Publicação
Electrochemical aptasensor array for multiple detection of human osteopontin
| Resumo: | Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall. |
|---|---|
| Autores principais: | Meirinho, Sofia G. |
| Outros Autores: | Dias, L.G.; Peres, António M.; Rodrigues, Lígia R. |
| Assunto: | Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173196833226752 |
|---|---|
| author | Meirinho, Sofia G. |
| author2 | Dias, L.G. Peres, António M. Rodrigues, Lígia R. |
| author2_role | author author author |
| author_facet | Meirinho, Sofia G. Dias, L.G. Peres, António M. Rodrigues, Lígia R. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Meirinho, Sofia G.\"},{\"Person.name\":\"Dias, L.G.\",\"Person.identifier.orcid\":\"0000-0002-1210-4259\"},{\"Person.name\":\"Peres, António M.\",\"Person.identifier.orcid\":\"0000-0001-6595-9165\"},{\"Person.name\":\"Rodrigues, Lígia R.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Meirinho, Sofia G. Dias, L.G. Peres, António M. Rodrigues, Lígia R. |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-31T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-31T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| datacite.titles.title.fl_str_mv | Electrochemical aptasensor array for multiple detection of human osteopontin |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Meirinho, Sofia G. Dias, L.G. Peres, António M. Rodrigues, Lígia R. |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-31T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-31T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/15375 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| dc.title.fl_str_mv | Electrochemical aptasensor array for multiple detection of human osteopontin |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/3dab268c-d920-4bfb-a877-9be255002039/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| id | ipb_f1d6a2ec50a89ee6b400a7f07da638cf |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/15375 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/15375 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Meirinho, Sofia G. Dias, L.G. Dias, L.G. https://www.ciencia-id.pt/2F11-9092-FAAF 2F11-9092-FAAF http://orcid.org/0000-0002-1210-4259 0000-0002-1210-4259 Peres, António M. Peres, António M. https://www.ciencia-id.pt/CF16-5443-F420 CF16-5443-F420 http://orcid.org/0000-0001-6595-9165 0000-0001-6595-9165 Rodrigues, Lígia R. |
| publishDate | 2018 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engen_ENElectrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN) 6 ] 3-/4- as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall.application/pdfen_ENElectrochemical aptasensor array for multiple detection of human osteopontinMeirinho, Sofia G.PersonalDias, L.G.DSpacehttp://dspace.org/items/eac8c166-4056-4ed0-8d8d-7ecb2c4481a5DSpacehttp://dspace.org/items/eac8c166-4056-4ed0-8d8d-7ecb2c4481a5DiasLuís G.Ciência IDhttps://www.ciencia-id.pt2F11-9092-FAAFORCIDhttp://orcid.org0000-0002-1210-4259Scopus Author IDhttps://www.scopus.com23569169900PersonalPeres, António M.DSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbDSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbPeresAntónio M.Ciência IDhttps://www.ciencia-id.ptCF16-5443-F420ORCIDhttp://orcid.org0000-0001-6595-9165Researcher IDhttps://www.researcherid.comI-8470-2012Scopus Author IDhttps://www.scopus.com7102331969Rodrigues, Lígia R.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf0872-1904DOIIsPartOf10.4152/pea.2018010012018-01-31T10:00:00Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/15375http://purl.org/coar/access_right/c_abf2open accessAptamerAptasensor arrayDual-screen-printed gold electrodeElectrochemical detectionOsteopontin158500 bytesFundação para a Ciência e a TecnologiaDEVELOPMENT OF A NOVEL APTAMER-BASED MULTI-SENSOR DEVICE FOR THE DETECTION OF OSTEOPONTINCrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/3dab268c-d920-4bfb-a877-9be255002039/download |
| spellingShingle | Electrochemical aptasensor array for multiple detection of human osteopontin Meirinho, Sofia G. Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| status | SINGLETON |
| subject.fl_str_mv | Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| title | Electrochemical aptasensor array for multiple detection of human osteopontin |
| title_full | Electrochemical aptasensor array for multiple detection of human osteopontin |
| title_fullStr | Electrochemical aptasensor array for multiple detection of human osteopontin |
| title_full_unstemmed | Electrochemical aptasensor array for multiple detection of human osteopontin |
| title_short | Electrochemical aptasensor array for multiple detection of human osteopontin |
| title_sort | Electrochemical aptasensor array for multiple detection of human osteopontin |
| topic | Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| topic_facet | Aptamer Aptasensor array Dual-screen-printed gold electrode Electrochemical detection Osteopontin |
| url | http://hdl.handle.net/10198/15375 |
| visible | 1 |