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Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol
| Resumo: | Mushrooms are well known for their richness in bioactive molecules such as antioxidants. Phenolic compounds, in particular phenolic acids, have been the most widely studied molecules regarding these effects [1]. Nevertheless, other molecules present in mushrooms, such as ergosterol, can also display bioactive properties [2]. Although being this high-value molecule more associated with hypocholesterolemic, antimicrobial and anti-inflammatory effects [2], it is also relevant to screen other bioactivities, such as antioxidant activity, either of the pure molecule or of mycosterols' rich extracts containing it. Agaricus bisporus L. is the most consumed mushroom worldwide, being ergosterol the most abundant mycosterol in its sterol fraction (represents almost 90%) [3]. Herein, A. bisporus ethanol and hexane extracts were prepared by Soxhlet and ultrasound-assisted extraction (amplitude: 75%;sonication time: 15 min), and further evaluated for their antioxidant activity. The in vitro assays used were: i) 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH) assay, to evaluate the scavenging activity; and ii) ferricyanide Prussian blue assay, to evaluate the reducing power. The obtained extracts were characterized in terms of ergosterol content by HPLC-UV. The antioxidant activity of pure ergosterol was also assessed. The extracts obtained by Soxhlet showed higher antioxidant activity than the ones obtained by ultrassonication, which is in agreement with the higher leveis of ergosterol found in the flrst extracts (677 and 186 mg/100 g dw for ethanol and hexane extracts, respectively). Ethanol extracts revealed higher antioxidant activity than the hexane exfracts, which is also in agreement with the higher ergosterol content found in both samples (677 and 672 mg/100 g dw for Soxhlet and ultrassonication extracts, respectively). The pure ergosterol also showed antioxidant activity (e. g., DPPH ECso value = 0.46 mg/mL). Overall, the ethanol extract obtained by Soxhlet gave the highest DPPH scavenging activity (EC5o = 2.2 mg/mL) and reducing power (ECsa = 0.8 mg/mL), while the hexane extract obtained by ultrassonication revealed the lowest DPPH scavenging activity (ECso = 16.7 mg/mL) and reducing power (EC5o= 2.2 mg/mL). |
|---|---|
| Autores principais: | Moreno, Abília |
| Outros Autores: | Heleno, Sandrina A.; Barros, Lillian; Barreiro, M.F.; Ferreira, Isabel C.F.R. |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850829441662976 |
|---|---|
| author | Moreno, Abília |
| author2 | Heleno, Sandrina A. Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. |
| author2_role | author author author author |
| author_facet | Moreno, Abília Heleno, Sandrina A. Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Moreno, Abília\"},{\"Person.name\":\"Heleno, Sandrina A.\",\"Person.identifier.orcid\":\"0000-0001-7224-1098\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Barreiro, M.F.\",\"Person.identifier.orcid\":\"0000-0002-6844-333X\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Moreno, Abília Heleno, Sandrina A. Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-11-24T11:13:56Z |
| datacite.date.embargoed.fl_str_mv | 2015-11-24T11:13:56Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Moreno, Abília Heleno, Sandrina A. Barros, Lillian Barreiro, M.F. Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-11-24T11:13:56Z |
| dc.date.embargoed.fl_str_mv | 2015-11-24T11:13:56Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/12380 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Mushrooms are well known for their richness in bioactive molecules such as antioxidants. Phenolic compounds, in particular phenolic acids, have been the most widely studied molecules regarding these effects [1]. Nevertheless, other molecules present in mushrooms, such as ergosterol, can also display bioactive properties [2]. Although being this high-value molecule more associated with hypocholesterolemic, antimicrobial and anti-inflammatory effects [2], it is also relevant to screen other bioactivities, such as antioxidant activity, either of the pure molecule or of mycosterols' rich extracts containing it. Agaricus bisporus L. is the most consumed mushroom worldwide, being ergosterol the most abundant mycosterol in its sterol fraction (represents almost 90%) [3]. Herein, A. bisporus ethanol and hexane extracts were prepared by Soxhlet and ultrasound-assisted extraction (amplitude: 75%;sonication time: 15 min), and further evaluated for their antioxidant activity. The in vitro assays used were: i) 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH) assay, to evaluate the scavenging activity; and ii) ferricyanide Prussian blue assay, to evaluate the reducing power. The obtained extracts were characterized in terms of ergosterol content by HPLC-UV. The antioxidant activity of pure ergosterol was also assessed. The extracts obtained by Soxhlet showed higher antioxidant activity than the ones obtained by ultrassonication, which is in agreement with the higher leveis of ergosterol found in the flrst extracts (677 and 186 mg/100 g dw for ethanol and hexane extracts, respectively). Ethanol extracts revealed higher antioxidant activity than the hexane exfracts, which is also in agreement with the higher ergosterol content found in both samples (677 and 672 mg/100 g dw for Soxhlet and ultrassonication extracts, respectively). The pure ergosterol also showed antioxidant activity (e. g., DPPH ECso value = 0.46 mg/mL). Overall, the ethanol extract obtained by Soxhlet gave the highest DPPH scavenging activity (EC5o = 2.2 mg/mL) and reducing power (ECsa = 0.8 mg/mL), while the hexane extract obtained by ultrassonication revealed the lowest DPPH scavenging activity (ECso = 16.7 mg/mL) and reducing power (EC5o= 2.2 mg/mL). |
