Publicação
Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility
| Resumo: | The incorporation of bioactive natural compounds into biomedical applications offers a promising route to enhance therapeutic efficacy while supporting sustainability. In this study, we investigated the synergistic potential of Sericin, a silk-derived biopolymer, and Chelidonium majus L. (C. majus), a medicinal plant with a diverse phenolic profile, in relation to biological activities relevant for wound care and infection control. A combined experimental strategy was applied, integrating detailed chemical characterization of C. majus extracts with antimicrobial and cytocompatibility assays across different Sericin–plant extract ratios (1:1, 1:2, 2:2, and 2:1). Phytochemical analysis identified and quantified 57 phenolic compounds, including high levels of flavonoids (quercetin, kaempferol, isorhamnetin) and phenolic acids (caffeic and ferulic acid). Salicylic acid (123.6 µg/g), feruloyltyramine (111.8 µg/g), and pinocembrin (98.4 µg/g) were particularly abundant, compounds previously reported to disrupt microbial membranes and impair bacterial viability. These metabolites correlated with the strong antimicrobial activity of C. majus against Gram-positive strains (MIC = 5–10 mg/mL). In combination with Sericin, antimicrobial performance was ratio-dependent, with higher proportions of C. majus (2:1) retaining partial inhibitory effects. Cytocompatibility assays with HFF1 fibroblasts demonstrated low antiproliferative activity across most formulations (GI50 > 400 µg/mL), supporting their potential safety in topical applications. Collectively, the results indicate a concentration-dependent interaction between C. majus phenolics and the Sericin protein matrix, reinforcing their suitability as candidates for natural-based wound healing materials. Importantly, the valorization of Sericin, an underutilized byproduct of the silk industry, together with a widely accessible medicinal plant, underscores the ecological and economic sustainability of this approach. Overall, this work supports the exploration of the development of biomaterials with potential for advancing tissue repair and wound management. |
|---|---|
| Autores principais: | Borges, Ana Margarida |
| Outros Autores: | Ordóñez-Díaz, José Luis; Aquino, Yara; Moreno-Rojas, José M.; Calvo, María Luisa Martín; Vaz, Josiana A.; Calhelha, Ricardo C. |
| Assunto: | Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1868201041811472384 |
|---|---|
| author | Borges, Ana Margarida |
| author2 | Ordóñez-Díaz, José Luis Aquino, Yara Moreno-Rojas, José M. Calvo, María Luisa Martín Vaz, Josiana A. Calhelha, Ricardo C. |
| author2_role | author author author author author author |
| author_facet | Borges, Ana Margarida Ordóñez-Díaz, José Luis Aquino, Yara Moreno-Rojas, José M. Calvo, María Luisa Martín Vaz, Josiana A. Calhelha, Ricardo C. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Borges, Ana Margarida\",\"Person.identifier.orcid\":\"0009-0009-8809-0541\"},{\"Person.name\":\"Ordóñez-Díaz, José Luis\"},{\"Person.name\":\"Aquino, Yara\"},{\"Person.name\":\"Moreno-Rojas, José M.\"},{\"Person.name\":\"Calvo, María Luisa Martín\"},{\"Person.name\":\"Vaz, Josiana A.\",\"Person.identifier.orcid\":\"0000-0002-6989-1169\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Borges, Ana Margarida Ordóñez-Díaz, José Luis Aquino, Yara Moreno-Rojas, José M. Calvo, María Luisa Martín Vaz, Josiana A. Calhelha, Ricardo C. |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-06-12T11:12:38Z |
| datacite.date.embargoed.fl_str_mv | 2026-06-12T11:12:38Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| datacite.titles.title.fl_str_mv | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Borges, Ana Margarida Ordóñez-Díaz, José Luis Aquino, Yara Moreno-Rojas, José M. Calvo, María Luisa Martín Vaz, Josiana A. Calhelha, Ricardo C. |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-06-12T11:12:38Z |
| dc.date.embargoed.fl_str_mv | 2026-06-12T11:12:38Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/36866 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| dc.title.fl_str_mv | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | The incorporation of bioactive natural compounds into biomedical applications offers a promising route to enhance therapeutic efficacy while supporting sustainability. In this study, we investigated the synergistic potential of Sericin, a silk-derived biopolymer, and Chelidonium majus L. (C. majus), a medicinal plant with a diverse phenolic profile, in relation to biological activities relevant for wound care and infection control. A combined experimental strategy was applied, integrating detailed chemical characterization of C. majus extracts with antimicrobial and cytocompatibility assays across different Sericin–plant extract ratios (1:1, 1:2, 2:2, and 2:1). Phytochemical analysis identified and quantified 57 phenolic compounds, including high levels of flavonoids (quercetin, kaempferol, isorhamnetin) and phenolic acids (caffeic and ferulic acid). Salicylic acid (123.6 µg/g), feruloyltyramine (111.8 µg/g), and pinocembrin (98.4 µg/g) were particularly abundant, compounds previously reported to disrupt microbial membranes and impair bacterial viability. These metabolites correlated with the strong antimicrobial activity of C. majus against Gram-positive strains (MIC = 5–10 mg/mL). In combination with Sericin, antimicrobial performance was ratio-dependent, with higher proportions of C. majus (2:1) retaining partial inhibitory effects. Cytocompatibility assays with HFF1 fibroblasts demonstrated low antiproliferative activity across most formulations (GI50 > 400 µg/mL), supporting their potential safety in topical applications. Collectively, the results indicate a concentration-dependent interaction between C. majus phenolics and the Sericin protein matrix, reinforcing their suitability as candidates for natural-based wound healing materials. Importantly, the valorization of Sericin, an underutilized byproduct of the silk industry, together with a widely accessible medicinal plant, underscores the ecological and economic sustainability of this approach. Overall, this work supports the exploration of the development of biomaterials with potential for advancing tissue repair and wound management. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/5983394a-3c19-43a3-a835-f7fe14feb676/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | UIDB/00690/2020 LA/P/0007/2020 |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID |
