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Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability
| Resumo: | Phenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) and processing conditions (concentration and lyophilization) was also investigated. The results showed that ultrasound-assisted extraction (UAE) provided the highest yield of total phenolic compounds (74.89 mg GAE/g), of which 71.23% were represented by total anthocyanins and 62.50% by monomeric anthocyanins, with total flavonoids of 8.11–9.41 mg RUE/g. The analysis of individual phenolic compounds reconfirms the efficiency of UAE, obtaining 0.42–0.09 mg/g gallic acid, 0.59–0.01 mg/g chlorogenic acid, 0.17–0.03 mg/g 4-coumaric acid, and 2.43–0.01 mg/g rutin. The optimization of the extraction conditions led to the conclusion that the best solvent is 45% ethanol (v/v), and the optimal parameters are 40 ◦C for 40 min. It was also found that the stability of the extracts can be high during long periods of time (even after 180 days). These findings contribute to the understanding of the optimization of extraction processes and storage conditions to obtain extracts rich in phenolic compounds from elderberries, with potential uses in pharmaceutical andb food applications. |
|---|---|
| Autores principais: | Pascariu, Oana-Elena |
| Outros Autores: | Dias, L.G.; Israel-Roming, Florentina |
| Assunto: | Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| Ano: | 2024 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173345309491200 |
|---|---|
| author | Pascariu, Oana-Elena |
| author2 | Dias, L.G. Israel-Roming, Florentina |
| author2_role | author author |
| author_facet | Pascariu, Oana-Elena Dias, L.G. Israel-Roming, Florentina |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pascariu, Oana-Elena\"},{\"Person.name\":\"Dias, L.G.\",\"Person.identifier.orcid\":\"0000-0002-1210-4259\"},{\"Person.name\":\"Israel-Roming, Florentina\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pascariu, Oana-Elena Dias, L.G. Israel-Roming, Florentina |
| datacite.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2024-10-08T11:23:19Z |
| datacite.date.embargoed.fl_str_mv | 2024-10-08T11:23:19Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| datacite.titles.title.fl_str_mv | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pascariu, Oana-Elena Dias, L.G. Israel-Roming, Florentina |
| dc.date.Accepted.fl_str_mv | 2024-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2024-10-08T11:23:19Z |
| dc.date.embargoed.fl_str_mv | 2024-10-08T11:23:19Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/30365 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| dc.title.fl_str_mv | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Phenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) and processing conditions (concentration and lyophilization) was also investigated. The results showed that ultrasound-assisted extraction (UAE) provided the highest yield of total phenolic compounds (74.89 mg GAE/g), of which 71.23% were represented by total anthocyanins and 62.50% by monomeric anthocyanins, with total flavonoids of 8.11–9.41 mg RUE/g. The analysis of individual phenolic compounds reconfirms the efficiency of UAE, obtaining 0.42–0.09 mg/g gallic acid, 0.59–0.01 mg/g chlorogenic acid, 0.17–0.03 mg/g 4-coumaric acid, and 2.43–0.01 mg/g rutin. The optimization of the extraction conditions led to the conclusion that the best solvent is 45% ethanol (v/v), and the optimal parameters are 40 ◦C for 40 min. It was also found that the stability of the extracts can be high during long periods of time (even after 180 days). These findings contribute to the understanding of the optimization of extraction processes and storage conditions to obtain extracts rich in phenolic compounds from elderberries, with potential uses in pharmaceutical andb food applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/1a1deb96-a34a-40ef-b6fd-a26fc5fd7824/download |
| id | ipb_b2c5ca7b45fdc09cc09d2a03176cfe52 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/30365 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/30365 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pascariu, Oana-Elena Dias, L.G. Dias, L.G. https://www.ciencia-id.pt/2F11-9092-FAAF 2F11-9092-FAAF http://orcid.org/0000-0002-1210-4259 0000-0002-1210-4259 Israel-Roming, Florentina |
| publishDate | 2024 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engMDPIenPhenolic compounds from elderberries (Sambucus nigra L.) have attracted attention due to their potential health benefits. This paper examines different extraction methods used to obtain phenolic compounds from these fruits and the stability of the extracts. Several extraction techniques (extraction with continuous agitation, ultrasound-assisted extraction, microwave-assisted extraction, maceration, and enzyme-assisted extraction) were tested and compared to evaluate the yield and the quality of the extracts. The stability of the extracts with various storage parameters (time and temperature) and processing conditions (concentration and lyophilization) was also investigated. The results showed that ultrasound-assisted extraction (UAE) provided the highest yield of total phenolic compounds (74.89 mg GAE/g), of which 71.23% were represented by total anthocyanins and 62.50% by monomeric anthocyanins, with total flavonoids of 8.11–9.41 mg RUE/g. The analysis of individual phenolic compounds reconfirms the efficiency of UAE, obtaining 0.42–0.09 mg/g gallic acid, 0.59–0.01 mg/g chlorogenic acid, 0.17–0.03 mg/g 4-coumaric acid, and 2.43–0.01 mg/g rutin. The optimization of the extraction conditions led to the conclusion that the best solvent is 45% ethanol (v/v), and the optimal parameters are 40 ◦C for 40 min. It was also found that the stability of the extracts can be high during long periods of time (even after 180 days). These findings contribute to the understanding of the optimization of extraction processes and storage conditions to obtain extracts rich in phenolic compounds from elderberries, with potential uses in pharmaceutical andb food applications.application/pdfpt_PTOptimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract StabilityPascariu, Oana-ElenaPersonalDias, L.G.DSpacehttp://dspace.org/items/eac8c166-4056-4ed0-8d8d-7ecb2c4481a5DSpacehttp://dspace.org/items/eac8c166-4056-4ed0-8d8d-7ecb2c4481a5DiasLuís G.Ciência IDhttps://www.ciencia-id.pt2F11-9092-FAAFORCIDhttp://orcid.org0000-0002-1210-4259Scopus Author IDhttps://www.scopus.com23569169900Israel-Roming, FlorentinaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2311-7524DOIIsPartOf10.3390/horticulturae100707432024-10-08T11:23:19Z20242024-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/30365http://purl.org/coar/access_right/c_abf2open accessSambucus nigraBioactive compoundsExtractionPhenolic compounds3690488 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2024http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/1a1deb96-a34a-40ef-b6fd-a26fc5fd7824/downloadHorticulturae107743 |
| spellingShingle | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability Pascariu, Oana-Elena Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| status | SINGLETON |
| subject.fl_str_mv | Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| title | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| title_full | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| title_fullStr | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| title_full_unstemmed | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| title_short | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| title_sort | Optimization of Extraction Method of Bioactive Compounds from Elderberries (Sambucus nigra L.) and Testing Extract Stability |
| topic | Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| topic_facet | Sambucus nigra Bioactive compounds Extraction Phenolic compounds |
| url | http://hdl.handle.net/10198/30365 |
| visible | 1 |