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| eu_rights_str_mv | openAccess |
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| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/f4240061-464e-402f-a735-96afccc7fa2c/download |
| id | ipb_bb508ebf4b3ddf50b3746bcc4060dc9f |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/12380 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/12380 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Moreno, Abília Heleno, Sandrina A. Heleno, Sandrina A. https://www.ciencia-id.pt/8B18-3095-6FC9 8B18-3095-6FC9 http://orcid.org/0000-0001-7224-1098 0000-0001-7224-1098 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Barreiro, M.F. Barreiro, M.F. https://www.ciencia-id.pt/3418-47D5-5746 3418-47D5-5746 http://orcid.org/0000-0002-6844-333X 0000-0002-6844-333X Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2015 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engpt_PTMushrooms are well known for their richness in bioactive molecules such as antioxidants. Phenolic compounds, in particular phenolic acids, have been the most widely studied molecules regarding these effects [1]. Nevertheless, other molecules present in mushrooms, such as ergosterol, can also display bioactive properties [2]. Although being this high-value molecule more associated with hypocholesterolemic, antimicrobial and anti-inflammatory effects [2], it is also relevant to screen other bioactivities, such as antioxidant activity, either of the pure molecule or of mycosterols' rich extracts containing it. Agaricus bisporus L. is the most consumed mushroom worldwide, being ergosterol the most abundant mycosterol in its sterol fraction (represents almost 90%) [3]. Herein, A. bisporus ethanol and hexane extracts were prepared by Soxhlet and ultrasound-assisted extraction (amplitude: 75%;sonication time: 15 min), and further evaluated for their antioxidant activity. The in vitro assays used were: i) 2, 2- diphenyl-1-picrylhydrazyl radical (DPPH) assay, to evaluate the scavenging activity; and ii) ferricyanide Prussian blue assay, to evaluate the reducing power. The obtained extracts were characterized in terms of ergosterol content by HPLC-UV. The antioxidant activity of pure ergosterol was also assessed. The extracts obtained by Soxhlet showed higher antioxidant activity than the ones obtained by ultrassonication, which is in agreement with the higher leveis of ergosterol found in the flrst extracts (677 and 186 mg/100 g dw for ethanol and hexane extracts, respectively). Ethanol extracts revealed higher antioxidant activity than the hexane exfracts, which is also in agreement with the higher ergosterol content found in both samples (677 and 672 mg/100 g dw for Soxhlet and ultrassonication extracts, respectively). The pure ergosterol also showed antioxidant activity (e. g., DPPH ECso value = 0.46 mg/mL). Overall, the ethanol extract obtained by Soxhlet gave the highest DPPH scavenging activity (EC5o = 2.2 mg/mL) and reducing power (ECsa = 0.8 mg/mL), while the hexane extract obtained by ultrassonication revealed the lowest DPPH scavenging activity (ECso = 16.7 mg/mL) and reducing power (EC5o= 2.2 mg/mL).application/pdfpt_PTAntioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterolMoreno, AbíliaPersonalHeleno, Sandrina A.DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676HelenoSandrina A.Ciência IDhttps://www.ciencia-id.pt8B18-3095-6FC9ORCIDhttp://orcid.org0000-0001-7224-1098Researcher IDhttps://www.researcherid.comL-5951-2014Scopus Author IDhttps://www.scopus.com30067731800PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalBarreiro, M.F.DSpacehttp://dspace.org/items/0391987c-ba90-488b-839a-153447ce1f0fDSpacehttp://dspace.org/items/0391987c-ba90-488b-839a-153447ce1f0fBarreiroFilomenaCiência IDhttps://www.ciencia-id.pt3418-47D5-5746ORCIDhttp://orcid.org0000-0002-6844-333XResearcher IDhttps://www.researcherid.comL-9802-2014Scopus Author IDhttps://www.scopus.com57188719717PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2015-11-24T11:13:56Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/12380http://purl.org/coar/access_right/c_abf2open access114307 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2015http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/f4240061-464e-402f-a735-96afccc7fa2c/download2nd Symposium on Medicinal Chemistry of University of Minho |
| spellingShingle | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol Moreno, Abília |
| title | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| title_full | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| title_fullStr | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| title_full_unstemmed | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| title_short | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| title_sort | Antioxidant activity of Agaricus bisporus L. hexane and ethanol extracts obtained by Soxhlet and ultrasound-assisted extraction: the importance of the presence of ergosterol |
| url | http://hdl.handle.net/10198/12380 |
| visible | 1 |