Publicação
Red blood cells (RBCs) visualisation in bifurcations and bends
| Resumo: | Bifurcating networks are commonly found in nature. One example is the microvascular system, composed of blood vessels consecutively branching into daughter vessels, driving the blood into the capillaries, where the red blood cells (RBCs) are responsible for delivering O 2 and up taking cell waste and CO 2 . In this preliminary study, we explore a microfluidic bifurcating geometry inspired by such biological models, for investigating RBC partitioning as well as RBC-plasma separation favored by the consecutive bifurcating channels. A biomimetic design rule [1] based on Murray’s law [2] was used to set the channels’ dimensions along the network, which consists of consecutive bifurcating channels of reducing diameter. The ability to apply differential flow resistances by controlling the flow rates at the end of the network allowed us to monitor the formation of a cell-free layer (CFL) for different flow conditions at haematocrits of 1% and 5%. We have also compared the values of CFL thickness determined directly by the measurement on the projection image created from a stack of images or indirectly by analyzing the intensity profile in the same projection. The results obtained from this study confirm the potential to study RBC partitioning along bifurcating networks, which could be of particular interest for the separation of RBCs from plasma in point-of-care devices. |
|---|---|
| Autores principais: | Fidalgo, Joana |
| Outros Autores: | Pinho, Diana; Lima, Rui A.; Oliveira, Mónica S.N. |
| Assunto: | Bifurcating networks Cell-free layer Microcirculation RBC |
| Ano: | 2018 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867172827548876800 |
|---|---|
| author | Fidalgo, Joana |
| author2 | Pinho, Diana Lima, Rui A. Oliveira, Mónica S.N. |
| author2_role | author author author |
| author_facet | Fidalgo, Joana Pinho, Diana Lima, Rui A. Oliveira, Mónica S.N. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Fidalgo, Joana\"},{\"Person.name\":\"Pinho, Diana\",\"Person.identifier.orcid\":\"0000-0002-3884-6496\"},{\"Person.name\":\"Lima, Rui A.\"},{\"Person.name\":\"Oliveira, Mónica S.N.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Fidalgo, Joana Pinho, Diana Lima, Rui A. Oliveira, Mónica S.N. |
| datacite.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-31T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-31T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Bifurcating networks Cell-free layer Microcirculation RBC |
| datacite.titles.title.fl_str_mv | Red blood cells (RBCs) visualisation in bifurcations and bends |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Fidalgo, Joana Pinho, Diana Lima, Rui A. Oliveira, Mónica S.N. |
| dc.date.Accepted.fl_str_mv | 2018-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-31T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-31T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/15399 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Bifurcating networks Cell-free layer Microcirculation RBC |
| dc.title.fl_str_mv | Red blood cells (RBCs) visualisation in bifurcations and bends |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Bifurcating networks are commonly found in nature. One example is the microvascular system, composed of blood vessels consecutively branching into daughter vessels, driving the blood into the capillaries, where the red blood cells (RBCs) are responsible for delivering O 2 and up taking cell waste and CO 2 . In this preliminary study, we explore a microfluidic bifurcating geometry inspired by such biological models, for investigating RBC partitioning as well as RBC-plasma separation favored by the consecutive bifurcating channels. A biomimetic design rule [1] based on Murray’s law [2] was used to set the channels’ dimensions along the network, which consists of consecutive bifurcating channels of reducing diameter. The ability to apply differential flow resistances by controlling the flow rates at the end of the network allowed us to monitor the formation of a cell-free layer (CFL) for different flow conditions at haematocrits of 1% and 5%. We have also compared the values of CFL thickness determined directly by the measurement on the projection image created from a stack of images or indirectly by analyzing the intensity profile in the same projection. The results obtained from this study confirm the potential to study RBC partitioning along bifurcating networks, which could be of particular interest for the separation of RBCs from plasma in point-of-care devices. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/840cc37b-cb12-4396-8f9e-314739d4e057/download |
| id | ipb_f7db7491e066550bc76e8a293cfafce5 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/15399 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/15399 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Fidalgo, Joana Pinho, Diana Pinho, Diana https://www.ciencia-id.pt/5214-ED0C-35E8 5214-ED0C-35E8 http://orcid.org/0000-0002-3884-6496 0000-0002-3884-6496 Lima, Rui A. Oliveira, Mónica S.N. |
| publishDate | 2018 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engen_ENBifurcating networks are commonly found in nature. One example is the microvascular system, composed of blood vessels consecutively branching into daughter vessels, driving the blood into the capillaries, where the red blood cells (RBCs) are responsible for delivering O 2 and up taking cell waste and CO 2 . In this preliminary study, we explore a microfluidic bifurcating geometry inspired by such biological models, for investigating RBC partitioning as well as RBC-plasma separation favored by the consecutive bifurcating channels. A biomimetic design rule [1] based on Murray’s law [2] was used to set the channels’ dimensions along the network, which consists of consecutive bifurcating channels of reducing diameter. The ability to apply differential flow resistances by controlling the flow rates at the end of the network allowed us to monitor the formation of a cell-free layer (CFL) for different flow conditions at haematocrits of 1% and 5%. We have also compared the values of CFL thickness determined directly by the measurement on the projection image created from a stack of images or indirectly by analyzing the intensity profile in the same projection. The results obtained from this study confirm the potential to study RBC partitioning along bifurcating networks, which could be of particular interest for the separation of RBCs from plasma in point-of-care devices.application/pdfen_ENRed blood cells (RBCs) visualisation in bifurcations and bendsFidalgo, JoanaPersonalPinho, DianaDSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451DSpacehttp://dspace.org/items/02bf2dd9-33ed-4d87-b61d-ca39c51f8451PinhoDianaCiência IDhttps://www.ciencia-id.pt5214-ED0C-35E8ORCIDhttp://orcid.org0000-0002-3884-6496Lima, Rui A.Oliveira, Mónica S.N.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptISSNIsPartOf22129-391DOIIsPartOf10.1007/978-3-319-68195-5_1032018-01-31T10:00:00Z20182018-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/15399http://purl.org/coar/access_right/c_abf2open accessBifurcating networksCell-free layerMicrocirculationRBC2272904 bytesliteraturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/840cc37b-cb12-4396-8f9e-314739d4e057/download |
| spellingShingle | Red blood cells (RBCs) visualisation in bifurcations and bends Fidalgo, Joana Bifurcating networks Cell-free layer Microcirculation RBC |
| status | SINGLETON |
| subject.fl_str_mv | Bifurcating networks Cell-free layer Microcirculation RBC |
| title | Red blood cells (RBCs) visualisation in bifurcations and bends |
| title_full | Red blood cells (RBCs) visualisation in bifurcations and bends |
| title_fullStr | Red blood cells (RBCs) visualisation in bifurcations and bends |
| title_full_unstemmed | Red blood cells (RBCs) visualisation in bifurcations and bends |
| title_short | Red blood cells (RBCs) visualisation in bifurcations and bends |
| title_sort | Red blood cells (RBCs) visualisation in bifurcations and bends |
| topic | Bifurcating networks Cell-free layer Microcirculation RBC |
| topic_facet | Bifurcating networks Cell-free layer Microcirculation RBC |
| url | http://hdl.handle.net/10198/15399 |
| visible | 1 |