Publicação
Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations
| Resumo: | Varroa destructor is one of the main parasites of the honey bee (Apis mellifera), causing significant colony losses worldwide. The control of this mite often relies on acaricides, with amitraz being among the most widely used products in Portugal and Spain over the last decade. However, the continuous and persistent use of the same active substance exerts strong selective pressure on parasite populations, potentially favouring alleles associated with treatment resistance. Recently, a point mutation resulting in a phenylalanine-to-leucine substitution at position 290 (F290L) was identified in V. destructor populations from Spain, putatively associated with amitraz resistance. This mutation has been detected at high frequency in recent samples, suggesting resistance may have evolved due to sustained amitraz use. To assess whether the F290L mutation was already present before the widespread amitraz adoption, DNA extraction and sequencing analysis will be performed on mites collected since 2006, prior to the massive use of this treatment in Iberian beekeeping. This approach will clarify whether the allele frequency of F290L increased because of the selective pressure exerted by amitraz or if it already existed in high frequencies in earlier populations. This study will contribute to a better understanding of the evolution of resistance and to the development of more effective monitoring and management strategies to combat V. destructor. |
|---|---|
| Autores principais: | Costa, Maíra |
| Outros Autores: | Pérez-Pérez, António; Santos, Ana Clara; Prado, Rafaela; Lopes, Doralinda; Lopes, Ana Rita; Yadró Garcia, Carlos A.; Martín-Hernández, Raquel; Higes, Mariano; Pinto, M. Alice; Henriques, Dora |
| Assunto: | Varroa destructor Amitraz Resistance Mutation |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173394421645312 |
|---|---|
| author | Costa, Maíra |
| author2 | Pérez-Pérez, António Santos, Ana Clara Prado, Rafaela Lopes, Doralinda Lopes, Ana Rita Yadró Garcia, Carlos A. Martín-Hernández, Raquel Higes, Mariano Pinto, M. Alice Henriques, Dora |
| author2_role | author author author author author author author author author author |
| author_facet | Costa, Maíra Pérez-Pérez, António Santos, Ana Clara Prado, Rafaela Lopes, Doralinda Lopes, Ana Rita Yadró Garcia, Carlos A. Martín-Hernández, Raquel Higes, Mariano Pinto, M. Alice Henriques, Dora |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Costa, Maíra\",\"Person.identifier.orcid\":\"0000-0001-6697-6581\"},{\"Person.name\":\"Pérez-Pérez, António\"},{\"Person.name\":\"Santos, Ana Clara\"},{\"Person.name\":\"Prado, Rafaela\"},{\"Person.name\":\"Lopes, Doralinda\"},{\"Person.name\":\"Lopes, Ana Rita\",\"Person.identifier.orcid\":\"0000-0002-1888-2262\"},{\"Person.name\":\"Yadró Garcia, Carlos A.\",\"Person.identifier.orcid\":\"0000-0002-6916-3647\"},{\"Person.name\":\"Martín-Hernández, Raquel\"},{\"Person.name\":\"Higes, Mariano\"},{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Henriques, Dora\",\"Person.identifier.orcid\":\"0000-0001-7530-682X\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, Maíra Pérez-Pérez, António Santos, Ana Clara Prado, Rafaela Lopes, Doralinda Lopes, Ana Rita Yadró Garcia, Carlos A. Martín-Hernández, Raquel Higes, Mariano Pinto, M. Alice Henriques, Dora |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-02-12T14:25:00Z |
| datacite.date.embargoed.fl_str_mv | 2026-02-12T14:25:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Varroa destructor Amitraz Resistance Mutation |
| datacite.titles.title.fl_str_mv | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Costa, Maíra Pérez-Pérez, António Santos, Ana Clara Prado, Rafaela Lopes, Doralinda Lopes, Ana Rita Yadró Garcia, Carlos A. Martín-Hernández, Raquel Higes, Mariano Pinto, M. Alice Henriques, Dora |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-02-12T14:25:00Z |
| dc.date.embargoed.fl_str_mv | 2026-02-12T14:25:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/35732 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Varroa destructor Amitraz Resistance Mutation |
| dc.title.fl_str_mv | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Varroa destructor is one of the main parasites of the honey bee (Apis mellifera), causing significant colony losses worldwide. The control of this mite often relies on acaricides, with amitraz being among the most widely used products in Portugal and Spain over the last decade. However, the continuous and persistent use of the same active substance exerts strong selective pressure on parasite populations, potentially favouring alleles associated with treatment resistance. Recently, a point mutation resulting in a phenylalanine-to-leucine substitution at position 290 (F290L) was identified in V. destructor populations from Spain, putatively associated with amitraz resistance. This mutation has been detected at high frequency in recent samples, suggesting resistance may have evolved due to sustained amitraz use. To assess whether the F290L mutation was already present before the widespread amitraz adoption, DNA extraction and sequencing analysis will be performed on mites collected since 2006, prior to the massive use of this treatment in Iberian beekeeping. This approach will clarify whether the allele frequency of F290L increased because of the selective pressure exerted by amitraz or if it already existed in high frequencies in earlier populations. This study will contribute to a better understanding of the evolution of resistance and to the development of more effective monitoring and management strategies to combat V. destructor. |
