Publicação
Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice
| Resumo: | Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16−/− without treatment, group II: treated HPV16−/−, group III: HPV16+/− without treatment and group IV: treated HPV16+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wildtype animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers. |
|---|---|
| Autores principais: | Medeiros-Fonseca, Beatriz |
| Outros Autores: | Mestre, V.F.; Colaço, B.; Pires, Maria João; Martins, Tânia; Costa, Rui Miguel Gil; Neuparth, Maria João; Medeiros, Rui; Moutinho, Magda S.S.; Dias, Maria Inês; Barros, Lillian; Bastos, Margarida M.S.M.; Félix, Luís; Venâncio, Carlos; Ferreira, Isabel C.F.R.; Antunes, L.M.; Oliveira, Paula A. |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173002684137472 |
|---|---|
| author | Medeiros-Fonseca, Beatriz |
| author2 | Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
| author2_role | author author author author author author author author author author author author author author author author |
| author_facet | Medeiros-Fonseca, Beatriz Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Medeiros-Fonseca, Beatriz\"},{\"Person.name\":\"Mestre, V.F.\"},{\"Person.name\":\"Colaço, B.\"},{\"Person.name\":\"Pires, Maria João\"},{\"Person.name\":\"Martins, Tânia\"},{\"Person.name\":\"Costa, Rui Miguel Gil\"},{\"Person.name\":\"Neuparth, Maria João\"},{\"Person.name\":\"Medeiros, Rui\"},{\"Person.name\":\"Moutinho, Magda S.S.\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"},{\"Person.name\":\"Bastos, Margarida M.S.M.\"},{\"Person.name\":\"Félix, Luís\"},{\"Person.name\":\"Venâncio, Carlos\"},{\"Person.name\":\"Ferreira, Isabel C.F.R.\",\"Person.identifier.orcid\":\"0000-0003-4910-4882\"},{\"Person.name\":\"Antunes, L.M.\"},{\"Person.name\":\"Oliveira, Paula A.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Medeiros-Fonseca, Beatriz Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.titles.title.fl_str_mv | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Medeiros-Fonseca, Beatriz Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Barros, Lillian Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Antunes, L.M. Oliveira, Paula A. |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/18753 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.title.fl_str_mv | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16−/− without treatment, group II: treated HPV16−/−, group III: HPV16+/− without treatment and group IV: treated HPV16+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wildtype animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/23866133-4125-4812-bfdc-df1085ca6a02/download |
| funding.funder.alternateName_str_mv | FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.identifier_str_mv | SFRH/BPD/85462/2012 UID/AGR/04033/2013 |
| funding.name_str_mv | SFRH 5876 |
| funding_str_mv | SFRH/BPD/85462/2012 info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F85462%2F2012/PT UID/AGR/04033/2013 info:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F04033%2F2013/PT |
| id | ipb_ee7ff58c7aaa49878b8081eafb104f9f |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/18753 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/18753 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Medeiros-Fonseca, Beatriz Mestre, V.F. Colaço, B. Pires, Maria João Martins, Tânia Costa, Rui Miguel Gil Neuparth, Maria João Medeiros, Rui Moutinho, Magda S.S. Dias, Maria Inês Dias, Maria Inês https://www.ciencia-id.pt/2A13-4BE6-C7CF 2A13-4BE6-C7CF http://orcid.org/0000-0001-8744-7814 0000-0001-8744-7814 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 Bastos, Margarida M.S.M. Félix, Luís Venâncio, Carlos Ferreira, Isabel C.F.R. Ferreira, Isabel C.F.R. https://www.ciencia-id.pt/9418-CF95-9919 9418-CF95-9919 http://orcid.org/0000-0003-4910-4882 0000-0003-4910-4882 Antunes, L.M. Oliveira, Paula A. |
| publishDate | 2018 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engCancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16−/− without treatment, group II: treated HPV16−/−, group III: HPV16+/− without treatment and group IV: treated HPV16+/−). Following the treatment period, animals were sacrificed and skin samples were used to classify skin lesions histologically. Toxicological parameters included hematological and biochemical blood markers, splenic and hepatic histology and hepatic oxidative stress. The extract did not prevent the progression of HPV16-induced cutaneous lesions in this model. The treated wildtype animals showed mild hepatitis, while transgenic animals suffered weight loss. However, there were no changes concerning hematological, biochemical and hepatic oxidative stress markers.application/pdfen_ENLaurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic miceMedeiros-Fonseca, BeatrizMestre, V.F.Colaço, B.Pires, Maria JoãoMartins, TâniaCosta, Rui Miguel GilNeuparth, Maria JoãoMedeiros, RuiMoutinho, Magda S.S.PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801Bastos, Margarida M.S.M.Félix, LuísVenâncio, CarlosPersonalFerreira, Isabel C.F.R.DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8DSpacehttp://dspace.org/items/bd0d1537-2e03-41fb-b27a-140af9c35db8FerreiraIsabel C.F.R.Ciência IDhttps://www.ciencia-id.pt9418-CF95-9919ORCIDhttp://orcid.org0000-0003-4910-4882Researcher IDhttps://www.researcherid.comE-8500-2013Scopus Author IDhttps://www.scopus.com36868826600Antunes, L.M.Oliveira, Paula A.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf2042-6496DOIIsPartOf10.1039/c8fo00783g2018-01-19T10:00:00Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/18753http://purl.org/coar/access_right/c_abf2open access2203788 bytesFundação para a Ciência e a TecnologiaPapilomavírus e Pteridium aquilinum: estudo da sua interacção no processo de carcinogéneseinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F85462%2F2012/PTSFRH/BPD/85462/2012SFRHCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaCentro de Investigação e de Tecnologias Agro-Ambientais e Biológicasinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F04033%2F2013/PTUID/AGR/04033/20135876Crossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/23866133-4125-4812-bfdc-df1085ca6a02/download |
| spellingShingle | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice Medeiros-Fonseca, Beatriz |
| status | SINGLETON |
| title | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| title_full | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| title_fullStr | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| title_full_unstemmed | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| title_short | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| title_sort | Laurus nobilis (laurel) aqueous leaf extract's toxicological and anti-tumor activities in HPV16-transgenic mice |
| url | http://hdl.handle.net/10198/18753 |
| visible | 1 |
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