Publicação
Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii
| Resumo: | Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial “O” lineage, rather than a diagnostic site for A. m. lamarckii. |
|---|---|
| Autores principais: | Kandemir, Irfan |
| Outros Autores: | Pinto, M. Alice; Meixner, Marina D.; Sheppard, Walter S. |
| Assunto: | mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| Ano: | 2006 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172777883074560 |
|---|---|
| author | Kandemir, Irfan |
| author2 | Pinto, M. Alice Meixner, Marina D. Sheppard, Walter S. |
| author2_role | author author author |
| author_facet | Kandemir, Irfan Pinto, M. Alice Meixner, Marina D. Sheppard, Walter S. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Kandemir, Irfan\"},{\"Person.name\":\"Pinto, M. Alice\",\"Person.identifier.orcid\":\"0000-0001-9663-8399\"},{\"Person.name\":\"Meixner, Marina D.\"},{\"Person.name\":\"Sheppard, Walter S.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Kandemir, Irfan Pinto, M. Alice Meixner, Marina D. Sheppard, Walter S. |
| datacite.date.Accepted.fl_str_mv | 2006-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2010-12-03T12:09:12Z |
| datacite.date.embargoed.fl_str_mv | 2010-12-03T12:09:12Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| datacite.titles.title.fl_str_mv | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Kandemir, Irfan Pinto, M. Alice Meixner, Marina D. Sheppard, Walter S. |
| dc.date.Accepted.fl_str_mv | 2006-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2010-12-03T12:09:12Z |
| dc.date.embargoed.fl_str_mv | 2010-12-03T12:09:12Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/2892 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Genetics and Molecular Biology |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| dc.title.fl_str_mv | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial “O” lineage, rather than a diagnostic site for A. m. lamarckii. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/b5b4ae06-941f-4bbb-9b81-0ddd0594bc8e/download |
| id | ipb_e7e255e90002b6ef2a1fdeaded03516d |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/2892 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/2892 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Kandemir, Irfan Pinto, M. Alice Pinto, M. Alice https://www.ciencia-id.pt/F814-A1D0-8318 F814-A1D0-8318 http://orcid.org/0000-0001-9663-8399 0000-0001-9663-8399 Meixner, Marina D. Sheppard, Walter S. |
| publishDate | 2006 |
| publisher.none.fl_str_mv | Genetics and Molecular Biology |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engGenetics and Molecular BiologyporRestriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial “O” lineage, rather than a diagnostic site for A. m. lamarckii.application/pdfporHinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckiiKandemir, IrfanPersonalPinto, M. AliceDSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5DSpacehttp://dspace.org/items/0667fe04-7078-483d-9198-56d167b19bc5PintoM. AliceCiência IDhttps://www.ciencia-id.ptF814-A1D0-8318ORCIDhttp://orcid.org0000-0001-9663-8399Scopus Author IDhttps://www.scopus.com8085507800Meixner, Marina D.Sheppard, Walter S.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1415-4757DOIIsPartOf10.1590/S1415-475720060004000272010-12-03T12:09:12Z20062006-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/2892http://purl.org/coar/access_right/c_abf2open accessmtDNACytochrome oxidase IApis mellifera lamarckiiO lineage54541 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/b5b4ae06-941f-4bbb-9b81-0ddd0594bc8e/downloadGenetics and Molecular Biology747749 |
| spellingShingle | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii Kandemir, Irfan mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| status | SINGLETON |
| subject.fl_str_mv | mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| title | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| title_full | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| title_fullStr | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| title_full_unstemmed | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| title_short | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| title_sort | Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A-m. lamarckii |
| topic | mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| topic_facet | mtDNA Cytochrome oxidase I Apis mellifera lamarckii O lineage |
| url | http://hdl.handle.net/10198/2892 |
| visible | 1 |