Publicação
Thymus citriodorus as a source of antioxidants
| Resumo: | Thymus species are well known as medicinal plants because of their biological and pharmacological properties, which include anti-asthmatic, anti-septic, antimicrobial and antioxidant. It is believed that part of these beneficial effects are due to the volatile constituents of Thymus, and thus, their essential oil composition has been the focus of many investigations. In contrast, there is only a limited number of data on the composition of other bioactive phytochemicals of Thymus and their potential biological effects. The present study aims to elucidate the phenolic composition of an ethanolic extract of Thymus citriodorus, as well as to determine its antioxidant capacity. The ethanolic extract was obtained by solubilisation of the defatted-dried plant with aqueous ethanol (80%) for twenty minutes, in a total number of five extractions. The total phenolic compounds in the extract accounted for 139±14 mg/g, as expressed as gallic acid equivalents. Further analysis of the ethanolic extract by high performance liquid chromatography (HPLC) and electrospray mass spectrometry in the negative mode allowed to conclude that its main phenolic components were rosmarinic acid (14.0±0.8 μg/mg extract), luteolin-7-O-glucoside (11±2 μg/mg extract), an apigenin derivative (9±2 μg/mg extract), eriodictyol-O-glucoside (5.5±0.7 μg/mg extract) and naringenin-O-glucoside (1.6±0.1 μg/mg extract). Moreover, the ethanolic extract of Thymus citriodorus exhibited a high antioxidative capacity, with EC50 values of 0.32±0.05 mg/ml for the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging potential and EC50 values of 0.8±0.2 mg/ml for the reducing power. Overall, these results suggest that Thymus citriodorus can be a good source of natural antioxidants. |
|---|---|
| Autores principais: | Pereira, Olívia R. |
| Outros Autores: | Peres, António M.; Domingues, Maria R.M.; Cardoso, Susana M. |
| Assunto: | Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| Ano: | 2010 |
| País: | Portugal |
| Tipo de documento: | documento de conferência |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1863850984281735168 |
|---|---|
| author | Pereira, Olívia R. |
| author2 | Peres, António M. Domingues, Maria R.M. Cardoso, Susana M. |
| author2_role | author author author |
| author_facet | Pereira, Olívia R. Peres, António M. Domingues, Maria R.M. Cardoso, Susana M. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_str | [{\"Person.name\":\"Pereira, Olívia R.\",\"Person.identifier.orcid\":\"0000-0002-6275-3134\"},{\"Person.name\":\"Peres, António M.\",\"Person.identifier.orcid\":\"0000-0001-6595-9165\"},{\"Person.name\":\"Domingues, Maria R.M.\"},{\"Person.name\":\"Cardoso, Susana M.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Pereira, Olívia R. Peres, António M. Domingues, Maria R.M. Cardoso, Susana M. |
| datacite.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2011-05-19T14:21:01Z |
| datacite.date.embargoed.fl_str_mv | 2011-05-19T14:21:01Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| datacite.titles.title.fl_str_mv | Thymus citriodorus as a source of antioxidants |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Pereira, Olívia R. Peres, António M. Domingues, Maria R.M. Cardoso, Susana M. |
| dc.date.Accepted.fl_str_mv | 2010-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2011-05-19T14:21:01Z |
| dc.date.embargoed.fl_str_mv | 2011-05-19T14:21:01Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/4410 |
| dc.language.none.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | European Federation of Food Science & Technology |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| dc.title.fl_str_mv | Thymus citriodorus as a source of antioxidants |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_c94f |
| description | Thymus species are well known as medicinal plants because of their biological and pharmacological properties, which include anti-asthmatic, anti-septic, antimicrobial and antioxidant. It is believed that part of these beneficial effects are due to the volatile constituents of Thymus, and thus, their essential oil composition has been the focus of many investigations. In contrast, there is only a limited number of data on the composition of other bioactive phytochemicals of Thymus and their potential biological effects. The present study aims to elucidate the phenolic composition of an ethanolic extract of Thymus citriodorus, as well as to determine its antioxidant capacity. The ethanolic extract was obtained by solubilisation of the defatted-dried plant with aqueous ethanol (80%) for twenty minutes, in a total number of five extractions. The total phenolic compounds in the extract accounted for 139±14 mg/g, as expressed as gallic acid equivalents. Further analysis of the ethanolic extract by high performance liquid chromatography (HPLC) and electrospray mass spectrometry in the negative mode allowed to conclude that its main phenolic components were rosmarinic acid (14.0±0.8 μg/mg extract), luteolin-7-O-glucoside (11±2 μg/mg extract), an apigenin derivative (9±2 μg/mg extract), eriodictyol-O-glucoside (5.5±0.7 μg/mg extract) and naringenin-O-glucoside (1.6±0.1 μg/mg extract). Moreover, the ethanolic extract of Thymus citriodorus exhibited a high antioxidative capacity, with EC50 values of 0.32±0.05 mg/ml for the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging potential and EC50 values of 0.8±0.2 mg/ml for the reducing power. Overall, these results suggest that Thymus citriodorus can be a good source of natural antioxidants. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | conferenceObject |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/4b091d3b-b020-4e81-bc50-85069c78fd05/download |
