Publicação
Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues
| Resumo: | Although cholesterol is essential for the functioning of the human body, its excess is responsible for causing atherosclerosis, along with several other diseases that affect the bones, liver, hormones, and immune system1. As a natural solution, mushrooms are being studied for their ability to reduce cholesterol due to their richness in molecules capable of reducing both cholesterol absorption and synthesis2,3. Therefore, the present work investigated two mushrooms species (Agaricus bisporus L. and Pleurotus ostreatus L.), aiming at extracting the highest contents of statins and ergosterol. Ultrasound assisted extraction (UAE) optimization was performed followed by quantification of ergosterol and statins of the extracts by HPLC-UV4,5, as well as the effect of the temperature on both extractions (with and without iced bath), since statins are described as sensitive to high temperatures. For A. bisporus, there was no significant differences in the concentration of ergosterol and pravastatin when applying ice bath during the UAE. For P. ostreatus, the ice bath resulted in a 6% decrease in the ergosterol concentration and a 35% increase in pravastatin content, suggesting that the best extraction method to obtain an extract rich in hypocholesterolemic compounds is a cold extraction. For optimization, the P. ostreatus extract revealed the highest amount of pravastatin and ergosterol (63.85 and 23.95 mg/g of extract, respectively), in which the best extraction conditions were 400 W of ultrasonic power, 4 minutes and 44 seconds of extraction time, and 15.2 g/L of solid:liquid ratio, using methanol as solvent, besides using an ultrasound extraction with ice bath. |
|---|---|
| Autores principais: | Ueda, Jonata Massao |
| Outros Autores: | Benhassen, Yosra; Carocho, Márcio; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina A. |
| Assunto: | Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851300836343808 |
|---|---|
| author | Ueda, Jonata Massao |
| author2 | Benhassen, Yosra Carocho, Márcio Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. |
| author2_role | author author author author author |
| author_facet | Ueda, Jonata Massao Benhassen, Yosra Carocho, Márcio Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Ueda, Jonata Massao\",\"Person.identifier.orcid\":\"0000-0002-1452-3764\"},{\"Person.name\":\"Benhassen, Yosra\"},{\"Person.name\":\"Carocho, Márcio\",\"Person.identifier.orcid\":\"0000-0002-8978-4547\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Calhelha, Ricardo C.\",\"Person.identifier.orcid\":\"0000-0002-6801-4578\"},{\"Person.name\":\"Heleno, Sandrina A.\",\"Person.identifier.orcid\":\"0000-0001-7224-1098\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Ueda, Jonata Massao Benhassen, Yosra Carocho, Márcio Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-15T16:04:32Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-15T16:04:32Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| datacite.titles.title.fl_str_mv | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Ueda, Jonata Massao Benhassen, Yosra Carocho, Márcio Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-15T16:04:32Z |
| dc.date.embargoed.fl_str_mv | 2023-03-15T16:04:32Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/27752 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Portuguesa de Química |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| dc.title.fl_str_mv | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Although cholesterol is essential for the functioning of the human body, its excess is responsible for causing atherosclerosis, along with several other diseases that affect the bones, liver, hormones, and immune system1. As a natural solution, mushrooms are being studied for their ability to reduce cholesterol due to their richness in molecules capable of reducing both cholesterol absorption and synthesis2,3. Therefore, the present work investigated two mushrooms species (Agaricus bisporus L. and Pleurotus ostreatus L.), aiming at extracting the highest contents of statins and ergosterol. Ultrasound assisted extraction (UAE) optimization was performed followed by quantification of ergosterol and statins of the extracts by HPLC-UV4,5, as well as the effect of the temperature on both extractions (with and without iced bath), since statins are described as sensitive to high temperatures. For A. bisporus, there was no significant differences in the concentration of ergosterol and pravastatin when applying ice bath during the UAE. For P. ostreatus, the ice bath resulted in a 6% decrease in the ergosterol concentration and a 35% increase in pravastatin content, suggesting that the best extraction method to obtain an extract rich in hypocholesterolemic compounds is a cold extraction. For optimization, the P. ostreatus extract revealed the highest amount of pravastatin and ergosterol (63.85 and 23.95 mg/g of extract, respectively), in which the best extraction conditions were 400 W of ultrasonic power, 4 minutes and 44 seconds of extraction time, and 15.2 g/L of solid:liquid ratio, using methanol as solvent, besides using an ultrasound extraction with ice bath. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
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| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/7fcfa594-9593-4966-936e-c30f25e0e7b2/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID CEEC IND 2018 CEEC IND 2017 |
