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Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed

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Resumo:This study aimed to investigate the effect of extraction methods and solvents (Soxhlet and supercritical fluid extraction, SFE) on the chemical profile (gas chromatography [GC]/mass spectrometry and GC/flame ionization detection) and antimicrobial, antioxidant, antitumor, and anti-inflammatory activities of quinoa Piabiru (QP) husk crude extracts. Soxhlet was applied using 100% water (QPSH2O), and ethanolic solutions of 50% ethanol (ethanol:water 50:50 v/v, QPSetOH50), 70% ethanol (QPSetOH70), and 99% ethanol (QPSetOH99) as solvents. SFE was applied using CO2 and n-propane as solvents (QPSF). QPSH2O extract showed a higher concentration of phytosterols (stigmasterol, β-sitosterol, 7,8-epoxylanostan-11-OL,3-acetoxy) and carotenoids (rhodopin) and oleic acid, displaying intermediate thiobarbituric acid reactive substance (TBARS) antioxidant activity. Intermediate concentrations of ethanol (QPSetOH50 and QPSetOH70 extracts) increased the extraction yields and the antibacterial activity of the extracts (Pseudomonas aeruginosa, Salmonella enterica, Bacillus cereus, and Staphylococcus aureus). Higher concentrations of ethanol (QPSetOH99 extract) contributed to increased antioxidant activity as assessed by TBARS and higher recoveries of 4-(allyloxy)-2-methyl-2-pentanol, nonadecane, and lauric, myristic, palmitic, linoleic, stearic, arachidic, behenic, and lignoceric acids. Finally, the QPSF extract presented higher antioxidant activity by DPPH, ABTS, and ferric-reducing antioxidant power, higher content of 5-methoxy-2-pentanone, 5-methoxy-2-methyl- 2-pentanol, 1-(1,3-dimethylbutoxy)-2-propanol, oxalic, undecanoic, myristoleic, tricosanoic, pentadecanoic, elaidic, 11-eicosenoic, and erucic acids, and better antifungal activity against Aspergillus brasiliensis than the other extracts. Crude extracts were not cytotoxic against non-tumor cells (Vero) and did not show antitumor or antiinflammatory activities. Thus, antagonistic or synergistic effects of the phytochemical profile of quinoa husk crude extracts may present potential food and pharmaceutical applications. Practical Applications: The exceptional nutritional properties of quinoa seeds have boosted their cultivation in more than 123 countries. However, quinoa husks are generally considered waste. This study, which is of utmost importance, demonstrates the potential of extracting bioactive compounds from quinoa husks via pressurized fluids, turning them into a health-promoting co-product. This approach could minimize the current shortage of new antibiotics, antifungals, antitumor agents, and anti-inflammatory substances in the pharmaceutical and food sectors. By converting quinoa husks into valuable bioactive extracts,we contribute significantly to developing effective natural compounds, underlining the significance of our collective work.
Autores principais:Casalvara, Rhaira Fernanda Ayoub
Outros Autores:Santos, Everton da Silva; Mattos, Jose Vinicius; Pimentel, Tatiana Colombo; Calhelha, Ricardo C.; Pires, Tânia C.S.P.; Rodrigues, Daniele Bobrowski; Pereira, Carla; Corrêa, Rúbia C.G.; Cardozo‐Filho, Lucio; Gonçalves, José Eduardo
Assunto:Extraction methods Green chemistry Industrial biowaste Phytochemicals Quinoa brasileira
Ano:2024
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Casalvara, Rhaira Fernanda Ayoub
author2 Santos, Everton da Silva
Mattos, Jose Vinicius
Pimentel, Tatiana Colombo
Calhelha, Ricardo C.
Pires, Tânia C.S.P.
Rodrigues, Daniele Bobrowski
Pereira, Carla
Corrêa, Rúbia C.G.
Cardozo‐Filho, Lucio
Gonçalves, José Eduardo
author2_role author
author
author
author
author
author
author
author
author
author
author_facet Casalvara, Rhaira Fernanda Ayoub
Santos, Everton da Silva
Mattos, Jose Vinicius
Pimentel, Tatiana Colombo
Calhelha, Ricardo C.
Pires, Tânia C.S.P.
