Publicação
A simple microfluidic device for the deformability assessment of blood cells in a continuous flow
| Resumo: | Blood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability. |
|---|---|
| Autores principais: | Rodrigues, Raquel Oliveira |
| Outros Autores: | Pinho, Diana; Faustino, Vera; Lima, Rui A. |
| Assunto: | Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| Ano: | 2015 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863851433125740544 |
|---|---|
| author | Rodrigues, Raquel Oliveira |
| author2 | Pinho, Diana Faustino, Vera Lima, Rui A. |
| author2_role | author author author |
| author_facet | Rodrigues, Raquel Oliveira Pinho, Diana Faustino, Vera Lima, Rui A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Rodrigues, Raquel Oliveira\"},{\"Person.name\":\"Pinho, Diana\",\"Person.identifier.orcid\":\"0000-0002-3884-6496\"},{\"Person.name\":\"Faustino, Vera\"},{\"Person.name\":\"Lima, Rui A.\",\"Person.identifier.orcid\":\"0000-0003-3428-637X\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Rodrigues, Raquel Oliveira Pinho, Diana Faustino, Vera Lima, Rui A. |
| datacite.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| datacite.titles.title.fl_str_mv | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Rodrigues, Raquel Oliveira Pinho, Diana Faustino, Vera Lima, Rui A. |
| dc.date.Accepted.fl_str_mv | 2015-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/16100 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| dc.title.fl_str_mv | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Blood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/d7f76c4d-755c-446c-b2bb-bea6bb668162/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 5876-PPCDTI FARH |
| id | ipb_e34f2e22f855bc41fedb3edfcb071312 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/16100 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/16100 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Rodrigues, Raquel Oliveira Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Faustino, Vera Lima, Rui A. Lima, Rui A. https://www.ciencia-id.pt/EE12-C3FB-349D EE12-C3FB-349D http://orcid.org/0000-0003-3428-637X 0000-0003-3428-637X |
| publishDate | 2015 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engen_ENBlood flow presents several interesting phenomena in microcirculation that can be used to develop microfluidic devices capable to promote blood cells separation and analysis in continuous flow. In the last decade there have been numerous microfluidic studies focused on the deformation of red blood cells (RBCs) flowing through geometries mimicking microvessels. In contrast, studies focusing on the deformation of white blood cells (WBCs) are scarce despite this phenomenon often happens in the microcirculation. In this work, we present a novel integrative microfluidic device able to perform continuous separation of a desired amount of blood cells, without clogging or jamming, and at the same time, capable to assess the deformation index (DI) of both WBCs and RBCs. To determine the DI of both WBCs and RBCs, a hyperbolic converging microchannel was used, as well as a suitable image analysis technique to measure the DIs of these blood cells along the regions of interest. The results show that the WBCs have a much lower deformability than RBCs when subjected to the same in vitro flow conditions, which is directly related to their cytoskeleton and nucleus contents. The proposed strategy can be easily transformed into a simple and inexpensive diagnostic microfluidic system to simultaneously separate and assess blood cells deformability.application/pdfen_ENA simple microfluidic device for the deformability assessment of blood cells in a continuous flowRodrigues, Raquel OliveiraPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496Faustino, VeraPersonalLima, Rui A.DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62DSpacehttp://dspace.org/items/7b50c499-8095-4f4f-8b1b-fa7388e4ff62LimaRui A.Ciência IDhttps://www.ciencia-id.ptEE12-C3FB-349DORCIDhttp://orcid.org0000-0003-3428-637XResearcher IDhttps://www.researcherid.comH-5157-2016Scopus Author IDhttps://www.scopus.com18437397800HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1387-2176DOIIsPartOf10.1007/s10544-015-0014-22018-01-19T10:00:00Z20152015-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/16100http://purl.org/coar/access_right/c_abf2open accessBlood on chipsCell separation and deformabilityHyperbolic microchannelMicrofluidic devicesRBCWBC1147248 bytesFundação para a Ciência e a TecnologiaBIOMIMETIC - A biomimetic microdevice for the diagnosis of erythrocytes diseases based on their deformability5876-PPCDTICrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMAGNETIC CARBON NANOSTRUCTURES AND STUDY OF THEIR TRANSPORT IN MICROFLUIDIC DEVICES FOR HYPERTHERMIACrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMicrofluidic system for cell separation and deformation assessment based on biomimetic and acoustofluidic methodsFARHCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/d7f76c4d-755c-446c-b2bb-bea6bb668162/download |
| spellingShingle | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow Rodrigues, Raquel Oliveira Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| subject.fl_str_mv | Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| title | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| title_full | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| title_fullStr | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| title_full_unstemmed | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| title_short | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| title_sort | A simple microfluidic device for the deformability assessment of blood cells in a continuous flow |
| topic | Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| topic_facet | Blood on chips Cell separation and deformability Hyperbolic microchannel Microfluidic devices RBC WBC |
| url | http://hdl.handle.net/10198/16100 |
| visible | 1 |