Publicação
Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers
| Resumo: | Hibiscus sabdariffa L. is an herbaceous medicinal plantused as infusion for the treatment of several disorders, namely, fever and high blood pressure, gastrointestinal and hepatic diseases. In addition to those health benefits, the calyces area potential source of natural pigments, due to its high content in anthocyanins. This work aimed to optimize the experimental conditions of anthocyanins’extraction from dried calyces of H. sabdariffa, comparing two methodologies, maceration and ultrasound assisted extraction, in order to develop a natural colorant. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology (RSM) was applied using the circumscribed central composite design of three variables with five levels. Time, temperature and ethanol - water proportion - in the case of maceration, were the relevant independent variables, while for ultrasound assisted extraction, the ultrasonic power was used instead of temperature which was kept constant. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS: delphinidin3-sambubioside and cyanidin-3-sambubioside. The responses used as criteria were the quantification of the identified anthocyanins and the extraction yield of the final residue. Ultrasound assisted extraction proved to be the most effective method: 43 min, 390 W and 46 % of ethanol. At these optimal conditions, the extraction yield was 61.2%, with a total anthocyanins’ content of 23.1 mg/g dried plant, and 47.5 mg/g extract. Additionally, the response values were slightly improved by studying the solid/liquid ratio effect using the optimal conditions in dose-response format (5 to 200 g/L), showing continuously decreasing values as the solid/liquid ratio increases. Overall, the obtained results showed the possibility of using H. sabdariffa flowers as a source of anthocyanin compounds for industrial applications. |
|---|---|
| Autores principais: | Jabeur, Inès |
| Outros Autores: | Pereira, Eliana; Prieto Lage, Miguel A.; Antonio, Amilcar L.; Barros, Lillian; Ferreira, Isabel C.F.R. |
| Assunto: | Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173343465046016 |
|---|---|
| author | Jabeur, Inès |
| author2 | Pereira, Eliana Prieto Lage, Miguel A. Antonio, Amilcar L. Barros, Lillian Ferreira, Isabel C.F.R. |
| author2_role | author author author author author |
| author_facet | Jabeur, Inès Pereira, Eliana Prieto Lage, Miguel A. Antonio, Amilcar L. Barros, Lillian Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Jabeur, Inès\",\"Person.identifier.orcid\":\"0000-0002-7485-3554\"},{\"Person.name\":\"Pereira, Eliana\",\"Person.identifier.orcid\":\"0000-0003-2854-6745\"},{\"Person.name\":\"Prieto Lage, Miguel A.\",\"Person.identifier.orcid\":\"0000-0002-3513-0054\"},{\"Person.name\":\"Antonio, Amilcar L.\",\"Person.identifier.orcid\":\"0000-0001-8271-9964\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Jabeur, Inès Pereira, Eliana Prieto Lage, Miguel A. Antonio, Amilcar L. Barros, Lillian Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-10-17T15:37:03Z |
| datacite.date.embargoed.fl_str_mv | 2018-10-17T15:37:03Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| datacite.titles.title.fl_str_mv | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Jabeur, Inès Pereira, Eliana Prieto Lage, Miguel A. Antonio, Amilcar L. Barros, Lillian Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-10-17T15:37:03Z |
| dc.date.embargoed.fl_str_mv | 2018-10-17T15:37:03Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/18088 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| dc.title.fl_str_mv | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Hibiscus sabdariffa L. is an herbaceous medicinal plantused as infusion for the treatment of several disorders, namely, fever and high blood pressure, gastrointestinal and hepatic diseases. In addition to those health benefits, the calyces area potential source of natural pigments, due to its high content in anthocyanins. This work aimed to optimize the experimental conditions of anthocyanins’extraction from dried calyces of H. sabdariffa, comparing two methodologies, maceration and ultrasound assisted extraction, in order to develop a natural colorant. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology (RSM) was applied using the circumscribed central composite design of three variables with five levels. Time, temperature and ethanol - water proportion - in the case of maceration, were the relevant independent variables, while for ultrasound assisted extraction, the ultrasonic power was used instead of temperature which was kept constant. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS: delphinidin3-sambubioside and cyanidin-3-sambubioside. The responses used as criteria were the quantification of the identified anthocyanins and the extraction yield of the final residue. Ultrasound assisted extraction proved to be the most effective method: 43 min, 390 W and 46 % of ethanol. At these optimal conditions, the extraction yield was 61.2%, with a total anthocyanins’ content of 23.1 mg/g dried plant, and 47.5 mg/g extract. Additionally, the response values were slightly improved by studying the solid/liquid ratio effect using the optimal conditions in dose-response format (5 to 200 g/L), showing continuously decreasing values as the solid/liquid ratio increases. Overall, the obtained results showed the possibility of using H. sabdariffa flowers as a source of anthocyanin compounds for industrial applications. