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Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results

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Resumo:Due to the problems caused by the use of chemical insecticides for humans and environment alternative pest control methods are an important topic of research. The use of microbial insecticides especially fungal agents are an attractive and promising alternative for biological control of insect pests. The aim of this work was to identify naturally occurring entomophatogenic fungi on the olive moth, Prays oleae Bern., in the northeast of Portugal, as first step to select biological control agent again this olive pest. The experimental work was carried out during 2007 in the three generation of the insect (phyllophagous, anthophagous and carpophagous generation). In each generation P. oleae larvae and pupae were collected in different groves and were put in glass vials in a climatic chamber with a photoperiod of 12h light:12h dark, 22ºC (light): 16ºC (dark) and 60% relative humidity, until emergency of the adults. From dead larvae, fungi were isolated on PDA plates and incubated at room temperature. Pure cultures were morphological and molecularly identified based on the ITS region of the rDNA. From the identified species Beauveria bassiana Vuill. and Cordyceps sinensis (Berk.) Sacc. were the most promising being B. bassiana the most abundant one.
Autores principais:Baptista, Paula
Outros Autores:Pereira, Eric Carvalho; Nogueira, Liliana; Bento, Albino; Santiago-Álvarez, C.; Quesada-Moraga, Enrique; Pereira, J.A.
Assunto:Entomopathogenic fungi Prays oleae Bern. Beauveria bassiana (Bals.) Vuill.
Ano:2008
País:Portugal
Tipo de documento:documento de conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB
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author Baptista, Paula
author2 Pereira, Eric Carvalho
Nogueira, Liliana
Bento, Albino
Santiago-Álvarez, C.
Quesada-Moraga, Enrique
Pereira, J.A.
author2_role author
author
author
author
author
author
author_facet Baptista, Paula
Pereira, Eric Carvalho
Nogueira, Liliana
Bento, Albino
Santiago-Álvarez, C.
Quesada-Moraga, Enrique
Pereira, J.A.
author_role author
contributor_name_str_mv Biblioteca Digital do IPB
country_str PT
creators_json_txt [{\"Person.name\":\"Baptista, Paula\",\"Person.identifier.orcid\":\"0000-0001-6331-3731\"},{\"Person.name\":\"Pereira, Eric Carvalho\"},{\"Person.name\":\"Nogueira, Liliana\"},{\"Person.name\":\"Bento, Albino\",\"Person.identifier.orcid\":\"0000-0001-5215-785X\"},{\"Person.name\":\"Santiago-Álvarez, C.\"},{\"Person.name\":\"Quesada-Moraga, Enrique\"},{\"Person.name\":\"Pereira, J.A.\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\"}]
datacite.contributors.contributor.contributorName.fl_str_mv Biblioteca Digital do IPB
datacite.creators.creator.creatorName.fl_str_mv Baptista, Paula
Pereira, Eric Carvalho
Nogueira, Liliana
Bento, Albino
Santiago-Álvarez, C.
Quesada-Moraga, Enrique
Pereira, J.A.
datacite.date.Accepted.fl_str_mv 2008-01-01T00:00:00Z
datacite.date.available.fl_str_mv 2011-07-05T10:03:30Z
datacite.date.embargoed.fl_str_mv 2011-07-05T10:03:30Z
datacite.rights.fl_str_mv http://purl.org/coar/access_right/c_abf2
datacite.subjects.subject.fl_str_mv Entomopathogenic fungi
Prays oleae Bern.
Beauveria bassiana (Bals.) Vuill.
datacite.titles.title.fl_str_mv Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.creator.none.fl_str_mv Baptista, Paula
Pereira, Eric Carvalho
Nogueira, Liliana
Bento, Albino
Santiago-Álvarez, C.
Quesada-Moraga, Enrique
Pereira, J.A.
dc.date.Accepted.fl_str_mv 2008-01-01T00:00:00Z
dc.date.available.fl_str_mv 2011-07-05T10:03:30Z
dc.date.embargoed.fl_str_mv 2011-07-05T10:03:30Z
dc.format.none.fl_str_mv application/pdf
dc.identifier.none.fl_str_mv http://hdl.handle.net/10198/5739
dc.language.none.fl_str_mv eng
dc.rights.none.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.subject.none.fl_str_mv Entomopathogenic fungi
Prays oleae Bern.
Beauveria bassiana (Bals.) Vuill.
dc.title.fl_str_mv Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
dc.type.none.fl_str_mv http://purl.org/coar/resource_type/c_c94f
description Due to the problems caused by the use of chemical insecticides for humans and environment alternative pest control methods are an important topic of research. The use of microbial insecticides especially fungal agents are an attractive and promising alternative for biological control of insect pests. The aim of this work was to identify naturally occurring entomophatogenic fungi on the olive moth, Prays oleae Bern., in the northeast of Portugal, as first step to select biological control agent again this olive pest. The experimental work was carried out during 2007 in the three generation of the insect (phyllophagous, anthophagous and carpophagous generation). In each generation P. oleae larvae and pupae were collected in different groves and were put in glass vials in a climatic chamber with a photoperiod of 12h light:12h dark, 22ºC (light): 16ºC (dark) and 60% relative humidity, until emergency of the adults. From dead larvae, fungi were isolated on PDA plates and incubated at room temperature. Pure cultures were morphological and molecularly identified based on the ITS region of the rDNA. From the identified species Beauveria bassiana Vuill. and Cordyceps sinensis (Berk.) Sacc. were the most promising being B. bassiana the most abundant one.
