Publicação
Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole
| Resumo: | Background: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatus was performed according to the CLSI M38-A2 protocol. In vitro induction assays were conducted involving daily incubation of susceptible A. fumigatus isolates, at 35°C and 180 rpm, in fresh GYEP broth medium supplemented with Prochloraz (PCZ), a potent agricultural antifungal, for a period of 30 days. Minimal inhibitory concentrations (MIC) of PCZ and clinical azoles were monitored every ten days. In order to assess the stability of the developed MIC, the strains were afterwards sub-cultured for an additional 30 days in the absence of antifungal. Along the in vitro induction process, microscopic and macroscopic cultural observations were registered. Results: MIC of PCZ increased 256 times after the initial exposure; cross-resistance to all tested clinical azoles was observed. The new MIC value of agricultural and of clinical azoles maintained stable in the absence of the selective PCZ pressure. PCZ exposure was also associated to morphological colony changes: macroscopically the colonies became mostly white, losing the typical pigmentation; microscopic examination revealed the absence of conidiation. Conclusions: PCZ exposure induced Aspergillus fumigatus morphological changes and an evident increase of MIC value to PCZ as well as the development of cross-resistance with posaconazole, itraconazole and voriconazole. |
|---|---|
| Autores principais: | Faria-Ramos, Isabel |
| Outros Autores: | Farinha, Sofia; Neves-Maia, João; Tavares, Pedro Ribeiro; Miranda, Isabel M.; Estevinho, Leticia M.; Pina-Vaz, Cidália; Rodrigues, Acácio G. |
| Assunto: | Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| Ano: | 2014 |
| País: | Portugal |
| Tipo de documento: | artigo |
| Tipo de acesso: | acesso aberto |
| Instituição associada: | Instituto Politécnico de Bragança |
| Idioma: | inglês |
| Origem: | Biblioteca Digital do IPB |
| _version_ | 1867173173470953472 |
|---|---|
| author | Faria-Ramos, Isabel |
| author2 | Farinha, Sofia Neves-Maia, João Tavares, Pedro Ribeiro Miranda, Isabel M. Estevinho, Leticia M. Pina-Vaz, Cidália Rodrigues, Acácio G. |
| author2_role | author author author author author author author |
| author_facet | Faria-Ramos, Isabel Farinha, Sofia Neves-Maia, João Tavares, Pedro Ribeiro Miranda, Isabel M. Estevinho, Leticia M. Pina-Vaz, Cidália Rodrigues, Acácio G. |
| author_role | author |
| contributor_name_str_mv | Biblioteca Digital do IPB |
| country_str | PT |
| creators_json_txt | [{\"Person.name\":\"Faria-Ramos, Isabel\"},{\"Person.name\":\"Farinha, Sofia\"},{\"Person.name\":\"Neves-Maia, João\"},{\"Person.name\":\"Tavares, Pedro Ribeiro\"},{\"Person.name\":\"Miranda, Isabel M.\"},{\"Person.name\":\"Estevinho, Leticia M.\",\"Person.identifier.orcid\":\"0000-0002-9249-1948\"},{\"Person.name\":\"Pina-Vaz, Cidália\"},{\"Person.name\":\"Rodrigues, Acácio G.\"}] |
| datacite.contributors.contributor.contributorName.fl_str_mv | Biblioteca Digital do IPB |
| datacite.creators.creator.creatorName.fl_str_mv | Faria-Ramos, Isabel Farinha, Sofia Neves-Maia, João Tavares, Pedro Ribeiro Miranda, Isabel M. Estevinho, Leticia M. Pina-Vaz, Cidália Rodrigues, Acácio G. |
| datacite.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| datacite.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| datacite.rights.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| datacite.subjects.subject.fl_str_mv | Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| datacite.titles.title.fl_str_mv | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| dc.contributor.none.fl_str_mv | Biblioteca Digital do IPB |
