Publicação
Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages
| Resumo: | The rapid increase of the world population has promoted a more sustainable and efficient use of natural resources. To achieve complete and proper upcycling of plant crops, it is important to know their potential for industrial exploitation. Cardoon (Cynara cardunculus L.) is a species native to the Mediterranean basin widely used in different sectors, including food and pharmaceuticals. Despite their multiple industrial applications, not all plant tissues have been incorporated into the value chain. Therefore, this work aimed to characterize the phenolic composition and bioactive properties of cardoon blades throughout the phenological growth cycle. In addition to the structural variety of phytochemicals detected in the blade extracts, their antioxidant, anti-inflammatory, anti-proliferative, and antimicrobial properties were also highlighted. While immature material showed higher levels of phenolic compounds and greater potential to inhibit lipid peroxidation, samples at higher development stages had greater anti-proliferative, anti-inflammatory, and antimicrobial potential. These results demonstrate that the growth cycle influences the bioactive potential of cardoon blades and will be useful to establish suitable industrial applications, such as the development of ingredients for functional foods and nutraceuticals, among other products. Abstract: Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1–B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O- caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 μg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 μg/mL for ∆t of 60 and 120 min, respectively) and higher cytotoxic (B8–B13, GI50 between 7.1 and 17 μg/mL), anti-inflammatory (B13, IC50 of 10 μg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others. |
|---|---|
| Autores principais: | Mandim, Filipa |
| Outros Autores: | Petropoulos, Spyridon Α.; Pinela, José; Dias, Maria Inês; Kostić, Marina; Soković, Marina; Ferreira, Isabel C.F.R.; Barros, Lillian; Santos-Buelga, Celestino |
| Assunto: | Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172778676846592 |
|---|---|
| author | Mandim, Filipa |
| author2 | Petropoulos, Spyridon Α. Pinela, José Dias, Maria Inês Kostić, Marina Soković, Marina Ferreira, Isabel C.F.R. Barros, Lillian Santos-Buelga, Celestino |
| author2_role | author author author author author author author author |
| author_facet | Mandim, Filipa Petropoulos, Spyridon Α. Pinela, José Dias, Maria Inês Kostić, Marina Soković, Marina Ferreira, Isabel C.F.R. Barros, Lillian Santos-Buelga, Celestino |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Mandim, Filipa\",\"Person.identifier.orcid\":\"0000-0002-6494-2326\"},{\"Person.name\":\"Petropoulos, Spyridon Α.\"},{\"Person.name\":\"Pinela, José\",\"Person.identifier.orcid\":\"0000-0001-7523-1637\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Kostić, Marina\"},{\"Person.name\":\"Soković, Marina\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Santos-Buelga, Celestino\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Mandim, Filipa Petropoulos, Spyridon Α. Pinela, José Dias, Maria Inês Kostić, Marina Soković, Marina Ferreira, Isabel C.F.R. Barros, Lillian Santos-Buelga, Celestino |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| datacite.titles.title.fl_str_mv | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Mandim, Filipa Petropoulos, Spyridon Α. Pinela, José Dias, Maria Inês Kostić, Marina Soković, Marina Ferreira, Isabel C.F.R. Barros, Lillian Santos-Buelga, Celestino |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/22326 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| dc.title.fl_str_mv | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The rapid increase of the world population has promoted a more sustainable and efficient use of natural resources. To achieve complete and proper upcycling of plant crops, it is important to know their potential for industrial exploitation. Cardoon (Cynara cardunculus L.) is a species native to the Mediterranean basin widely used in different sectors, including food and pharmaceuticals. Despite their multiple industrial applications, not all plant tissues have been incorporated into the value chain. Therefore, this work aimed to characterize the phenolic composition and bioactive properties of cardoon blades throughout the phenological growth cycle. In addition to the structural variety of phytochemicals detected in the blade extracts, their antioxidant, anti-inflammatory, anti-proliferative, and antimicrobial properties were also highlighted. While immature material showed higher levels of phenolic compounds and greater potential to inhibit lipid peroxidation, samples at higher development stages had greater anti-proliferative, anti-inflammatory, and antimicrobial potential. These results demonstrate that the growth cycle influences the bioactive potential of cardoon blades and will be useful to establish suitable industrial applications, such as the development of ingredients for functional foods and nutraceuticals, among other products. Abstract: Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1–B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O- caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 μg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 μg/mL for ∆t of 60 and 120 min, respectively) and higher cytotoxic (B8–B13, GI50 between 7.1 and 17 μg/mL), anti-inflammatory (B13, IC50 of 10 μg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/4c494183-daec-44d8-88bb-7b7176dcad3b/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ipb_ccaa235db26b4f321c63f239fef5eae2 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/22326 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/22326 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Mandim, Filipa Mandim, Filipa https://www.ciencia-id.pt/F318-9049-EB99 F318-9049-EB99 http://orcid.org/0000-0002-6494-2326 0000-0002-6494-2326 Petropoulos, Spyridon Α. Pinela, José Pinela, José https://www.ciencia-id.pt/771C-2B43-B108 771C-2B43-B108 http://orcid.org/0000-0001-7523-1637 0000-0001-7523-1637 Dias, Maria Inês Dias, Maria Inês https://www.ciencia-id.pt/2A13-4BE6-C7CF 2A13-4BE6-C7CF http://orcid.org/0000-0001-8744-7814 0000-0001-8744-7814 Kostić, Marina Soković, Marina Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Santos-Buelga, Celestino |
