Publicação
Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds
| Resumo: | Ceratonia siliqua L., commonly known as the carob tree is native to the Mediterranean countries and is widely known and consumed. Due to its chemical constituents, flavoring properties, and nutritional benefits, it has the potential to be of significant importance to the food industry1. The carob bean is made up of 90% pulp and 10% seeds. Those seeds are widely used in the food industry as a thickening agent (E-410). However, knowledge about phenolic composition and its relation to biological properties is scarce. This study used seeds of thirteen carob varieties harvested in Algarve, Portugal. The phenolic composition of the hydroethanolic extracts was analyzed by HPLC-DAD-ESI/MS. The antioxidant, anti-inflammatory, cytotoxic and antibacterial properties of the extracts were also analysed. The phenolic composition was analyzed by HPLC-DAD-ESI/MS. Cytotoxic activity was evaluated by the colourimetric method of sulforhodamine B. Anti-inflammatory activity was determined by inhibition of NO production in murine macrophages. The antibacterial activity was evaluated through the method of successive microdilutions and the antioxidant activity through the TBARS and CAA assays. Seventeen phenolics compounds were tentatively identified, being (Epi)catechin dimer type dimer and apigenin-O-hexosyl-pentoside the most abundant ones (3.08 – 11.67 mg/mL). All the varieties studied exhibited the capacity to inhibit TBARS formation. The extract obtained from the Gasparinha variety was the only one that inhibited the reactive oxygen species formation in the CAA assay. For the cytotoxic activity only Cavi, Cardeira and Pé Comprido varieties demonstrated the ability to inhibit the proliferation of the tumor cell lines tested, without showing a hepatotoxic effect. All extracts presented a broad-spectrum microbial growth inhibition without an efficient bactericidal power. These findings highlight Carob seed as a rich source of structurally diverse biomolecules with potential application as additives in food formulation development. However, further studies are needed to understand the correlation between phenolic compounds and the bioactive properties associated with carob seed tissues. |
|---|---|
| Autores principais: | Marcelino, Sandra Andreia |
| Outros Autores: | Mandim, Filipa; Oludemi, Taofiq; Dias, Maria Inês; Barracosa, Paulo; Prieto Lage, Miguel A.; Barros, Lillian |
| Assunto: | Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| Ano: | 2022 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172952721588224 |
|---|---|
| author | Marcelino, Sandra Andreia |
| author2 | Mandim, Filipa Oludemi, Taofiq Dias, Maria Inês Barracosa, Paulo Prieto Lage, Miguel A. Barros, Lillian |
| author2_role | author author author author author author |
| author_facet | Marcelino, Sandra Andreia Mandim, Filipa Oludemi, Taofiq Dias, Maria Inês Barracosa, Paulo Prieto Lage, Miguel A. Barros, Lillian |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Marcelino, Sandra Andreia\",\"Person.identifier.orcid\":\"0000-0003-1490-9445\"},{\"Person.name\":\"Mandim, Filipa\",\"Person.identifier.orcid\":\"0000-0002-6494-2326\"},{\"Person.name\":\"Oludemi, Taofiq\",\"Person.identifier.orcid\":\"0000-0003-2822-3532\"},{\"Person.name\":\"Dias, Maria Inês\",\"Person.identifier.orcid\":\"0000-0001-8744-7814\"},{\"Person.name\":\"Barracosa, Paulo\"},{\"Person.name\":\"Prieto Lage, Miguel A.\",\"Person.identifier.orcid\":\"0000-0002-3513-0054\"},{\"Person.name\":\"Barros, Lillian\",\"Person.identifier.orcid\":\"0000-0002-9050-5189\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Marcelino, Sandra Andreia Mandim, Filipa Oludemi, Taofiq Dias, Maria Inês Barracosa, Paulo Prieto Lage, Miguel A. Barros, Lillian |
| datacite.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2023-03-21T15:07:29Z |
| datacite.date.embargoed.fl_str_mv | 2023-03-21T15:07:29Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| datacite.titles.title.fl_str_mv | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Marcelino, Sandra Andreia Mandim, Filipa Oludemi, Taofiq Dias, Maria Inês Barracosa, Paulo Prieto Lage, Miguel A. Barros, Lillian |
| dc.date.Accepted.fl_str_mv | 2022-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2023-03-21T15:07:29Z |
| dc.date.embargoed.fl_str_mv | 2023-03-21T15:07:29Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/27909 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Portuguesa de Química |