| dc.creator.none.fl_str_mv | Faria-Ramos, Isabel Farinha, Sofia Neves-Maia, João Tavares, Pedro Ribeiro Miranda, Isabel M. Estevinho, Leticia M. Pina-Vaz, Cidália Rodrigues, Acácio G. |
| dc.date.Accepted.fl_str_mv | 2014-01-01T00:00:00Z |
| dc.date.available.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.date.embargoed.fl_str_mv | 2018-01-19T10:00:00Z |
| dc.format.none.fl_str_mv | application/pdf |
| dc.identifier.none.fl_str_mv | http://hdl.handle.net/10198/16212 |
| dc.language.none.fl_str_mv | eng |
| dc.rights.none.fl_str_mv | http://purl.org/coar/access_right/c_abf2 |
| dc.subject.none.fl_str_mv | Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| dc.title.fl_str_mv | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| dc.type.none.fl_str_mv | http://purl.org/coar/resource_type/c_6501 |
| description | Background: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatus was performed according to the CLSI M38-A2 protocol. In vitro induction assays were conducted involving daily incubation of susceptible A. fumigatus isolates, at 35°C and 180 rpm, in fresh GYEP broth medium supplemented with Prochloraz (PCZ), a potent agricultural antifungal, for a period of 30 days. Minimal inhibitory concentrations (MIC) of PCZ and clinical azoles were monitored every ten days. In order to assess the stability of the developed MIC, the strains were afterwards sub-cultured for an additional 30 days in the absence of antifungal. Along the in vitro induction process, microscopic and macroscopic cultural observations were registered. Results: MIC of PCZ increased 256 times after the initial exposure; cross-resistance to all tested clinical azoles was observed. The new MIC value of agricultural and of clinical azoles maintained stable in the absence of the selective PCZ pressure. PCZ exposure was also associated to morphological colony changes: macroscopically the colonies became mostly white, losing the typical pigmentation; microscopic examination revealed the absence of conidiation. Conclusions: PCZ exposure induced Aspergillus fumigatus morphological changes and an evident increase of MIC value to PCZ as well as the development of cross-resistance with posaconazole, itraconazole and voriconazole. |
| dirty | 0 |
| eu_rights_str_mv | openAccess |
| format | article |
| fulltext.url.fl_str_mv | https://bibliotecadigital.ipb.pt/bitstreams/6ee2b9cc-92c1-4cb2-989d-1af9a16d96d9/download |
| funding.funder.alternateName_str_mv | FCT |
| funding.funder.identifier_str_mv | http://doi.org/10.13039/501100001871 |
| funding.funder.name_str_mv | Fundação para a Ciência e a Tecnologia |
| id | ipb_fc70db13eea7f28020bccde1d3d8e022 |
| identifier.url.fl_str_mv | http://hdl.handle.net/10198/16212 |
| instacron_str | ipb |
| institution | Instituto Politécnico de Bragança |
| instname_str | Instituto Politécnico de Bragança |
| language | eng |
| network_acronym_str | ipb |
| network_name_str | Biblioteca Digital do IPB |
| oai_identifier_str | oai:bibliotecadigital.ipb.pt:10198/16212 |
| organization_str_mv | urn:organizationAcronym:ipb |
| person_str_mv | Faria-Ramos, Isabel Farinha, Sofia Neves-Maia, João Tavares, Pedro Ribeiro Miranda, Isabel M. Estevinho, Leticia M. Estevinho, Leticia M. https://www.ciencia-id.pt/BA14-09D6-A406 BA14-09D6-A406 http://orcid.org/0000-0002-9249-1948 0000-0002-9249-1948 Pina-Vaz, Cidália Rodrigues, Acácio G. |
| publishDate | 2014 |
| reponame_str | Biblioteca Digital do IPB |
| repository_id_str | urn:repositoryAcronym:ipb |
| service_str_mv | urn:repositoryAcronym:ipb |