| id | ipb_f52a9d70a44a5e4e2a033368499df66e |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/4410 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/4410 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Pereira, Olívia R. Pereira, Olívia R. https://www.ciencia-id.pt/B312-AF21-D2FA B312-AF21-D2FA http://orcid.org/0000-0002-6275-3134 0000-0002-6275-3134 Peres, António M. Peres, António M. https://www.ciencia-id.pt/CF16-5443-F420 CF16-5443-F420 http://orcid.org/0000-0001-6595-9165 0000-0001-6595-9165 Domingues, Maria R.M. Cardoso, Susana M. |
| publishDate | 2010 |
| publisher.none.fl_str_mv | European Federation of Food Science & Technology |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engEuropean Federation of Food Science & TechnologyporThymus species are well known as medicinal plants because of their biological and pharmacological properties, which include anti-asthmatic, anti-septic, antimicrobial and antioxidant. It is believed that part of these beneficial effects are due to the volatile constituents of Thymus, and thus, their essential oil composition has been the focus of many investigations. In contrast, there is only a limited number of data on the composition of other bioactive phytochemicals of Thymus and their potential biological effects. The present study aims to elucidate the phenolic composition of an ethanolic extract of Thymus citriodorus, as well as to determine its antioxidant capacity. The ethanolic extract was obtained by solubilisation of the defatted-dried plant with aqueous ethanol (80%) for twenty minutes, in a total number of five extractions. The total phenolic compounds in the extract accounted for 139±14 mg/g, as expressed as gallic acid equivalents. Further analysis of the ethanolic extract by high performance liquid chromatography (HPLC) and electrospray mass spectrometry in the negative mode allowed to conclude that its main phenolic components were rosmarinic acid (14.0±0.8 μg/mg extract), luteolin-7-O-glucoside (11±2 μg/mg extract), an apigenin derivative (9±2 μg/mg extract), eriodictyol-O-glucoside (5.5±0.7 μg/mg extract) and naringenin-O-glucoside (1.6±0.1 μg/mg extract). Moreover, the ethanolic extract of Thymus citriodorus exhibited a high antioxidative capacity, with EC50 values of 0.32±0.05 mg/ml for the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging potential and EC50 values of 0.8±0.2 mg/ml for the reducing power. Overall, these results suggest that Thymus citriodorus can be a good source of natural antioxidants.application/pdfporThymus citriodorus as a source of antioxidantsPersonalPereira, Olívia R.DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453DSpacehttp://dspace.org/items/e8fdab9a-3ad6-47e6-b90c-7a37aabcb453PereiraOlívia R.Ciência IDhttps://www.ciencia-id.ptB312-AF21-D2FAORCIDhttp://orcid.org0000-0002-6275-3134Researcher IDhttps://www.researcherid.comH-1049-2014Scopus Author IDhttps://www.scopus.com52264387000PersonalPeres, António M.DSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbDSpacehttp://dspace.org/items/7d93be47-8dc4-4413-9304-5b978773d3bbPeresAntónio M.Ciência IDhttps://www.ciencia-id.ptCF16-5443-F420ORCIDhttp://orcid.org0000-0001-6595-9165Researcher IDhttps://www.researcherid.comI-8470-2012Scopus Author IDhttps://www.scopus.com7102331969Domingues, Maria R.M.Cardoso, Susana M.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.pt2011-05-19T14:21:01Z20102010-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/4410http://purl.org/coar/access_right/c_abf2open accessThymus citriodorusPhenolic compoundsAntioxidant activityHPLC-DADESI-MSn2509814 bytesother research producthttp://purl.org/coar/resource_type/c_c94fconference objecthttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/4b091d3b-b020-4e81-bc50-85069c78fd05/downloadEuropean Federation of Food Science & Technology 2010Dublin, Ireland |
| spellingShingle | Thymus citriodorus as a source of antioxidants Pereira, Olívia R. Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| subject.fl_str_mv | Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| title | Thymus citriodorus as a source of antioxidants |
| title_full | Thymus citriodorus as a source of antioxidants |
| title_fullStr | Thymus citriodorus as a source of antioxidants |
| title_full_unstemmed | Thymus citriodorus as a source of antioxidants |
| title_short | Thymus citriodorus as a source of antioxidants |
| title_sort | Thymus citriodorus as a source of antioxidants |
| topic | Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| topic_facet | Thymus citriodorus Phenolic compounds Antioxidant activity HPLC-DAD ESI-MSn |
| url | http://hdl.handle.net/10198/4410 |
| visible | 1 |