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eu_rights_str_mv openAccess
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fulltext.url.fl_str_mv https://bibliotecadigital.ipb.pt/bitstreams/796f691b-7d3e-45b1-8d7f-8901e2a66f33/download
id ipb_ea9348cf01443c4d80d3e77aaa97371f
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institution Instituto Politécnico de Bragança
instname_str Instituto Politécnico de Bragança
language eng
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network_name_str Biblioteca Digital do IPB
oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/5739
organization_str_mv urn:organizationAcronym:ipb
person_str_mv Baptista, Paula
Baptista, Paula
https://www.ciencia-id.pt/7D11-FE1E-CD0F
7D11-FE1E-CD0F
http://orcid.org/0000-0001-6331-3731
0000-0001-6331-3731
Pereira, Eric Carvalho
Nogueira, Liliana
Bento, Albino
Bento, Albino
https://www.ciencia-id.pt/D516-325A-9AD7
D516-325A-9AD7
http://orcid.org/0000-0001-5215-785X
0000-0001-5215-785X
Santiago-Álvarez, C.
Quesada-Moraga, Enrique
Pereira, J.A.
Pereira, J.A.
https://www.ciencia-id.pt/611F-80B2-A7C1
611F-80B2-A7C1
http://orcid.org/0000-0002-2260-0600
0000-0002-2260-0600
publishDate 2008
reponame_str Biblioteca Digital do IPB
repository_id_str urn:repositoryAcronym:ipb
service_str_mv urn:repositoryAcronym:ipb
spelling engporDue to the problems caused by the use of chemical insecticides for humans and environment alternative pest control methods are an important topic of research. The use of microbial insecticides especially fungal agents are an attractive and promising alternative for biological control of insect pests. The aim of this work was to identify naturally occurring entomophatogenic fungi on the olive moth, Prays oleae Bern., in the northeast of Portugal, as first step to select biological control agent again this olive pest. The experimental work was carried out during 2007 in the three generation of the insect (phyllophagous, anthophagous and carpophagous generation). In each generation P. oleae larvae and pupae were collected in different groves and were put in glass vials in a climatic chamber with a photoperiod of 12h light:12h dark, 22ºC (light): 16ºC (dark) and 60% relative humidity, until emergency of the adults. From dead larvae, fungi were isolated on PDA plates and incubated at room temperature. Pure cultures were morphological and molecularly identified based on the ITS region of the rDNA. From the identified species Beauveria bassiana Vuill. and Cordyceps sinensis (Berk.) Sacc. were the most promising being B. bassiana the most abundant one.application/pdfporEntomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary resultsPersonalBaptista, PaulaDSpacehttp://dspace.org/items/3f35226a-b17a-4f7d-8da1-3297105cbfe9DSpacehttp://dspace.org/items/3f35226a-b17a-4f7d-8da1-3297105cbfe9BaptistaPaulaCiência IDhttps://www.ciencia-id.pt7D11-FE1E-CD0FORCIDhttp://orcid.org0000-0001-6331-3731Scopus Author IDhttps://www.scopus.com14051688000Scopus Author IDhttps://www.scopus.com57394839600Pereira, Eric CarvalhoNogueira, LilianaPersonalBento, AlbinoDSpacehttp://dspace.org/items/233115be-9d46-49d0-8b7d-2d64406d64a0DSpacehttp://dspace.org/items/233115be-9d46-49d0-8b7d-2d64406d64a0BentoAlbinoCiência IDhttps://www.ciencia-id.ptD516-325A-9AD7ORCIDhttp://orcid.org0000-0001-5215-785XResearcher IDhttps://www.researcherid.comN-9706-2016Scopus Author IDhttps://www.scopus.com35247694000Santiago-Álvarez, C.Quesada-Moraga, EnriquePersonalPereira, J.A.DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2011-07-05T10:03:30Z20082008-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/5739http://purl.org/coar/access_right/c_abf2open accessEntomopathogenic fungiPrays oleae Bern.Beauveria bassiana (Bals.) Vuill.567432 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/796f691b-7d3e-45b1-8d7f-8901e2a66f33/downloadThe Sixth International Symposium on olive GrowingÉvora, Portugal
spellingShingle Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
Baptista, Paula
Entomopathogenic fungi
Prays oleae Bern.
Beauveria bassiana (Bals.) Vuill.
status SINGLETON
subject.fl_str_mv Entomopathogenic fungi
Prays oleae Bern.
Beauveria bassiana (Bals.) Vuill.
title Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
title_full Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
title_fullStr Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
title_full_unstemmed Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
title_short Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
title_sort Entomopathogenic fungi associated with the main insect pest in the Northeast of Portugal: preliminary results
topic Entomopathogenic fungi
Prays oleae Bern.
Beauveria bassiana (Bals.) Vuill.
topic_facet Entomopathogenic fungi
Prays oleae Bern.
Beauveria bassiana (Bals.) Vuill.
url http://hdl.handle.net/10198/5739
visible 1