| dc.rights.cclincense.fl_str_mv | http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| dc.title.fl_str_mv | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Ceratonia siliqua L., commonly known as the carob tree is native to the Mediterranean countries and is widely known and consumed. Due to its chemical constituents, flavoring properties, and nutritional benefits, it has the potential to be of significant importance to the food industry1. The carob bean is made up of 90% pulp and 10% seeds. Those seeds are widely used in the food industry as a thickening agent (E-410). However, knowledge about phenolic composition and its relation to biological properties is scarce. This study used seeds of thirteen carob varieties harvested in Algarve, Portugal. The phenolic composition of the hydroethanolic extracts was analyzed by HPLC-DAD-ESI/MS. The antioxidant, anti-inflammatory, cytotoxic and antibacterial properties of the extracts were also analysed. The phenolic composition was analyzed by HPLC-DAD-ESI/MS. Cytotoxic activity was evaluated by the colourimetric method of sulforhodamine B. Anti-inflammatory activity was determined by inhibition of NO production in murine macrophages. The antibacterial activity was evaluated through the method of successive microdilutions and the antioxidant activity through the TBARS and CAA assays. Seventeen phenolics compounds were tentatively identified, being (Epi)catechin dimer type dimer and apigenin-O-hexosyl-pentoside the most abundant ones (3.08 – 11.67 mg/mL). All the varieties studied exhibited the capacity to inhibit TBARS formation. The extract obtained from the Gasparinha variety was the only one that inhibited the reactive oxygen species formation in the CAA assay. For the cytotoxic activity only Cavi, Cardeira and Pé Comprido varieties demonstrated the ability to inhibit the proliferation of the tumor cell lines tested, without showing a hepatotoxic effect. All extracts presented a broad-spectrum microbial growth inhibition without an efficient bactericidal power. These findings highlight Carob seed as a rich source of structurally diverse biomolecules with potential application as additives in food formulation development. However, further studies are needed to understand the correlation between phenolic compounds and the bioactive properties associated with carob seed tissues. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/80ee2351-8fe1-4143-82b3-5f59d0a12f45/download |
| funding.funder.alternateName_str_mv | FCT FCT FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia Fundação para a Ciência e a Tecnologia |
| funding.name_str_mv | 6817 - DCRRNI ID 6817 - DCRRNI ID |
| id | ipb_f1313d76931e95d10d8d3ca2d3389fcd |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/27909 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/27909 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Marcelino, Sandra Andreia Marcelino, Sandra Andreia http://orcid.org/0000-0003-1490-9445 0000-0003-1490-9445 Mandim, Filipa Mandim, Filipa https://www.ciencia-id.pt/F318-9049-EB99 F318-9049-EB99 http://orcid.org/0000-0002-6494-2326 0000-0002-6494-2326 Oludemi, Taofiq Oludemi, Taofiq https://www.ciencia-id.pt/E318-A5CD-6626 E318-A5CD-6626 http://orcid.org/0000-0003-2822-3532 0000-0003-2822-3532 Dias, Maria Inês Dias, Maria Inês https://www.ciencia-id.pt/2A13-4BE6-C7CF 2A13-4BE6-C7CF http://orcid.org/0000-0001-8744-7814 0000-0001-8744-7814 Barracosa, Paulo Prieto Lage, Miguel A. Prieto Lage, Miguel A. https://www.ciencia-id.pt/221E-1E00-AB74 221E-1E00-AB74 http://orcid.org/0000-0002-3513-0054 0000-0002-3513-0054 Barros, Lillian Barros, Lillian https://www.ciencia-id.pt/9616-35CB-D001 9616-35CB-D001 http://orcid.org/0000-0002-9050-5189 0000-0002-9050-5189 |
| publishDate | 2022 |
| publisher.none.fl_str_mv | Sociedade Portuguesa de Química |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engSociedade Portuguesa de Químicapt_PTCeratonia siliqua L., commonly known as the carob tree is native to the Mediterranean countries and is widely known and consumed. Due to its chemical constituents, flavoring properties, and nutritional benefits, it has the potential to be of significant importance to the food industry1. The carob bean is made up of 90% pulp and 10% seeds. Those seeds are widely used in the food industry as a thickening agent (E-410). However, knowledge about phenolic composition and its relation to biological properties is scarce. This study used seeds of thirteen carob varieties harvested in Algarve, Portugal. The phenolic composition of the hydroethanolic extracts was analyzed by HPLC-DAD-ESI/MS. The antioxidant, anti-inflammatory, cytotoxic and antibacterial properties of the extracts were also analysed. The phenolic composition was analyzed by HPLC-DAD-ESI/MS. Cytotoxic activity was evaluated by the colourimetric method of sulforhodamine B. Anti-inflammatory activity was determined by inhibition of NO production in murine macrophages. The antibacterial activity was evaluated through the method of successive microdilutions and the antioxidant activity through the TBARS and CAA assays. Seventeen phenolics compounds were tentatively identified, being (Epi)catechin dimer type dimer and