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Microbiota assessment of fresh-cut apples packaged in two different films

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Resumo:The aim of this work was to assess the natural microbiota of packed fresh-cut apples during refrigerated storage. Two different films were tested for the package, a biodegradable (PLA) film and a conventional and commercial one (OPP). Two antioxidant additives were applied, a natural olive pomace extract and the commercial ascorbic acid used by the industries. The results revealed lower bacteria counts in samples with olive pomace extract and PLA films than in those with ascorbic acid and OPP films after 5 and 12 days of storage. These findings suggest that the use of such natural extracts as additives in fruits could delay the growth of mesophilic bacteria. The characterization and identification of the bacterial isolates from fresh-cut apple samples showed that the most prevalent species were Citrobacter freundii, Staphylococcus warneri, Pseudomonas oryzihabitans, Alcalinogenes faecalis, Corynebacterium jeikeium, Micrococcus spp., Pantoea aglomerans and Bacillus spp. Furthermore, an increase in the microbial diversity during the storage time at refrigerated temperatures was observed, except for the sample treated with olive pomace extract and packaged in OPP film. The highest microbial diversity was found for samples with ascorbic acid as an additive. This could indicate a negative effect of ascorbic acid on the microbial inhibition of apple slices. The natural olive pomace extract demonstrated potential as an antimicrobial additive for fresh-cut apples.
Autores principais:Madureira, Joana
Outros Autores:Gonçalves, Sara; Santos-Buelga, Celestino; Margaça, Fernanda M.A.; Ferreira, Isabel C.F.R.; Barros, Lillian; Cabo Verde, Sandra
Assunto:Minimally processed apple Natural extracts Packaging Natural microbiota
Ano:2023
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Madureira, Joana
author2 Gonçalves, Sara
Santos-Buelga, Celestino
Margaça, Fernanda M.A.
Ferreira, Isabel C.F.R.
Barros, Lillian
Cabo Verde, Sandra
author2_role author
author
author
author
author
author
author_facet Madureira, Joana
Gonçalves, Sara
Santos-Buelga, Celestino
Margaça, Fernanda M.A.
Ferreira, Isabel C.F.R.
Barros, Lillian
Cabo Verde, Sandra
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Madureira, Joana\"},{\"Person.name\":\"Gonçalves, Sara\"},{\"Person.name\":\"Santos-Buelga, Celestino\"},{\"Person.name\":\"Margaça, Fernanda M.A.\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Cabo Verde, Sandra\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Madureira, Joana
Gonçalves, Sara
Santos-Buelga, Celestino
Margaça, Fernanda M.A.
Ferreira, Isabel C.F.R.
Barros, Lillian
Cabo Verde, Sandra
datacite.date.Accepted.fl_str_mv 2023-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2019-10-28T11:05:30Z
datacite.date.embargoed.fl_str_mv 2019-10-28T11:05:30Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Minimally processed apple
Natural extracts
Packaging
Natural microbiota
datacite.titles.title.fl_str_mv Microbiota assessment of fresh-cut apples packaged in two different films
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Madureira, Joana
Gonçalves, Sara
Santos-Buelga, Celestino
Margaça, Fernanda M.A.
Ferreira, Isabel C.F.R.
Barros, Lillian
Cabo Verde, Sandra
dc.date.Accepted.fl_str_mv 2023-01-01T00:00:00Z
dc.date.available.fl_str_mv 2019-10-28T11:05:30Z
dc.date.embargoed.fl_str_mv 2019-10-28T11:05:30Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/19701
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv MDPI
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Minimally processed apple
Natural extracts
Packaging
Natural microbiota
dc.title.fl_str_mv Microbiota assessment of fresh-cut apples packaged in two different films
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description The aim of this work was to assess the natural microbiota of packed fresh-cut apples during refrigerated storage. Two different films were tested for the package, a biodegradable (PLA) film and a conventional and commercial one (OPP). Two antioxidant additives were applied, a natural olive pomace extract and the commercial ascorbic acid used by the industries. The results revealed lower bacteria counts in samples with olive pomace extract and PLA films than in those with ascorbic acid and OPP films after 5 and 12 days of storage. These findings suggest that the use of such natural extracts as additives in fruits could delay the growth of mesophilic bacteria. The characterization and identification of the bacterial isolates from fresh-cut apple samples showed that the most prevalent species were Citrobacter freundii, Staphylococcus warneri, Pseudomonas oryzihabitans, Alcalinogenes faecalis, Corynebacterium jeikeium, Micrococcus spp., Pantoea aglomerans and Bacillus spp. Furthermore, an increase in the microbial diversity during the storage time at refrigerated temperatures was observed, except for the sample treated with olive pomace extract and packaged in OPP film. The highest microbial diversity was found for samples with ascorbic acid as an additive. This could indicate a negative effect of ascorbic acid on the microbial inhibition of apple slices. The natural olive pomace extract demonstrated potential as an antimicrobial additive for fresh-cut apples.
