoming increasingly popular and food irradiation has also been suggested by many researchers as a good conservation technique in order to maintain and increase the food shelf life. In the present work, the effects of different processing technologies (freezing, drying and gamma irradiation) on antioxidant activity of the wild mushroom Macrolepiota procera were evaluated. Fruiting bodies were obtained in Trás-os-Montes, in the Northeast of Portugal, in November 2011. The irradiation was performed in experimental equipment with four 60Co sources at 0.6 kGy. The samples were submitted to different processing technologies: freezing (at -20 ºC in a freezer), drying (at 30 ºC in an oven) and gamma irradiation in fresh samples. Antioxidant activity was determined in the methanolic extracts by in vitro assays measuring DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) assay. Total phenolics were also determined by the Folin-Ciocalteu assay. Dried samples gave the highest DPPH scavenging activity (50% at 2.7 mg/mL), β-carotene bleaching inhibition (50% at 1.10 mg/mL) and the highest phenolic content (19.2 mg GAE/g methanolic extract). Frozen and irradiated samples presented the highest reducing power (0.5 absorbance at 1.27 mg/mL) and TBARS formation inhibition (50% at 0.78 mg/mL), respectively. The applied processing technologies affected favourably the antioxidant potential of M. procera extracts, particularly dryness for DPPH scavenging activity and β-carotene bleaching inhibition, freezing for reducing power and irradiation for TBARS formation inhibition.
dirty 0
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funding.funder.alternateName_str_mv FCT
FCT
funding.funder.identifier_str_mv http://doi.org/10.13039/501100001871
http://doi.org/10.13039/501100001871
funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
Fundação para a Ciência e a Tecnologia
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6820 - DCRRNI ID
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organization_str_mv urn:organizationAcronym:ipb
person_str_mv Fernandes, Ângela
Fernandes, Ângela
https://www.ciencia-id.pt/2015-0434-AE7C
2015-0434-AE7C
http://orcid.org/0000-0002-0157-9873
0000-0002-0157-9873
Carocho, Márcio
Carocho, Márcio
https://www.ciencia-id.pt/5614-005B-5CF1
5614-005B-5CF1
http://orcid.org/0000-0002-8978-4547
0000-0002-8978-4547
Antonio, Amilcar L.
Antonio, Amilcar L.
https://www.ciencia-id.pt/1014-5259-DE86
1014-5259-DE86
http://orcid.org/0000-0001-8271-9964
0000-0001-8271-9964
Oliveira, Beatriz
Martins, Anabela
Martins, Anabela
https://www.ciencia-id.pt/7B18-A810-6B93
7B18-A810-6B93
http://orcid.org/0000-0001-6218-4413
0000-0001-6218-4413
Ferreira, Isabel C.F.R.
Ferreira, Isabel C.F.R.
https://www.ciencia-id.pt/9418-CF95-9919
9418-CF95-9919
http://orcid.org/0000-0003-4910-4882
0000-0003-4910-4882
publishDate 2013
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spelling engporMushrooms are very perishable food products and tend to lose quality immediately after harveStr. Drying is the most common method for preserving mushrooms, freezing is becoming increasingly popular and food irradiation has also been suggested by many researchers as a good conservation technique in order to maintain and increase the food shelf life. In the present work, the effects of different processing technologies (freezing, drying and gamma irradiation) on antioxidant activity of the wild mushroom Macrolepiota procera were evaluated. Fruiting bodies were obtained in Trás-os-Montes, in the Northeast of Portugal, in November 2011. The irradiation was performed in experimental equipment with four 60Co sources at 0.6 kGy. The samples were submitted to different processing technologies: freezing (at -20 ºC in a freezer), drying (at 30 ºC in an oven) and gamma irradiation in fresh samples. Antioxidant activity was determined in the methanolic extracts by in vitro assays measuring DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of lipid peroxidation using thiobarbituric acid reactive substances (TBARS) assay. Total phenolics were also determined by the Folin-Ciocalteu assay. Dried samples gave the highest DPPH scavenging activity (50% at 2.7 mg/mL), β-carotene bleaching inhibition (50% at 1.10 mg/mL) and the highest phenolic content (19.2 mg GAE/g methanolic extract). Frozen and irradiated samples presented the highest reducing power (0.5 absorbance at 1.27 mg/mL) and TBARS formation inhibition (50% at 0.78 mg/mL), respectively. The applied processing technologies affected favourably the antioxidant potential of M. procera extracts, particularly dryness for DPPH scavenging activity and β-carotene bleaching inhibition, freezing for reducing power and irradiation for TBARS formation inhibition.application/pdfporAntioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologiesPersonalFernandes, ÂngelaDSpacehttp://dspace.org/items/6233e657-3885-40a3-885e-0d51cf4dbaadDSpacehttp://dspace.org/items/6233e657-3885-40a3-885e-0d51cf4dbaadFernandesÂngelaCiência IDhttps://www.ciencia-id.pt2015-0434-AE7CORCIDhttp://orcid.org0000-0002-0157-9873PersonalCarocho, MárcioDSpacehttp://dspace.org/items/46b39e1f-2674-4be1-a96a-c2e5792e6979DSpacehttp://dspace.org/items/46b39e1f-2674-4be1-a96a-c2e5792e6979CarochoMárcioCiência IDhttps://www.ciencia-id.pt5614-005B-5CF1ORCIDhttp://orcid.org0000-0002-8978-4547Scopus Author IDhttps://www.scopus.com52463229800PersonalAntonio, Amilcar L.DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28AntonioAmilcar L.Ciência IDhttps://www.ciencia-id.pt1014-5259-DE86ORCIDhttp://orcid.org0000-0001-8271-9964Researcher IDhttps://www.researcherid.comJ-1960-2014Scopus Author IDhttps://www.scopus.com44560921900Oliveira, BeatrizPersonalMartins, AnabelaDSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581DSpacehttp://dspace.org/items/383370dd-10e5-40e6-9a15-f2201fe95581MartinsAnabelaCiência IDhttps://www.ciencia-id.pt7B18-A810-6B93ORCIDhttp://orcid.org0000-0001-6218-4413Researcher IDhttps://www.researcherid.comG-5488-2013Scopus Author IDhttps://www.scopus.com7203013518PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-966-612-141-02013-09-06T14:52:13Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/8656http://purl.org/coar/access_right/c_abf2open accessWild mushroomMacrolepiota proceraProcessed samplesAntioxidant activity2350721 bytesFundação para a Ciência e a TecnologiaStrategic Project - UI 690 - 2011-20126817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaStrategic Project - LA 6 - 2011-20126820 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/5978df72-b39b-476b-b80a-e6381a044c28/downloadThe Second North and East European Congress on Food. NEEFood – 2013. 26-29 May 2013Kyiv, Ukraine
spellingShingle Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
Fernandes, Ângela
Wild mushroom
Macrolepiota procera
Processed samples
Antioxidant activity
status SINGLETON
subject.fl_str_mv Wild mushroom
Macrolepiota procera
Processed samples
Antioxidant activity
title Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
title_full Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
title_fullStr Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
title_full_unstemmed Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
title_short Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
title_sort Antioxidant activity of Macrolepiota procera wild mushroom submitted to different processing technologies
topic Wild mushroom
Macrolepiota procera
Processed samples
Antioxidant activity
topic_facet Wild mushroom
Macrolepiota procera
Processed samples
Antioxidant activity
url http://hdl.handle.net/10198/8656
visible 1