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/18088 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| person_str_mv | Jabeur, Inès Jabeur, Inès http://orcid.org/0000-0002-7485-3554 0000-0002-7485-3554 Pereira, Eliana Pereira, Eliana https://www.ciencia-id.pt/BE17-462D-9A75 BE17-462D-9A75 http://orcid.org/0000-0003-2854-6745 0000-0003-2854-6745 Prieto Lage, Miguel A. Prieto Lage, Miguel A. https://www.ciencia-id.pt/221E-1E00-AB74 221E-1E00-AB74 http://orcid.org/0000-0002-3513-0054 0000-0002-3513-0054 Antonio, Amilcar L. Antonio, Amilcar L. https://www.ciencia-id.pt/1014-5259-DE86 1014-5259-DE86 http://orcid.org/0000-0001-8271-9964 0000-0001-8271-9964 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2018 |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engpt_PTHibiscus sabdariffa L. is an herbaceous medicinal plantused as infusion for the treatment of several disorders, namely, fever and high blood pressure, gastrointestinal and hepatic diseases. In addition to those health benefits, the calyces area potential source of natural pigments, due to its high content in anthocyanins. This work aimed to optimize the experimental conditions of anthocyanins’extraction from dried calyces of H. sabdariffa, comparing two methodologies, maceration and ultrasound assisted extraction, in order to develop a natural colorant. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology (RSM) was applied using the circumscribed central composite design of three variables with five levels. Time, temperature and ethanol - water proportion - in the case of maceration, were the relevant independent variables, while for ultrasound assisted extraction, the ultrasonic power was used instead of temperature which was kept constant. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS: delphinidin3-sambubioside and cyanidin-3-sambubioside. The responses used as criteria were the quantification of the identified anthocyanins and the extraction yield of the final residue. Ultrasound assisted extraction proved to be the most effective method: 43 min, 390 W and 46 % of ethanol. At these optimal conditions, the extraction yield was 61.2%, with a total anthocyanins’ content of 23.1 mg/g dried plant, and 47.5 mg/g extract. Additionally, the response values were slightly improved by studying the solid/liquid ratio effect using the optimal conditions in dose-response format (5 to 200 g/L), showing continuously decreasing values as the solid/liquid ratio increases. Overall, the obtained results showed the possibility of using H. sabdariffa flowers as a source of anthocyanin compounds for industrial applications.application/pdfpt_PTOptimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowersPersonalJabeur, InèsDSpacehttp://dspace.org/items/f3e4dc3b-31d6-4c54-9161-7236ea5c8dceDSpacehttp://dspace.org/items/f3e4dc3b-31d6-4c54-9161-7236ea5c8dceJabeurInèsORCIDhttp://orcid.org0000-0002-7485-3554PersonalPereira, ElianaDSpacehttp://dspace.org/items/dda60cdc-0a2a-4c10-863e-eaef1e6f1fb9DSpacehttp://dspace.org/items/dda60cdc-0a2a-4c10-863e-eaef1e6f1fb9PereiraElianaCiência IDhttps://www.ciencia-id.ptBE17-462D-9A75ORCIDhttp://orcid.org0000-0003-2854-6745Researcher IDhttps://www.researcherid.comQ-8017-2018Scopus Author IDhttps://www.scopus.com55938912300PersonalPrieto Lage, Miguel A.DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579PrietoMiguel A.Ciência IDhttps://www.ciencia-id.pt221E-1E00-AB74ORCIDhttp://orcid.org0000-0002-3513-0054Researcher IDhttps://www.researcherid.comG-4516-2011Scopus Author IDhttps://www.scopus.com35937495700Scopus Author IDhttps://www.scopus.com57207755963PersonalAntonio, Amilcar L.DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28AntonioAmilcar L.Ciência IDhttps://www.ciencia-id.pt1014-5259-DE86ORCIDhttp://orcid.org0000-0001-8271-9964Researcher IDhttps://www.researcherid.comJ-1960-2014Scopus Author IDhttps://www.scopus.com44560921900PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-99976-718-5-12018-10-17T15:37:03Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/18088http://purl.org/coar/access_right/c_abf2open accessHibiscus sabdariffa L. flowersAnthocyaninsHeat and ultrasound assisted extractionsResponse surface methodology933653 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference object2018http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/829e583e-fa5f-4ee7-b0f0-4dfd298926e3/downloadIX International Scientific Agriculture Symposium “AGROSYM 2018”11771177Bósnia-Herzegovina |
| spellingShingle | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers Jabeur, Inès Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| status | SINGLETON |
| subject.fl_str_mv | Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| title | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| title_full | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| title_fullStr | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| title_full_unstemmed | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| title_short | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| title_sort | Optimization and comparison of maceration and ultrassound assisted extraction systems for the production of a colorant extract from Hibiscus sabdariffa L. flowers |
| topic | Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| topic_facet | Hibiscus sabdariffa L. flowers Anthocyanins Heat and ultrasound assisted extractions Response surface methodology |
| url | http://hdl.handle.net/10198/18088 |
| visible | 1 |