Publicação
Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs
| Resumo: | No other honey bee subspecies has caught so much attention from evolutionary biologists as Apis mellifera iberiensis. For over 25 years, Iberian honey bee populations have been surveyed using a wide range of markers, including morphometry, allozymes, mtDNA, microsatellites, and more recently SNPs. Despite the numerous studies, diversity patterns of Iberian honey bees are still not fully dissected and understood. In this study we performed a fine-scale sampling at both geographical and genomic level. Samples from over 711 colonies were collected across three north-south transects. These samples were sequenced for the mitochondrial tRNA"“-cox2 intergenic region, scored using geometric morphometrics of forewings, and genotyped for over 383 SNP loci. The three sets of markers concurrently supported the existence of a north-south cline, a pattern that contrasts with the absence of structure inferred from microsatellites. To test the hypothesis that selection has shaped and maintained the nuclear cline, we employed four PST-based outlier methods on the SNP data set. These analyses enabled detection of 74 outlier loci, which supports the selection hypothesis. However, neutral structure, inferred following removal of outliers, was able to recapture the north-south cline, which suggests that selection is not the only evolutionary force shaping the Iberian cline. Our findings further highlight the complexity of the Iberian honey bee diversity patterns and reinforce the importance of this southernmost European territory as a reservoir of Apis mellifera genetic diversity, a resource increasingly important in a rapidly changing and demanding world. |
|---|---|
| Autores principais: | Pinto, M. Alice |
| Outros Autores: | Chávez-Galarza, Julio; Henriques, Dora; Francoy, Tiago M.; Rufino, José; Muñoz, Irene; De la Rúa, Pilar; Johnston, J. Spencer |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173074910052352 |
|---|---|
| author | Pinto, M. Alice |
| author2 | Chávez-Galarza, Julio Henriques, Dora Francoy, Tiago M. Rufino, José Muñoz, Irene De la Rúa, Pilar Johnston, J. Spencer |
| author2_role | author author author author author author author |
| author_facet | Pinto, M. Alice Chávez-Galarza, Julio Henriques, Dora Francoy, Tiago M. Rufino, José Muñoz, Irene De la Rúa, Pilar Johnston, J. Spencer |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Chávez-Galarza, Julio\"},{\"Person.name\":\"Henriques, Dora\",\"Person.identifier.orcid\":\"0000-0001-7530-682X\"},{\"Person.name\":\"Francoy, Tiago M.\"},{\"Person.name\":\"Rufino, José\",\"Person.identifier.orcid\":\"0000-0002-1344-8264\"},{\"Person.name\":\"Muñoz, Irene\"},{\"Person.name\":\"De la Rúa, Pilar\"},{\"Person.name\":\"Johnston, J. Spencer\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pinto, M. Alice Chávez-Galarza, Julio Henriques, Dora Francoy, Tiago M. Rufino, José Muñoz, Irene De la Rúa, Pilar Johnston, J. Spencer |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-03-28T09:37:20Z |
| datacite.date.embargoed.fl_str_mv | 2018-03-28T09:37:20Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pinto, M. Alice Chávez-Galarza, Julio Henriques, Dora Francoy, Tiago M. Rufino, José Muñoz, Irene De la Rúa, Pilar Johnston, J. Spencer |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-03-28T09:37:20Z |
| dc.date.embargoed.fl_str_mv | 2018-03-28T09:37:20Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/16607 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Universidad de Murcia |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | No other honey bee subspecies has caught so much attention from evolutionary biologists as Apis mellifera iberiensis. For over 25 years, Iberian honey bee populations have been surveyed using a wide range of markers, including morphometry, allozymes, mtDNA, microsatellites, and more recently SNPs. Despite the numerous studies, diversity patterns of Iberian honey bees are still not fully dissected and understood. In this study we performed a fine-scale sampling at both geographical and genomic level. Samples from over 711 colonies were collected across three north-south transects. These samples were sequenced for the mitochondrial tRNA"“-cox2 intergenic region, scored using geometric morphometrics of forewings, and genotyped for over 383 SNP loci. The three sets of markers concurrently supported the existence of a north-south cline, a pattern that contrasts with the absence of structure inferred from microsatellites. To test the hypothesis that selection has shaped and maintained the nuclear cline, we employed four PST-based outlier methods on the SNP data set. These analyses enabled detection of 74 outlier loci, which supports the selection hypothesis. However, neutral structure, inferred following removal of outliers, was able to recapture the north-south cline, which suggests that selection is not the only evolutionary force shaping the Iberian cline. Our findings further highlight the complexity of the Iberian honey bee diversity patterns and reinforce the importance of this southernmost European territory as a reservoir of Apis mellifera genetic diversity, a resource increasingly important in a rapidly changing and demanding world. |
