Publicação
Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight
| Resumo: | Cryphonectria parasitica, introduced in the NE of Portugal since 1989, is now an important and widespread pathogen of chestnut (Castanea sativa Mill.) which causes losses in chestnut fruit production and led to decline and death of many chestnut trees. Hypovirulence, a virus mediated attenuation of fungal pathogenesis, has not been frequent until now in Portugal but recently some cases of healing cankers were reported by chestnut growers. We assessed these sites and one of them, where healing cankers were clustered, was intensively surveyed. From all the trees with healing cankers, white and orange phenotypes of C. parasitica were isolated, which are both of the same vc type (EU-11). White isolates of C. parasitica showed reduced pigmentation in colony colour which was not completely stable, suppressed conidiation, reduction of phenol oxidase but no significant differences in lesion length and sporulation on chestnut twigs on dormant chestnut trees. Hypovirulent strains have dsRNA virus in the cytoplasm which is the typical element that reduces virulence in C. parasitica. Bands of dsRNA were identified in all of the white isolates and some variation in band dimension and number of bands was detected. The L-dsRNA (12 kbp), which is the diagnostic fragment of C. parasitica infection, was detected. The presence of white phenotypes of infected C. parasitica (CHV) and the fact that spreads occur in natural conditions is promising for its development as a biological control agent of Chestnut Blight. |
|---|---|
| Autores principais: | Gouveia, Maria Eugénia |
| Outros Autores: | Coelho, Valentim; Monteiro, Maria do Loreto |
| Assunto: | Hypovirulence Biological control Castanea sativa |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | comunicação em conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851463268106240 |
|---|---|
| author | Gouveia, Maria Eugénia |
| author2 | Coelho, Valentim Monteiro, Maria do Loreto |
| author2_role | author author |
| author_facet | Gouveia, Maria Eugénia Coelho, Valentim Monteiro, Maria do Loreto |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Gouveia, Maria Eugénia\",\"Person.identifier.orcid\":\"0000-0002-2550-9108\"},{\"Person.name\":\"Coelho, Valentim\",\"Person.identifier.orcid\":\"0000-0001-5829-7669\"},{\"Person.name\":\"Monteiro, Maria do Loreto\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Gouveia, Maria Eugénia Coelho, Valentim Monteiro, Maria do Loreto |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-05-16T09:53:43Z |
| datacite.date.embargoed.fl_str_mv | 2011-05-16T09:53:43Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Hypovirulence Biological control Castanea sativa |
| datacite.titles.title.fl_str_mv | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Gouveia, Maria Eugénia Coelho, Valentim Monteiro, Maria do Loreto |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-05-16T09:53:43Z |
| dc.date.embargoed.fl_str_mv | 2011-05-16T09:53:43Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/4291 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | ISHS |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Hypovirulence Biological control Castanea sativa |
| dc.title.fl_str_mv | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_5794 |
| description | Cryphonectria parasitica, introduced in the NE of Portugal since 1989, is now an important and widespread pathogen of chestnut (Castanea sativa Mill.) which causes losses in chestnut fruit production and led to decline and death of many chestnut trees. Hypovirulence, a virus mediated attenuation of fungal pathogenesis, has not been frequent until now in Portugal but recently some cases of healing cankers were reported by chestnut growers. We assessed these sites and one of them, where healing cankers were clustered, was intensively surveyed. From all the trees with healing cankers, white and orange phenotypes of C. parasitica were isolated, which are both of the same vc type (EU-11). White isolates of C. parasitica showed reduced pigmentation in colony colour which was not completely stable, suppressed conidiation, reduction of phenol oxidase but no significant differences in lesion length and sporulation on chestnut twigs on dormant chestnut trees. Hypovirulent strains have dsRNA virus in the cytoplasm which is the typical element that reduces virulence in C. parasitica. Bands of dsRNA were identified in all of the white isolates and some variation in band dimension and number of bands was detected. The L-dsRNA (12 kbp), which is the diagnostic fragment of C. parasitica infection, was detected. The presence of white phenotypes of infected C. parasitica (CHV) and the fact that spreads occur in natural conditions is promising for its development as a biological control agent of Chestnut Blight. |
