Publicação
Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter
| Resumo: | To better understand the prevalence of Feline Immunodeficiency Virus and Feline Leukemia Virus in cat shelters, an epidemiological study was conducted in an intermunicipal shelter in the Northeast Portugal. MATERIAL AND METHODS: Samples were collected as part of routine plan for control and monitoring of infectious diseases in cats at lntermunicipal Official Shelter. This shelter houses stray cats from the five municipalities of Terra Quente Transmontana, which has permanent veterinary medical and nursing assistance with daily exams, identification, registration, vaccination, deworming and neutering procedures. Blood samples were collected during periodic checks carried out by the veterinarian in charge of the shelter, from May 2017 to January 2021. A total of 1 71 samples were collected from stray cats housed at this shelter, that serves: Alf§.ndega da Fe, Carrazeda de Ansiaes, Macedo de Cavaleiros, Mirandela and Vila Flor. A blood sample was collected individually from each cat during medical routine check-ups. Blood was collected from the cephalic vein. To diagnose FIV and FeLV, a kit based on immunochromatographic technique (Uranotest® FeLVFIV, Urano®vet, S.L, Barcelona, Spain) was used in accordance with manufacturer's instructions. This test allows simultaneously qualitative detection of FIV antibody (gp40) and FeLV antigen (p27) in feline whole blood, serum, and plasma. The Uranotest® FeLV-FIV reported a sensitivity of 94% versus viral isolation and specificity of 99% versus viral isolation, for Fe LV, and a sensitivity of 96% versus Western Blot and a specificity of 98% versus Western Blot, for FIV. RESULTS: A total of 171 stray cats in the lntermunicipal Official Shelter were tested during the study period. The sample consisted of 102 females (60%) and 69 males (40%). The overall prevalence of FIV and FeLV was 13.45% (23/171, 95% Cl: 9.13-19.37%) of the cats tested. The proportion of cats positive to FIV, FeLV and to both FIV and FeLV was 9.36% (16/171, 95% Cl: 5.84-14.66%), 1.75% (3/171, 95% Cl: 0.60-5.03%) and 2.34% (41171, 95% Cl: 0.91-5.86%), respectively. CONCLUSION: The results indicated the overall seroprevalence of FIV and FeLV in stray cats entering the lntermunicipal Official Shelter was very high and risk factors associated to seroprevalence need to be study in further studies. |
|---|---|
| Autores principais: | Afonso, Paulo |
| Outros Autores: | Quintas, Helder; Cardoso, Luís Miguel; Coelho, Ana Cláudia |
| Assunto: | Feline Feline leukemia |
| Ano: | 2021 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172854853795840 |
|---|---|
| author | Afonso, Paulo |
| author2 | Quintas, Helder Cardoso, Luís Miguel Coelho, Ana Cláudia |
| author2_role | author author author |
| author_facet | Afonso, Paulo Quintas, Helder Cardoso, Luís Miguel Coelho, Ana Cláudia |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Afonso, Paulo\",\"Person.identifier.orcid\":\"0000-0001-6390-0194\"},{\"Person.name\":\"Quintas, Helder\",\"Person.identifier.orcid\":\"0000-0002-6934-1669\"},{\"Person.name\":\"Cardoso, Luís Miguel\"},{\"Person.name\":\"Coelho, Ana Cláudia\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Afonso, Paulo Quintas, Helder Cardoso, Luís Miguel Coelho, Ana Cláudia |
| datacite.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2022-01-20T11:49:57Z |
| datacite.date.embargoed.fl_str_mv | 2022-01-20T11:49:57Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Feline Feline leukemia |
| datacite.titles.title.fl_str_mv | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Afonso, Paulo Quintas, Helder Cardoso, Luís Miguel Coelho, Ana Cláudia |
| dc.date.Accepted.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2022-01-20T11:49:57Z |
| dc.date.embargoed.fl_str_mv | 2022-01-20T11:49:57Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/24792 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Feline Feline leukemia |
| dc.title.fl_str_mv | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | To better understand the prevalence of Feline Immunodeficiency Virus and Feline Leukemia Virus in cat shelters, an epidemiological study was conducted in an intermunicipal shelter in the Northeast Portugal. MATERIAL AND METHODS: Samples were collected as part of routine plan for control and monitoring of infectious diseases in cats at lntermunicipal Official Shelter. This shelter houses stray cats from the five municipalities of Terra Quente Transmontana, which has permanent veterinary medical and nursing assistance with daily exams, identification, registration, vaccination, deworming and neutering procedures. Blood samples were collected during periodic checks carried out by the veterinarian in charge of the shelter, from May 2017 to January 2021. A total of 1 71 samples were collected from stray cats housed at this shelter, that serves: Alf§.ndega da Fe, Carrazeda de Ansiaes, Macedo de Cavaleiros, Mirandela and Vila Flor. A blood sample was collected individually from each cat during medical routine check-ups. Blood was collected from the cephalic vein. To diagnose FIV and FeLV, a kit based on immunochromatographic technique (Uranotest® FeLVFIV, Urano®vet, S.L, Barcelona, Spain) was used in accordance with manufacturer's instructions. This test allows simultaneously qualitative detection of FIV antibody (gp40) and FeLV antigen (p27) in feline whole blood, serum, and plasma. The Uranotest® FeLV-FIV reported a sensitivity of 94% versus viral isolation and specificity of 99% versus viral isolation, for Fe LV, and a sensitivity of 96% versus Western Blot and a specificity of 98% versus Western Blot, for FIV. RESULTS: A total of 171 stray cats in the lntermunicipal Official Shelter were tested during the study period. The sample consisted of 102 females (60%) and 69 males (40%). The overall prevalence of FIV and FeLV was 13.45% (23/171, 95% Cl: 9.13-19.37%) of the cats tested. The proportion of cats positive to FIV, FeLV and to both FIV and FeLV was 9.36% (16/171, 95% Cl: 5.84-14.66%), 1.75% (3/171, 95% Cl: 0.60-5.03%) and 2.34% (41171, 95% Cl: 0.91-5.86%), respectively. CONCLUSION: The results indicated the overall seroprevalence of FIV and FeLV in stray cats entering the lntermunicipal Official Shelter was very high and risk factors associated to seroprevalence need to be study in further studies. |