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| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/ac2a6dbc-687b-4935-b6fc-0c4d784aca5a/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ipb_bc1f77b0569ed6c753fe697dfde2cc9e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/35732 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/35732 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Costa, Maíra Costa, Maíra https://www.ciencia-id.pt/FB10-3DFA-D593 FB10-3DFA-D593 http://orcid.org/0000-0001-6697-6581 0000-0001-6697-6581 Pérez-Pérez, António Santos, Ana Clara Prado, Rafaela Lopes, Doralinda Lopes, Ana Rita Lopes, Ana Rita https://www.ciencia-id.pt/F014-6835-054D F014-6835-054D http://orcid.org/0000-0002-1888-2262 0000-0002-1888-2262 Yadró Garcia, Carlos A. Yadró Garcia, Carlos A. https://www.ciencia-id.pt/F71F-B08E-EC39 F71F-B08E-EC39 http://orcid.org/0000-0002-6916-3647 0000-0002-6916-3647 Martín-Hernández, Raquel Higes, Mariano Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Henriques, Dora Henriques, Dora https://www.ciencia-id.pt/291F-986F-07DA 291F-986F-07DA http://orcid.org/0000-0001-7530-682X 0000-0001-7530-682X |
| publishDate | 2025 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
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| spelling | engengVarroa destructor is one of the main parasites of the honey bee (Apis mellifera), causing significant colony losses worldwide. The control of this mite often relies on acaricides, with amitraz being among the most widely used products in Portugal and Spain over the last decade. However, the continuous and persistent use of the same active substance exerts strong selective pressure on parasite populations, potentially favouring alleles associated with treatment resistance. Recently, a point mutation resulting in a phenylalanine-to-leucine substitution at position 290 (F290L) was identified in V. destructor populations from Spain, putatively associated with amitraz resistance. This mutation has been detected at high frequency in recent samples, suggesting resistance may have evolved due to sustained amitraz use. To assess whether the F290L mutation was already present before the widespread amitraz adoption, DNA extraction and sequencing analysis will be performed on mites collected since 2006, prior to the massive use of this treatment in Iberian beekeeping. This approach will clarify whether the allele frequency of F290L increased because of the selective pressure exerted by amitraz or if it already existed in high frequencies in earlier populations. This study will contribute to a better understanding of the evolution of resistance and to the development of more effective monitoring and management strategies to combat V. destructor.application/pdfengEvolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populationsPersonalCosta, MaíraDSpacehttp://dspace.org/items/c7b97b29-3139-43ff-ae85-23f613e280edDSpacehttp://dspace.org/items/c7b97b29-3139-43ff-ae85-23f613e280edCostaMaíraCiência IDhttps://www.ciencia-id.ptFB10-3DFA-D593ORCIDhttp://orcid.org0000-0001-6697-6581Pérez-Pérez, AntónioSantos, Ana ClaraPrado, RafaelaLopes, DoralindaPersonalLopes, Ana RitaDSpacehttp://dspace.org/items/5c8dc714-e63c-4ac6-882e-92deafa4bbe8DSpacehttp://dspace.org/items/5c8dc714-e63c-4ac6-882e-92deafa4bbe8LopesAna RitaCiência IDhttps://www.ciencia-id.ptF014-6835-054DORCIDhttp://orcid.org0000-0002-1888-2262PersonalYadró Garcia, Carlos A.DSpacehttp://dspace.org/items/59212a6c-fc6a-45fd-b37a-51e90926c9e3DSpacehttp://dspace.org/items/59212a6c-fc6a-45fd-b37a-51e90926c9e3Yadró GarciaCarlos A.Ciência IDhttps://www.ciencia-id.ptF71F-B08E-EC39ORCIDhttp://orcid.org0000-0002-6916-3647Martín-Hernández, RaquelHiges, MarianoPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800PersonalHenriques, DoraDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dHenriquesDoraCiência IDhttps://www.ciencia-id.pt291F-986F-07DAORCIDhttp://orcid.org0000-0001-7530-682XScopus Author IDhttps://www.scopus.com55761737300HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-972-745-368-92026-02-12T14:25:00Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/35732http://purl.org/coar/access_right/c_abf2open accessVarroa destructorAmitrazResistanceMutation720272 bytesFundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regions6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/ac2a6dbc-687b-4935-b6fc-0c4d784aca5a/downloadXXI International Meeting of the Portuguese Association for Evolutionary BiologyBragança, Portugal2025 |
| spellingShingle | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations Costa, Maíra Varroa destructor Amitraz Resistance Mutation |
| status | SINGLETON |
| subject.fl_str_mv | Varroa destructor Amitraz Resistance Mutation |
| title | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| title_full | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| title_fullStr | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| title_full_unstemmed | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| title_short | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| title_sort | Evolution of amitraz resistance in varroa destructor: historical assessment of the F290L mutation in iberian populations |
| topic | Varroa destructor Amitraz Resistance Mutation |
| topic_facet | Varroa destructor Amitraz Resistance Mutation |
| url | http://hdl.handle.net/10198/35732 |
| visible | 1 |