Publicação
Bacterial dynamics and exchange in plant-insect interactions
| Resumo: | In nature, plants and insects engage in intricate interactions. Despite the increasing knowledge of the microbiomes of plants and insects, the extent to which they exchange and alter each other’s microbiomes remains unclear. In this work, the bacterial community associated with nymphs of Philaenus spumarius (Hemiptera: Aphrophoridae), the stems of Coleostephus myconis where the nymphs were feeding, and the foam produced by the nymphs, were studied by culture-dependent and -independent approaches, with an attempt to elucidate the exchange of bacteria between plants and insects. The results suggest that both approaches complement each other, as many bacterial genera identified by metabarcoding were not detected by culturing, and vice versa. Overall, stems and foam exhibited higher bacterial diversity than nymphs, with all the samples showing enrichment in bacteria known to provide diverse benefits to their host. Stems and foam were the most similar in bacterial composition, but Burkholderiaceae and Moraxellaceae dominated the stems, whereas Rhizobiaceae and Sphingobacteriaceae dominated the foam. Nymphs exhibit the most distinct bacterial composition, yet more similar to that found in the stem compared to the foam. Indeed, nymphs were enriched on endosymbiotic bacteria, mostly Candidatus Sulcia and Sodalis, not found in the stem and foam. Nevertheless, during feeding, nymphs appeared to exchange several bacteria genera with C. myconis, with a significant number being incor- porated into the bacteriome of the nymph. The genera Curvibacter, Cutibacterium, Methylobacterium, Pseudomonas and Rhizobium are likely the most exchanged. Nymphs also appear to exchange bacteria to the foam, notably species from the Enhydrobacter, Pseudomonas, Rhizobium and Roseomonas genera. More studies to infer the functions of the shared bacteria between P. spumarius-C. myconis are needed. |
|---|---|
| Autores principais: | Cameirão, Cristina |
| Outros Autores: | Pereira, José Alberto; Tavares, Rui; Lino-Neto, Teresa; Baptista, Paula |
| Assunto: | Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173271349231616 |
|---|---|
| author | Cameirão, Cristina |
| author2 | Pereira, José Alberto Tavares, Rui Lino-Neto, Teresa Baptista, Paula |
| author2_role | author author author author |
| author_facet | Cameirão, Cristina Pereira, José Alberto Tavares, Rui Lino-Neto, Teresa Baptista, Paula |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Cameirão, Cristina\",\"Person.identifier.orcid\":\"0000-0001-5745-9822\"},{\"Person.name\":\"Pereira, José Alberto\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\"},{\"Person.name\":\"Tavares, Rui\"},{\"Person.name\":\"Lino-Neto, Teresa\"},{\"Person.name\":\"Baptista, Paula\",\"Person.identifier.orcid\":\"0000-0001-6331-3731\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Cameirão, Cristina Pereira, José Alberto Tavares, Rui Lino-Neto, Teresa Baptista, Paula |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2025-04-23T08:27:39Z |
| datacite.date.embargoed.fl_str_mv | 2025-04-23T08:27:39Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| datacite.titles.title.fl_str_mv | Bacterial dynamics and exchange in plant-insect interactions |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Cameirão, Cristina Pereira, José Alberto Tavares, Rui Lino-Neto, Teresa Baptista, Paula |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2025-04-23T08:27:39Z |
| dc.date.embargoed.fl_str_mv | 2025-04-23T08:27:39Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/34424 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| dc.title.fl_str_mv | Bacterial dynamics and exchange in plant-insect interactions |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In nature, plants and insects engage in intricate interactions. Despite the increasing knowledge of the microbiomes of plants and insects, the extent to which they exchange and alter each other’s microbiomes remains unclear. In this work, the bacterial community associated with nymphs of Philaenus spumarius (Hemiptera: Aphrophoridae), the stems of Coleostephus myconis where the nymphs were feeding, and the foam produced by the nymphs, were studied by culture-dependent and -independent approaches, with an attempt to elucidate the exchange of bacteria between plants and insects. The results suggest that both approaches complement each other, as many bacterial genera identified by metabarcoding were not detected by culturing, and vice versa. Overall, stems and foam exhibited higher bacterial diversity than nymphs, with all the samples showing enrichment in bacteria known to provide diverse benefits to their host. Stems and foam were the most similar in bacterial composition, but Burkholderiaceae and Moraxellaceae dominated the stems, whereas Rhizobiaceae and Sphingobacteriaceae dominated the foam. Nymphs exhibit the most distinct bacterial composition, yet more similar to that found in the stem compared to the foam. Indeed, nymphs were enriched on endosymbiotic bacteria, mostly Candidatus Sulcia and Sodalis, not found in the stem and foam. Nevertheless, during feeding, nymphs appeared to exchange several bacteria genera with C. myconis, with a significant number being incor- porated into the bacteriome of the nymph. The genera Curvibacter, Cutibacterium, Methylobacterium, Pseudomonas and Rhizobium are likely the most exchanged. Nymphs also appear to exchange bacteria to the foam, notably species from the Enhydrobacter, Pseudomonas, Rhizobium and Roseomonas genera. More studies to infer the functions of the shared bacteria between P. spumarius-C. myconis are needed. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/95a0299e-e723-4cff-9cba-abbf30acc723/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID 6817 - DCRRNI ID |