dirty 0
eu_rights_str_mv openAccess
format article
fulltext.url.fl_str_mv https://bibliotecadigital.ipb.pt/bitstreams/fd0f171e-fa48-4a31-95f9-ecb8d4137689/download
id ipb_c53e2b50fc0c07c04be759cda807d2d1
identifier.url.fl_str_mv http://hdl.handle.net/10198/19701
instacron_str ipb
institution Instituto Politécnico de Bragança
instname_str Instituto Politécnico de Bragança
language eng
network_acronym_str ipb
network_name_str Biblioteca Digital do IPB
oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/19701
organization_str_mv urn:organizationAcronym:ipb
person_str_mv Madureira, Joana
Gonçalves, Sara
Santos-Buelga, Celestino
Margaça, Fernanda M.A.
Ferreira, Isabel C.F.R.
Ferreira, Isabel C.F.R.
https://www.ciencia-id.pt/9418-CF95-9919
9418-CF95-9919
http://orcid.org/0000-0003-4910-4882
0000-0003-4910-4882
Barros, Lillian
Barros, Lillian
https://www.ciencia-id.pt/9616-35CB-D001
9616-35CB-D001
http://orcid.org/0000-0002-9050-5189
0000-0002-9050-5189
Cabo Verde, Sandra
publishDate 2023
publisher.none.fl_str_mv MDPI
reponame_str Biblioteca Digital do IPB
repository_id_str urn:repositoryAcronym:ipb
service_str_mv urn:repositoryAcronym:ipb
spelling engMDPIpt_PTThe aim of this work was to assess the natural microbiota of packed fresh-cut apples during refrigerated storage. Two different films were tested for the package, a biodegradable (PLA) film and a conventional and commercial one (OPP). Two antioxidant additives were applied, a natural olive pomace extract and the commercial ascorbic acid used by the industries. The results revealed lower bacteria counts in samples with olive pomace extract and PLA films than in those with ascorbic acid and OPP films after 5 and 12 days of storage. These findings suggest that the use of such natural extracts as additives in fruits could delay the growth of mesophilic bacteria. The characterization and identification of the bacterial isolates from fresh-cut apple samples showed that the most prevalent species were Citrobacter freundii, Staphylococcus warneri, Pseudomonas oryzihabitans, Alcalinogenes faecalis, Corynebacterium jeikeium, Micrococcus spp., Pantoea aglomerans and Bacillus spp. Furthermore, an increase in the microbial diversity during the storage time at refrigerated temperatures was observed, except for the sample treated with olive pomace extract and packaged in OPP film. The highest microbial diversity was found for samples with ascorbic acid as an additive. This could indicate a negative effect of ascorbic acid on the microbial inhibition of apple slices. The natural olive pomace extract demonstrated potential as an antimicrobial additive for fresh-cut apples.application/pdfpt_PTMicrobiota assessment of fresh-cut apples packaged in two different filmsMadureira, JoanaGonçalves, SaraSantos-Buelga, CelestinoMargaça, Fernanda M.A.PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801Cabo Verde, SandraHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.3390/microorganisms110511572019-10-28T11:05:30Z20232023-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/19701http://purl.org/coar/access_right/c_abf2open accessMinimally processed appleNatural extractsPackagingNatural microbiota1012317 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/fd0f171e-fa48-4a31-95f9-ecb8d4137689/downloadMicroorganisms
spellingShingle Microbiota assessment of fresh-cut apples packaged in two different films
Madureira, Joana
Minimally processed apple
Natural extracts
Packaging
Natural microbiota
status SINGLETON
subject.fl_str_mv Minimally processed apple
Natural extracts
Packaging
Natural microbiota
title Microbiota assessment of fresh-cut apples packaged in two different films
title_full Microbiota assessment of fresh-cut apples packaged in two different films
title_fullStr Microbiota assessment of fresh-cut apples packaged in two different films
title_full_unstemmed Microbiota assessment of fresh-cut apples packaged in two different films
title_short Microbiota assessment of fresh-cut apples packaged in two different films
title_sort Microbiota assessment of fresh-cut apples packaged in two different films
topic Minimally processed apple
Natural extracts
Packaging
Natural microbiota
topic_facet Minimally processed apple
Natural extracts
Packaging
Natural microbiota
url http://hdl.handle.net/10198/19701
visible 1