| spelling | engen_ENBackground: The purpose of this study was to unveil whether azole antifungals used in agriculture, similar to the clinical azoles used in humans, can evoke resistance among relevant human pathogens like Aspergillus fumigatus, an ubiquitous agent in nature. Additionally, cross-resistance with clinical azoles was investigated. Antifungal susceptibility testing of environmental and clinical isolates of A. fumigatus was performed according to the CLSI M38-A2 protocol. In vitro induction assays were conducted involving daily incubation of susceptible A. fumigatus isolates, at 35°C and 180 rpm, in fresh GYEP broth medium supplemented with Prochloraz (PCZ), a potent agricultural antifungal, for a period of 30 days. Minimal inhibitory concentrations (MIC) of PCZ and clinical azoles were monitored every ten days. In order to assess the stability of the developed MIC, the strains were afterwards sub-cultured for an additional 30 days in the absence of antifungal. Along the in vitro induction process, microscopic and macroscopic cultural observations were registered. Results: MIC of PCZ increased 256 times after the initial exposure; cross-resistance to all tested clinical azoles was observed. The new MIC value of agricultural and of clinical azoles maintained stable in the absence of the selective PCZ pressure. PCZ exposure was also associated to morphological colony changes: macroscopically the colonies became mostly white, losing the typical pigmentation; microscopic examination revealed the absence of conidiation. Conclusions: PCZ exposure induced Aspergillus fumigatus morphological changes and an evident increase of MIC value to PCZ as well as the development of cross-resistance with posaconazole, itraconazole and voriconazole.application/pdfen_ENDevelopment of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azoleFaria-Ramos, IsabelFarinha, SofiaNeves-Maia, JoãoTavares, Pedro RibeiroMiranda, Isabel M.PersonalEstevinho, Leticia M.DSpacehttp://dspace.org/items/4a1d5ba2-1854-4ca5-89a4-73f35e964df9DSpacehttp://dspace.org/items/4a1d5ba2-1854-4ca5-89a4-73f35e964df9EstevinhoLetícia M.Ciência IDhttps://www.ciencia-id.ptBA14-09D6-A406ORCIDhttp://orcid.org0000-0002-9249-1948Scopus Author IDhttps://www.scopus.com6506577664Scopus Author IDhttps://www.scopus.com57211945650Pina-Vaz, CidáliaRodrigues, Acácio G.HostingInstitutionOrganizationalBiblioteca Digital do IPBe-mailmailto:dspace@ipb.ptdspace@ipb.ptDOIIsPartOf10.1186/1471-2180-14-1552018-01-19T10:00:00Z20142014-01-01T00:00:00ZHandlehttp://hdl.handle.net/10198/16212http://purl.org/coar/access_right/c_abf2open accessAspergillus fumigatusClinical and agricultural azolesCross-resistance582136 bytesFundação para a Ciência e a TecnologiaEMERGENCE OF MULTIDRUG CROSS-RESISTANCE BETWEEN AGRICULTURAL AND HUMAN ANTIFUNGALS IN CLINICALLY RELEVANT SPECIES OF ASPERGILLUS AND CANDIDACrossref Funder IDhttp://doi.org/10.13039/501100001871literaturehttp://purl.org/coar/resource_type/c_6501journal articlehttp://purl.org/coar/access_right/c_abf2application/pdffulltexthttps://bibliotecadigital.ipb.pt/bitstreams/6ee2b9cc-92c1-4cb2-989d-1af9a16d96d9/download |
| spellingShingle | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole Faria-Ramos, Isabel Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| status | SINGLETON |
| subject.fl_str_mv | Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| title | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| title_full | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| title_fullStr | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| title_full_unstemmed | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| title_short | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| title_sort | Development of cross-resistance by Aspergillus fumigatus to clinical azoles following exposure to prochloraz, an agricultural azole |
| topic | Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| topic_facet | Aspergillus fumigatus Clinical and agricultural azoles Cross-resistance |
| url | http://hdl.handle.net/10198/16212 |
| visible | 1 |