apigenin-O-hexosyl-pentoside the most abundant ones (3.08 – 11.67 mg/mL). All the varieties studied exhibited the capacity to inhibit TBARS formation. The extract obtained from the Gasparinha variety was the only one that inhibited the reactive oxygen species formation in the CAA assay. For the cytotoxic activity only Cavi, Cardeira and Pé Comprido varieties demonstrated the ability to inhibit the proliferation of the tumor cell lines tested, without showing a hepatotoxic effect. All extracts presented a broad-spectrum microbial growth inhibition without an efficient bactericidal power. These findings highlight Carob seed as a rich source of structurally diverse biomolecules with potential application as additives in food formulation development. However, further studies are needed to understand the correlation between phenolic compounds and the bioactive properties associated with carob seed tissues.application/pdfpt_PTGenetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seedsPersonalMarcelino, Sandra AndreiaDSpacehttp://dspace.org/items/d116a005-efaf-410f-8dae-8ded6c79b9d9DSpacehttp://dspace.org/items/d116a005-efaf-410f-8dae-8ded6c79b9d9MarcelinoSandra AndreiaORCIDhttp://orcid.org0000-0003-1490-9445PersonalMandim, FilipaDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdDSpacehttp://dspace.org/items/bcc274d4-7e6c-46a8-8e49-2752f9dbfbcdMandimFilipaCiência IDhttps://www.ciencia-id.ptF318-9049-EB99ORCIDhttp://orcid.org0000-0002-6494-2326PersonalOludemi, TaofiqDSpacehttp://dspace.org/items/74ac0f55-9896-40ec-8444-523044e80c4aDSpacehttp://dspace.org/items/74ac0f55-9896-40ec-8444-523044e80c4aOludemiTaofiqCiência IDhttps://www.ciencia-id.ptE318-A5CD-6626ORCIDhttp://orcid.org0000-0003-2822-3532PersonalDias, Maria InêsDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDSpacehttp://dspace.org/items/7ca8432b-e7f4-4116-a56e-9eba0d4403bdDiasMaria InêsCiência IDhttps://www.ciencia-id.pt2A13-4BE6-C7CFORCIDhttp://orcid.org0000-0001-8744-7814Researcher IDhttps://www.researcherid.comM-8242-2013Scopus Author IDhttps://www.scopus.com54388787000Barracosa, PauloPersonalPrieto Lage, Miguel A.DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579DSpacehttp://dspace.org/items/13260615-fd28-4b8b-9dd4-8a8466007579PrietoMiguel A.Ciência IDhttps://www.ciencia-id.pt221E-1E00-AB74ORCIDhttp://orcid.org0000-0002-3513-0054Researcher IDhttps://www.researcherid.comG-4516-2011Scopus Author IDhttps://www.scopus.com35937495700Scopus Author IDhttps://www.scopus.com57207755963PersonalBarros, LillianDSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01DSpacehttp://dspace.org/items/3af07ffe-f914-48ba-a5d5-efcf70fdce01BarrosLillianCiência IDhttps://www.ciencia-id.pt9616-35CB-D001ORCIDhttp://orcid.org0000-0002-9050-5189Researcher IDhttps://www.researcherid.comJ-3600-2013Researcher IDhttps://www.researcherid.comJ-3600-2013Scopus Author IDhttps://www.scopus.com35236343600Scopus Author IDhttps://www.scopus.com57194900801HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2023-03-21T15:07:29Z20222022-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/27909http://purl.org/coar/access_right/c_abf2open accessCeratonia siliqua L. seedsResearch Subject Categories::TECHNOLOGY::Chemical engineering::Food technology686117 bytesFundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaMountain Research Center6817 - DCRRNI IDCrossref Funder IDhttp://doi.org/10.13039/501100001871Fundação para a Ciência e a TecnologiaIntegrated strategies for the development of a novel functional beverage with sustainable production systemsCrossref Funder IDhttp://doi.org/10.13039/501100001871other research producthttp://purl.org/coar/resource_type/c_c94fconference object2022http://creativecommons.org/licenses/by/4.0/http://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/80ee2351-8fe1-4143-82b3-5f59d0a12f45/downloadLivro de Resumos do 12º Encontro Nacional de Cromatografia & XIV WARPA176176Aveiro |
| spellingShingle | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds Marcelino, Sandra Andreia Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| status | SINGLETON |
| subject.fl_str_mv | Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| title | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| title_full | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| title_fullStr | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| title_full_unstemmed | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| title_short | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| title_sort | Genetic information influence on phenolic composition and bioactivities of Ceratonia siliqua L. seeds |
| topic | Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| topic_facet | Ceratonia siliqua L. seeds Research Subject Categories::TECHNOLOGY::Chemical engineering::Food technology |
| url | http://hdl.handle.net/10198/27909 |
| visible | 1 |