| id | ipb_b8935bd424f8eba2976d07dcb1edbc1d |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/34424 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/34424 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Cameirão, Cristina Cameirão, Cristina https://www.ciencia-id.pt/9511-984B-7350 9511-984B-7350 http://orcid.org/0000-0001-5745-9822 0000-0001-5745-9822 Pereira, José Alberto Pereira, José Alberto https://www.ciencia-id.pt/611F-80B2-A7C1 611F-80B2-A7C1 http://orcid.org/0000-0002-2260-0600 0000-0002-2260-0600 Tavares, Rui Lino-Neto, Teresa Baptista, Paula Baptista, Paula https://www.ciencia-id.pt/7D11-FE1E-CD0F 7D11-FE1E-CD0F http://orcid.org/0000-0001-6331-3731 0000-0001-6331-3731 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engElsevierengIn nature, plants and insects engage in intricate interactions. Despite the increasing knowledge of the microbiomes of plants and insects, the extent to which they exchange and alter each other’s microbiomes remains unclear. In this work, the bacterial community associated with nymphs of Philaenus spumarius (Hemiptera: Aphrophoridae), the stems of Coleostephus myconis where the nymphs were feeding, and the foam produced by the nymphs, were studied by culture-dependent and -independent approaches, with an attempt to elucidate the exchange of bacteria between plants and insects. The results suggest that both approaches complement each other, as many bacterial genera identified by metabarcoding were not detected by culturing, and vice versa. Overall, stems and foam exhibited higher bacterial diversity than nymphs, with all the samples showing enrichment in bacteria known to provide diverse benefits to their host. Stems and foam were the most similar in bacterial composition, but Burkholderiaceae and Moraxellaceae dominated the stems, whereas Rhizobiaceae and Sphingobacteriaceae dominated the foam. Nymphs exhibit the most distinct bacterial composition, yet more similar to that found in the stem compared to the foam. Indeed, nymphs were enriched on endosymbiotic bacteria, mostly Candidatus Sulcia and Sodalis, not found in the stem and foam. Nevertheless, during feeding, nymphs appeared to exchange several bacteria genera with C. myconis, with a significant number being incor- porated into the bacteriome of the nymph. The genera Curvibacter, Cutibacterium, Methylobacterium, Pseudomonas and Rhizobium are likely the most exchanged. Nymphs also appear to exchange bacteria to the foam, notably species from the Enhydrobacter, Pseudomonas, Rhizobium and Roseomonas genera. More studies to infer the functions of the shared bacteria between P. spumarius-C. myconis are needed.application/pdfengBacterial dynamics and exchange in plant-insect interactionsPersonalCameirão, CristinaDSpacehttp://dspace.org/items/c7e28508-95c5-4441-a2c3-91d69059016dDSpacehttp://dspace.org/items/c7e28508-95c5-4441-a2c3-91d69059016dCameirãoCristinaCiência IDhttps://www.ciencia-id.pt9511-984B-7350ORCIDhttp://orcid.org0000-0001-5745-9822PersonalPereira, José AlbertoDSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348Tavares, RuiLino-Neto, TeresaPersonalBaptista, PaulaDSpacehttp://dspace.org/items/3f35226a-b17a-4f7d-8da1-3297105cbfe9DSpacehttp://dspace.org/items/3f35226a-b17a-4f7d-8da1-3297105cbfe9BaptistaPaulaCiência IDhttps://www.ciencia-id.pt7D11-FE1E-CD0FORCIDhttp://orcid.org0000-0001-6331-3731Scopus Author IDhttps://www.scopus.com14051688000Scopus Author IDhttps://www.scopus.com57394839600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2666-5158DOIIsPartOf10.1016/j.cris.2025.1001102025-04-23T08:27:39Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/34424http://purl.org/coar/access_right/c_16ecrestricted accessPhilaenus spumariusColeostephus myconisMicrobiome exchangeSymbiotic bacteriaFoam microbiota2569053 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regions6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNovel and sustainable strategies to prevent and mitigate Xylella fastidiosa disease impact on olive treeCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/95a0299e-e723-4cff-9cba-abbf30acc723/downloadCurrent Research in Insect Science7111 |
| spellingShingle | Bacterial dynamics and exchange in plant-insect interactions Cameirão, Cristina Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| status | SINGLETON |
| subject.fl_str_mv | Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| title | Bacterial dynamics and exchange in plant-insect interactions |
| title_full | Bacterial dynamics and exchange in plant-insect interactions |
| title_fullStr | Bacterial dynamics and exchange in plant-insect interactions |
| title_full_unstemmed | Bacterial dynamics and exchange in plant-insect interactions |
| title_short | Bacterial dynamics and exchange in plant-insect interactions |
| title_sort | Bacterial dynamics and exchange in plant-insect interactions |
| topic | Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| topic_facet | Philaenus spumarius Coleostephus myconis Microbiome exchange Symbiotic bacteria Foam microbiota |
| url | http://hdl.handle.net/10198/34424 |
| visible | 1 |