Publicação
The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties
| Resumo: | Being Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analysed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and anti-proliferative properties in different human tumor cell lines. Mannitol (2.01 g/100 g dw) and trehalose (24.71 g/100 g) were the free sugars found in C. militaris. Polyunsaturated fatty acids (68.87%) predominated over saturated fatty acids (23.40%) and δ-tocopherol was the only isoform of vitamin E detected (55.86 μg/100 g). The organic acids found in this mushroom were oxalic, citric and fumaric acids (0.33, 7.97 and 0.13 g/100g, respectively). p-Hydroxybenzoic acid was the only phenolic acid quantified in this species (0.02 mg/100 g); although cinnamic acid was also found (0.11 mg/100 g). The methanolic extract of C. militaris proved to inhibit lipid peroxidation, have reducing power and scavenge free radicals. This extract also revealed strong antibacterial and antifungal properties. Finally, the C. militaris extract was able to inhibit the proliferation of MCF-7 (breast), NCI-H460 (non-small lung), HCT-15 (colon) and HeLa (cervical) human carcinoma cell lines. |
|---|---|
| Autores principais: | Reis, Filipa S. |
| Outros Autores: | Barros, Lillian; Calhelha, Ricardo C.; Ćirić, Ana; Van Griensven, Leo J.L.D.; Soković, Marina; Ferreira, Isabel C.F.R. |
| Assunto: | Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| Ano: | 2013 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851219596869632 |
|---|---|
| author | Reis, Filipa S. |
| author2 | Barros, Lillian Calhelha, Ricardo C. Ćirić, Ana Van Griensven, Leo J.L.D. Soković, Marina Ferreira, Isabel C.F.R. |
| author2_role | author author author author author author |
| author_facet | Reis, Filipa S. Barros, Lillian Calhelha, Ricardo C. Ćirić, Ana Van Griensven, Leo J.L.D. Soković, Marina Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Reis, Filipa S.\",\"Person.identifier.orcid\":\"0000-0002-9159-0530\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Ćirić, Ana\"},{\"Person.name\":\"Van Griensven, Leo J.L.D.\"},{\"Person.name\":\"Soković, Marina\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Reis, Filipa S. Barros, Lillian Calhelha, Ricardo C. Ćirić, Ana Van Griensven, Leo J.L.D. Soković, Marina Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2014-05-13T16:52:51Z |
| datacite.date.embargoed.fl_str_mv | 2014-05-13T16:52:51Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| datacite.titles.title.fl_str_mv | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Reis, Filipa S. Barros, Lillian Calhelha, Ricardo C. Ćirić, Ana Van Griensven, Leo J.L.D. Soković, Marina Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2013-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2014-05-13T16:52:51Z |
| dc.date.embargoed.fl_str_mv | 2014-05-13T16:52:51Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/9507 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Elsevier |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| dc.title.fl_str_mv | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Being Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analysed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and anti-proliferative properties in different human tumor cell lines. Mannitol (2.01 g/100 g dw) and trehalose (24.71 g/100 g) were the free sugars found in C. militaris. Polyunsaturated fatty acids (68.87%) predominated over saturated fatty acids (23.40%) and δ-tocopherol was the only isoform of vitamin E detected (55.86 μg/100 g). The organic acids found in this mushroom were oxalic, citric and fumaric acids (0.33, 7.97 and 0.13 g/100g, respectively). p-Hydroxybenzoic acid was the only phenolic acid quantified in this species (0.02 mg/100 g); although cinnamic acid was also found (0.11 mg/100 g). The methanolic extract of C. militaris proved to inhibit lipid peroxidation, have reducing power and scavenge free radicals. This extract also revealed strong antibacterial and antifungal properties. Finally, the C. militaris extract was able to inhibit the proliferation of MCF-7 (breast), NCI-H460 (non-small lung), HCT-15 (colon) and HeLa (cervical) human carcinoma cell lines. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/7d4af438-99c6-4f65-a00d-737e831c48d3/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 5876-PPCDTI 6817 - DCRRNI ID |