| publishDate | 2022 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engThe rapid increase of the world population has promoted a more sustainable and efficient use of natural resources. To achieve complete and proper upcycling of plant crops, it is important to know their potential for industrial exploitation. Cardoon (Cynara cardunculus L.) is a species native to the Mediterranean basin widely used in different sectors, including food and pharmaceuticals. Despite their multiple industrial applications, not all plant tissues have been incorporated into the value chain. Therefore, this work aimed to characterize the phenolic composition and bioactive properties of cardoon blades throughout the phenological growth cycle. In addition to the structural variety of phytochemicals detected in the blade extracts, their antioxidant, anti-inflammatory, anti-proliferative, and antimicrobial properties were also highlighted. While immature material showed higher levels of phenolic compounds and greater potential to inhibit lipid peroxidation, samples at higher development stages had greater anti-proliferative, anti-inflammatory, and antimicrobial potential. These results demonstrate that the growth cycle influences the bioactive potential of cardoon blades and will be useful to establish suitable industrial applications, such as the development of ingredients for functional foods and nutraceuticals, among other products. Abstract: Cardoon (Cynara cardunculus var. altilis) blades were collected at sixteen sampling dates (B1–B16) to study the influence of the phenological growth stage on the phenolic composition and biological properties. Twenty phenolic compounds were identified, among which trans 3,4-O-dicaffeoylquinic acid, 5-O- caffeoylquinic acid, and luteolin-O-hexoside (39.6, 42.6, and 101.0 mg/g extract, respectively) were the main compounds. Immature blades (B3) had a higher content of phenolic compounds (178 mg/g extract) and a greater ability to inhibit the formation of thiobarbituric acid reactive substances (IC50 of 1.61 μg/mL). Samples at more advanced growth stages revealed a greater capacity to inhibit oxidative hemolysis (B8, IC50 of 25 and 47.4 μg/mL for ∆t of 60 and 120 min, respectively) and higher cytotoxic (B8–B13, GI50 between 7.1 and 17 μg/mL), anti-inflammatory (B13, IC50 of 10 μg/mL), and antibacterial activities. In turn, the antifungal activity varied depending on the tested fungi. All these results suggest that maturity influences the phenolic composition and bioactive properties of cardoon blades, which reveal great potential for the development of bioactive ingredients for food and pharmaceutical applications, among others.application/pdfen_ENPhenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stagesPersonalMandim, FilipaDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdMandimFilipaCiência IDhttps://www.ciencia-id.ptF318-9049-EB99ORCIDhttp://orcid.org0000-0002-6494-2326Petropoulos, Spyridon Α.PersonalPinela, JoséDSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4DSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4PinelaJoséCiência IDhttps://www.ciencia-id.pt771C-2B43-B108ORCIDhttp://orcid.org0000-0001-7523-1637Researcher IDhttps://www.researcherid.comB-4466-2014Scopus Author IDhttps://www.scopus.com54392272800PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000Kostić, MarinaSoković, MarinaPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801Santos-Buelga, CelestinoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.3390/biology110506992018-01-19T10:00:00Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/22326http://purl.org/coar/access_right/c_abf2open accessCardoon bladesPhenolic compoundsPhenological growth stageAntioxidant activityTumor cell growth inhibitionAntibacterial/antifungal activitySustainable ingredients631838 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaIntegrated strategies for the development of a novel functional beverage with sustainable production systemsCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/4c494183-daec-44d8-88bb-7b7176dcad3b/download |
| spellingShingle | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages Mandim, Filipa Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| status | SINGLETON |
| subject.fl_str_mv | Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| title | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| title_full | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| title_fullStr | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| title_full_unstemmed | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| title_short | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| title_sort | Phenolic composition and antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities of cardoon blades at different growth stages |
| topic | Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| topic_facet | Cardoon blades Phenolic compounds Phenological growth stage Antioxidant activity Tumor cell growth inhibition Antibacterial/antifungal activity Sustainable ingredients |
| url | http://hdl.handle.net/10198/22326 |
| visible | 1 |