Publicação
Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives
| Resumo: | Consumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including Calendula officinalis L., Calluna vulgaris (L.) Hull, Hippophae rhamnoides L., Juglans regia L., Mentha cervina L., Rubus idaeus L., Sambucus nigra L., and Vitis vinifera L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 & PLUSMN; 0.2 (V. vinifera) to 123 & PLUSMN; 2 mg/g (H. rhamnoides) of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. S. nigra showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and H. rhamnoides, notably, had the lowest IC50 values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples C. vulgaris, V. vinifera and R. idaeus might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using Artemia franciscana suggest low toxicity for most extracts, with LC50 > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain. |
|---|---|
| Autores principais: | Deus, Breno Martins de |
| Outros Autores: | Fernandes, Conceição; Molina, Adriana K.; Xavier, Virginie; Pires, Tânia C.S.P.; Mandim, Filipa; Heleno, Sandrina A.; Finimundy, Tiane C.; Barros, Lillian |
| Assunto: | Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| Ano: | 2023 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172852598308864 |
|---|---|
| author | Deus, Breno Martins de |
| author2 | Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
| author2_role | author author author author author author author author |
| author_facet | Deus, Breno Martins de Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Deus, Breno Martins de\"},{\"Person.name\":\"Fernandes, Conceição\",\"Person.identifier.orcid\":\"0000-0003-2873-501X\"},{\"Person.name\":\"Molina, Adriana K.\",\"Person.identifier.orcid\":\"0000-0002-6234-2212\"},{\"Person.name\":\"Xavier, Virginie\",\"Person.identifier.orcid\":\"0000-0001-7508-1245\"},{\"Person.name\":\"Pires, Tânia C.S.P.\",\"Person.identifier.orcid\":\"0000-0002-3954-3833\"},{\"Person.name\":\"Mandim, Filipa\",\"Person.identifier.orcid\":\"0000-0002-6494-2326\"},{\"Person.name\":\"Heleno, Sandrina A.\",\"Person.identifier.orcid\":\"0000-0001-7224-1098\"},{\"Person.name\":\"Finimundy, Tiane C.\",\"Person.identifier.orcid\":\"0000-0003-3516-0795\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Deus, Breno Martins de Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
| datacite.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-05-10T09:16:39Z |
| datacite.date.embargoed.fl_str_mv | 2023-05-10T09:16:39Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| datacite.titles.title.fl_str_mv | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Deus, Breno Martins de Fernandes, Conceição Molina, Adriana K. Xavier, Virginie Pires, Tânia C.S.P. Mandim, Filipa Heleno, Sandrina A. Finimundy, Tiane C. Barros, Lillian |
| dc.date.Accepted.fl_str_mv | 2023-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-05-10T09:16:39Z |
| dc.date.embargoed.fl_str_mv | 2023-05-10T09:16:39Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/28263 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| dc.title.fl_str_mv | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Consumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including Calendula officinalis L., Calluna vulgaris (L.) Hull, Hippophae rhamnoides L., Juglans regia L., Mentha cervina L., Rubus idaeus L., Sambucus nigra L., and Vitis vinifera L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 & PLUSMN; 0.2 (V. vinifera) to 123 & PLUSMN; 2 mg/g (H. rhamnoides) of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. S. nigra showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and H. rhamnoides, notably, had the lowest IC50 values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples C. vulgaris, V. vinifera and R. idaeus might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using Artemia franciscana suggest low toxicity for most extracts, with LC50 > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/20394d1d-36ea-4e73-b3fe-14ec6691f431/download |
| id | ipb_e36f6efb4129b9c19a98aa3ff8a67d1e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/28263 |
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| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
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| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Deus, Breno Martins de Fernandes, Conceição Fernandes, Conceição https://www.ciencia-id.pt/6514-3D41-4534 6514-3D41-4534 http://orcid.org/0000-0003-2873-501X 0000-0003-2873-501X Molina, Adriana K. Molina, Adriana K. https://www.ciencia-id.pt/C110-0167-E58D C110-0167-E58D http://orcid.org/0000-0002-6234-2212 0000-0002-6234-2212 Xavier, Virginie Xavier, Virginie https://www.ciencia-id.pt/B212-37BC-7FB4 B212-37BC-7FB4 http://orcid.org/0000-0001-7508-1245 0000-0001-7508-1245 Pires, Tânia C.S.P. Pires, Tânia C.S.P. https://www.ciencia-id.pt/321E-2D96-00CA 321E-2D96-00CA http://orcid.org/0000-0002-3954-3833 0000-0002-3954-3833 Mandim, Filipa Mandim, Filipa https://www.ciencia-id.pt/F318-9049-EB99 F318-9049-EB99 http://orcid.org/0000-0002-6494-2326 0000-0002-6494-2326 Heleno, Sandrina A. Heleno, Sandrina A. https://www.ciencia-id.pt/8B18-3095-6FC9 8B18-3095-6FC9 http://orcid.org/0000-0001-7224-1098 0000-0001-7224-1098 Finimundy, Tiane C. Finimundy, Tiane C. https://www.ciencia-id.pt/2F18-67B1-776A 2F18-67B1-776A http://orcid.org/0000-0003-3516-0795 0000-0003-3516-0795 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 |