| id | ipb_bc646c3f0dc98239283ea5ffacf1cbf6 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/9507 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/9507 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Reis, Filipa S. Reis, Filipa S. https://www.ciencia-id.pt/391F-AFE1-64C7 391F-AFE1-64C7 http://orcid.org/0000-0002-9159-0530 0000-0002-9159-0530 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Ćirić, Ana Van Griensven, Leo J.L.D. Soković, Marina Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2013 |
| publisher.none.fl_str_mv | Elsevier |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engElsevierporBeing Cordyceps militaris (L.) Link recognized as a medicinal and edible mushroom, this work intends to reveal new interesting bioactive molecules that could be isolated from this species. Hydrophilic and lipophilic compounds were analysed by chromatographic techniques coupled to different detectors. The methanolic extract of C. militaris was tested for its antioxidant, antibacterial, antifungal and anti-proliferative properties in different human tumor cell lines. Mannitol (2.01 g/100 g dw) and trehalose (24.71 g/100 g) were the free sugars found in C. militaris. Polyunsaturated fatty acids (68.87%) predominated over saturated fatty acids (23.40%) and δ-tocopherol was the only isoform of vitamin E detected (55.86 μg/100 g). The organic acids found in this mushroom were oxalic, citric and fumaric acids (0.33, 7.97 and 0.13 g/100g, respectively). p-Hydroxybenzoic acid was the only phenolic acid quantified in this species (0.02 mg/100 g); although cinnamic acid was also found (0.11 mg/100 g). The methanolic extract of C. militaris proved to inhibit lipid peroxidation, have reducing power and scavenge free radicals. This extract also revealed strong antibacterial and antifungal properties. Finally, the C. militaris extract was able to inhibit the proliferation of MCF-7 (breast), NCI-H460 (non-small lung), HCT-15 (colon) and HeLa (cervical) human carcinoma cell lines.application/pdfapplication/pdfporThe methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines propertiesPersonalReis, Filipa S.DSpacehttp://dspace.org/items/b8d384ae-2134-4735-93a6-0d2febbf9220DSpacehttp://dspace.org/items/b8d384ae-2134-4735-93a6-0d2febbf9220ReisFilipa S.Ciência IDhttps://www.ciencia-id.pt391F-AFE1-64C7ORCIDhttp://orcid.org0000-0002-9159-0530Researcher IDhttps://www.researcherid.comI-2093-2013Scopus Author IDhttps://www.scopus.com36982144400PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333Ćirić, AnaVan Griensven, Leo J.L.D.Soković, MarinaPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0278-6915DOIIsPartOfhttp://dx.doi.org/10.1016/j.fct.2013.08.0332014-05-13T16:52:51Z20132013-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/9507http://purl.org/coar/access_right/c_abf2open accessMedicinal/edible mushroomCordyceps militarisChemical characterizationAntioxidant potentialAntimicrobial activityAnti-proliferative properties393827 bytes401299 bytesFundação para a Ciência e a TecnologiaPortuguese Wild Mushrooms: Chemical characterization and functional study of antiproliferative and proapoptotic properties in cancer cell lines5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaStrategic Project - UI 690 - 2011-20126817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/7d4af438-99c6-4f65-a00d-737e831c48d3/downloadhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/ed5871d7-f871-495b-aa0e-21a2fbe0c4a1/downloadFood and Chemical Toxicology629198 |
| spellingShingle | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties Reis, Filipa S. Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| subject.fl_str_mv | Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| title | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| title_full | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| title_fullStr | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| title_full_unstemmed | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| title_short | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| title_sort | The methanolic extract of Cordyceps militaris (L.) Link fruiting body shows antioxidant, antibacterial, antifungal and anti human tumor cell lines properties |
| topic | Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| topic_facet | Medicinal/edible mushroom Cordyceps militaris Chemical characterization Antioxidant potential Antimicrobial activity Anti-proliferative properties |
| url | http://hdl.handle.net/10198/9507 |
| visible | 1 |