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A polyphasic approach for black Aspergillus identification using MALDI-TOF MS.

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Resumo:The Aspergillus section Nigri is among the best studied fungi, having different commercial applications, but also causing biodeterioration of commodities and food spoilage. Although being well studied, their identification is not straightforward, and, recently, new species have been described in this section1,2,3. These new species were not only separated from their relatives in the section by morphological distinction but also from molecular point of view. The concept of species is clearly abstract and delimitations are very difficult, and often not consensual. Recently, Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) has been used to generate spectrum of protein masses in a range of 2000 to 20000 Da that is a taxon specific fingerprinting4. This approach could be employed as an alternative to the more tedious molecular techniques.
Autores principais:Rodrigues, Paula
Outros Autores:Soares, Célia; Santos, Cledir; Fraga, Marcelo; Venâncio, Armando; Lima, Nelson
Assunto:Black aspergilli Identification
Ano:2009
País:Portugal
Tipo de documento:póster em conferência
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB

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