Rodrigues, Daniele Bobrowski
Pereira, Carla
Corrêa, Rúbia C.G.
Cardozo‐Filho, Lucio
Gonçalves, José Eduardo
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
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datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Casalvara, Rhaira Fernanda Ayoub
Santos, Everton da Silva
Mattos, Jose Vinicius
Pimentel, Tatiana Colombo
Calhelha, Ricardo C.
Pires, Tânia C.S.P.
Rodrigues, Daniele Bobrowski
Pereira, Carla
Corrêa, Rúbia C.G.
Cardozo‐Filho, Lucio
Gonçalves, José Eduardo
datacite.date.Accepted.fl_str_mv 2024-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2024-10-09T09:06:45Z
datacite.date.embargoed.fl_str_mv 2024-10-09T09:06:45Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Extraction methods
Green chemistry
Industrial biowaste
Phytochemicals
Quinoa brasileira
datacite.titles.title.fl_str_mv Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Casalvara, Rhaira Fernanda Ayoub
Santos, Everton da Silva
Mattos, Jose Vinicius
Pimentel, Tatiana Colombo
Calhelha, Ricardo C.
Pires, Tânia C.S.P.
Rodrigues, Daniele Bobrowski
Pereira, Carla
Corrêa, Rúbia C.G.
Cardozo‐Filho, Lucio
Gonçalves, José Eduardo
dc.date.Accepted.fl_str_mv 2024-01-01T00:00:00Z
dc.date.available.fl_str_mv 2024-10-09T09:06:45Z
dc.date.embargoed.fl_str_mv 2024-10-09T09:06:45Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/30392
dc.language.none.fl_str_mv eng
dc.publisher.none.fl_str_mv Wiley
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Extraction methods
Green chemistry
Industrial biowaste
Phytochemicals
Quinoa brasileira
dc.title.fl_str_mv Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_6501
description This study aimed to investigate the effect of extraction methods and solvents (Soxhlet and supercritical fluid extraction, SFE) on the chemical profile (gas chromatography [GC]/mass spectrometry and GC/flame ionization detection) and antimicrobial, antioxidant, antitumor, and anti-inflammatory activities of quinoa Piabiru (QP) husk crude extracts. Soxhlet was applied using 100% water (QPSH2O), and ethanolic solutions of 50% ethanol (ethanol:water 50:50 v/v, QPSetOH50), 70% ethanol (QPSetOH70), and 99% ethanol (QPSetOH99) as solvents. SFE was applied using CO2 and n-propane as solvents (QPSF). QPSH2O extract showed a higher concentration of phytosterols (stigmasterol, β-sitosterol, 7,8-epoxylanostan-11-OL,3-acetoxy) and carotenoids (rhodopin) and oleic acid, displaying intermediate thiobarbituric acid reactive substance (TBARS) antioxidant activity. Intermediate concentrations of ethanol (QPSetOH50 and QPSetOH70 extracts) increased the extraction yields and the antibacterial activity of the extracts (Pseudomonas aeruginosa, Salmonella enterica, Bacillus cereus, and Staphylococcus aureus). Higher concentrations of ethanol (QPSetOH99 extract) contributed to increased antioxidant activity as assessed by TBARS and higher recoveries of 4-(allyloxy)-2-methyl-2-pentanol, nonadecane, and lauric, myristic, palmitic, linoleic, stearic, arachidic, behenic, and lignoceric acids. Finally, the QPSF extract presented higher antioxidant activity by DPPH, ABTS, and ferric-reducing antioxidant power, higher content of 5-methoxy-2-pentanone, 5-methoxy-2-methyl- 2-pentanol, 1-(1,3-dimethylbutoxy)-2-propanol, oxalic, undecanoic, myristoleic, tricosanoic, pentadecanoic, elaidic, 11-eicosenoic, and erucic acids, and better antifungal activity against Aspergillus brasiliensis than the other extracts. Crude extracts were not cytotoxic against non-tumor cells (Vero) and did not show antitumor or antiinflammatory activities. Thus, antagonistic or synergistic effects of the phytochemical profile of quinoa husk crude extracts may present potential food and pharmaceutical applications. Practical Applications: The exceptional nutritional properties of quinoa seeds have boosted their cultivation in more than 123 countries. However, quinoa husks are generally considered waste. This study, which is of utmost importance, demonstrates the potential of extracting bioactive compounds from quinoa husks via pressurized fluids, turning them into a health-promoting co-product. This approach could minimize the current shortage of new antibiotics, antifungals, antitumor agents, and anti-inflammatory substances in the pharmaceutical and food sectors. By converting quinoa husks into valuable bioactive extracts,we contribute significantly to developing effective natural compounds, underlining the significance of our collective work.