| publishDate | 2023 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engMDPIpt_PTConsumer demand for natural and healthier products has led to an increasing interest in the bioactive and therapeutic properties of plant extracts. In this study, we evaluated the phenolic compounds profile, bioactivities, and toxicities of plant extracts from eight European flora species, including Calendula officinalis L., Calluna vulgaris (L.) Hull, Hippophae rhamnoides L., Juglans regia L., Mentha cervina L., Rubus idaeus L., Sambucus nigra L., and Vitis vinifera L. The aim was to identify potential preservatives of natural origin. Phenolic compounds were identified by HPLC-DAD-ESI-MS. Caffeic acid derivatives, ellagitannins, flavonols, and flavones were the major phenolic compounds identified. The total phenolic content varied from 16.0 & PLUSMN; 0.2 (V. vinifera) to 123 & PLUSMN; 2 mg/g (H. rhamnoides) of dry extract. All extracts showed antioxidant potential and exhibited activity against some of the microorganisms tested. S. nigra showed the highest activity in the inhibition of oxidative hemolysis (OxHLIA) assay and H. rhamnoides, notably, had the lowest IC50 values in TBARS and DPPH assays, as well as the lowest minimum inhibitory concentration (MIC) values. Regarding in vitro cytotoxicity, in tumor and non-tumor cell lines, although some extracts revealed toxicity against normal cells, it was found that the samples C. vulgaris, V. vinifera and R. idaeus might be used against tumor cells since the active concentration is much lower than the one causing toxicity. In vivo acute toxicity tests using Artemia franciscana suggest low toxicity for most extracts, with LC50 > 400 mg/L. These results showed the potential of the studied extracts as natural preservatives, given their richness in compounds with bioactive properties, highlight their potential value to the production chain.application/pdfpt_PTChemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservativesDeus, Breno Martins dePersonalFernandes, ConceiçãoDSpacehttp://dspace.org/items/d9c26c54-5e10-4ccb-b7f7-528af9eb9950DSpacehttp://dspace.org/items/d9c26c54-5e10-4ccb-b7f7-528af9eb9950FernandesConceiçãoCiência IDhttps://www.ciencia-id.pt6514-3D41-4534ORCIDhttp://orcid.org0000-0003-2873-501XScopus Author IDhttps://www.scopus.com23018336600PersonalMolina, Adriana K.DSpacehttp://dspace.org/items/dc9b5b67-c45e-4fbe-8af1-8cec9adb5cd9DSpacehttp://dspace.org/items/dc9b5b67-c45e-4fbe-8af1-8cec9adb5cd9MolinaAdriana K.Ciência IDhttps://www.ciencia-id.ptC110-0167-E58DORCIDhttp://orcid.org0000-0002-6234-2212PersonalXavier, VirginieDSpacehttp://dspace.org/items/e11787fd-f254-4635-a4c3-c56e829f614eDSpacehttp://dspace.org/items/e11787fd-f254-4635-a4c3-c56e829f614eXavierVirginieCiência IDhttps://www.ciencia-id.ptB212-37BC-7FB4ORCIDhttp://orcid.org0000-0001-7508-1245PersonalPires, Tânia C.S.P.DSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fDSpacehttp://dspace.org/items/34af4d18-b8ae-4329-9450-69dc35ed448fPiresTânia C.S.P.Ciência IDhttps://www.ciencia-id.pt321E-2D96-00CAORCIDhttp://orcid.org0000-0002-3954-3833Researcher IDhttps://www.researcherid.comQ-2842-2018Scopus Author IDhttps://www.scopus.com57057440000PersonalMandim, FilipaDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdMandimFilipaCiência IDhttps://www.ciencia-id.ptF318-9049-EB99ORCIDhttp://orcid.org0000-0002-6494-2326PersonalHeleno, Sandrina A.DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676DSpacehttp://dspace.org/items/e89b5bf5-d315-49b6-871e-fa8eb6030676HelenoSandrina A.Ciência IDhttps://www.ciencia-id.pt8B18-3095-6FC9ORCIDhttp://orcid.org0000-0001-7224-1098Researcher IDhttps://www.researcherid.comL-5951-2014Scopus Author IDhttps://www.scopus.com30067731800PersonalFinimundy, Tiane C.DSpacehttp://dspace.org/items/4f8767d4-4e62-4550-9b35-740cb2f672daDSpacehttp://dspace.org/items/4f8767d4-4e62-4550-9b35-740cb2f672daFinimundyTiane C.Ciência IDhttps://www.ciencia-id.pt2F18-67B1-776AORCIDhttp://orcid.org0000-0003-3516-0795PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.3390/plants121527842023-05-10T09:16:39Z20232023-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/28263http://purl.org/coar/access_right/c_abf2open accessPhenolic compoundsPlant extractsNatural preservativesBioactivityToxicityArtemia franciscanaEuropean flora560625 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal article2023http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/20394d1d-36ea-4e73-b3fe-14ec6691f431/downloadPlants |
| spellingShingle | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives Deus, Breno Martins de Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| status | SINGLETON |
| subject.fl_str_mv | Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| title | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| title_full | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| title_fullStr | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| title_full_unstemmed | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| title_short | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| title_sort | Chemical characterization, bioactivity and toxicity of european flora plant extracts in search for potential natural origin preservatives |
| topic | Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| topic_facet | Phenolic compounds Plant extracts Natural preservatives Bioactivity Toxicity Artemia franciscana European flora |
| url | http://hdl.handle.net/10198/28263 |
| visible | 1 |