| funding_str_mv | UIDB/00690/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PT LA/P/0007/2020 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PT |
| id | ipb_cd7e4e5db6afca8cbcc40a226317174b |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/36866 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/36866 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Borges, Ana Margarida Borges, Ana Margarida https://www.ciencia-id.pt/CA13-636E-7820 CA13-636E-7820 http://orcid.org/0009-0009-8809-0541 0009-0009-8809-0541 Ordóñez-Díaz, José Luis Aquino, Yara Aquino, Yara https://www.ciencia-id.pt/3B13-FC91-7BFC 3B13-FC91-7BFC Moreno-Rojas, José M. Calvo, María Luisa Martín Vaz, Josiana A. Vaz, Josiana A. https://www.ciencia-id.pt/171B-88BA-64F5 171B-88BA-64F5 http://orcid.org/0000-0002-6989-1169 0000-0002-6989-1169 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engMDPIengThe incorporation of bioactive natural compounds into biomedical applications offers a promising route to enhance therapeutic efficacy while supporting sustainability. In this study, we investigated the synergistic potential of Sericin, a silk-derived biopolymer, and Chelidonium majus L. (C. majus), a medicinal plant with a diverse phenolic profile, in relation to biological activities relevant for wound care and infection control. A combined experimental strategy was applied, integrating detailed chemical characterization of C. majus extracts with antimicrobial and cytocompatibility assays across different Sericin–plant extract ratios (1:1, 1:2, 2:2, and 2:1). Phytochemical analysis identified and quantified 57 phenolic compounds, including high levels of flavonoids (quercetin, kaempferol, isorhamnetin) and phenolic acids (caffeic and ferulic acid). Salicylic acid (123.6 µg/g), feruloyltyramine (111.8 µg/g), and pinocembrin (98.4 µg/g) were particularly abundant, compounds previously reported to disrupt microbial membranes and impair bacterial viability. These metabolites correlated with the strong antimicrobial activity of C. majus against Gram-positive strains (MIC = 5–10 mg/mL). In combination with Sericin, antimicrobial performance was ratio-dependent, with higher proportions of C. majus (2:1) retaining partial inhibitory effects. Cytocompatibility assays with HFF1 fibroblasts demonstrated low antiproliferative activity across most formulations (GI50 > 400 µg/mL), supporting their potential safety in topical applications. Collectively, the results indicate a concentration-dependent interaction between C. majus phenolics and the Sericin protein matrix, reinforcing their suitability as candidates for natural-based wound healing materials. Importantly, the valorization of Sericin, an underutilized byproduct of the silk industry, together with a widely accessible medicinal plant, underscores the ecological and economic sustainability of this approach. Overall, this work supports the exploration of the development of biomaterials with potential for advancing tissue repair and wound management.application/pdfengAnalysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and CytocompatibilityPersonalBorges, Ana MargaridaDSpacehttp://dspace.org/items/85ec5de9-4999-4dda-9787-fe10250a6af5DSpacehttp://dspace.org/items/85ec5de9-4999-4dda-9787-fe10250a6af5BorgesAna MargaridaCiência IDhttps://www.ciencia-id.ptCA13-636E-7820ORCIDhttp://orcid.org0009-0009-8809-0541Ordóñez-Díaz, José LuisPersonalAquino, YaraDSpacehttp://dspace.org/items/b2b94f0f-fb84-4491-b56d-531f07f4bd77DSpacehttp://dspace.org/items/b2b94f0f-fb84-4491-b56d-531f07f4bd77AquinoYaraCiência IDhttps://www.ciencia-id.pt3B13-FC91-7BFCMoreno-Rojas, José M.Calvo, María Luisa MartínPersonalVaz, Josiana A.DSpacehttp://dspace.org/items/1c1a2cb9-5a36-47ba-bca0-ffcbef28df18DSpacehttp://dspace.org/items/1c1a2cb9-5a36-47ba-bca0-ffcbef28df18VazJosiana A.Ciência IDhttps://www.ciencia-id.pt171B-88BA-64F5ORCIDhttp://orcid.org0000-0002-6989-1169Scopus Author IDhttps://www.scopus.com16305323200PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1661-6596DOIIsPartOf10.3390/ijms262099112026-06-12T11:12:38Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/36866http://purl.org/coar/access_right/c_abf2open accessSustainable healthcareBiomaterialSilkGreater celandinePhenolic compoundsHPLC-MS/MSAntimicrobialCytocompatibility31129440 bytesFundação para a Ciência e a TecnologiaMountain Research Center - UIDB/00690/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00690%2F2020/PTUIDB/00690/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regions - LA/P/0007/2020info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0007%2F2020/PTLA/P/0007/20206817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/5983394a-3c19-43a3-a835-f7fe14feb676/downloadInternational Journal of Molecular Sciences2620123 |
| spellingShingle | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility Borges, Ana Margarida Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| status | SINGLETON |
| subject.fl_str_mv | Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| title | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| title_full | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| title_fullStr | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| title_full_unstemmed | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| title_short | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| title_sort | Analysis of the Phenolic Profile of Chelidonium majus L. and Its Combination with Sericin: Balancing Antimicrobial Activity and Cytocompatibility |
| topic | Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| topic_facet | Sustainable healthcare Biomaterial Silk Greater celandine Phenolic compounds HPLC-MS/MS Antimicrobial Cytocompatibility |
| url | http://hdl.handle.net/10198/36866 |
| visible | 1 |
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