Publicação
Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water
| Resumo: | Chestnut Ink Disease (CID) is a destructive and a lethal disease of sweet chestnut (Castanea sativa Mill.) that is associated with different soil borne Phytophthora species. CID is very difficult to control because cultural and chemical measures provide limited effects and didn´t prevent disease spread. The aim of this study was to validate different methodologies used for Phytophthora detection from soil, symptomatic vegetal tissues and water. Baiting technics bioassays and DNA-PCR assays based on the multicopy ITS region was compared for method validation. Efficiency, specificity and time response span were the parameters evaluated and compared to validate proposed objectives. Baiting bioassays are very useful when the objective is obtaining isolates of different Phytophthora species and DNA-PCR assays are valuable and basic requisites for integrated management programs for evaluating the applied controls measures to minimize impacts and control the disease. |
|---|---|
| Autores principais: | Gouveia, Maria Eugénia |
| Outros Autores: | Moura, Luísa; Machado, Júlia Melo; Coelho, Valentim |
| Assunto: | Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851047469973504 |
|---|---|
| author | Gouveia, Maria Eugénia |
| author2 | Moura, Luísa Machado, Júlia Melo Coelho, Valentim |
| author2_role | author author author |
| author_facet | Gouveia, Maria Eugénia Moura, Luísa Machado, Júlia Melo Coelho, Valentim |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Gouveia, Maria Eugénia\",\"Person.identifier.orcid\":\"0000-0002-2550-9108\"},{\"Person.name\":\"Moura, Luísa\"},{\"Person.name\":\"Machado, Júlia Melo\"},{\"Person.name\":\"Coelho, Valentim\",\"Person.identifier.orcid\":\"0000-0001-5829-7669\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Gouveia, Maria Eugénia Moura, Luísa Machado, Júlia Melo Coelho, Valentim |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-08T09:10:08Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-08T09:10:08Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| datacite.titles.title.fl_str_mv | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Gouveia, Maria Eugénia Moura, Luísa Machado, Júlia Melo Coelho, Valentim |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-08T09:10:08Z |
| dc.date.embargoed.fl_str_mv | 2023-03-08T09:10:08Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/27548 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Portuguesa de Ciências Florestais |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| dc.title.fl_str_mv | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Chestnut Ink Disease (CID) is a destructive and a lethal disease of sweet chestnut (Castanea sativa Mill.) that is associated with different soil borne Phytophthora species. CID is very difficult to control because cultural and chemical measures provide limited effects and didn´t prevent disease spread. The aim of this study was to validate different methodologies used for Phytophthora detection from soil, symptomatic vegetal tissues and water. Baiting technics bioassays and DNA-PCR assays based on the multicopy ITS region was compared for method validation. Efficiency, specificity and time response span were the parameters evaluated and compared to validate proposed objectives. Baiting bioassays are very useful when the objective is obtaining isolates of different Phytophthora species and DNA-PCR assays are valuable and basic requisites for integrated management programs for evaluating the applied controls measures to minimize impacts and control the disease. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/27548 |
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| language | eng |
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| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/27548 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Gouveia, Maria Eugénia Gouveia, Maria Eugénia http://orcid.org/0000-0002-2550-9108 0000-0002-2550-9108 Moura, Luísa Machado, Júlia Melo Coelho, Valentim Coelho, Valentim https://www.ciencia-id.pt/0618-49BC-EACE 0618-49BC-EACE http://orcid.org/0000-0001-5829-7669 0000-0001-5829-7669 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Sociedade Portuguesa de Ciências Florestais |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engSociedade Portuguesa de Ciências Florestaispt_PTChestnut Ink Disease (CID) is a destructive and a lethal disease of sweet chestnut (Castanea sativa Mill.) that is associated with different soil borne Phytophthora species. CID is very difficult to control because cultural and chemical measures provide limited effects and didn´t prevent disease spread. The aim of this study was to validate different methodologies used for Phytophthora detection from soil, symptomatic vegetal tissues and water. Baiting technics bioassays and DNA-PCR assays based on the multicopy ITS region was compared for method validation. Efficiency, specificity and time response span were the parameters evaluated and compared to validate proposed objectives. Baiting bioassays are very useful when the objective is obtaining isolates of different Phytophthora species and DNA-PCR assays are valuable and basic requisites for integrated management programs for evaluating the applied controls measures to minimize impacts and control the disease.application/pdfpt_PTBaiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and waterPersonalGouveia, Maria EugéniaDSpacehttp://dspace.org/items/99e4701b-e3c2-4594-a37f-42bd49821d6eDSpacehttp://dspace.org/items/99e4701b-e3c2-4594-a37f-42bd49821d6eGouveiaMaria EugéniaORCIDhttp://orcid.org0000-0002-2550-9108Moura, LuísaMachado, Júlia MeloPersonalCoelho, ValentimDSpacehttp://dspace.org/items/99a97d58-8b4c-4a49-9855-d334d568b9f3DSpacehttp://dspace.org/items/99a97d58-8b4c-4a49-9855-d334d568b9f3CoelhoValentimCiência IDhttps://www.ciencia-id.pt0618-49BC-EACEORCIDhttp://orcid.org0000-0001-5829-7669Scopus Author IDhttps://www.scopus.com26533798200HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-972-99656-7-82023-03-08T09:10:08Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27548http://purl.org/coar/access_right/c_abf2open accessChestnut Ink DiseaseResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technology491056 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/a5666c41-566f-4b89-ab3c-c0377500da91/download9º Congresso Florestal Nacional: Livro de Resumos240240Funchal |
| spellingShingle | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water Gouveia, Maria Eugénia Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| subject.fl_str_mv | Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| title | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| title_full | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| title_fullStr | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| title_full_unstemmed | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| title_short | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| title_sort | Baiting bioassays and DNA-PCR based methods for detection of Phytophthora species associated with Chestnut Ink Disease from soil, root tissues and water |
| topic | Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| topic_facet | Chestnut Ink Disease Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| url | http://hdl.handle.net/10198/27548 |
| visible | 1 |