Publicação
Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils
| Resumo: | One of the biggest threats for the chestnut tree in Portugal and in Europe is the ink disease. The disease is caused by two Phytophthora species (Phytophthora cinnamomi and Phytophthora cambivora). These root parasites have biological and epidemiological characteristics that provide them with rapid adaptation to the environmental conditions and have a high capacity for survival. With this work we intend to develop molecular methods for the detection and identification of Phytophthora in chestnut soils, providing greater sensitivity of detection and greater specificity of identification. In this study, two methods were compared for the detection of Phytophthora from soils infected with chestnut ink disease (natural soil from a stand with diseased chestnut trees and potting soil from greenhouses). In the classical method (baiting method), for the detection of Phytophthora, leaf discs of chestnut (Castanea sativa) were used and subsequent identification of isolates. For molecular methods, soil DNA was extracted from the DNeasy® PowerSoil® Pro Kit (Qiagen) and was amplified from the ITS region of ribosomal DNA (rDNA) using the universal primers ITS4 and ITS6. It was possible to obtain 67% of positive detection from natural soils by baiting method and 75% of positive detection from soil of pots from greenhouse. P. cinnamomi was the species identified from the baiting method and the nucleotide identity of the ITS region of the three identified isolates ranged between 98 and 100%. Trap tissue assays are very useful when the aim is to obtain isolates of different Phytophthora species and DNA-PCR assays are valuable and basic requirements for integrated management programs to evaluate the control measures applied to minimize impacts and control the disease. |
|---|---|
| Autores principais: | Machado, Júlia Melo |
| Assunto: | Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | dissertação de mestrado |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | português |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850728245690368 |
|---|---|
| author | Machado, Júlia Melo |
| author_facet | Machado, Júlia Melo |
| author_role | author |
| contributor_name_str_mv | Gouveia, Maria Eugénia Coelho, Valentim Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Machado, Júlia Melo\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Gouveia, Maria Eugénia Coelho, Valentim Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Machado, Júlia Melo |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-01-11T15:04:15Z |
| datacite.date.embargoed.fl_str_mv | 2023-01-11T15:04:15Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| datacite.titles.title.fl_str_mv | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| dc.contributor.none.fl_str_mv | Gouveia, Maria Eugénia Coelho, Valentim Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Machado, Júlia Melo |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-01-11T15:04:15Z |
| dc.date.embargoed.fl_str_mv | 2023-01-11T15:04:15Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/26454 |
| dc.language.none.fl_str_mv | por |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| dc.title.fl_str_mv | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_bdcc |
| description | One of the biggest threats for the chestnut tree in Portugal and in Europe is the ink disease. The disease is caused by two Phytophthora species (Phytophthora cinnamomi and Phytophthora cambivora). These root parasites have biological and epidemiological characteristics that provide them with rapid adaptation to the environmental conditions and have a high capacity for survival. With this work we intend to develop molecular methods for the detection and identification of Phytophthora in chestnut soils, providing greater sensitivity of detection and greater specificity of identification. In this study, two methods were compared for the detection of Phytophthora from soils infected with chestnut ink disease (natural soil from a stand with diseased chestnut trees and potting soil from greenhouses). In the classical method (baiting method), for the detection of Phytophthora, leaf discs of chestnut (Castanea sativa) were used and subsequent identification of isolates. For molecular methods, soil DNA was extracted from the DNeasy® PowerSoil® Pro Kit (Qiagen) and was amplified from the ITS region of ribosomal DNA (rDNA) using the universal primers ITS4 and ITS6. It was possible to obtain 67% of positive detection from natural soils by baiting method and 75% of positive detection from soil of pots from greenhouse. P. cinnamomi was the species identified from the baiting method and the nucleotide identity of the ITS region of the three identified isolates ranged between 98 and 100%. Trap tissue assays are very useful when the aim is to obtain isolates of different Phytophthora species and DNA-PCR assays are valuable and basic requirements for integrated management programs to evaluate the control measures applied to minimize impacts and control the disease. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/63a85cf4-98ed-4b3e-94c0-650972d1627c/download |
| id | ipb_defcefcf4fef2a472ef869bb28137b25 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/26454 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | por |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/26454 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Machado, Júlia Melo |
| publishDate | 2022 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | porpt_PTOne of the biggest threats for the chestnut tree in Portugal and in Europe is the ink disease. The disease is caused by two Phytophthora species (Phytophthora cinnamomi and Phytophthora cambivora). These root parasites have biological and epidemiological characteristics that provide them with rapid adaptation to the environmental conditions and have a high capacity for survival. With this work we intend to develop molecular methods for the detection and identification of Phytophthora in chestnut soils, providing greater sensitivity of detection and greater specificity of identification. In this study, two methods were compared for the detection of Phytophthora from soils infected with chestnut ink disease (natural soil from a stand with diseased chestnut trees and potting soil from greenhouses). In the classical method (baiting method), for the detection of Phytophthora, leaf discs of chestnut (Castanea sativa) were used and subsequent identification of isolates. For molecular methods, soil DNA was extracted from the DNeasy® PowerSoil® Pro Kit (Qiagen) and was amplified from the ITS region of ribosomal DNA (rDNA) using the universal primers ITS4 and ITS6. It was possible to obtain 67% of positive detection from natural soils by baiting method and 75% of positive detection from soil of pots from greenhouse. P. cinnamomi was the species identified from the baiting method and the nucleotide identity of the ITS region of the three identified isolates ranged between 98 and 100%. Trap tissue assays are very useful when the aim is to obtain isolates of different Phytophthora species and DNA-PCR assays are valuable and basic requirements for integrated management programs to evaluate the control measures applied to minimize impacts and control the disease.application/pdfpt_PTDetection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soilsMachado, Júlia MeloGouveia, Maria EugéniaCoelho, ValentimHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptURNurn:tid:2031594382023-01-11T15:04:15Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/26454http://purl.org/coar/access_right/c_abf2open accessInk diseaseChestnut treePhytophthora cinnamomiMolecular biology1100758 bytesliteraturehttp://purl.org/coar/resource_type/c_bdccmaster thesis2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/63a85cf4-98ed-4b3e-94c0-650972d1627c/download |
| spellingShingle | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils Machado, Júlia Melo Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| subject.fl_str_mv | Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| title | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| title_full | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| title_fullStr | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| title_full_unstemmed | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| title_short | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| title_sort | Detection and identification of Phytophthora sp. associated with ink disease of chestnut (Castanea sativa) from natural soils |
| topic | Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| topic_facet | Ink disease Chestnut tree Phytophthora cinnamomi Molecular biology |
| url | http://hdl.handle.net/10198/26454 |
| visible | 1 |