Publicação
Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples
| Resumo: | Irradiation is recognized as a safe and effective food preservation method used worldwide to decontaminate and extend the shelf-life of fresh and dried agricultural products [1]. Tuberaria lignosa (Sweet) Samp. is one of the most popular medicinal plants in several regions of the Iberian Peninsula, used to treat various diseases and ailments [2]. Interesting antioxidant properties were already highlighted by our research group in this plant [3], which may be related to beneficial effects in human health by neutralizing free radicals that arise from various metabolic processes. In the present work, the effects of gamma irradiation (doses of 0, 1, 5 and 10 kGy performed in a Co-60 experimental equipment) and extraction method (water infusion or decoction) on the antioxidant activity of wild and commercial dried samples of T. lignosa were evaluated and compared. Four in vitro assays were used to evaluate the antioxidant activity, measuring DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of thiobarbituric acid reactive substances (TBARS) formation. The potential effects of irradiation dose, extraction method and plant origin were evaluated by discriminant analysis, using the stepwise procedure. The results showed that the different irradiation dose does not have significant effects on the antioxidant activity of the samples. Regarding the extraction method, decoctions showed higher antioxidant activity than infusions (lower EC50values) in all in vitro assays, probably due to a higher extraction yield of bioactive compounds. Additionally, the origin of the plant (wild or commercial) also had no effect on the analyzed parameters. The results herein reported support the traditional use of T. lignosa in folk medicine (especially decoction preparations), and also the use of gamma-irradiation as an effective technology to process this plant in order to maintain antioxidant potential. |
|---|---|
| Autores principais: | Pinela, José |
| Outros Autores: | Antonio, Amilcar L.; Cabo Verde, Sandra; Barreira, João C.M.; Carvalho, Ana Maria; Oliveira, Beatriz; Ferreira, Isabel C.F.R. |
| Assunto: | Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850889363587072 |
|---|---|
| author | Pinela, José |
| author2 | Antonio, Amilcar L. Cabo Verde, Sandra Barreira, João C.M. Carvalho, Ana Maria Oliveira, Beatriz Ferreira, Isabel C.F.R. |
| author2_role | author author author author author author |
| author_facet | Pinela, José Antonio, Amilcar L. Cabo Verde, Sandra Barreira, João C.M. Carvalho, Ana Maria Oliveira, Beatriz Ferreira, Isabel C.F.R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Pinela, José\",\"Person.identifier.orcid\":\"0000-0001-7523-1637\"},{\"Person.name\":\"Antonio, Amilcar L.\",\"Person.identifier.orcid\":\"0000-0001-8271-9964\"},{\"Person.name\":\"Cabo Verde, Sandra\"},{\"Person.name\":\"Barreira, João C.M.\",\"Person.identifier.orcid\":\"0000-0003-1233-0990\"},{\"Person.name\":\"Carvalho, Ana Maria\",\"Person.identifier.orcid\":\"0000-0001-5508-5935\"},{\"Person.name\":\"Oliveira, Beatriz\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pinela, José Antonio, Amilcar L. Cabo Verde, Sandra Barreira, João C.M. Carvalho, Ana Maria Oliveira, Beatriz Ferreira, Isabel C.F.R. |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2015-02-24T09:41:12Z |
| datacite.date.embargoed.fl_str_mv | 2015-02-24T09:41:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| datacite.titles.title.fl_str_mv | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pinela, José Antonio, Amilcar L. Cabo Verde, Sandra Barreira, João C.M. Carvalho, Ana Maria Oliveira, Beatriz Ferreira, Isabel C.F.R. |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2015-02-24T09:41:12Z |
| dc.date.embargoed.fl_str_mv | 2015-02-24T09:41:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/11687 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | IAEA - International Atomic Energy Agency |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| dc.title.fl_str_mv | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Irradiation is recognized as a safe and effective food preservation method used worldwide to decontaminate and extend the shelf-life of fresh and dried agricultural products [1]. Tuberaria lignosa (Sweet) Samp. is one of the most popular medicinal plants in several regions of the Iberian Peninsula, used to treat various diseases and ailments [2]. Interesting antioxidant properties were already highlighted by our research group in this plant [3], which may be related to beneficial effects in human health by neutralizing free radicals that arise from various metabolic processes. In the present work, the effects of gamma irradiation (doses of 0, 1, 5 and 10 kGy performed in a Co-60 experimental equipment) and extraction method (water infusion or decoction) on the antioxidant activity of wild and commercial dried samples of T. lignosa were evaluated and compared. Four in vitro assays were used to evaluate the antioxidant activity, measuring DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of thiobarbituric acid reactive substances (TBARS) formation. The potential effects of irradiation dose, extraction method and plant origin were evaluated by discriminant analysis, using the stepwise procedure. The results showed that the different irradiation dose does not have significant effects on the antioxidant activity of the samples. Regarding the extraction method, decoctions showed higher antioxidant activity than infusions (lower EC50values) in all in vitro assays, probably due to a higher extraction yield of bioactive compounds. Additionally, the origin of the plant (wild or commercial) also had no effect on the analyzed parameters. The results herein reported support the traditional use of T. lignosa in folk medicine (especially decoction preparations), and also the use of gamma-irradiation as an effective technology to process this plant in order to maintain antioxidant potential. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/4d9cfb9f-a65b-4dd5-855c-7e69339032d7/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID |
