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Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development

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Resumo:The use of bio-residues from fruits and vegetables such as seeds, peels, aerial parts, etc., in the food industry has been of great interest due to the possibility of developing new secondary added-value materials such as natural colorants, which not only provide a colour to the food or product, but also provide health benefits to the consumer (Tiwari et al. 2019). In the present study, the extraction of chlorophylls from the aerial parts of carrots was carried out in order to develop natural food colourants. For that purpose, two types of extraction (maceration and ultrasound-assisted extraction) were performed to maximise the yield of chlorophyll recovery. Three types of solvents were used, prioritising green solvents (water, 90% ethanol, and hexane). At the same time, different parameters were varied in each of the techniques: i) in maceration: extraction time and solvent; ii) in ultrasound-assisted extraction: power and solvent. The extractions were carried out protecting the samples from light and the results were obtained using a newly developed chromatographic method through high performance liquid chromatography (HPLC) coupled to a diode array detector (DAD) and mass spectrometry (MS). In general, the aerial parts of carrot revealed as main compounds chlorophylls a and b, as well as derivatives in significant concentrations. The ultrasound technique proved to be more efficient than maceration extraction, with higher extraction yields when higher ultrasonic power was used. Compared to water and hexane, ethanol allowed the extraction of greater amounts of chlorophylls. These compounds can find application in food industry, but also in other industrial fields, given their high coloring properties, making these bioresidues valuable sources to exploit for colorants development.
Autores principais:Molina, Adriana K.
Outros Autores:Gomes, Leonardo Corrêa; Palmeira, Luís; Pereira, Carla; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Barros, Lillian
Assunto:Chlorophylls Daucus Carota L.
Ano:2021
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Molina, Adriana K.
author2 Gomes, Leonardo Corrêa
Palmeira, Luís
Pereira, Carla
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Barros, Lillian
author2_role author
author
author
author
author
author
author_facet Molina, Adriana K.
Gomes, Leonardo Corrêa
Palmeira, Luís
Pereira, Carla
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Barros, Lillian
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Molina, Adriana K.\",\"Person.identifier.orcid\":\"0000-0002-6234-2212\"},{\"Person.name\":\"Gomes, Leonardo Corrêa\"},{\"Person.name\":\"Palmeira, Luís\",\"Person.identifier.orcid\":\"0000-0003-3698-5641\"},{\"Person.name\":\"Pereira, Carla\",\"Person.identifier.orcid\":\"0000-0003-0093-771X\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Molina, Adriana K.
Gomes, Leonardo Corrêa
Palmeira, Luís
Pereira, Carla
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Barros, Lillian
datacite.date.Accepted.fl_str_mv 2021-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2022-01-24T12:02:04Z
datacite.date.embargoed.fl_str_mv 2022-01-24T12:02:04Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Chlorophylls
Daucus Carota L.
datacite.titles.title.fl_str_mv Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Molina, Adriana K.
Gomes, Leonardo Corrêa
Palmeira, Luís
Pereira, Carla
Dias, Maria Inês
Ferreira, Isabel C.F.R.
Barros, Lillian
dc.date.Accepted.fl_str_mv 2021-01-01T00:00:00Z
dc.date.available.fl_str_mv 2022-01-24T12:02:04Z
dc.date.embargoed.fl_str_mv 2022-01-24T12:02:04Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/24875
dc.language.none.fl_str_mv eng
dc.rights.cclincense.fl_str_mv http://creativecommons.org/licenses/by/4.0/
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Chlorophylls
Daucus Carota L.
dc.title.fl_str_mv Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description The use of bio-residues from fruits and vegetables such as seeds, peels, aerial parts, etc., in the food industry has been of great interest due to the possibility of developing new secondary added-value materials such as natural colorants, which not only provide a colour to the food or product, but also provide health benefits to the consumer (Tiwari et al. 2019). In the present study, the extraction of chlorophylls from the aerial parts of carrots was carried out in order to develop natural food colourants. For that purpose, two types of extraction (maceration and ultrasound-assisted extraction) were performed to maximise the yield of chlorophyll recovery. Three types of solvents were used, prioritising green solvents (water, 90% ethanol, and hexane). At the same time, different parameters were varied in each of the techniques: i) in maceration: extraction time and solvent; ii) in ultrasound-assisted extraction: power and solvent. The extractions were carried out protecting the samples from light and the results were obtained using a newly developed chromatographic method through high performance liquid chromatography (HPLC) coupled to a diode array detector (DAD) and mass spectrometry (MS). In general, the aerial parts of carrot revealed as main compounds chlorophylls a and b, as well as derivatives in significant concentrations. The ultrasound technique proved to be more efficient than maceration extraction, with higher extraction yields when higher ultrasonic power was used. Compared to water and hexane, ethanol allowed the extraction of greater amounts of chlorophylls. These compounds can find application in food industry, but also in other industrial fields, given their high coloring properties, making these bioresidues valuable sources to exploit for colorants development.
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person_str_mv Molina, Adriana K.