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funding.funder.identifier_str_mv http://doi.org/10.13039/501100001871
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funding.funder.name_str_mv Fundação para a Ciência e a Tecnologia
Fundação para a Ciência e a Tecnologia
Fundação para a Ciência e a Tecnologia
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6817 - DCRRNI ID
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identifier.url.fl_str_mv http://hdl.handle.net/10198/30392
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network_name_str Biblioteca Digital do IPB
oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/30392
organization_str_mv urn:organizationAcronym:ipb
person_str_mv Casalvara, Rhaira Fernanda Ayoub
Santos, Everton da Silva
Mattos, Jose Vinicius
Pimentel, Tatiana Colombo
Calhelha, Ricardo C.
Calhelha, Ricardo C.
https://www.ciencia-id.pt/F313-E3CE-554E
F313-E3CE-554E
http://orcid.org/0000-0002-6801-4578
0000-0002-6801-4578
Pires, Tânia C.S.P.
Pires, Tânia C.S.P.
https://www.ciencia-id.pt/321E-2D96-00CA
321E-2D96-00CA
http://orcid.org/0000-0002-3954-3833
0000-0002-3954-3833
Rodrigues, Daniele Bobrowski
Rodrigues, Daniele Bobrowski
https://www.ciencia-id.pt/A91B-FE1D-B275
A91B-FE1D-B275
http://orcid.org/0000-0003-0260-5751
0000-0003-0260-5751
Pereira, Carla
Pereira, Carla
https://www.ciencia-id.pt/EF10-2739-2B70
EF10-2739-2B70
http://orcid.org/0000-0003-0093-771X
0000-0003-0093-771X
Corrêa, Rúbia C.G.
Cardozo‐Filho, Lucio
Gonçalves, José Eduardo
publishDate 2024
publisher.none.fl_str_mv Wiley
reponame_str Biblioteca Digital do IPB
repository_id_str urn:repositoryAcronym:ipb
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spelling engWileypt_PTThis study aimed to investigate the effect of extraction methods and solvents (Soxhlet and supercritical fluid extraction, SFE) on the chemical profile (gas chromatography [GC]/mass spectrometry and GC/flame ionization detection) and antimicrobial, antioxidant, antitumor, and anti-inflammatory activities of quinoa Piabiru (QP) husk crude extracts. Soxhlet was applied using 100% water (QPSH2O), and ethanolic solutions of 50% ethanol (ethanol:water 50:50 v/v, QPSetOH50), 70% ethanol (QPSetOH70), and 99% ethanol (QPSetOH99) as solvents. SFE was applied using CO2 and n-propane as solvents (QPSF). QPSH2O extract showed a higher concentration of phytosterols (stigmasterol, β-sitosterol, 7,8-epoxylanostan-11-OL,3-acetoxy) and carotenoids (rhodopin) and oleic acid, displaying intermediate thiobarbituric acid reactive substance (TBARS) antioxidant activity. Intermediate concentrations of ethanol (QPSetOH50 and QPSetOH70 extracts) increased the extraction yields and the antibacterial activity of the extracts (Pseudomonas aeruginosa, Salmonella enterica, Bacillus cereus, and Staphylococcus aureus). Higher concentrations of ethanol (QPSetOH99 extract) contributed to increased antioxidant activity as assessed by TBARS and higher recoveries of 4-(allyloxy)-2-methyl-2-pentanol, nonadecane, and lauric, myristic, palmitic, linoleic, stearic, arachidic, behenic, and lignoceric acids. Finally, the QPSF extract presented higher antioxidant activity by DPPH, ABTS, and ferric-reducing antioxidant power, higher content of 5-methoxy-2-pentanone, 5-methoxy-2-methyl- 2-pentanol, 1-(1,3-dimethylbutoxy)-2-propanol, oxalic, undecanoic, myristoleic, tricosanoic, pentadecanoic, elaidic, 11-eicosenoic, and erucic acids, and better antifungal activity against Aspergillus brasiliensis than the other extracts. Crude extracts were not cytotoxic against non-tumor cells (Vero) and did not show antitumor or antiinflammatory activities. Thus, antagonistic or synergistic effects of the phytochemical profile of quinoa husk crude extracts may present potential food and pharmaceutical applications. Practical Applications: The exceptional nutritional properties of