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| person_str_mv | Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Chávez-Galarza, Julio Henriques, Dora Henriques, Dora https://www.ciencia-id.pt/291F-986F-07DA 291F-986F-07DA http://orcid.org/0000-0001-7530-682X 0000-0001-7530-682X Francoy, Tiago M. Rufino, José Rufino, José https://www.ciencia-id.pt/C414-F47F-6323 C414-F47F-6323 http://orcid.org/0000-0002-1344-8264 0000-0002-1344-8264 Muñoz, Irene De la Rúa, Pilar Johnston, J. Spencer |
| publishDate | 2014 |
| publisher.none.fl_str_mv | Universidad de Murcia |
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| spelling | engUniversidad de Murciapt_PTNo other honey bee subspecies has caught so much attention from evolutionary biologists as Apis mellifera iberiensis. For over 25 years, Iberian honey bee populations have been surveyed using a wide range of markers, including morphometry, allozymes, mtDNA, microsatellites, and more recently SNPs. Despite the numerous studies, diversity patterns of Iberian honey bees are still not fully dissected and understood. In this study we performed a fine-scale sampling at both geographical and genomic level. Samples from over 711 colonies were collected across three north-south transects. These samples were sequenced for the mitochondrial tRNA"“-cox2 intergenic region, scored using geometric morphometrics of forewings, and genotyped for over 383 SNP loci. The three sets of markers concurrently supported the existence of a north-south cline, a pattern that contrasts with the absence of structure inferred from microsatellites. To test the hypothesis that selection has shaped and maintained the nuclear cline, we employed four PST-based outlier methods on the SNP data set. These analyses enabled detection of 74 outlier loci, which supports the selection hypothesis. However, neutral structure, inferred following removal of outliers, was able to recapture the north-south cline, which suggests that selection is not the only evolutionary force shaping the Iberian cline. Our findings further highlight the complexity of the Iberian honey bee diversity patterns and reinforce the importance of this southernmost European territory as a reservoir of Apis mellifera genetic diversity, a resource increasingly important in a rapidly changing and demanding world.application/pdfpt_PTPopulation structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPsPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800Chávez-Galarza, JulioPersonalHenriques, DoraDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dDSpacehttp://dspace.org/items/d2abd09f-a90c-4cfb-9a60-7fc32f56184dHenriquesDoraCiência IDhttps://www.ciencia-id.pt291F-986F-07DAORCIDhttp://orcid.org0000-0001-7530-682XScopus Author IDhttps://www.scopus.com55761737300Francoy, Tiago M.PersonalRufino, JoséDSpacehttp://dspace.org/items/1e24d2ce-a354-442a-bef8-eebadd94b385DSpacehttp://dspace.org/items/1e24d2ce-a354-442a-bef8-eebadd94b385RufinoJoséCiência IDhttps://www.ciencia-id.ptC414-F47F-6323ORCIDhttp://orcid.org0000-0002-1344-8264Scopus Author IDhttps://www.scopus.com55947199100Scopus Author IDhttps://www.scopus.com57188967176Muñoz, IreneDe la Rúa, PilarJohnston, J. SpencerHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-84-697-0855-22018-03-28T09:37:20Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/16607http://purl.org/coar/access_right/c_abf2open access1129548 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2014http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/531b3b92-fbfa-4997-b31a-f8e28c068874/downloadBook of Abstracts of the 6th European Conference of Apidology (EURBEE 2014)5454Murcia, Spain |
| spellingShingle | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs Pinto, M. Alice |
| status | SINGLETON |
| title | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| title_full | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| title_fullStr | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| title_full_unstemmed | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| title_short | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| title_sort | Population structure of Iberian honey bees: concordance among mtDNA, geometric morphometrics and SNPs |
| url | http://hdl.handle.net/10198/16607 |
| visible | 1 |