Publicação
Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions
| Resumo: | In folk medicine there is a great variety of plants that are used for as infusions, such as Camellia sinensis and Matricaria recutita. Despite the reported biological activities and health benefits of these infusions, most of the knowledge achieved so far is based on folk tradition passed over several generations, without a sound scientific basis. In Trás-os-Montes region, the dried leaves of the narrow-leafed ash “freixo” (Fraxinus angustifolia) are used for medicinal purposes, particularly against high levels of cholesterol, blood pressure and uric acid. These health benefits are associated with the presence of biologically active compounds such as phenolic compounds, essential oils, and polysaccharides, among others. Although phenolic compounds and essential oils have been studied in detail for more than two decades, the structures of the polysaccharides present in plant infusions and their involvement in the health benefits is still incipient. Therefore, in this work we present a study regarding the structure of the polysaccharides present in the infusions of “freixo” dried leaves. “Freixo” leaves were collected and dried according to the popular tradition and used for the preparation of the infusions. The polysaccharides were recovered by dialysis, fractionated by ethanol precipitation and anion exchange chromatography, yielding fractions with distinct ethanol solubility and uronic acid content. Sugar and linkage analysis evidenced the presence of pectic polysaccharides, frequently reported as immunostimulatory active. The assessment of the biological activity of these fractions is in progress. |
|---|---|
| Autores principais: | Martins, Vitor Manuel Ramalheira |
| Outros Autores: | Coimbra, Manuel A. |
| Assunto: | Polysaccharides Infusions Fraxinus angustifolia |
| Ano: | 2012 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172806493470720 |
|---|---|
| author | Martins, Vitor Manuel Ramalheira |
| author2 | Coimbra, Manuel A. |
| author2_role | author |
| author_facet | Martins, Vitor Manuel Ramalheira Coimbra, Manuel A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Martins, Vitor Manuel Ramalheira\",\"Person.identifier.orcid\":\"0000-0003-4144-898X\"},{\"Person.name\":\"Coimbra, Manuel A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Martins, Vitor Manuel Ramalheira Coimbra, Manuel A. |
| datacite.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2013-02-26T13:36:29Z |
| datacite.date.embargoed.fl_str_mv | 2013-02-26T13:36:29Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Polysaccharides Infusions Fraxinus angustifolia |
| datacite.titles.title.fl_str_mv | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Martins, Vitor Manuel Ramalheira Coimbra, Manuel A. |
| dc.date.Accepted.fl_str_mv | 2012-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2013-02-26T13:36:29Z |
| dc.date.embargoed.fl_str_mv | 2013-02-26T13:36:29Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/8155 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Instituto Politécnico de Bragança |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Polysaccharides Infusions Fraxinus angustifolia |
| dc.title.fl_str_mv | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | In folk medicine there is a great variety of plants that are used for as infusions, such as Camellia sinensis and Matricaria recutita. Despite the reported biological activities and health benefits of these infusions, most of the knowledge achieved so far is based on folk tradition passed over several generations, without a sound scientific basis. In Trás-os-Montes region, the dried leaves of the narrow-leafed ash “freixo” (Fraxinus angustifolia) are used for medicinal purposes, particularly against high levels of cholesterol, blood pressure and uric acid. These health benefits are associated with the presence of biologically active compounds such as phenolic compounds, essential oils, and polysaccharides, among others. Although phenolic compounds and essential oils have been studied in detail for more than two decades, the structures of the polysaccharides present in plant infusions and their involvement in the health benefits is still incipient. Therefore, in this work we present a study regarding the structure of the polysaccharides present in the infusions of “freixo” dried leaves. “Freixo” leaves were collected and dried according to the popular tradition and used for the preparation of the infusions. The polysaccharides were recovered by dialysis, fractionated by ethanol precipitation and anion exchange chromatography, yielding fractions with distinct ethanol solubility and uronic acid content. Sugar and linkage analysis evidenced the presence of pectic polysaccharides, frequently reported as immunostimulatory active. The assessment of the biological activity of these fractions is in progress. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferencePaper |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/d1149de9-16f9-4984-9849-b36fbd5d8435/download |
| id | ipb_c67378ac3f761e4f7345d16bcbe4d62e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/8155 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/8155 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Martins, Vitor Manuel Ramalheira Martins, Vitor Manuel Ramalheira https://www.ciencia-id.pt/7C1B-3C44-BDC5 7C1B-3C44-BDC5 http://orcid.org/0000-0003-4144-898X 0000-0003-4144-898X Coimbra, Manuel A. |
| publishDate | 2012 |
| publisher.none.fl_str_mv | Instituto Politécnico de Bragança |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engInstituto Politécnico de BragançaporIn folk medicine there is a great variety of plants that are used for as infusions, such as Camellia sinensis and Matricaria recutita. Despite the reported biological activities and health benefits of these infusions, most of the knowledge achieved so far is based on folk tradition passed over several generations, without a sound scientific basis. In Trás-os-Montes region, the dried leaves of the narrow-leafed ash “freixo” (Fraxinus angustifolia) are used for medicinal purposes, particularly against high levels of cholesterol, blood pressure and uric acid. These health benefits are associated with the presence of biologically active compounds such as phenolic compounds, essential oils, and polysaccharides, among others. Although phenolic compounds and essential oils have been studied in detail for more than two decades, the structures of the polysaccharides present in plant infusions and their involvement in the health benefits is still incipient. Therefore, in this work we present a study regarding the structure of the polysaccharides present in the infusions of “freixo” dried leaves. “Freixo” leaves were collected and dried according to the popular tradition and used for the preparation of the infusions. The polysaccharides were recovered by dialysis, fractionated by ethanol precipitation and anion exchange chromatography, yielding fractions with distinct ethanol solubility and uronic acid content. Sugar and linkage analysis evidenced the presence of pectic polysaccharides, frequently reported as immunostimulatory active. The assessment of the biological activity of these fractions is in progress.application/pdfporIsolation and characterization of polysaccharides from Fraxinus angustifolia infusionsPersonalMartins, Vitor Manuel RamalheiraDSpacehttp://dspace.org/items/ba9148ec-024a-478f-8498-fb3b59950cc7DSpacehttp://dspace.org/items/ba9148ec-024a-478f-8498-fb3b59950cc7MartinsVitor Manuel RamalheiraCiência IDhttps://www.ciencia-id.pt7C1B-3C44-BDC5ORCIDhttp://orcid.org0000-0003-4144-898XScopus Author IDhttps://www.scopus.com57191364121Coimbra, Manuel A.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-972-745-141-82013-02-26T13:36:29Z20122012-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/8155http://purl.org/coar/access_right/c_abf2open accessPolysaccharidesInfusionsFraxinus angustifolia190183 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/d1149de9-16f9-4984-9849-b36fbd5d8435/download11º Encontro de Química dos AlimentosBragança, Portugal |
| spellingShingle | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions Martins, Vitor Manuel Ramalheira Polysaccharides Infusions Fraxinus angustifolia |
| status | SINGLETON |
| subject.fl_str_mv | Polysaccharides Infusions Fraxinus angustifolia |
| title | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| title_full | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| title_fullStr | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| title_full_unstemmed | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| title_short | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| title_sort | Isolation and characterization of polysaccharides from Fraxinus angustifolia infusions |
| topic | Polysaccharides Infusions Fraxinus angustifolia |
| topic_facet | Polysaccharides Infusions Fraxinus angustifolia |
| url | http://hdl.handle.net/10198/8155 |
| visible | 1 |