Publicação
A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel
| Resumo: | The loss of the red blood cells (RBCs) deformability is related with many human diseases, such as malaria, hereditary spherocytosis, sickle cell disease, or renal diseases. Hence, during the last years, a variety of technologies have been proposed to gain insights into the factors a ecting the RBCs deformability and their possible direct association with several blood pathologies. In this work, we present a simple microfluidic tool that provides the assessment of motions and deformations of RBCs of end-stage kidney disease (ESKD) patients, under a well-controlled microenvironment. All of the flow studies were performed within a hyperbolic converging microchannels where single-cell deformability was assessed under a controlled homogeneous extensional flow field. By using a passive microfluidic device, RBCs passing through a hyperbolic-shaped contraction were measured by a high-speed video microscopy system, and the velocities and deformability ratios (DR) calculated. Blood samples from 27 individuals, including seven healthy controls and 20 having ESKD with or without diabetes, were analysed. The obtained data indicates that the proposed device is able to detect changes in DR of the RBCs, allowing for distinguishing the samples from the healthy controls and the patients. Overall, the deformability of ESKD patients with and without diabetes type II is lower in comparison with the RBCs from the healthy controls, with this di erence being more evident for the group of ESKD patients with diabetes. RBCs from ESKD patients without diabetes elongate on average 8% less, within the hyperbolic contraction, as compared to healthy controls; whereas, RBCs from ESKD patients with diabetes elongate on average 14% less than the healthy controls. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to assess blood cells deformability due to the huge progress in image processing and high-speed microvisualization technology. |
|---|---|
| Autores principais: | Faustino, Vera |
| Outros Autores: | Rodrigues, Raquel Oliveira; Pinho, Diana; Costa, Elísio; Santos-Silva, Alice; Miranda, Vasco; Amaral, Joana S.; Lima, Rui A. |
| Assunto: | Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| Ano: | 2019 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850679487954944 |
|---|---|
| author | Faustino, Vera |
| author2 | Rodrigues, Raquel Oliveira Pinho, Diana Costa, Elísio Santos-Silva, Alice Miranda, Vasco Amaral, Joana S. Lima, Rui A. |
| author2_role | author author author author author author author |
| author_facet | Faustino, Vera Rodrigues, Raquel Oliveira Pinho, Diana Costa, Elísio Santos-Silva, Alice Miranda, Vasco Amaral, Joana S. Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Faustino, Vera\"},{\"Person.name\":\"Rodrigues, Raquel Oliveira\"},{\"Person.name\":\"Pinho, Diana\",\"Person.identifier.orcid\":\"0000-0002-3884-6496\"},{\"Person.name\":\"Costa, Elísio\"},{\"Person.name\":\"Santos-Silva, Alice\"},{\"Person.name\":\"Miranda, Vasco\"},{\"Person.name\":\"Amaral, Joana S.\",\"Person.identifier.orcid\":\"0000-0002-3648-7303\"},{\"Person.name\":\"Lima, Rui A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Faustino, Vera Rodrigues, Raquel Oliveira Pinho, Diana Costa, Elísio Santos-Silva, Alice Miranda, Vasco Amaral, Joana S. Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| datacite.titles.title.fl_str_mv | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Faustino, Vera Rodrigues, Raquel Oliveira Pinho, Diana Costa, Elísio Santos-Silva, Alice Miranda, Vasco Amaral, Joana S. Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2019-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/20250 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| dc.title.fl_str_mv | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The loss of the red blood cells (RBCs) deformability is related with many human diseases, such as malaria, hereditary spherocytosis, sickle cell disease, or renal diseases. Hence, during the last years, a variety of technologies have been proposed to gain insights into the factors a ecting the RBCs deformability and their possible direct association with several blood pathologies. In this work, we present a simple microfluidic tool that provides the assessment of motions and deformations of RBCs of end-stage kidney disease (ESKD) patients, under a well-controlled microenvironment. All of the flow studies were performed within a hyperbolic converging microchannels where single-cell deformability was assessed under a controlled homogeneous extensional flow field. By using a passive microfluidic device, RBCs passing through a hyperbolic-shaped contraction were measured by a high-speed video microscopy system, and the velocities and deformability ratios (DR) calculated. Blood samples from 27 individuals, including seven healthy controls and 20 having ESKD with or without diabetes, were analysed. The obtained data indicates that the proposed device is able to detect changes in DR of the RBCs, allowing for distinguishing the samples from the healthy controls and the patients. Overall, the deformability of ESKD patients with and without diabetes type II is lower in comparison with the RBCs from the healthy controls, with this di erence being more evident for the group of ESKD patients with diabetes. RBCs from ESKD patients without diabetes elongate on average 8% less, within the hyperbolic contraction, as compared to healthy controls; whereas, RBCs from ESKD patients with diabetes elongate on average 14% less than the healthy controls. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to assess blood cells deformability due to the huge progress in image processing and high-speed microvisualization technology. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/858c87e2-2719-4adc-be46-1a7b1e6734e7/download |
