Publicação
Monolith technology in isolation of human immunoglobulin G subclasses
| Resumo: | In the present study, a method was developed for the separation of hIgG into its subclasses with the advantages of monolithic technology. pH elution was applied using Protein A monolith column in a stepwise method and conditions were optimized. Since IgG3 has very low affinity to Protein A, elution was occurred immediately upon injection of the sample. IgG1 and IgG4 were eluted simultaneously under the applied conditions. Furthermore, IgG2 was separated with significant purity. According to the present results, monolithic Protein A column could be proposed as a time efficient separation media for the enrichment of IgG2 which is the most often directed antibody against bacterial pathogens. |
|---|---|
| Autores principais: | Leblebici, Pelin |
| Outros Autores: | Rodrigues, Alírio; Pais, Luís S. |
| Assunto: | Human immunoglobulin G Monolithic stationary phase Protein A |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172755804258304 |
|---|---|
| author | Leblebici, Pelin |
| author2 | Rodrigues, Alírio Pais, Luís S. |
| author2_role | author author |
| author_facet | Leblebici, Pelin Rodrigues, Alírio Pais, Luís S. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Leblebici, Pelin\"},{\"Person.name\":\"Rodrigues, Alírio\"},{\"Person.name\":\"Pais, Luís S.\",\"Person.identifier.orcid\":\"0000-0002-3000-2862\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Leblebici, Pelin Rodrigues, Alírio Pais, Luís S. |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-01-14T14:12:07Z |
| datacite.date.embargoed.fl_str_mv | 2014-01-14T14:12:07Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Human immunoglobulin G Monolithic stationary phase Protein A |
| datacite.titles.title.fl_str_mv | Monolith technology in isolation of human immunoglobulin G subclasses |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Leblebici, Pelin Rodrigues, Alírio Pais, Luís S. |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-01-14T14:12:07Z |
| dc.date.embargoed.fl_str_mv | 2014-01-14T14:12:07Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/9151 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Human immunoglobulin G Monolithic stationary phase Protein A |
| dc.title.fl_str_mv | Monolith technology in isolation of human immunoglobulin G subclasses |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | In the present study, a method was developed for the separation of hIgG into its subclasses with the advantages of monolithic technology. pH elution was applied using Protein A monolith column in a stepwise method and conditions were optimized. Since IgG3 has very low affinity to Protein A, elution was occurred immediately upon injection of the sample. IgG1 and IgG4 were eluted simultaneously under the applied conditions. Furthermore, IgG2 was separated with significant purity. According to the present results, monolithic Protein A column could be proposed as a time efficient separation media for the enrichment of IgG2 which is the most often directed antibody against bacterial pathogens. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/a2f43b46-6cd0-422a-9239-0128a3d095f7/download |
| id | ipb_ab7005dbe2ccfc167bde59c7774e7abd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/9151 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/9151 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Leblebici, Pelin Rodrigues, Alírio Pais, Luís S. Pais, Luís S. https://www.ciencia-id.pt/421E-0CCD-A84F 421E-0CCD-A84F http://orcid.org/0000-0002-3000-2862 0000-0002-3000-2862 |
| publishDate | 2013 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engporIn the present study, a method was developed for the separation of hIgG into its subclasses with the advantages of monolithic technology. pH elution was applied using Protein A monolith column in a stepwise method and conditions were optimized. Since IgG3 has very low affinity to Protein A, elution was occurred immediately upon injection of the sample. IgG1 and IgG4 were eluted simultaneously under the applied conditions. Furthermore, IgG2 was separated with significant purity. According to the present results, monolithic Protein A column could be proposed as a time efficient separation media for the enrichment of IgG2 which is the most often directed antibody against bacterial pathogens.application/pdfporMonolith technology in isolation of human immunoglobulin G subclassesLeblebici, PelinRodrigues, AlírioPersonalPais, Luís S.DSpacehttp://dspace.org/items/c6d25534-8487-45b1-90af-d350f4e3ac55DSpacehttp://dspace.org/items/c6d25534-8487-45b1-90af-d350f4e3ac55PaisLuís S.Ciência IDhttps://www.ciencia-id.pt421E-0CCD-A84FORCIDhttp://orcid.org0000-0002-3000-2862Scopus Author IDhttps://www.scopus.com6603930478HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2014-01-14T14:12:07Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/9151http://purl.org/coar/access_right/c_abf2open accessHuman immunoglobulin GMonolithic stationary phaseProtein A380325 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/a2f43b46-6cd0-422a-9239-0128a3d095f7/downloadAIChE Annual MeetingSan Francisco, CA, USA |
| spellingShingle | Monolith technology in isolation of human immunoglobulin G subclasses Leblebici, Pelin Human immunoglobulin G Monolithic stationary phase Protein A |
| status | SINGLETON |
| subject.fl_str_mv | Human immunoglobulin G Monolithic stationary phase Protein A |
| title | Monolith technology in isolation of human immunoglobulin G subclasses |
| title_full | Monolith technology in isolation of human immunoglobulin G subclasses |
| title_fullStr | Monolith technology in isolation of human immunoglobulin G subclasses |
| title_full_unstemmed | Monolith technology in isolation of human immunoglobulin G subclasses |
| title_short | Monolith technology in isolation of human immunoglobulin G subclasses |
| title_sort | Monolith technology in isolation of human immunoglobulin G subclasses |
| topic | Human immunoglobulin G Monolithic stationary phase Protein A |
| topic_facet | Human immunoglobulin G Monolithic stationary phase Protein A |
| url | http://hdl.handle.net/10198/9151 |
| visible | 1 |