Publicação
Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers
| Resumo: | A physiological approach to assessing the antioxidant potential of Arbequina EVOO from different zones of Brazil and Spain was performed, applying a combined model of simulated digestion and cell cultures, using the Caco-2 cell line. Our results showed an increasing of total phenolic content (TPC) and antioxidant properties promoted by the in vitro digestion. Preincubating Caco-2 cells with bioaccessible fractions of oils counteracted the cytotoxic effect promoted by an oxidising agent (t-BOOH), preserving cell viability and reducing the generation of reactive oxygen species (ROS). The protective effect on ROS production was associated with the antioxidant activity (DPPH assay), but no relation with the TPC of the digested samples was found. Differences in the parameters evaluated were observed among the samples, which were related to climatic characteristics of the production zones. It was concluded that transformations during the digestive process are important for establishing the antioxidant potential of the oils. |
|---|---|
| Autores principais: | Borges, Thays H. |
| Outros Autores: | Pereira, J.A.; Cabrera-Vique, Carmen; Seiquer, Isabel |
| Assunto: | Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| Ano: | 2017 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172975146434560 |
|---|---|
| author | Borges, Thays H. |
| author2 | Pereira, J.A. Cabrera-Vique, Carmen Seiquer, Isabel |
| author2_role | author author author |
| author_facet | Borges, Thays H. Pereira, J.A. Cabrera-Vique, Carmen Seiquer, Isabel |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Borges, Thays H.\"},{\"Person.name\":\"Pereira, J.A.\",\"Person.identifier.orcid\":\"0000-0002-2260-0600\"},{\"Person.name\":\"Cabrera-Vique, Carmen\"},{\"Person.name\":\"Seiquer, Isabel\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Borges, Thays H. Pereira, J.A. Cabrera-Vique, Carmen Seiquer, Isabel |
| datacite.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-31T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-31T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| datacite.titles.title.fl_str_mv | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Borges, Thays H. Pereira, J.A. Cabrera-Vique, Carmen Seiquer, Isabel |
| dc.date.Accepted.fl_str_mv | 2017-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-31T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-31T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/15379 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| dc.title.fl_str_mv | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | A physiological approach to assessing the antioxidant potential of Arbequina EVOO from different zones of Brazil and Spain was performed, applying a combined model of simulated digestion and cell cultures, using the Caco-2 cell line. Our results showed an increasing of total phenolic content (TPC) and antioxidant properties promoted by the in vitro digestion. Preincubating Caco-2 cells with bioaccessible fractions of oils counteracted the cytotoxic effect promoted by an oxidising agent (t-BOOH), preserving cell viability and reducing the generation of reactive oxygen species (ROS). The protective effect on ROS production was associated with the antioxidant activity (DPPH assay), but no relation with the TPC of the digested samples was found. Differences in the parameters evaluated were observed among the samples, which were related to climatic characteristics of the production zones. It was concluded that transformations during the digestive process are important for establishing the antioxidant potential of the oils. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/cf2e79c7-c12a-4971-b21d-b69e6d85db43/download |
| id | ipb_eb433c486b9eae6206ca31e9dcd4ee73 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/15379 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/15379 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Borges, Thays H. Pereira, J.A. Pereira, J.A. https://www.ciencia-id.pt/611F-80B2-A7C1 611F-80B2-A7C1 http://orcid.org/0000-0002-2260-0600 0000-0002-2260-0600 Cabrera-Vique, Carmen Seiquer, Isabel |
| publishDate | 2017 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engen_ENA physiological approach to assessing the antioxidant potential of Arbequina EVOO from different zones of Brazil and Spain was performed, applying a combined model of simulated digestion and cell cultures, using the Caco-2 cell line. Our results showed an increasing of total phenolic content (TPC) and antioxidant properties promoted by the in vitro digestion. Preincubating Caco-2 cells with bioaccessible fractions of oils counteracted the cytotoxic effect promoted by an oxidising agent (t-BOOH), preserving cell viability and reducing the generation of reactive oxygen species (ROS). The protective effect on ROS production was associated with the antioxidant activity (DPPH assay), but no relation with the TPC of the digested samples was found. Differences in the parameters evaluated were observed among the samples, which were related to climatic characteristics of the production zones. It was concluded that transformations during the digestive process are important for establishing the antioxidant potential of the oils.application/pdfen_ENStudy of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markersBorges, Thays H.PersonalPereira, J.A.DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857DSpacehttp://dspace.org/items/7932162e-a2da-4913-b00d-17babbe51857PereiraJosé AlbertoCiência IDhttps://www.ciencia-id.pt611F-80B2-A7C1ORCIDhttp://orcid.org0000-0002-2260-0600Researcher IDhttps://www.researcherid.comL-6798-2014Scopus Author IDhttps://www.scopus.com57204366348Cabrera-Vique, CarmenSeiquer, IsabelHostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf1756-4646DOIIsPartOf10.1016/j.jff.2017.07.0592018-01-31T10:00:00Z20172017-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/15379http://purl.org/coar/access_right/c_abf2open accessAntioxidant propertiesCaco-2 cellsDigestionExtra virgin olive oilPolyphenolsROS1305966 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/cf2e79c7-c12a-4971-b21d-b69e6d85db43/download |
| spellingShingle | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers Borges, Thays H. Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| status | SINGLETON |
| subject.fl_str_mv | Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| title | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| title_full | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| title_fullStr | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| title_full_unstemmed | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| title_short | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| title_sort | Study of the antioxidant potential of Arbequina extra virgin olive oils from Brazil and Spain applying combined models of simulated digestion and cell culture markers |
| topic | Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| topic_facet | Antioxidant properties Caco-2 cells Digestion Extra virgin olive oil Polyphenols ROS |
| url | http://hdl.handle.net/10198/15379 |
| visible | 1 |