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| eu_rights_str_mv | openAccess |
| format | conferencePaper |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/4291 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
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| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/4291 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Gouveia, Maria Eugénia Gouveia, Maria Eugénia http://orcid.org/0000-0002-2550-9108 0000-0002-2550-9108 Coelho, Valentim Coelho, Valentim https://www.ciencia-id.pt/0618-49BC-EACE 0618-49BC-EACE http://orcid.org/0000-0001-5829-7669 0000-0001-5829-7669 Monteiro, Maria do Loreto |
| publishDate | 2010 |
| publisher.none.fl_str_mv | ISHS |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engISHSporCryphonectria parasitica, introduced in the NE of Portugal since 1989, is now an important and widespread pathogen of chestnut (Castanea sativa Mill.) which causes losses in chestnut fruit production and led to decline and death of many chestnut trees. Hypovirulence, a virus mediated attenuation of fungal pathogenesis, has not been frequent until now in Portugal but recently some cases of healing cankers were reported by chestnut growers. We assessed these sites and one of them, where healing cankers were clustered, was intensively surveyed. From all the trees with healing cankers, white and orange phenotypes of C. parasitica were isolated, which are both of the same vc type (EU-11). White isolates of C. parasitica showed reduced pigmentation in colony colour which was not completely stable, suppressed conidiation, reduction of phenol oxidase but no significant differences in lesion length and sporulation on chestnut twigs on dormant chestnut trees. Hypovirulent strains have dsRNA virus in the cytoplasm which is the typical element that reduces virulence in C. parasitica. Bands of dsRNA were identified in all of the white isolates and some variation in band dimension and number of bands was detected. The L-dsRNA (12 kbp), which is the diagnostic fragment of C. parasitica infection, was detected. The presence of white phenotypes of infected C. parasitica (CHV) and the fact that spreads occur in natural conditions is promising for its development as a biological control agent of Chestnut Blight.application/pdfporPotential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blightPersonalGouveia, Maria EugéniaDSpacehttp://dspace.org/items/99e4701b-e3c2-4594-a37f-42bd49821d6eDSpacehttp://dspace.org/items/99e4701b-e3c2-4594-a37f-42bd49821d6eGouveiaMaria EugéniaORCIDhttp://orcid.org0000-0002-2550-9108PersonalCoelho, ValentimDSpacehttp://dspace.org/items/99a97d58-8b4c-4a49-9855-d334d568b9f3DSpacehttp://dspace.org/items/99a97d58-8b4c-4a49-9855-d334d568b9f3CoelhoValentimCiência IDhttps://www.ciencia-id.pt0618-49BC-EACEORCIDhttp://orcid.org0000-0001-5829-7669Scopus Author IDhttps://www.scopus.com26533798200Monteiro, Maria do LoretoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0567-7572DOIIsPartOf10.17660/ActaHortic.2010.866.592011-05-16T09:53:43Z20102010-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/4291http://purl.org/coar/access_right/c_abf2open accessHypovirulenceBiological controlCastanea sativa72228 bytesother research producthttp://purl.org/coar/resource_type/c_5794conference paperhttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/e5e75ced-0cc5-4b7e-97a9-3c3b4e734150/downloadActa Horticulturae, 866443448 |
| spellingShingle | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight Gouveia, Maria Eugénia Hypovirulence Biological control Castanea sativa |
| subject.fl_str_mv | Hypovirulence Biological control Castanea sativa |
| title | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| title_full | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| title_fullStr | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| title_full_unstemmed | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| title_short | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| title_sort | Potential of local hypovirulent strains of Cryphonectria parasitica for biological control of chestnut blight |
| topic | Hypovirulence Biological control Castanea sativa |
| topic_facet | Hypovirulence Biological control Castanea sativa |
| url | http://hdl.handle.net/10198/4291 |
| visible | 1 |