quinoa seeds have boosted their cultivation in more than 123 countries. However, quinoa husks are generally considered waste. This study, which is of utmost importance, demonstrates the potential of extracting bioactive compounds from quinoa husks via pressurized fluids, turning them into a health-promoting co-product. This approach could minimize the current shortage of new antibiotics, antifungals, antitumor agents, and anti-inflammatory substances in the pharmaceutical and food sectors. By converting quinoa husks into valuable bioactive extracts,we contribute significantly to developing effective natural compounds, underlining the significance of our collective work.application/pdfpt_PTChemical profile and bioactivities of industrial wastes from Chenopodium quinoa seedCasalvara, Rhaira Fernanda AyoubSantos, Everton da SilvaMattos, Jose ViniciusPimentel, Tatiana ColomboPersonalCalhelha, Ricardo C.DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053DSpacehttp://dspace.org/items/2d5d1a41-7561-4a01-871c-b4c97da35053CalhelhaRicardo C.Ciência IDhttps://www.ciencia-id.ptF313-E3CE-554EORCIDhttp://orcid.org0000-0002-6801-4578Researcher IDhttps://www.researcherid.comJ-2172-2014Scopus Author IDhttps://www.scopus.com6507978333PersonalPires, Tânia C.S.P.DSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fDSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fPiresTânia C.S.P.Ciência IDhttps://www.ciencia-id.pt321E-2D96-00CAORCIDhttp://orcid.org0000-0002-3954-3833Researcher IDhttps://www.researcherid.comQ-2842-2018Scopus Author IDhttps://www.scopus.com57057440000PersonalRodrigues, Daniele BobrowskiDSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3DSpacehttp://dspace.org/items/18a661e9-0f77-4bd1-b2b0-5b929b760ab3RodriguesDaniele BobrowskiCiência IDhttps://www.ciencia-id.ptA91B-FE1D-B275ORCIDhttp://orcid.org0000-0003-0260-5751PersonalPereira, CarlaDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bPereiraCarlaCiência IDhttps://www.ciencia-id.ptEF10-2739-2B70ORCIDhttp://orcid.org0000-0003-0093-771XResearcher IDhttps://www.researcherid.comK-1629-2016Scopus Author IDhttps://www.scopus.com55627876424Corrêa, Rúbia C.G.Cardozo‐Filho, LucioGonçalves, José EduardoHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1438-7697ISSNIsPartOf1438-9312DOIIsPartOf10.1002/ejlt.2024000212024-10-09T09:06:45Z20242024-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/30392http://purl.org/coar/access_right/c_abf2open accessExtraction methodsGreen chemistryIndustrial biowastePhytochemicalsQuinoa brasileira2566721 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaAssociate Laboratory for Sustainability and Tecnology in Mountain Regions6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal article2024http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/567ff4be-6dbe-4ae7-a953-3576f6a94094/downloadEuropean Journal of Lipid Science and Technology12610114
spellingShingle Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
Casalvara, Rhaira Fernanda Ayoub
Extraction methods
Green chemistry
Industrial biowaste
Phytochemicals
Quinoa brasileira
status SINGLETON
subject.fl_str_mv Extraction methods
Green chemistry
Industrial biowaste
Phytochemicals
Quinoa brasileira
title Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
title_full Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
title_fullStr Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
title_full_unstemmed Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
title_short Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
title_sort Chemical profile and bioactivities of industrial wastes from Chenopodium quinoa seed
topic Extraction methods
Green chemistry
Industrial biowaste
Phytochemicals
Quinoa brasileira
topic_facet Extraction methods
Green chemistry
Industrial biowaste
Phytochemicals
Quinoa brasileira
url http://hdl.handle.net/10198/30392
visible 1