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| identifier.url.fl_str_mv | http://hdl.handle.net/10198/27752 |
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| institution | Instituto Politécnico de Bragança |
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| language | eng |
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| person_str_mv | Ueda, Jonata Massao Ueda, Jonata Massao https://www.ciencia-id.pt/4417-F90B-68A3 4417-F90B-68A3 http://orcid.org/0000-0002-1452-3764 0000-0002-1452-3764 Benhassen, Yosra Carocho, Márcio Carocho, Márcio https://www.ciencia-id.pt/5614-005B-5CF1 5614-005B-5CF1 http://orcid.org/0000-0002-8978-4547 0000-0002-8978-4547 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Calhelha, Ricardo C. Calhelha, Ricardo C. https://www.ciencia-id.pt/F313-E3CE-554E F313-E3CE-554E http://orcid.org/0000-0002-6801-4578 0000-0002-6801-4578 Heleno, Sandrina A. Heleno, Sandrina A. https://www.ciencia-id.pt/8B18-3095-6FC9 8B18-3095-6FC9 http://orcid.org/0000-0001-7224-1098 0000-0001-7224-1098 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Sociedade Portuguesa de Química |
| reponame_str | Biblioteca Digital do IPB |
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| spelling | engSociedade Portuguesa de Químicapt_PTAlthough cholesterol is essential for the functioning of the human body, its excess is responsible for causing atherosclerosis, along with several other diseases that affect the bones, liver, hormones, and immune system1. As a natural solution, mushrooms are being studied for their ability to reduce cholesterol due to their richness in molecules capable of reducing both cholesterol absorption and synthesis2,3. Therefore, the present work investigated two mushrooms species (Agaricus bisporus L. and Pleurotus ostreatus L.), aiming at extracting the highest contents of statins and ergosterol. Ultrasound assisted extraction (UAE) optimization was performed followed by quantification of ergosterol and statins of the extracts by HPLC-UV4,5, as well as the effect of the temperature on both extractions (with and without iced bath), since statins are described as sensitive to high temperatures. For A. bisporus, there was no significant differences in the concentration of ergosterol and pravastatin when applying ice bath during the UAE. For P. ostreatus, the ice bath resulted in a 6% decrease in the ergosterol concentration and a 35% increase in pravastatin content, suggesting that the best extraction method to obtain an extract rich in hypocholesterolemic compounds is a cold extraction. For optimization, the P. ostreatus extract revealed the highest amount of pravastatin and ergosterol (63.85 and 23.95 mg/g of extract, respectively), in which the best extraction conditions were 400 W of ultrasonic power, 4 minutes and 44 seconds of extraction time, and 15.2 g/L of solid:liquid ratio, using methanol as solvent, besides using an ultrasound extraction with ice bath.application/pdfpt_PTDevelopment of statin-like and ergosterol enriched extracts from mushrooms bioresiduesPersonalUeda, Jonata MassaoDSpacehttp://dspace.org/items/f98a1e3c-b52e-4997-b835-854c0e2a2c35DSpacehttp://dspace.org/items/f98a1e3c-b52e-4997-b835-854c0e2a2c35UedaJonata MassaoCiência IDhttps://www.ciencia-id.pt4417-F90B-68A3ORCIDhttp://orcid.org0000-0002-1452-3764Scopus Author IDhttps://www.scopus.com57222522617Benhassen, YosraPersonalCarocho, MárcioDSpacehttp://dspace.org/items/46b39e1f-2674-4be1-a96a-c2e5792e6979DSpacehttp://dspace.org/items/46b39e1f-2674-4be1-a96a-c2e5792e6979CarochoMárcioCiência IDhttps://www.ciencia-id.pt5614-005B-5CF1ORCIDhttp://orcid.org0000-0002-8978-4547Scopus Author IDhttps://www.scopus.com52463229800PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801PersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalHeleno, Sandrina A.DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676HelenoSandrina A.Ciência IDhttps://www.ciencia-id.pt8B18-3095-6FC9ORCIDhttp://orcid.org0000-0001-7224-1098Researcher IDhttps://www.researcherid.comL-5951-2014Scopus Author IDhttps://www.scopus.com30067731800HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-989-8124-37-12023-03-15T16:04:32Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27752http://purl.org/coar/access_right/c_abf2open accessErgosterolMushroomResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technology1448076 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNot AvailableCEEC IND 2018Crossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaNot AvailableCEEC IND 2017Crossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/7fcfa594-9593-4966-936e-c30f25e0e7b2/downloadLivro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA7979Aveiro |
| spellingShingle | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues Ueda, Jonata Massao Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| subject.fl_str_mv | Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| title | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| title_full | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| title_fullStr | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| title_full_unstemmed | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| title_short | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| title_sort | Development of statin-like and ergosterol enriched extracts from mushrooms bioresidues |
| topic | Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| topic_facet | Ergosterol Mushroom Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| url | http://hdl.handle.net/10198/27752 |
| visible | 1 |