| id | ipb_b9fc2a0dbbe78955c3e2a67f35c91885 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/20250 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/20250 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Faustino, Vera Rodrigues, Raquel Oliveira Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Costa, Elísio Santos-Silva, Alice Miranda, Vasco Amaral, Joana S. Amaral, Joana S. https://www.ciencia-id.pt/5319-7DE8-BEDA 5319-7DE8-BEDA http://orcid.org/0000-0002-3648-7303 0000-0002-3648-7303 Lima, Rui A. |
| publishDate | 2019 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engThe loss of the red blood cells (RBCs) deformability is related with many human diseases, such as malaria, hereditary spherocytosis, sickle cell disease, or renal diseases. Hence, during the last years, a variety of technologies have been proposed to gain insights into the factors a ecting the RBCs deformability and their possible direct association with several blood pathologies. In this work, we present a simple microfluidic tool that provides the assessment of motions and deformations of RBCs of end-stage kidney disease (ESKD) patients, under a well-controlled microenvironment. All of the flow studies were performed within a hyperbolic converging microchannels where single-cell deformability was assessed under a controlled homogeneous extensional flow field. By using a passive microfluidic device, RBCs passing through a hyperbolic-shaped contraction were measured by a high-speed video microscopy system, and the velocities and deformability ratios (DR) calculated. Blood samples from 27 individuals, including seven healthy controls and 20 having ESKD with or without diabetes, were analysed. The obtained data indicates that the proposed device is able to detect changes in DR of the RBCs, allowing for distinguishing the samples from the healthy controls and the patients. Overall, the deformability of ESKD patients with and without diabetes type II is lower in comparison with the RBCs from the healthy controls, with this di erence being more evident for the group of ESKD patients with diabetes. RBCs from ESKD patients without diabetes elongate on average 8% less, within the hyperbolic contraction, as compared to healthy controls; whereas, RBCs from ESKD patients with diabetes elongate on average 14% less than the healthy controls. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to assess blood cells deformability due to the huge progress in image processing and high-speed microvisualization technology.application/pdfen_ENA microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannelFaustino, VeraRodrigues, Raquel OliveiraPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496Costa, ElísioSantos-Silva, AliceMiranda, VascoPersonalAmaral, Joana S.DSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdDSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdAmaralJoana S.Ciência IDhttps://www.ciencia-id.pt5319-7DE8-BEDAORCIDhttp://orcid.org0000-0002-3648-7303Lima, Rui A.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.3390/mi101006452018-01-19T10:00:00Z20192019-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/20250http://purl.org/coar/access_right/c_abf2open accessBlood on chipsCell deformabilityChronic renal diseaseDiabetesHyperbolic microchannelMicrofluidic devicesRed blood cells (RBCs)2233727 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/858c87e2-2719-4adc-be46-1a7b1e6734e7/download |
| spellingShingle | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel Faustino, Vera Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| subject.fl_str_mv | Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| title | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| title_full | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| title_fullStr | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| title_full_unstemmed | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| title_short | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| title_sort | A microfluidic deformability assessment of pathological red blood cells flowing in a hyperbolic converging microchannel |
| topic | Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| topic_facet | Blood on chips Cell deformability Chronic renal disease Diabetes Hyperbolic microchannel Microfluidic devices Red blood cells (RBCs) |
| url | http://hdl.handle.net/10198/20250 |
| visible | 1 |