| id | ipb_d1e10a508e0ca151ca7edb1b59a6bc45 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/11687 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/11687 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pinela, José Pinela, José https://www.ciencia-id.pt/771C-2B43-B108 771C-2B43-B108 http://orcid.org/0000-0001-7523-1637 0000-0001-7523-1637 Antonio, Amilcar L. Antonio, Amilcar L. https://www.ciencia-id.pt/1014-5259-DE86 1014-5259-DE86 http://orcid.org/0000-0001-8271-9964 0000-0001-8271-9964 Cabo Verde, Sandra Barreira, João C.M. Barreira, João C.M. http://orcid.org/0000-0003-1233-0990 0000-0003-1233-0990 Carvalho, Ana Maria Carvalho, Ana Maria https://www.ciencia-id.pt/D31A-35AF-E2A9 D31A-35AF-E2A9 http://orcid.org/0000-0001-5508-5935 0000-0001-5508-5935 Oliveira, Beatriz Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 |
| publishDate | 2014 |
| publisher.none.fl_str_mv | IAEA - International Atomic Energy Agency |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engIAEA - International Atomic Energy AgencyporIrradiation is recognized as a safe and effective food preservation method used worldwide to decontaminate and extend the shelf-life of fresh and dried agricultural products [1]. Tuberaria lignosa (Sweet) Samp. is one of the most popular medicinal plants in several regions of the Iberian Peninsula, used to treat various diseases and ailments [2]. Interesting antioxidant properties were already highlighted by our research group in this plant [3], which may be related to beneficial effects in human health by neutralizing free radicals that arise from various metabolic processes. In the present work, the effects of gamma irradiation (doses of 0, 1, 5 and 10 kGy performed in a Co-60 experimental equipment) and extraction method (water infusion or decoction) on the antioxidant activity of wild and commercial dried samples of T. lignosa were evaluated and compared. Four in vitro assays were used to evaluate the antioxidant activity, measuring DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, reducing power, inhibition of β-carotene bleaching and inhibition of thiobarbituric acid reactive substances (TBARS) formation. The potential effects of irradiation dose, extraction method and plant origin were evaluated by discriminant analysis, using the stepwise procedure. The results showed that the different irradiation dose does not have significant effects on the antioxidant activity of the samples. Regarding the extraction method, decoctions showed higher antioxidant activity than infusions (lower EC50values) in all in vitro assays, probably due to a higher extraction yield of bioactive compounds. Additionally, the origin of the plant (wild or commercial) also had no effect on the analyzed parameters. The results herein reported support the traditional use of T. lignosa in folk medicine (especially decoction preparations), and also the use of gamma-irradiation as an effective technology to process this plant in order to maintain antioxidant potential.application/pdfporEffects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samplesPersonalPinela, JoséDSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4DSpacehttp://dspace.org/items/706cd9ff-065b-4c98-98a7-5c0a9e45adc4PinelaJoséCiência IDhttps://www.ciencia-id.pt771C-2B43-B108ORCIDhttp://orcid.org0000-0001-7523-1637Researcher IDhttps://www.researcherid.comB-4466-2014Scopus Author IDhttps://www.scopus.com54392272800PersonalAntonio, Amilcar L.DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28DSpacehttp://dspace.org/items/4b23e496-8d11-4ff5-a8ec-e1c2235e5a28AntonioAmilcar L.Ciência IDhttps://www.ciencia-id.pt1014-5259-DE86ORCIDhttp://orcid.org0000-0001-8271-9964Researcher IDhttps://www.researcherid.comJ-1960-2014Scopus Author IDhttps://www.scopus.com44560921900Cabo Verde, SandraPersonalBarreira, João C.M.DSpacehttp://dspace.org/items/4629b12c-39b0-4da8-8b8d-6efba5cf2d81DSpacehttp://dspace.org/items/4629b12c-39b0-4da8-8b8d-6efba5cf2d81BarreiraJoão C.M.ORCIDhttp://orcid.org0000-0003-1233-0990Researcher IDhttps://www.researcherid.comD-8269-2013Scopus Author IDhttps://www.scopus.com54895546900PersonalCarvalho, Ana MariaDSpacehttp://dspace.org/items/977f5254-f7c5-4c91-873f-2700ecd4692fDSpacehttp://dspace.org/items/977f5254-f7c5-4c91-873f-2700ecd4692fCarvalhoAna MariaCiência IDhttps://www.ciencia-id.ptD31A-35AF-E2A9ORCIDhttp://orcid.org0000-0001-5508-5935Scopus Author IDhttps://www.scopus.com20336503900Oliveira, BeatrizPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2015-02-24T09:41:12Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/11687http://purl.org/coar/access_right/c_abf2open accessTuberaria lignosaGamma irradiationExtraction methodsAntioxidant potential614707 bytesFundação para a Ciência e a TecnologiaStrategic Project - UI 690 - 2011-20126817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaRECOVERY OF THE SUSTAINABLE USE OF TRADITIONAL FOODS THROUGH EMERGING PRESERVATION TECHNOLOGIESCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaBIOACTIVE PROPERTIES & CYTOPROTECTIVE POTENTIAL OF NATURAL EXTRACTS/INDIVIDUAL COMPOUNDS: APPLICATION OF SINGLE CELL GEL ELECTROPHORESIS AND OTHER BIOCHEMICAL, CHEMICAL AND ELECTROCHEMICAL ASSAYSCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/4d9cfb9f-a65b-4dd5-855c-7e69339032d7/downloadInternational Symposium on Food Safety and Quality: Applications of Nuclear and Related TechniquesViena, Áustria |
| spellingShingle | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples Pinela, José Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| subject.fl_str_mv | Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| title | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| title_full | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| title_fullStr | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| title_full_unstemmed | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| title_short | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| title_sort | Effects of gamma irradiation and extraction method in the antioxidant potential of wild and commercial Tuberaria lignosa samples |
| topic | Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| topic_facet | Tuberaria lignosa Gamma irradiation Extraction methods Antioxidant potential |
| url | http://hdl.handle.net/10198/11687 |
| visible | 1 |