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Potential of plant extracts in targeting SARS-CoV-2 main protease: an in vitro and in silico study

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Resumo:The deaths caused by the covid-19 pandemic have recently decreased due to a worldwide effort in vaccination campaigns. However, even vaccinated people can develop a severe form of the disease that requires ICU admission. As a result, the search for antiviral drugs to treat these severe cases has become a necessity. In this context, natural products are an interesting alternative to synthetic medicines used in drug repositioning, as they have been consumed for a long time through traditional medicine. Many natural compounds found in plant extracts have already been shown to be effective in treating viral and bacterial diseases, making them possible hits to exploit against covid-19. The objective of this work was to evaluate the antiviral activity of different plant extracts available in the library of natural products of the Universidade Estadual de Maringa, by inhibiting the SARS-CoV-2 main protease (M-pro), and by preventing viral infection in a cellular model. As a result, the extract of Cytinus hypocistis, obtained by ultrasound, showed a M-pro inhibition capacity greater than 90%. In the infection model assays using Vero cells, an inhibition of 99.6% was observed, with a selectivity index of 42.7. The in silico molecular docking simulations using the extract compounds against M-pro, suggested Tellimagrandin II as the component of C. hypocistis extract most likely to inhibit the viral enzyme. These results demonstrate the potential of C. hypocistis extract as a promising source of natural compounds with antiviral activity against covid-19.
Autores principais:Araujo, Ingrid Garcia de
Outros Autores:Pattaro-Junior, Jose Renato; Barbosa, Cecilia Gomes; Philippsen, Gisele Strieder; Silva, Ana Rita; Ioshino, Rafaella Sayuri; Moraes, Carolina Borsoi; Freitas-Junior, Lucio Holanda; Barros, Lillian; Peralta, Rosane M.; Fernandez, Maria Aparecida; Seixas, Flávio Augusto Vicente
Assunto:SARS-CoV-2 Covid-19 Cytinus hypocistis Tannins Tellimagrandin II
Ano:2023
País:Portugal
Tipo de documento:artigo
Tipo de acesso:acesso aberto
Instituição associada:Instituto Politécnico de Bragança
Idioma:inglês
Origem:Biblioteca Digital do IPB

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