Publicação
Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques
| Resumo: | In the present work, the extraction and detection of DNA along a complete industrial soybean oil processing chain was described to monitor the presence of Roundup Ready (RR) soybean. The analysed samples comprised all the steps prior to industrial oil extraction, namely, raw, cracked, laminated and expanded seeds, and the defatted flour as a sub-product. The samples collected at the refining unit included the crude oil, degummed/neutralised, washed, bleached and deodorised oil, as final product. The amplification of soybean lectin gene by end-point polymerase chain reaction (PCR) was successfully achieved in all the steps of extraction and refining processes, until the fully refined soybean oil. The amplification of RR soybean by PCR assays using event-specific primers was also achieved for all the extraction and refining steps, except for the intermediate steps of refining (neutralisation, washing and bleaching) possibly due to sample instability. The real-time PCR assays using specific probes confirmed all the results and proved that it is possible to detect and quantify genetically modified organisms in the fully refined soybean oil. To our knowledge, this has never been reported before and represents an important accomplishment regarding the traceability of genetically modified organisms in refined oils. |
|---|---|
| Autores principais: | Costa, Joana |
| Outros Autores: | Mafra, Isabel; Amaral, Joana S.; Oliveira, Beatriz |
| Assunto: | GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso restrito |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173293355696128 |
|---|---|
| author | Costa, Joana |
| author2 | Mafra, Isabel Amaral, Joana S. Oliveira, Beatriz |
| author2_role | author author author |
| author_facet | Costa, Joana Mafra, Isabel Amaral, Joana S. Oliveira, Beatriz |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Costa, Joana\"},{\"Person.name\":\"Mafra, Isabel\"},{\"Person.name\":\"Amaral, Joana S.\",\"Person.identifier.orcid\":\"0000-0002-3648-7303\"},{\"Person.name\":\"Oliveira, Beatriz\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Costa, Joana Mafra, Isabel Amaral, Joana S. Oliveira, Beatriz |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-06-07T09:24:28Z |
| datacite.date.embargoed.fl_str_mv | 2011-06-07T09:24:28Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| datacite.subjects.subject.fl_str_mv | GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| datacite.titles.title.fl_str_mv | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Costa, Joana Mafra, Isabel Amaral, Joana S. Oliveira, Beatriz |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-06-07T09:24:28Z |
| dc.date.embargoed.fl_str_mv | 2011-06-07T09:24:28Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/5015 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Food Research International |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_16ec |
| dc.subject.none.fl_str_mv | GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| dc.title.fl_str_mv | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | In the present work, the extraction and detection of DNA along a complete industrial soybean oil processing chain was described to monitor the presence of Roundup Ready (RR) soybean. The analysed samples comprised all the steps prior to industrial oil extraction, namely, raw, cracked, laminated and expanded seeds, and the defatted flour as a sub-product. The samples collected at the refining unit included the crude oil, degummed/neutralised, washed, bleached and deodorised oil, as final product. The amplification of soybean lectin gene by end-point polymerase chain reaction (PCR) was successfully achieved in all the steps of extraction and refining processes, until the fully refined soybean oil. The amplification of RR soybean by PCR assays using event-specific primers was also achieved for all the extraction and refining steps, except for the intermediate steps of refining (neutralisation, washing and bleaching) possibly due to sample instability. The real-time PCR assays using specific probes confirmed all the results and proved that it is possible to detect and quantify genetically modified organisms in the fully refined soybean oil. To our knowledge, this has never been reported before and represents an important accomplishment regarding the traceability of genetically modified organisms in refined oils. |
| dirty | 0 |
| eu_rights_str_mv | restrictedAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/a0097e83-0317-4ea5-8f09-1785193803da/download |
| id | ipb_dbb7d2b89aaaecc88ec445a24f6e4c97 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/5015 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/5015 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Costa, Joana Mafra, Isabel Amaral, Joana S. Amaral, Joana S. https://www.ciencia-id.pt/5319-7DE8-BEDA 5319-7DE8-BEDA http://orcid.org/0000-0002-3648-7303 0000-0002-3648-7303 Oliveira, Beatriz |
| publishDate | 2010 |
| publisher.none.fl_str_mv | Food Research International |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engFood Research InternationalporIn the present work, the extraction and detection of DNA along a complete industrial soybean oil processing chain was described to monitor the presence of Roundup Ready (RR) soybean. The analysed samples comprised all the steps prior to industrial oil extraction, namely, raw, cracked, laminated and expanded seeds, and the defatted flour as a sub-product. The samples collected at the refining unit included the crude oil, degummed/neutralised, washed, bleached and deodorised oil, as final product. The amplification of soybean lectin gene by end-point polymerase chain reaction (PCR) was successfully achieved in all the steps of extraction and refining processes, until the fully refined soybean oil. The amplification of RR soybean by PCR assays using event-specific primers was also achieved for all the extraction and refining steps, except for the intermediate steps of refining (neutralisation, washing and bleaching) possibly due to sample instability. The real-time PCR assays using specific probes confirmed all the results and proved that it is possible to detect and quantify genetically modified organisms in the fully refined soybean oil. To our knowledge, this has never been reported before and represents an important accomplishment regarding the traceability of genetically modified organisms in refined oils.application/pdfporMonitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniquesCosta, JoanaMafra, IsabelPersonalAmaral, Joana S.DSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdDSpacehttp://dspace.org/items/42be2cf4-adc4-4e7f-ac60-7aab515b38cdAmaralJoana S.Ciência IDhttps://www.ciencia-id.pt5319-7DE8-BEDAORCIDhttp://orcid.org0000-0002-3648-7303Oliveira, BeatrizHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0963-9969DOIIsPartOf10.1016/j.foodres.2009.10.0032011-06-07T09:24:28Z20102010-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/5015http://purl.org/coar/access_right/c_16ecrestricted accessGMO detectionSoybean oilIndustrial refiningDNA extractionReal-time PCR311597 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_16ecapplication/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/a0097e83-0317-4ea5-8f09-1785193803da/downloadFood Research International301306 |
| spellingShingle | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques Costa, Joana GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| status | SINGLETON |
| subject.fl_str_mv | GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| title | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| title_full | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| title_fullStr | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| title_full_unstemmed | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| title_short | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| title_sort | Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques |
| topic | GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| topic_facet | GMO detection Soybean oil Industrial refining DNA extraction Real-time PCR |
| url | http://hdl.handle.net/10198/5015 |
| visible | 1 |