Publicação
Image processing of petri dishes for counting microorganisms
| Resumo: | For a food to be considered functional it is necessary to prove that it has microorganisms in its composition. In order to determine the presence of microorganisms in a food, laboratory analyzes can be carried out using of Petri dishes, which must pass through an incubation period, to then manually count the number of bacterial colonies that are present in each board. Therefore, the objective of this work was to develop a software to perform the automated counting of colonies contained in Petri plates and to validate the efficiency of the tool through comparisons with the results of a manual count. For this, an algorithm was developed based on digital image processing techniques capable of identifying the Petri dish within the image and counting the number of colonies present on each plate. As a result, a global correlation of 0.948 was observed in relation to the manual count, and in an individual analysis, a correlation of 0.8134 was obtained. Thus, it can be concluded that counts of microorganisms can be performed automatically and reliably with the developed software. |
|---|---|
| Autores principais: | Barbosa, Marcela |
| Outros Autores: | Santos, Everton; Teixeira, João Paulo; Mendonca, Saraspathy; Candido Junior, Arnaldo; Paula Filho, Pedro Luiz de |
| Assunto: | Petri counting Digital image processing |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172879076950016 |
|---|---|
| author | Barbosa, Marcela |
| author2 | Santos, Everton Teixeira, João Paulo Mendonca, Saraspathy Candido Junior, Arnaldo Paula Filho, Pedro Luiz de |
| author2_role | author author author author author |
| author_facet | Barbosa, Marcela Santos, Everton Teixeira, João Paulo Mendonca, Saraspathy Candido Junior, Arnaldo Paula Filho, Pedro Luiz de |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Barbosa, Marcela\"},{\"Person.name\":\"Santos, Everton\"},{\"Person.name\":\"Teixeira, João Paulo\",\"Person.identifier.orcid\":\"0000-0002-6679-5702\"},{\"Person.name\":\"Mendonca, Saraspathy\"},{\"Person.name\":\"Candido Junior, Arnaldo\"},{\"Person.name\":\"Paula Filho, Pedro Luiz de\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Barbosa, Marcela Santos, Everton Teixeira, João Paulo Mendonca, Saraspathy Candido Junior, Arnaldo Paula Filho, Pedro Luiz de |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-16T09:32:05Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-16T09:32:05Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | Petri counting Digital image processing |
| datacite.titles.title.fl_str_mv | Image processing of petri dishes for counting microorganisms |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Barbosa, Marcela Santos, Everton Teixeira, João Paulo Mendonca, Saraspathy Candido Junior, Arnaldo Paula Filho, Pedro Luiz de |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-16T09:32:05Z |
| dc.date.embargoed.fl_str_mv | 2023-03-16T09:32:05Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/27763 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Springer Nature |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | Petri counting Digital image processing |
| dc.title.fl_str_mv | Image processing of petri dishes for counting microorganisms |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | For a food to be considered functional it is necessary to prove that it has microorganisms in its composition. In order to determine the presence of microorganisms in a food, laboratory analyzes can be carried out using of Petri dishes, which must pass through an incubation period, to then manually count the number of bacterial colonies that are present in each board. Therefore, the objective of this work was to develop a software to perform the automated counting of colonies contained in Petri plates and to validate the efficiency of the tool through comparisons with the results of a manual count. For this, an algorithm was developed based on digital image processing techniques capable of identifying the Petri dish within the image and counting the number of colonies present on each plate. As a result, a global correlation of 0.948 was observed in relation to the manual count, and in an individual analysis, a correlation of 0.8134 was obtained. Thus, it can be concluded that counts of microorganisms can be performed automatically and reliably with the developed software. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | conferencePaper |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/3a1a9395-59e6-4f5c-a777-695b08b9607c/download |
| id | ipb_bd2a006e45ef772e5886f6e2f673aeab |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/27763 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/27763 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Barbosa, Marcela Santos, Everton Teixeira, João Paulo Teixeira, João Paulo https://www.ciencia-id.pt/4F15-B322-59B4 4F15-B322-59B4 http://orcid.org/0000-0002-6679-5702 0000-0002-6679-5702 Mendonca, Saraspathy Candido Junior, Arnaldo Paula Filho, Pedro Luiz de |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Springer Nature |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engSpringer Naturept_PTFor a food to be considered functional it is necessary to prove that it has microorganisms in its composition. In order to determine the presence of microorganisms in a food, laboratory analyzes can be carried out using of Petri dishes, which must pass through an incubation period, to then manually count the number of bacterial colonies that are present in each board. Therefore, the objective of this work was to develop a software to perform the automated counting of colonies contained in Petri plates and to validate the efficiency of the tool through comparisons with the results of a manual count. For this, an algorithm was developed based on digital image processing techniques capable of identifying the Petri dish within the image and counting the number of colonies present on each plate. As a result, a global correlation of 0.948 was observed in relation to the manual count, and in an individual analysis, a correlation of 0.8134 was obtained. Thus, it can be concluded that counts of microorganisms can be performed automatically and reliably with the developed software.application/pdfpt_PTImage processing of petri dishes for counting microorganismsBarbosa, MarcelaSantos, EvertonPersonalTeixeira, João PauloDSpacehttp://dspace.org/items/33f4af65-7ddf-46f0-8b44-a7470a8ba2bfDSpacehttp://dspace.org/items/33f4af65-7ddf-46f0-8b44-a7470a8ba2bfTeixeiraJoão PauloCiência IDhttps://www.ciencia-id.pt4F15-B322-59B4ORCIDhttp://orcid.org0000-0002-6679-5702Researcher IDhttps://www.researcherid.comN-6576-2013Scopus Author IDhttps://www.scopus.com57069567500Mendonca, SaraspathyCandido Junior, ArnaldoPaula Filho, Pedro Luiz deHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.1007/978-3-031-23236-7_522023-03-16T09:32:05Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27763http://purl.org/coar/access_right/c_16ecrestricted accessPetri countingDigital image processing3994730 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paper2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/3a1a9395-59e6-4f5c-a777-695b08b9607c/downloadSecond International Conference, OL2A 20221754763775Bragança |
| spellingShingle | Image processing of petri dishes for counting microorganisms Barbosa, Marcela Petri counting Digital image processing |
| status | SINGLETON |
| subject.fl_str_mv | Petri counting Digital image processing |
| title | Image processing of petri dishes for counting microorganisms |
| title_full | Image processing of petri dishes for counting microorganisms |
| title_fullStr | Image processing of petri dishes for counting microorganisms |
| title_full_unstemmed | Image processing of petri dishes for counting microorganisms |
| title_short | Image processing of petri dishes for counting microorganisms |
| title_sort | Image processing of petri dishes for counting microorganisms |
| topic | Petri counting Digital image processing |
| topic_facet | Petri counting Digital image processing |
| url | http://hdl.handle.net/10198/27763 |
| visible | 1 |