Molina, Adriana K.
https://www.ciencia-id.pt/C110-0167-E58D
C110-0167-E58D
http://orcid.org/0000-0002-6234-2212
0000-0002-6234-2212
Gomes, Leonardo Corrêa
Palmeira, Luís
Palmeira, Luís
https://www.ciencia-id.pt/FE12-0309-F7C9
FE12-0309-F7C9
http://orcid.org/0000-0003-3698-5641
0000-0003-3698-5641
Pereira, Carla
Pereira, Carla
https://www.ciencia-id.pt/EF10-2739-2B70
EF10-2739-2B70
http://orcid.org/0000-0003-0093-771X
0000-0003-0093-771X
Dias, Maria Inês
Dias, Maria Inês
https://www.ciencia-id.pt/2A13-4BE6-C7CF
2A13-4BE6-C7CF
http://orcid.org/0000-0001-8744-7814
0000-0001-8744-7814
Ferreira, Isabel C.F.R.
Ferreira, Isabel C.F.R.
https://www.ciencia-id.pt/9418-CF95-9919
9418-CF95-9919
http://orcid.org/0000-0003-4910-4882
0000-0003-4910-4882
Barros, Lillian
Barros, Lillian
https://www.ciencia-id.pt/9616-35CB-D001
9616-35CB-D001
http://orcid.org/0000-0002-9050-5189
0000-0002-9050-5189
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spelling engpt_PTThe use of bio-residues from fruits and vegetables such as seeds, peels, aerial parts, etc., in the food industry has been of great interest due to the possibility of developing new secondary added-value materials such as natural colorants, which not only provide a colour to the food or product, but also provide health benefits to the consumer (Tiwari et al. 2019). In the present study, the extraction of chlorophylls from the aerial parts of carrots was carried out in order to develop natural food colourants. For that purpose, two types of extraction (maceration and ultrasound-assisted extraction) were performed to maximise the yield of chlorophyll recovery. Three types of solvents were used, prioritising green solvents (water, 90% ethanol, and hexane). At the same time, different parameters were varied in each of the techniques: i) in maceration: extraction time and solvent; ii) in ultrasound-assisted extraction: power and solvent. The extractions were carried out protecting the samples from light and the results were obtained using a newly developed chromatographic method through high performance liquid chromatography (HPLC) coupled to a diode array detector (DAD) and mass spectrometry (MS). In general, the aerial parts of carrot revealed as main compounds chlorophylls a and b, as well as derivatives in significant concentrations. The ultrasound technique proved to be more efficient than maceration extraction, with higher extraction yields when higher ultrasonic power was used. Compared to water and hexane, ethanol allowed the extraction of greater amounts of chlorophylls. These compounds can find application in food industry, but also in other industrial fields, given their high coloring properties, making these bioresidues valuable sources to exploit for colorants development.application/pdfpt_PTExtraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants developmentPersonalMolina, Adriana K.DSpacehttp://dspace.org/items/dc9b5b67-c45e-4fbe-8af1-8cec9adb5cd9DSpacehttp://dspace.org/items/dc9b5b67-c45e-4fbe-8af1-8cec9adb5cd9MolinaAdriana K.Ciência IDhttps://www.ciencia-id.ptC110-0167-E58DORCIDhttp://orcid.org0000-0002-6234-2212Gomes, Leonardo CorrêaPersonalPalmeira, LuísDSpacehttp://dspace.org/items/84c7fd05-f69f-43ef-8449-eabcdfa9c1f6DSpacehttp://dspace.org/items/84c7fd05-f69f-43ef-8449-eabcdfa9c1f6PalmeiraLuísCiência IDhttps://www.ciencia-id.ptFE12-0309-F7C9ORCIDhttp://orcid.org0000-0003-3698-5641PersonalPereira, CarlaDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bDSpacehttp://dspace.org/items/e07a8e12-52bb-47c5-bae7-41a5f648213bPereiraCarlaCiência IDhttps://www.ciencia-id.ptEF10-2739-2B70ORCIDhttp://orcid.org0000-0003-0093-771XResearcher IDhttps://www.researcherid.comK-1629-2016Scopus Author IDhttps://www.scopus.com55627876424PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000PersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISBNIsPartOf978-618-84774-2-1DOIIsPartOfMolina, Adriana K.; Gomes, Leonardo C.; Palmeira, Luis; Pereira, Carla; Dias, Maria Inês; Ferreira, Isabel C.F.R.; Barros, Lillian (2021). Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development. In RETASTE: Rethink Food Waste. Atenas, Grécia. ISBN 978-618-84774-2-12022-01-24T12:02:04Z20212021-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/24875http://purl.org/coar/access_right/c_abf2open accessChlorophyllsDaucus Carota L.2990597 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2021http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/e98fa1aa-5f3c-4797-a4a5-250285c7277f/downloadRETASTE: Rethink Food WasteAtenas, Grécia
spellingShingle Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
Molina, Adriana K.
Chlorophylls
Daucus Carota L.
status SINGLETON
subject.fl_str_mv Chlorophylls
Daucus Carota L.
title Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
title_full Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
title_fullStr Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
title_full_unstemmed Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
title_short Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
title_sort Extraction of Chlorophylls from bioresidues of Daucus Carota L. (Carrots) aerial parts for food colorants development
topic Chlorophylls
Daucus Carota L.
topic_facet Chlorophylls
Daucus Carota L.
url http://hdl.handle.net/10198/24875
visible 1