Publicação
Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials
| Resumo: | Phlomis crinita Cav. (P. crinita), known as " Khayat el-djerah " in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative and three Gram-positive clinical bacterial strains, as well as in vitro antioxidant activity of hydroethanolic extracts from leaves (HLE), flowers (HFE), and rhizomes (HRE) of P. crinita. Fifteen phenolic compounds such as four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, and four benzoic acid derivatives were identified for the first time in P. crinita by HPLC-DAD, with quantitative differences among the analyzed parts. HRE exhibited high levels of total phenolics (262.97 ± 16.2 μg GAE/mg DW) and flavonoids (71.87 ± 3.25 μg QE/mg DW), while HLE had the highest flavonols content (18.89 ± 5.12 μg QE/mg DW). All extracts demonstrated strong antioxidant properties. HLE exhibited the highest potency, with IC50 values of 15.46 ± 0.45 µg/mL (DPPH) and 11.71 ± 0.50 µg/mL (ABTS). HLE exhibited good reducing power (FRAP A0.5 = 40.07 ± 2.82 µg/mL), while HRE showed the best reducing power (Phenanthroline A0.5 = 7.88 ± 1.63 µg/mL). All extracts revealed broad-spectrum antibacterial effects, and HRE exhibited the most potent activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL. These results showed that P. crinita could be useful as source of bioactive compounds for pharmaceutical and food industry. |
|---|---|
| Autores principais: | Chelgham, Abdelhakim |
| Outros Autores: | Saadi, Abdelkader; Merouane, Abdelaziz; Bensouıcı, Chawki; Cakmak, Yavuz Selim; Pires, Tânia C.S.P. |
| Assunto: | Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| Ano: | 2025 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173394334613504 |
|---|---|
| author | Chelgham, Abdelhakim |
| author2 | Saadi, Abdelkader Merouane, Abdelaziz Bensouıcı, Chawki Cakmak, Yavuz Selim Pires, Tânia C.S.P. |
| author2_role | author author author author author |
| author_facet | Chelgham, Abdelhakim Saadi, Abdelkader Merouane, Abdelaziz Bensouıcı, Chawki Cakmak, Yavuz Selim Pires, Tânia C.S.P. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Chelgham, Abdelhakim\"},{\"Person.name\":\"Saadi, Abdelkader\"},{\"Person.name\":\"Merouane, Abdelaziz\"},{\"Person.name\":\"Bensouıcı, Chawki\"},{\"Person.name\":\"Cakmak, Yavuz Selim\"},{\"Person.name\":\"Pires, Tânia C.S.P.\",\"Person.identifier.orcid\":\"0000-0002-3954-3833\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Chelgham, Abdelhakim Saadi, Abdelkader Merouane, Abdelaziz Bensouıcı, Chawki Cakmak, Yavuz Selim Pires, Tânia C.S.P. |
| datacite.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2026-02-11T16:24:10Z |
| datacite.date.embargoed.fl_str_mv | 2026-02-11T16:24:10Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| datacite.titles.title.fl_str_mv | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Chelgham, Abdelhakim Saadi, Abdelkader Merouane, Abdelaziz Bensouıcı, Chawki Cakmak, Yavuz Selim Pires, Tânia C.S.P. |
| dc.date.Accepted.fl_str_mv | 2025-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2026-02-11T16:24:10Z |
| dc.date.embargoed.fl_str_mv | 2026-02-11T16:24:10Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/35719 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Pamukkale University |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| dc.title.fl_str_mv | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Phlomis crinita Cav. (P. crinita), known as " Khayat el-djerah " in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative and three Gram-positive clinical bacterial strains, as well as in vitro antioxidant activity of hydroethanolic extracts from leaves (HLE), flowers (HFE), and rhizomes (HRE) of P. crinita. Fifteen phenolic compounds such as four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, and four benzoic acid derivatives were identified for the first time in P. crinita by HPLC-DAD, with quantitative differences among the analyzed parts. HRE exhibited high levels of total phenolics (262.97 ± 16.2 μg GAE/mg DW) and flavonoids (71.87 ± 3.25 μg QE/mg DW), while HLE had the highest flavonols content (18.89 ± 5.12 μg QE/mg DW). All extracts demonstrated strong antioxidant properties. HLE exhibited the highest potency, with IC50 values of 15.46 ± 0.45 µg/mL (DPPH) and 11.71 ± 0.50 µg/mL (ABTS). HLE exhibited good reducing power (FRAP A0.5 = 40.07 ± 2.82 µg/mL), while HRE showed the best reducing power (Phenanthroline A0.5 = 7.88 ± 1.63 µg/mL). All extracts revealed broad-spectrum antibacterial effects, and HRE exhibited the most potent activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL. These results showed that P. crinita could be useful as source of bioactive compounds for pharmaceutical and food industry. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/60a67338-b395-4a11-9e8c-5e531c3c41d3/download |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/35719 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/35719 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Chelgham, Abdelhakim Saadi, Abdelkader Merouane, Abdelaziz Bensouıcı, Chawki Cakmak, Yavuz Selim Pires, Tânia C.S.P. Pires, Tânia C.S.P. https://www.ciencia-id.pt/321E-2D96-00CA 321E-2D96-00CA http://orcid.org/0000-0002-3954-3833 0000-0002-3954-3833 |
| publishDate | 2025 |
| publisher.none.fl_str_mv | Pamukkale University |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engPamukkale UniversityporPhlomis crinita Cav. (P. crinita), known as " Khayat el-djerah " in Algerian folk medicine, is used for wound healing and abdominal pain relief. This study assessed the phytochemical profile, phenolic content, antimicrobial activity against five Gram-negative and three Gram-positive clinical bacterial strains, as well as in vitro antioxidant activity of hydroethanolic extracts from leaves (HLE), flowers (HFE), and rhizomes (HRE) of P. crinita. Fifteen phenolic compounds such as four flavonoids, trans-cinnamic acid, six cinnamic acid derivatives, and four benzoic acid derivatives were identified for the first time in P. crinita by HPLC-DAD, with quantitative differences among the analyzed parts. HRE exhibited high levels of total phenolics (262.97 ± 16.2 μg GAE/mg DW) and flavonoids (71.87 ± 3.25 μg QE/mg DW), while HLE had the highest flavonols content (18.89 ± 5.12 μg QE/mg DW). All extracts demonstrated strong antioxidant properties. HLE exhibited the highest potency, with IC50 values of 15.46 ± 0.45 µg/mL (DPPH) and 11.71 ± 0.50 µg/mL (ABTS). HLE exhibited good reducing power (FRAP A0.5 = 40.07 ± 2.82 µg/mL), while HRE showed the best reducing power (Phenanthroline A0.5 = 7.88 ± 1.63 µg/mL). All extracts revealed broad-spectrum antibacterial effects, and HRE exhibited the most potent activity against Enterococcus faecalis, with a minimum inhibitory concentration (MIC) value of 1.25 mg/mL. These results showed that P. crinita could be useful as source of bioactive compounds for pharmaceutical and food industry.application/pdfExploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentialsChelgham, AbdelhakimSaadi, AbdelkaderMerouane, AbdelazizBensouıcı, ChawkiCakmak, Yavuz SelimPersonalPires, Tânia C.S.P.DSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fDSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fPiresTânia C.S.P.Ciência IDhttps://www.ciencia-id.pt321E-2D96-00CAORCIDhttp://orcid.org0000-0002-3954-3833Researcher IDhttps://www.researcherid.comQ-2842-2018Scopus Author IDhttps://www.scopus.com57057440000HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.21448/ijsm.14666892026-02-11T16:24:10Z20252025-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/35719http://purl.org/coar/access_right/c_abf2open accessPhlomis crinitaHerbal medicineHPLC-DADPhenolic compoundsBiological activity666549 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2025http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/60a67338-b395-4a11-9e8c-5e531c3c41d3/downloadInternational Journal of Secondary Metabolite122381396 |
| spellingShingle | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials Chelgham, Abdelhakim Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| status | SINGLETON |
| subject.fl_str_mv | Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| title | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| title_full | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| title_fullStr | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| title_full_unstemmed | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| title_short | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| title_sort | Exploring Phlomis crinita extracts: HPLC analysis, phenolic content, antioxidant and antimicrobial potentials |
| topic | Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| topic_facet | Phlomis crinita Herbal medicine HPLC-DAD Phenolic compounds Biological activity |
| url | http://hdl.handle.net/10198/35719 |
| visible | 1 |