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| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/c457eb99-0049-4e4d-8409-e14ea0862893/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/24792 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/24792 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Afonso, Paulo Afonso, Paulo https://www.ciencia-id.pt/FE18-A3F5-6996 FE18-A3F5-6996 http://orcid.org/0000-0001-6390-0194 0000-0001-6390-0194 Quintas, Helder Quintas, Helder https://www.ciencia-id.pt/6018-8BBD-80AB 6018-8BBD-80AB http://orcid.org/0000-0002-6934-1669 0000-0002-6934-1669 Cardoso, Luís Miguel Coelho, Ana Cláudia |
| publishDate | 2021 |
| reponame_str | Biblioteca Digital do IPB |
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| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTTo better understand the prevalence of Feline Immunodeficiency Virus and Feline Leukemia Virus in cat shelters, an epidemiological study was conducted in an intermunicipal shelter in the Northeast Portugal. MATERIAL AND METHODS: Samples were collected as part of routine plan for control and monitoring of infectious diseases in cats at lntermunicipal Official Shelter. This shelter houses stray cats from the five municipalities of Terra Quente Transmontana, which has permanent veterinary medical and nursing assistance with daily exams, identification, registration, vaccination, deworming and neutering procedures. Blood samples were collected during periodic checks carried out by the veterinarian in charge of the shelter, from May 2017 to January 2021. A total of 1 71 samples were collected from stray cats housed at this shelter, that serves: Alf§.ndega da Fe, Carrazeda de Ansiaes, Macedo de Cavaleiros, Mirandela and Vila Flor. A blood sample was collected individually from each cat during medical routine check-ups. Blood was collected from the cephalic vein. To diagnose FIV and FeLV, a kit based on immunochromatographic technique (Uranotest® FeLVFIV, Urano®vet, S.L, Barcelona, Spain) was used in accordance with manufacturer's instructions. This test allows simultaneously qualitative detection of FIV antibody (gp40) and FeLV antigen (p27) in feline whole blood, serum, and plasma. The Uranotest® FeLV-FIV reported a sensitivity of 94% versus viral isolation and specificity of 99% versus viral isolation, for Fe LV, and a sensitivity of 96% versus Western Blot and a specificity of 98% versus Western Blot, for FIV. RESULTS: A total of 171 stray cats in the lntermunicipal Official Shelter were tested during the study period. The sample consisted of 102 females (60%) and 69 males (40%). The overall prevalence of FIV and FeLV was 13.45% (23/171, 95% Cl: 9.13-19.37%) of the cats tested. The proportion of cats positive to FIV, FeLV and to both FIV and FeLV was 9.36% (16/171, 95% Cl: 5.84-14.66%), 1.75% (3/171, 95% Cl: 0.60-5.03%) and 2.34% (41171, 95% Cl: 0.91-5.86%), respectively. CONCLUSION: The results indicated the overall seroprevalence of FIV and FeLV in stray cats entering the lntermunicipal Official Shelter was very high and risk factors associated to seroprevalence need to be study in further studies.application/pdfpt_PTSeroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelterPersonalAfonso, PauloDSpacehttp://dspace.org/items/96598273-5f15-429d-b3fd-72304d20e2d6DSpacehttp://dspace.org/items/96598273-5f15-429d-b3fd-72304d20e2d6AfonsoPauloCiência IDhttps://www.ciencia-id.ptFE18-A3F5-6996ORCIDhttp://orcid.org0000-0001-6390-0194PersonalQuintas, HelderDSpacehttp://dspace.org/items/068e74bc-656d-4fd1-93ed-f7346baa3b69DSpacehttp://dspace.org/items/068e74bc-656d-4fd1-93ed-f7346baa3b69QuintasHelderCiência IDhttps://www.ciencia-id.pt6018-8BBD-80ABORCIDhttp://orcid.org0000-0002-6934-1669Scopus Author IDhttps://www.scopus.com35796342900Cardoso, Luís MiguelCoelho, Ana CláudiaHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2022-01-20T11:49:57Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/24792http://purl.org/coar/access_right/c_abf2open accessFelineFeline leukemia1328512 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/c457eb99-0049-4e4d-8409-e14ea0862893/download3RD International Conference of the European College of Veterinary Microbiology |
| spellingShingle | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter Afonso, Paulo Feline Feline leukemia |
| status | SINGLETON |
| subject.fl_str_mv | Feline Feline leukemia |
| title | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| title_full | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| title_fullStr | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| title_full_unstemmed | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| title_short | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| title_sort | Seroprevalence of feline immunodeficiency vírus and feline leucemia vírus in a portuguese intermunicipal shelter |
| topic | Feline Feline leukemia |
| topic_facet | Feline Feline leukemia |
| url | http://hdl.handle.net/10198/24792 |
| visible | 1 |